Method for producing yellow flower by controlling flavonoid synthetic pathway
    31.
    发明授权
    Method for producing yellow flower by controlling flavonoid synthetic pathway 失效
    通过控制黄酮合成途径生产黄花的方法

    公开(公告)号:US07750209B2

    公开(公告)日:2010-07-06

    申请号:US10583110

    申请日:2004-12-17

    CPC分类号: C12N15/825

    摘要: There is provided a gene coding for the amino acid sequence listed as SEQ ID NO: 2 or SEQ ID NO: 70, for example. Co-expression of the 4′CGT gene and AS gene in a plant lacking natural aurone synthesis ability is carried out to successfully accumulate aurones and alter the flower color to have a yellow tint. In addition to the expression of both genes, the flavonoid pigment synthesis pathway of the host plant itself is inhibited to obtain flowers with a more defined yellow color.

    摘要翻译: 提供了编码例如SEQ ID NO:2或SEQ ID NO:70所列的氨基酸序列的基因。 进行4'CGT基因和AS基因在缺乏天然鸢尾碱合成能力的植物中的共表达,以成功地积累a ones并改变花色,使其具有黄色色调。 除了两种基因的表达外,宿主植物本身的类黄酮色素合成途径被抑制以获得更明确的黄色的花。

    PERILLA-DERIVED PROMOTER FUNCTIONING IN PETALS
    34.
    发明申请
    PERILLA-DERIVED PROMOTER FUNCTIONING IN PETALS 有权
    PERILA衍生的促销剂功能在PETALS

    公开(公告)号:US20120042421A1

    公开(公告)日:2012-02-16

    申请号:US13265752

    申请日:2010-03-09

    CPC分类号: C12N15/823

    摘要: There is provided a novel promoter useful for altering flow color of plants. The present invention relates to a nucleic acid selected from the group consisting of: (1) a nucleic acid containing the nucleotide sequence indicated in SEQ ID NO. 1; (2) a nucleic acid able to function as a transcriptional regulatory region of perilla anthocyanin 3-acyltransferase, and containing a nucleotide sequence in which the nucleotide sequence indicated in SEQ ID NO. 1 has been modified by addition, deletion and/or substitution of one or several nucleotides; (3) a nucleic acid able to function as a transcriptional regulatory region of perilla anthocyanin 3-acyltransferase, and able to hybridize under high stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence indicated in SEQ ID NO. 1; and, (4) a nucleic acid able to function as a transcriptional regulatory region of perilla anthocyanin 3-acyltransferase, and having sequence identity of at least 90% with the nucleotide sequence indicated in SEQ ID NO. 1.

    摘要翻译: 提供了可用于改变植物流动颜色的新型启动子。 本发明涉及选自以下的核酸:(1)含有SEQ ID NO:1所示的核苷酸序列的核酸。 1; (2)能够用作紫苏花青素3-酰基转移酶的转录调节区的核酸,并且含有核苷酸序列,其中SEQ ID NO: 1已经通过一个或几个核苷酸的添加,缺失和/或取代被修饰; (3)能够用作紫苏花青素3-酰基转移酶的转录调节区的核酸,能够在高严格条件下与由与SEQ ID NO:1所示的核苷酸序列互补的核苷酸序列组成的核酸杂交。 1; 和(4)能够用作紫苏花青素3-酰基转移酶的转录调节区的核酸,并且与SEQ ID NO:1所示的核苷酸序列具有至少90%的序列同一性。 1。

    METHOD FOR DETERMINATION OF PRESENCE OF CROSSING WITH CULTIVATED ROSE IN WILD ROSE
    36.
    发明申请
    METHOD FOR DETERMINATION OF PRESENCE OF CROSSING WITH CULTIVATED ROSE IN WILD ROSE 失效
    用于确定在野生玫瑰中具有激发的玫瑰的交叉存在的方法

    公开(公告)号:US20100143914A1

    公开(公告)日:2010-06-10

    申请号:US12532246

    申请日:2008-03-21

    IPC分类号: C12Q1/68

    CPC分类号: C12Q1/6895 C12Q2600/156

    摘要: Disclosed is a method for determining whether or not a wild rose of interest is crossed with a cultivated rose. The method comprises the steps of: examining whether or not a KSN gene containing a transposon (an indicator) is contained in the rose of interest; and determining that the rose of interest is crossed with a cultivated rose when the individual has the transposon-containing KSN gene.

    摘要翻译: 公开了一种用于确定野生玫瑰是否与栽培的玫瑰杂交的方法。 该方法包括以下步骤:检查含有转座子(指标)的KSN基因是否包含在感兴趣的玫瑰中; 并且当个体具有含转座子的KSN基因时,确定感兴趣的玫瑰与栽培的玫瑰杂交。

    Rose plant named ‘Florirosavio04’
    37.
    植物专利
    Rose plant named ‘Florirosavio04’ 有权
    玫瑰植物名为“Florirosavio04”

    公开(公告)号:USPP23653P2

    公开(公告)日:2013-06-11

    申请号:US12925948

    申请日:2010-11-02

    IPC分类号: A01H5/00

    CPC分类号: A01H6/749 A01H5/02

    摘要: A new and distinct cultivar of Rose plant named ‘Florirosavio04’, characterized by its upright flowering stems; vigorous growth habit; freely branching plant habit; and strongly fragrant flowers that are light violet in color.

    摘要翻译: 玫瑰植物新品种名称为“Florirosavio04”,其特征是其直立的花茎; 生长习惯旺盛 自由分枝植物习性; 和强烈的芬芳的花朵是浅紫色的颜色。

    Glycosyltransferase gene
    38.
    发明授权
    Glycosyltransferase gene 失效
    糖基转移酶基因

    公开(公告)号:US07514597B2

    公开(公告)日:2009-04-07

    申请号:US10524842

    申请日:2003-08-20

    摘要: The present invention provides an enzyme which catalyzes a reaction to transfer sugar to a hydroxyl group at position 2′ of chalcones and a gene thereof, and preferably an enzyme which catalyzes a reaction to transfer glucose to a hydroxyl group at position 2′ of the chalcones. Furthermore, the invention provides a plant whose flower color has been changed using the glycosyltransferase. Using probes corresponding to conservative regions of the glycosyltransferase, some tens of glycosyltransferase genes having nucleotide sequences corresponding to the conservative regions were cloned from flower petal cDNA libraries of carnation and the like. Furthermore, each of the glycosyltransferase genes was expressed in Escherichia coli, activity to transfer glucose to position 2′ of the chalcone, i.e., the glycosyltransferase activity at position 2′ of chalcone was confirmed in an extract solution of the Escherichia coli, and it was confirmed that cloned genes encoded the glycosyltransferase at position 2′.

    摘要翻译: 本发明提供了一种酶,其催化将糖转移到胡烷酮的2'位的羟基及其基因的反应,优选催化将葡萄糖转移至查尔酮2'位羟基的反应的酶 。 此外,本发明提供了使用糖基转移酶改变花色的植物。 使用与糖基转移酶保守区相对应的探针,从康乃馨花花瓣cDNA文库克隆了数十个具有与保守区相对应的核苷酸序列的糖基转移酶基因。 此外,每个糖基转移酶基因在大肠杆菌中表达,将葡萄糖转移到查耳酮的位置2'的活性,即在大肠杆菌的提取液中证实了查耳酮的2'位上的糖基转移酶活性, 证实克隆的基因在2'位编码糖基转移酶。

    Novel glycosyltransferase gene
    39.
    发明申请
    Novel glycosyltransferase gene 失效
    新型糖基转移酶基因

    公开(公告)号:US20060174377A1

    公开(公告)日:2006-08-03

    申请号:US10524842

    申请日:2003-08-20

    摘要: The present invention provides an enzyme which catalyzes a reaction to transfer sugar to a hydroxyl group at position 2′ of chalcones and a gene thereof, and preferably an enzyme which catalyzes a reaction to transfer glucose to a hydroxyl group at position 2′ of the chalcones. Furthermore, the invention provides a plant whose flower color has been changed using the glycosyltransferase. Using probes corresponding to conservative regions of the glycosyltransferase, some tens of glycosyltransferase genes having nucleotide sequences corresponding to the conservative regions were cloned from flower petal cDNA libraries of carnation and the like. Furthermore, each of the glycosyltransferase genes was expressed in Escherichia coli, activity to transfer glucose to position 2′ of the chalcone, i.e., the glycosyltransferase activity at position 2′ of chalcone was confirmed in an extract solution of the Escherichia coli, and it was confirmed that cloned genes encoded the glycosyltransferase at position 2′.

    摘要翻译: 本发明提供了一种酶,其催化将糖转移到胡烷酮的2'位的羟基及其基因的反应,优选催化将葡萄糖转移至查尔酮2'位羟基的反应的酶 。 此外,本发明提供了使用糖基转移酶改变花色的植物。 使用与糖基转移酶保守区相对应的探针,从康乃馨花花瓣cDNA文库克隆了数十个具有与保守区相对应的核苷酸序列的糖基转移酶基因。 此外,每个糖基转移酶基因在大肠杆菌中表达,将葡萄糖转移到查耳酮的位置2'的活性,即在大肠杆菌的提取液中证实了查耳酮的2'位上的糖基转移酶活性, 证实克隆的基因在2'位编码糖基转移酶。