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    Rose plant named ‘Florirosavio04’
    3.
    植物专利
    Rose plant named ‘Florirosavio04’ 有权
    玫瑰植物名为“Florirosavio04”

    公开(公告)号：USPP23653P2

    公开(公告)日：2013-06-11

    申请号：US12925948

    申请日：2010-11-02

    申请人： Yoshikazu Tanaka ,  Yukihisa Katsumoto ,  Yuko Fukui

    发明人： Yoshikazu Tanaka ,  Yukihisa Katsumoto ,  Yuko Fukui

    IPC分类号： A01H5/00

    CPC分类号： A01H6/749 ,  A01H5/02

    摘要： A new and distinct cultivar of Rose plant named ‘Florirosavio04’, characterized by its upright flowering stems; vigorous growth habit; freely branching plant habit; and strongly fragrant flowers that are light violet in color.

    摘要翻译： 玫瑰植物新品种名称为“Florirosavio04”，其特征是其直立的花茎; 生长习惯旺盛 自由分枝植物习性; 和强烈的芬芳的花朵是浅紫色的颜色。

    Glycosyltransferase gene
    6.
    发明授权
    Glycosyltransferase gene 失效
    糖基转移酶基因

    公开(公告)号：US07514597B2

    公开(公告)日：2009-04-07

    申请号：US10524842

    申请日：2003-08-20

    申请人： Noriko Nakamura ,  Yuko Fukui ,  Eiichiro Ono ,  Yoshikazu Tanaka ,  Hiroaki Okuhara

    发明人： Noriko Nakamura ,  Yuko Fukui ,  Eiichiro Ono ,  Yoshikazu Tanaka ,  Hiroaki Okuhara

    IPC分类号： C12N15/29 ,  C12N15/52 ,  C12N15/82 ,  A01H5/00

    CPC分类号： C12N15/8243 ,  C12N9/1048 ,  C12N15/825

    摘要： The present invention provides an enzyme which catalyzes a reaction to transfer sugar to a hydroxyl group at position 2′ of chalcones and a gene thereof, and preferably an enzyme which catalyzes a reaction to transfer glucose to a hydroxyl group at position 2′ of the chalcones. Furthermore, the invention provides a plant whose flower color has been changed using the glycosyltransferase. Using probes corresponding to conservative regions of the glycosyltransferase, some tens of glycosyltransferase genes having nucleotide sequences corresponding to the conservative regions were cloned from flower petal cDNA libraries of carnation and the like. Furthermore, each of the glycosyltransferase genes was expressed in Escherichia coli, activity to transfer glucose to position 2′ of the chalcone, i.e., the glycosyltransferase activity at position 2′ of chalcone was confirmed in an extract solution of the Escherichia coli, and it was confirmed that cloned genes encoded the glycosyltransferase at position 2′.

    摘要翻译： 本发明提供了一种酶，其催化将糖转移到胡烷酮的2'位的羟基及其基因的反应，优选催化将葡萄糖转移至查尔酮2'位羟基的反应的酶 。 此外，本发明提供了使用糖基转移酶改变花色的植物。 使用与糖基转移酶保守区相对应的探针，从康乃馨花花瓣cDNA文库克隆了数十个具有与保守区相对应的核苷酸序列的糖基转移酶基因。 此外，每个糖基转移酶基因在大肠杆菌中表达，将葡萄糖转移到查耳酮的位置2'的活性，即在大肠杆菌的提取液中证实了查耳酮的2'位上的糖基转移酶活性， 证实克隆的基因在2'位编码糖基转移酶。

    Novel glycosyltransferase gene
    7.
    发明申请
    Novel glycosyltransferase gene 失效
    新型糖基转移酶基因

    公开(公告)号：US20060174377A1

    公开(公告)日：2006-08-03

    申请号：US10524842

    申请日：2003-08-20

    申请人： Noriko Nakamura ,  Yuko Fukui ,  Eichio Ono ,  Yoshikazu Tanaka ,  Hiroaki Okuhara

    发明人： Noriko Nakamura ,  Yuko Fukui ,  Eichio Ono ,  Yoshikazu Tanaka ,  Hiroaki Okuhara

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N9/10 ,  C12N15/82 ,  C12N5/04

    CPC分类号： C12N15/8243 ,  C12N9/1048 ,  C12N15/825

    摘要： The present invention provides an enzyme which catalyzes a reaction to transfer sugar to a hydroxyl group at position 2′ of chalcones and a gene thereof, and preferably an enzyme which catalyzes a reaction to transfer glucose to a hydroxyl group at position 2′ of the chalcones. Furthermore, the invention provides a plant whose flower color has been changed using the glycosyltransferase. Using probes corresponding to conservative regions of the glycosyltransferase, some tens of glycosyltransferase genes having nucleotide sequences corresponding to the conservative regions were cloned from flower petal cDNA libraries of carnation and the like. Furthermore, each of the glycosyltransferase genes was expressed in Escherichia coli, activity to transfer glucose to position 2′ of the chalcone, i.e., the glycosyltransferase activity at position 2′ of chalcone was confirmed in an extract solution of the Escherichia coli, and it was confirmed that cloned genes encoded the glycosyltransferase at position 2′.

    摘要翻译： 本发明提供了一种酶，其催化将糖转移到胡烷酮的2'位的羟基及其基因的反应，优选催化将葡萄糖转移至查尔酮2'位羟基的反应的酶 。 此外，本发明提供了使用糖基转移酶改变花色的植物。 使用与糖基转移酶保守区相对应的探针，从康乃馨花花瓣cDNA文库克隆了数十个具有与保守区相对应的核苷酸序列的糖基转移酶基因。 此外，每个糖基转移酶基因在大肠杆菌中表达，将葡萄糖转移到查耳酮的位置2'的活性，即在大肠杆菌的提取液中证实了查耳酮的2'位上的糖基转移酶活性， 证实克隆的基因在2'位编码糖基转移酶。

    ROSE CONTAINING FLAVONE AND DELPHINIDIN, AND METHOD FOR PRODUCTION THEREOF
    10.
    发明申请
    ROSE CONTAINING FLAVONE AND DELPHINIDIN, AND METHOD FOR PRODUCTION THEREOF 审中-公开
    含有佛罗伦萨和德芬菲的罗辛，及其生产方法

    公开(公告)号：US20100287668A1

    公开(公告)日：2010-11-11

    申请号：US12665051

    申请日：2008-06-19

    申请人： Yoshikazu Tanaka ,  Yukihisa Katsumoto ,  Yuko Fukui ,  Masako Mizutani ,  Noriko Nakamura ,  Junichi Togami

    发明人： Yoshikazu Tanaka ,  Yukihisa Katsumoto ,  Yuko Fukui ,  Masako Mizutani ,  Noriko Nakamura ,  Junichi Togami

    IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/10

    CPC分类号： C12N15/825 ,  C12N9/0071 ,  C12N15/8243

    摘要： The invention provides a rose characterized by comprising a flavone and delphinidin added by a genetic modification method. The flavone and delphinidin are typically produced by expression of a transferred flavone synthase gene and flavonoid 3′,5′-hydroxylase gene, respectively. The flavone synthase gene is, for example, a flavone synthase gene of the family Scrophulariaceae, and specifically it may be the flavone synthase gene of snapdragon of the family Scrophulariaceae, or the flavone synthase gene of torenia of the family Scrophulariaceae. The flavonoid 3′,5′-hydroxylase gene is, for example, the pansy (Viola×wittrockiana) flavonoid 3′,5′-hydroxylase gene.

    摘要翻译： 本发明提供一种玫瑰，其特征在于包含通过遗传修饰方法添加的黄酮和花翠素。 黄酮和花翠素通常分别通过转移的黄酮合成酶基因和类黄酮3'，5'-羟化酶基因的表达产生。 黄酮合成酶基因是例如玄参科的黄酮合成酶基因，特别地，它可以是玄参科的金盏花的黄酮合成酶基因或玄参科的肾上腺素的黄酮合成酶基因。 类黄酮3'，5'-羟化酶基因为例如三色堇（Viola×wittrockiana）黄酮3'，5'-羟化酶基因。