公开(公告)号：US5047351A

公开(公告)日：1991-09-10

申请号：US351315

申请日：1989-05-10

申请人： Hajime Makiuchi ,  Yuzo Iwata ,  Kikuo Hirai ,  Kenji Murabayashi

发明人： Hajime Makiuchi ,  Yuzo Iwata ,  Kikuo Hirai ,  Kenji Murabayashi

IPC分类号： C12Q1/00 ,  G01N21/27 ,  G01N21/75 ,  G01N21/78 ,  G01N31/22 ,  G01N33/52

CPC分类号： C12Q1/00 ,  G01N21/272 ,  G01N33/521 ,  G01N2021/174 ,  G01N21/78

摘要： A method for shortening the analysis time, without adversely affecting the accuracy in the colorimetric analysis of an analyte. The method including the steps of:(a) Measuring the optical density for two or more times at suitable time intervals after the beginning of reaction to follow the variation of optical density,(b) Judging based upon the optical density obtained at the earlier measurement whether the reaction is to be further continued or not,(c) When it is judged that the continuation of the reaction is not necessary in terms of analytical accuracy, selecting the calibration curve corresponding to the reaction time,(d) While, when it is judged that the continuation of the reaction is necessary, further continuing the reaction, and(e) Repeating steps (b) and (d) until the judgement that the continuation of the reaction is not necessary is obtained.

摘要翻译： 用于缩短分析时间的方法，而不会不利地影响分析物的比色分析的准确性。 该方法包括以下步骤：（a）在反应开始后以合适的时间间隔测量光密度两次或更多次，以遵循光密度的变化，（b）基于在较早测量时获得的光密度 反应是否进一步持续，（c）当判断在分析精度方面不需要继续反应时，选择与反应时间对应的校准曲线，（d）当 判断反应的持续是必要的，进一步继续反应，（e）重复步骤（b）和（d），直到判断出不需要反应的延续为止。

公开(公告)号：US4855109A

公开(公告)日：1989-08-08

申请号：US12693

申请日：1987-02-09

申请人： Katsuaki Muraishi ,  Nakatsugu Yaginuma ,  Kikuo Hirai ,  Fuminori Arai ,  Yuzo Iwata

发明人： Katsuaki Muraishi ,  Nakatsugu Yaginuma ,  Kikuo Hirai ,  Fuminori Arai ,  Yuzo Iwata

IPC分类号： G01N35/00

CPC分类号： G01N35/00029 ,  G01N2035/00039 ,  G01N2035/00089 ,  G01N2035/00356 ,  G01N2035/00752 ,  Y10T436/11 ,  Y10T436/112499

摘要： A chemical analysis apparatus comprises a slide loading section for holding chemical analysis slides, a section for applying a sample material to the chemical analysis slide, and an incubator provided with housing compartments for housing the chemical analysis slides side by side in a transverse direction on the same plane. The apparatus also comprises a read-out head slideable in the transverse direction and disposed to face the chemical analysis slide housed in the housing compartment via a readout opening formed in each housing compartment, thereby emitting light to the chemical analysis slide and measuring the reflection optical density thereof. A conveyance and feed-in system is disposed for conveying the chemical analysis slide held at the slide loading section to the sample applying section, conveying the chemical analysis slide having a reagent layer with the sample material applied thereon to a position facing an inlet opening of the housing compartment, and then feeding the chemical analysis slide into the housing compartment from the inlet opening thereof.

摘要翻译： 化学分析装置包括用于保持化学分析载玻片的载玻片装载部分，将样品材料施加到化学分析载玻片的部分，以及设置有用于容纳化学分析的壳体隔室的培养箱，用于沿横向方向并排滑动 同一架飞机。 该装置还包括可沿横向滑动的读出头，其经由形成在每个容纳室中的读出开口面向容纳在容纳室中的化学分析滑块，从而向化学分析载玻片发射光并测量反射光学 密度。 输送和进料系统被设置用于将保持在滑动装载部分的化学分析载玻片输送到样品施加部分，将具有试剂层的化学分析载玻片输送到其上施加的样品材料到面向入口开口的位置 壳体隔室，然后将化学分析滑块从其入口开口进入壳体隔室。

公开(公告)号：US5948368A

公开(公告)日：1999-09-07

申请号：US947658

申请日：1997-10-09

申请人： Kikuo Hirai ,  Yoshihiko Makino

发明人： Kikuo Hirai ,  Yoshihiko Makino

IPC分类号： G01N33/52 ,  G01N33/48 ,  G01N33/53 ,  G01N33/96 ,  G01N35/00

CPC分类号： G01N33/96 ,  G01N33/48 ,  Y10S435/967

摘要： One sigmoid calibration curve is split into three parts of low concentration region represented by a high degree function, intermediate concentration region represented by an exponential function and high concentration region represented by a high degree function according to the present invention. The boundary condition of the adjacent two functions is set so that the two functions have an equal slope at the boundary point; thereby, regression functions of the calibration curves in respective regions are found. The number of standard samples for finding a calibration curve can be reduced while the calibration curve found is of high accuracy.

摘要翻译： 一个S形校准曲线被分成由高度函数表示的低浓度区域三个部分，由指数函数表示的中间浓度区域和根据本发明的高度函数表示的高浓度区域。 相邻两个函数的边界条件被设置为使得两个函数在边界点具有相等的斜率; 从而发现各个区域的校准曲线的回归函数。 可以减少用于找到校准曲线的标准样品的数量，同时发现校准曲线具有高精度。

公开(公告)号：US5882935A

公开(公告)日：1999-03-16

申请号：US544133

申请日：1995-10-17

申请人： Kikuo Hirai ,  Hiroshi Shinoki ,  Masashi Ogawa ,  Yoshihiko Makino

发明人： Kikuo Hirai ,  Hiroshi Shinoki ,  Masashi Ogawa ,  Yoshihiko Makino

IPC分类号： G01N33/53 ,  G01N33/52 ,  G01N33/542 ,  G01N33/72 ,  H01L21/302 ,  H01L21/304 ,  H01L21/306 ,  H01L21/76

CPC分类号： G01N33/723 ,  G01N33/721

摘要： An analysis element for analyzing both amounts of glycated hemoglobin and total hemoglobin in an aqueous liquid sample to determine glycated hemoglobin content ratio in the sample. The element comprises a substrate layer for receiving a reaction mixture after the completion of an immunological reaction between the glycated hemoglobin in the sample and an enzyme-labelled antibody against the glycated hemoglobin, and a reagent layer. The substrate layer contains a non-diffusible substrate which forms a diffusible material in the presence of the enzyme of the enzyme-labelled antibody, the activity of the enzyme being effected relative to the steric hindrance due to the immunological reaction. The reagent layer contains a reagent composition for reacting with the diffusible material to form a dye detectable colorimetrically in a wavelength range which is not effected by an absorption spectrum of the hemoglobin. The total hemoglobin retained in the substrate layer and the dye formed in the reagent layer are colorimetrically analyzed separately, and the glycated hemoglobin content ratio is calculated from respective values. An analysis method using this analysis element is also provided.

摘要翻译： 分析元素，用于分析含水液体样品中糖化血红蛋白和总血红蛋白的量，以测定样品中糖化血红蛋白含量的比例。 该元件包括用于在样品中的糖化血红蛋白和糖化血红蛋白的酶标记抗体完成后的反应混合物和试剂层的基底层。 底物层含有在酶标记抗体的酶存在下形成可扩散材料的非扩散性底物，该酶的活性相对于由于免疫反应引起的空间位阻影响。 试剂层含有用于与可扩散材料反应的试剂组合物，以形成可在不受血红蛋白的吸收光谱影响的波长范围内比色测定的染料。 分别保留在基材层中的总血红蛋白和形成在试剂层中的染料，并根据各值计算糖化血红蛋白含量比。 还提供了使用该分析元素的分析方法。

公开(公告)号：US4832488A

公开(公告)日：1989-05-23

申请号：US91886

申请日：1987-09-01

申请人： Kikuo Hirai ,  Fuminori Arai ,  Yuzo Iwata

发明人： Kikuo Hirai ,  Fuminori Arai ,  Yuzo Iwata

IPC分类号： G01N21/78 ,  G01N33/52

CPC分类号： G01N33/52 ,  Y10S436/805

摘要： A method for correction of calibration curve in a dry analytical process for quantitative analysis of analyte in a liquid sample such as blood is disclosed. In the method, a calibration curve which has been previously determined for analysis of the analyte using a standard dry analytical element is corrected for application to a dry analytical element having deviated from the standard dry analytical element in its sensitivity to the analyte due to unintentional denaturation, variation, etc., utilizing an optical density determined on the above deviated dry analytical element which has not received spotting of a liquid sample containing the analyte.

公开(公告)号：US5795791A

公开(公告)日：1998-08-18

申请号：US780286

申请日：1997-01-08

申请人： Kikuo Hirai ,  Yoshihiko Makino

发明人： Kikuo Hirai ,  Yoshihiko Makino

IPC分类号： G01N33/52 ,  G01N33/48 ,  G01N33/53 ,  G01N33/96 ,  G01N35/00 ,  G01N33/566

CPC分类号： G01N33/96 ,  G01N33/48 ,  Y10S435/967

摘要： One sigmoid calibration curve is split into three parts of low concentration region represented by a high degree function, intermediate concentration region represented by an exponential function and high concentration region represented by a high degree function according to the present invention. The boundary condition of the adjacent two functions is set so that the two functions have an equal slope at the boundary point; thereby, regression functions of the calibration curves in respective regions are found. The number of standard samples for finding a calibration curve can be reduced while the calibration curve found is of high accuracy.