中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

44 records (took 0.044 seconds)

    Methods For Producing Multiple Recombinant Polypeptides In A Filamentous Fungal Host Cell
    33.
    发明申请
    Methods For Producing Multiple Recombinant Polypeptides In A Filamentous Fungal Host Cell 审中-公开
    在丝状真菌宿主细胞中产生多种重组多肽的方法

    公开(公告)号：US20170015986A1

    公开(公告)日：2017-01-19

    申请号：US15280706

    申请日：2016-09-29

    Applicant: Novozymes, Inc.

    Inventor： Debbie Yaver ,  Qiming Jin

    IPC: C12N9/42 ,  C12N15/80

    CPC classification number: C12N9/2437 ,  C12N9/2402 ,  C12N15/80 ,  C12N15/87 ,  C12Y302/01091 ,  C12Y302/0115

    Abstract: The present invention relates to methods for constructing a filamentous fungal strain for production of multiple recombinant polypeptides having biological activity. The present invention also relates to methods for producing multiple recombinant polypeptides having biological activity in a filamentous fungal strain. The present invention also relates to filamentous fungal strains expressing multiple recombinant polypeptides having biological activity.

    Abstract translation: 本发明涉及构建用于生产具有生物活性的多重重组多肽的丝状真菌菌株的方法。 本发明还涉及在丝状真菌菌株中生产具有生物活性的多重重组多肽的方法。 本发明还涉及表达具有生物活性的多种重组多肽的丝状真菌菌株。

    Methods for producing heterologous polypeptides in mutants of trichoderma
    34.
    发明授权
    Methods for producing heterologous polypeptides in mutants of trichoderma 有权

    公开(公告)号：US10023872B2

    公开(公告)日：2018-07-17

    申请号：US14652755

    申请日：2013-12-20

    Applicant: Novozymes, Inc.

    Inventor： Sandra Merino ,  Judilee Quintos ,  Debbie Yaver

    IPC: C12N15/00 ,  C12P21/00 ,  C12N15/52 ,  C12P21/02 ,  C12N9/88 ,  C12N9/00

    Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more genes selected from the group consisting of a peptaibol synthetase gene, a paracelsin synthetase gene, a first terpene cyclase gene, a second terpene cyclase gene, and a third terpene cyclase gene, wherein one or more of the genes are modified rendering the mutant strain deficient in the production of one or more of the enzymes selected from the group consisting of a peptaibol synthetase, a paracelsin synthetase, a first terpene cyclase, a second terpene cyclase, and a third terpene cyclase compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

    Promoter variants for expressing genes in a fungal cell
    35.
    发明授权
    Promoter variants for expressing genes in a fungal cell 有权

    公开(公告)号：US09695429B2

    公开(公告)日：2017-07-04

    申请号：US14850448

    申请日：2015-09-10

    Applicant: Novozymes, Inc.

    Inventor： Debbie Yaver ,  Peter Nham

    IPC: C12N15/80 ,  C12P21/00 ,  C12P1/02 ,  C12N15/113

    CPC classification number: C12N15/80 ,  C12N9/0044 ,  C12N9/20 ,  C12N9/58 ,  C12N9/93 ,  C12N15/113 ,  C12P1/02 ,  C12P21/00 ,  C12Y107/01003 ,  C12Y301/01003 ,  C12Y304/21004 ,  C12Y603/04002

    Abstract: The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, and SEQ ID NO: 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.

    Promoter Variants For Expressing Genes In A Fungal Cell
    40.
    发明申请
    Promoter Variants For Expressing Genes In A Fungal Cell 审中-公开
    在真菌细胞中表达基因的启动子变体

    公开(公告)号：US20150376630A1

    公开(公告)日：2015-12-31

    申请号：US14850448

    申请日：2015-09-10

    Applicant: Novozymes, Inc.

    Inventor： Debbie Yaver ,  Peter Nham

    IPC: C12N15/80 ,  C12P21/00

    CPC classification number: C12N15/80 ,  C12N9/0044 ,  C12N9/20 ,  C12N9/58 ,  C12N9/93 ,  C12N15/113 ,  C12P1/02 ,  C12P21/00 ,  C12Y107/01003 ,  C12Y301/01003 ,  C12Y304/21004 ,  C12Y603/04002

    Abstract: The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, and SEQ ID NO: 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.

    Abstract translation: 本发明涉及生物体的制造方法，其特征在于，包括：（a）在有助于生物生物的培养基中培养真菌宿主细胞，其中所述真菌宿主细胞可操作地包含编码生物物质的第一核酸序列 连接到第二核酸序列，其包含选自SEQ ID NO：2，SEQ ID NO：3，SEQ ID NO：4，SEQ ID NO：5，SEQ ID NO：6，SEQ ID NO： ：7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10，SEQ ID NO：11和SEQ ID NO：12; 及其后果; 及其杂交和串联启动子; 和（b）从培养基中分离生物物质。 本发明还涉及分离的启动子变体和构建体，载体和真菌宿主细胞，其包含可操作地连接于编码生物物质的核酸序列的启动子变体。

Patent Agency Ranking