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公开(公告)号：US5728528A
公开(公告)日：1998-03-17
申请号：US531132
申请日：1995-09-20
CPC分类号： C07H21/00 , C12Q1/6818 , Y10S435/81
摘要： Fluorescent labels are provided employing energy absorber/donor components and energy acceptor/fluorescer components joined together by a spacer which comprises sugar phosphate monomeric units, particularly ribosyl phosphate monomeric units, where the sugar groups are free of active hydroxyl groups. Particularly, an energy transfer component is substituted at the 5' position of the spacer chain, while the other energy transfer component is substituted at the 1' position of the 3' terminal ribosyl group of the label forming an ET cassette for linking to a nucleic acid sequence with any compositions. By employing combinations of ET components, with a common energy absorber/donor and different fluorescers, one can provide for families of labels which can be tagged to any target molecules and which can be excited at a single wavelength and fluoresce at different wavelengths with large Stokes shifts. The compositions find particular application in sequencing.
摘要翻译： 提供使用能量吸收剂/供体组分和能量受体/荧光剂组分的荧光标记，其通过间隔物连接在一起，所述间隔物包含糖磷酸酯单体单元，特别是磷酸核糖基单体单元，其中糖基团不含活性羟基。 特别地，能量转移组分在间隔链的5'位被取代，而另一能量转移组分在形成用于连接到核酸的ET盒的标记的3'端核糖基的1'位置被取代 酸序列与任何组合物。 通过使用ET组分与共同的能量吸收剂/供体和不同的荧光剂的组合，可以提供可以标记任何靶分子的标记系列，并且可以在单个波长处被激发并且在具有大的Stokes的不同波长下发出荧光 转变。 该组合物在测序中发现具体应用。
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公开(公告)号：US5055556A
公开(公告)日：1991-10-08
申请号：US791248
申请日：1985-10-25
申请人： Lubert Stryer , Alexander N. Glazer , Vernon T. Oi
发明人： Lubert Stryer , Alexander N. Glazer , Vernon T. Oi
IPC分类号： G01N33/533
CPC分类号： G01N33/533 , Y10S436/80 , Y10S436/805
摘要： Sensitive detection techniques and compositions for such techniques are provided by employing fluorescent proteins having bilin prosthetic groups as labels. The bilin containing proteins can be conjugated to ligands or receptors for use in systems involving ligand-receptor binding for the analysis, detection or separation of ligands and receptors. Particularly, one or more of the bilin containing proteins may be used as labels in conjunction with each other or other fluorescers for defining subsets of naturally occurring aggregations e.g. cells.
摘要翻译： 通过使用具有胆汁假体基团的荧光蛋白作为标记来提供这种技术的敏感检测技术和组合物。 含有蛋白质的蛋白质可以与配体或受体结合，用于涉及配体 - 受体结合的系统，用于配体和受体的分析，检测或分离。 特别地，一种或多种含胆红蛋白的蛋白质可以用作彼此结合的标记或用于定义天然存在的聚集子集的其它荧光剂。 细胞。
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公开(公告)号：US4857474A
公开(公告)日：1989-08-15
申请号：US717634
申请日：1985-03-29
IPC分类号： C07K14/195 , C07K19/00 , G01N33/533
CPC分类号： G01N33/533 , C07K14/195 , C07K19/00 , Y10S435/822 , Y10S436/80
摘要： This invention provides a class of phycoerythrins useful in diagnostic and detection protocols wherein a fluorescent label is required. The unique spectral properties of the phycoerythrins described herein provide for increased sensitivity and alternative uses in assays employing them.
摘要翻译： 本发明提供一类可用于诊断和检测方案的藻红蛋白，其中需要荧光标记。 本文所述的藻红蛋白的独特的光谱性质提供增加的敏感性和使用它们的测定中的替代用途。
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34.发明授权Fluorescent immunoassay employing a phycobiliprotein labeled ligand or receptor 失效使用藻胆蛋白标记的配体或受体进行荧光免疫测定
公开(公告)号：US4520110A
公开(公告)日：1985-05-28
申请号：US454768
申请日：1982-12-30
申请人： Lubert Stryer , Alexander N. Glazer , Vernon T. Oi
发明人： Lubert Stryer , Alexander N. Glazer , Vernon T. Oi
IPC分类号： G01N33/533 , G01N33/54 , G01N31/00
CPC分类号： G01N33/533 , Y10S436/80 , Y10S530/825
摘要： Sensitive detection techniques and compositions for such techniques employing fluorescent proteins having bilin prosthetic groups as labels i.e. phycobiliprotein. The bilin containing proteins can be conjugated to ligands or receptors for use in systems involving ligand-receptor binding for the analysis, detection or separation of ligands and receptors. Particularly, one or more of the bilin containing proteins may be used as labels in conjuction with each other or other fluorescers for defining subsets of naturally occurring aggregations e.g. cells.
摘要翻译： 敏感检测技术和使用具有胆汁假体基团的荧光蛋白质的技术的组合物，即藻胆蛋白。 含有蛋白质的蛋白质可以与配体或受体结合，用于涉及配体 - 受体结合的系统，用于配体和受体的分析，检测或分离。 特别地，一种或多种含胆红蛋白的蛋白质可以用作彼此结合的标记或用于定义天然存在的聚集子集的其它荧光剂。 细胞。
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