公开(公告)号：US4966784A

公开(公告)日：1990-10-30

申请号：US339015

申请日：1989-04-14

申请人： Mitsutoshi Tanaka ,  Fuminori Arai ,  Kaoru Terashima ,  Nakatsugu Yaginuma

发明人： Mitsutoshi Tanaka ,  Fuminori Arai ,  Kaoru Terashima ,  Nakatsugu Yaginuma

IPC分类号： G01N33/52

CPC分类号： G01N33/526

摘要： A method of preparing an integral multilayer analytical element such as for analysis of calcium comprising a water-impermeable light-transmissive support, a reagent layer containing a water-soluble indicator capable of reacting with an analyte to produce an optically detectable change, and a porous spreading layer containing a spreading action controller and/or an acid capable of decomposing the calcium compounds in a sample, superposed in this order, wherein the spreading action controller and/or the acid is dissolved in an organic solvent which does not dissolve the above water-soluble indicator, this solution is incorporated into the porous spreading layer, and this superposed material is dried. In the method of the present invention, the migration of the water-soluble indicator in the reagent layer at the time of incorporating the spreading action controller or the acid into the spreading layer is inhibited by using a particular organic solvent. Migrations of the indicator and colored material to the spreading layer are also lowered by the spreading action controller, and by these, analytical accuracy of the analytical element is improved. In the case of the analytical element for analysis of calcium, permeation of the acid into other layers than the spreading layer is inhibited, and permeation of a pH buffer in other layer(s) into the spreading layer is also inhibited by using a particular organic solvent. As a result, effective decomposition of calcium compounds in a sample and effective coloration can be achieved.

公开(公告)号：US4871679A

公开(公告)日：1989-10-03

申请号：US73759

申请日：1987-07-15

申请人： Mitsutoshi Tanaka ,  Fuminori Arai ,  Kaoru Terashima ,  Nakatsugu Yaginuma

发明人： Mitsutoshi Tanaka ,  Fuminori Arai ,  Kaoru Terashima ,  Nakatsugu Yaginuma

IPC分类号： G01N33/52

CPC分类号： G01N33/526

摘要： A method of preparing an integral multilayer analytical element such as for analysis of calcium comprising a water-impermeable light-transmissive support, a reagent layer containing a water-soluble indicator capable of reacting with an analyte to produce an optically detectable change, and a porous spreading layer containing a spreading action controller and/or an acid capable of decomposing the calcium compounds in a sample, superposed in this order, wherein the spreading action controller and/or the acid is dissolved in an organic solvent which does not dissolve the above water-soluble indicator, this solution is incorporated into the porous spreading layer, and this superposed material is dried. In the method of the present invention, the migration of the water-soluble indicator in the reagent layer at the time of incorporating the spreading action controller or the acid into the spreading layer is inhibited by using a particular organic solvent. Migrations of the indicator and colored material to the spreading layer are also lowered by the spreading action controller, and by these, analytical accuracy of the analytical element is improved. In the case of the analytical element for analysis of calcium, permeation of the acid into other layers than the spreading layer is inhibited, and permeation of a pH buffer in other layer(s) into the spreading layer is also inhibited by using a particular organic solvent. As a result, effective decomposition of calcium compounds in a sample and effective coloration can be achieved.

公开(公告)号：US4826761A

公开(公告)日：1989-05-02

申请号：US883065

申请日：1986-07-08

申请人： Fuminori Arai ,  Takeshi Igarashi

发明人： Fuminori Arai ,  Takeshi Igarashi

IPC分类号： G01N33/92 ,  C12Q1/00 ,  C12Q1/28 ,  C12Q1/44 ,  C12Q1/60 ,  G01N31/22

CPC分类号： C12Q1/60

摘要： An integral multilayer analytical element for analysis of cholesterol which comprises a water-impermeable light-transmissive support, a hydrophilic layer and a spreading layer superposed in this order, and contains cholesterol esterase, cholesterol oxidase, peroxidase and a coloring reagent composition, which is characterized, in that the above coloring reagent composition is a combination of 4-aminoantipyrine or its derivative and 2-hydroxy-3,5-dichlorobenzene sulfonic acid, and in that pH of the layer where coloring reaction proceeds is kept in the range of 7.5 to 9.This analytical element is stable, and capable of preserving more than 8 months without fogging.

摘要翻译： 用于胆固醇分析的整体多层分析元件，其包含不透水的透光性支持体，亲水层和铺展层，依次叠加，含有胆固醇酯酶，胆固醇氧化酶，过氧化物酶和着色试剂组合物，其特征在于 因为上述着色试剂组合物是4-氨基安替比林或其衍生物与2-羟基-3,5-二氯苯磺酸的组合，并且着色反应进行的层的pH保持在7.5〜 9.这种分析元素是稳定的，能够保存超过8个月而不起雾。

公开(公告)号：US07022285B2

公开(公告)日：2006-04-04

申请号：US09978109

申请日：2001-10-15

申请人： Takaki Arai ,  Fuminori Arai ,  Keiichi Ishizaki

发明人： Takaki Arai ,  Fuminori Arai ,  Keiichi Ishizaki

IPC分类号： G01N31/00

CPC分类号： G01N31/223 ,  Y10T436/175383

摘要： An integral multilayer analytical element for analysis of ammonia or ammonia-producing substance is provided, which comprises a thin liquid blocking layer having a comparative facility with a thicker liquid blocking layer and is stably fabricated on a common production line employed for different type of slides.In the integral multilayer analytical element for analysis of ammonia or ammonia-producing substance comprising a transparent support, an indicator layer containing an indicator which produces a detectable change by gaseous ammonia, a liquid blocking layer permitting a gaseous ammonia to pass through, a reagent layer containing an alkaline buffering agent and optionally a reagent capable of reacting with said ammonia-producing substance to produce ammonia, and a spreading layer, adhesively laminated in this order, the improvement which comprises that said liquid blocking layer is composed of at least two porous membranes.

公开(公告)号：US4939085A

公开(公告)日：1990-07-03

申请号：US107673

申请日：1987-10-09

申请人： Fuminori Arai

发明人： Fuminori Arai

IPC分类号： C12Q1/00 ,  C12Q1/32 ,  G01N31/22 ,  G01N33/52

CPC分类号： G01N33/525 ,  C12Q1/32 ,  Y10S435/805 ,  Y10S436/904

摘要： A dry analytical element having functional layers which contain dehydrogenase, oxidized nicotinamide coenzyme, pyruvate, an electron transport compound and an electron acceptable dye-forming compound is disclosed. The improvement the analytical element resides in that the coenzyme and dye-forming compound are contained in one or two layers which are different from a layer containing pyruvate.

摘要翻译： 公开了具有含有脱氢酶，氧化烟酰胺辅酶，丙酮酸盐，电子传递化合物和电子可接受的染料形成化合物的功能层的干燥分析元件。 分析元素的改进在于辅酶和染料形成化合物包含在不同于含有丙酮酸的层的一层或两层中。

公开(公告)号：US4919890A

公开(公告)日：1990-04-24

申请号：US177287

申请日：1988-04-04

申请人： Fuminori Arai ,  Fusae Osada

发明人： Fuminori Arai ,  Fusae Osada

IPC分类号： G01N33/52

CPC分类号： G01N33/525 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S436/81 ,  Y10T436/206664

摘要： A dry type analysis element for detecting a specific constituent in a liquid is provided. The dry type analysis element comprises a light-transmissive support having thereon at least one water-penetrative layer, at least one of said water-penetrative layer(s) containing a composition capable of interacting with said specific constituent in said liquid, wherein said composition contains a leuco dye comprising at least one compound selected from the group consisting of compounds represented formula [I] and salts of these compounds ##STR1## wherein R.sup.1 represents a substituted or unsubstituted aryl group, R.sup.2 represents a substituted or unsubustituted alkyl group, and R.sup.3 represents a substituted or unsubstituted aryl group,and said leuco dye is dissolved in a hydrophobic solvent to form a solution that is contained in said water-penetrative layer as a dispersion in a hydrophilic medium.

公开(公告)号：US4855109A

公开(公告)日：1989-08-08

申请号：US12693

申请日：1987-02-09

申请人： Katsuaki Muraishi ,  Nakatsugu Yaginuma ,  Kikuo Hirai ,  Fuminori Arai ,  Yuzo Iwata

发明人： Katsuaki Muraishi ,  Nakatsugu Yaginuma ,  Kikuo Hirai ,  Fuminori Arai ,  Yuzo Iwata

IPC分类号： G01N35/00

CPC分类号： G01N35/00029 ,  G01N2035/00039 ,  G01N2035/00089 ,  G01N2035/00356 ,  G01N2035/00752 ,  Y10T436/11 ,  Y10T436/112499

摘要： A chemical analysis apparatus comprises a slide loading section for holding chemical analysis slides, a section for applying a sample material to the chemical analysis slide, and an incubator provided with housing compartments for housing the chemical analysis slides side by side in a transverse direction on the same plane. The apparatus also comprises a read-out head slideable in the transverse direction and disposed to face the chemical analysis slide housed in the housing compartment via a readout opening formed in each housing compartment, thereby emitting light to the chemical analysis slide and measuring the reflection optical density thereof. A conveyance and feed-in system is disposed for conveying the chemical analysis slide held at the slide loading section to the sample applying section, conveying the chemical analysis slide having a reagent layer with the sample material applied thereon to a position facing an inlet opening of the housing compartment, and then feeding the chemical analysis slide into the housing compartment from the inlet opening thereof.

摘要翻译： 化学分析装置包括用于保持化学分析载玻片的载玻片装载部分，将样品材料施加到化学分析载玻片的部分，以及设置有用于容纳化学分析的壳体隔室的培养箱，用于沿横向方向并排滑动 同一架飞机。 该装置还包括可沿横向滑动的读出头，其经由形成在每个容纳室中的读出开口面向容纳在容纳室中的化学分析滑块，从而向化学分析载玻片发射光并测量反射光学 密度。 输送和进料系统被设置用于将保持在滑动装载部分的化学分析载玻片输送到样品施加部分，将具有试剂层的化学分析载玻片输送到其上施加的样品材料到面向入口开口的位置 壳体隔室，然后将化学分析滑块从其入口开口进入壳体隔室。