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公开(公告)号：US06383811B2
公开(公告)日：2002-05-07
申请号：US09753990
申请日：2001-01-02
IPC分类号： A61K9127
CPC分类号： C12N15/88 , A61K47/58 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041 , C12N15/87
摘要： An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
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公开(公告)号：US07491538B2
公开(公告)日：2009-02-17
申请号：US11189321
申请日：2005-07-26
IPC分类号： C12N15/88
摘要： A process and compound wherein nucleic acids can be modified with a host of molecules and maintain their ability to be expressed. A modifying chemical attachment of polyions to polynucleotides can be used to facilitate the change of tertiary structure of the nucleic acid and in some cases condensation of nucleic acids into smaller, charged particles useful in delivering the nucleic acid to a cell.
摘要翻译： 一种方法和化合物，其中核酸可以用多种分子修饰并保持其表达能力。 可以将聚阴离子与多核苷酸的修饰化学连接用于促进核酸的三级结构的改变，并且在一些情况下，将核酸缩合成用于将核酸递送至细胞的较小带电粒子。
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公开(公告)号：US07144869B2
公开(公告)日：2006-12-05
申请号：US10310398
申请日：2002-12-05
CPC分类号： A61K48/0075
摘要： Processes are described for obtaining high levels of gene expression in primates after injection of nucleic acid to the liver via the lumen of the hepatic vein. The described process results in high level of gene expression with transient increases in liver enzymes.
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公开(公告)号：US07049144B2
公开(公告)日：2006-05-23
申请号：US10004763
申请日：2001-12-05
申请人： Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , Vladimir S. Trubetskoy , Paul M. Slattum , Lisa J. Hanson
发明人： Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , Vladimir S. Trubetskoy , Paul M. Slattum , Lisa J. Hanson
IPC分类号： C12N15/88
CPC分类号： C12N15/87 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041
摘要： A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
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公开(公告)号：US06740336B2
公开(公告)日：2004-05-25
申请号：US10265024
申请日：2002-10-04
申请人： Vladimir S. Trubetskoy , Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , So Chun Wong , Jason Klein
发明人： Vladimir S. Trubetskoy , Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , So Chun Wong , Jason Klein
IPC分类号： A61K9127
CPC分类号： A61K48/0008
摘要： We describe a process for generating multilayer particles comprising condensing a polymer with an oppositely charged polymer to form a particle and sequentially adding oppositely charged polymers to the particle forming at least three layers of polymers. The process is used to form a composition for delivering a biologically active compound to a cell.
摘要翻译： 我们描述了一种产生多层颗粒的方法，包括将聚合物与带相反电荷的聚合物缩合形成颗粒，并依次向形成至少三层聚合物的颗粒中加入带相反电荷的聚合物。 该方法用于形成用于将生物活性化合物递送至细胞的组合物。
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公开(公告)号：US07524680B2
公开(公告)日：2009-04-28
申请号：US10095681
申请日：2002-03-11
IPC分类号： C12N15/88
CPC分类号： C12N15/88 , A61K47/58 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041 , C12N15/87
摘要： An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
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37.发明授权公开(公告)号：US07326780B2
公开(公告)日：2008-02-05
申请号：US11415021
申请日：2006-05-01
CPC分类号： C07D209/08 , C07D209/14 , C07H19/00 , C07H21/04
摘要： Compounds and methods are provided for a single-pot covalent attachment of a label to nucleic acids comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
摘要翻译： 提供了化合物和方法用于标记物与核酸的单罐共价连接，包括形成用于烷基化分子的共价连接的标记试剂。 然后，在标记试剂与分子具有反应性，从而形成共价键的条件下，将可共价连接的标记试剂与含有该分子的混合物组合。
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38.发明授权公开(公告)号：US06960690B2
公开(公告)日：2005-11-01
申请号：US10005294
申请日：2001-12-05
申请人： Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , Vladimir S. Trubetskoy , Paul M. Slattum , Lisa J. Hanson
发明人： Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , Vladimir S. Trubetskoy , Paul M. Slattum , Lisa J. Hanson
IPC分类号： A61K47/48 , A61K48/00 , C12N15/87 , C07C211/63 , C07C69/52 , C07C233/00 , C12Q1/68
CPC分类号： C12N15/87 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041
摘要： A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
摘要翻译： 使用模板聚合在核酸存在下形成聚合物的方法。 此外，在异相体系中发生聚合的方法。 这些方法可用于递送核酸，用于冷凝核酸，用于形成核酸结合聚合物，用于形成含有核酸和聚合物的超分子复合物，并用于形成内聚电解质络合物。
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公开(公告)号：US06794189B2
公开(公告)日：2004-09-21
申请号：US10095682
申请日：2002-05-10
IPC分类号： C12N1588
CPC分类号： C12N15/88 , A61K47/58 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041 , C12N15/87
摘要： An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
摘要翻译： 为了向细胞递送核酸，将聚二聚物用于缩合的多核苷酸复合物中。 络合物可以用适量的正电荷和/或负电荷形成，使得所得复合物可以递送到血管外空间并且可以进一步递送至细胞。
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公开(公告)号：US06339067B1
公开(公告)日：2002-01-15
申请号：US09000692
申请日：1997-12-30
申请人： Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , Vladimir S. Trubetskoy , Paul M. Slattum , Lisa J. Hanson
发明人： Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , Vladimir S. Trubetskoy , Paul M. Slattum , Lisa J. Hanson
IPC分类号： A61K4800
CPC分类号： C12N15/87 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041
摘要： A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
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