公开(公告)号：US07413911B2

公开(公告)日：2008-08-19

申请号：US11204169

申请日：2005-08-16

申请人： Hisashi Ohtsuka

发明人： Hisashi Ohtsuka

IPC分类号： G01N33/553

CPC分类号： G01N21/553 ,  Y10S436/805

摘要： An object of the present invention is to provide a method for measuring a rate coefficient by surface plasmon resonance analysis with excellent accuracy, reliability, and cost performance that can improve the assay accuracy and simplify the apparatus. The present invention is provides a method for determining the adsorption rate coefficient (Ka) and the dissociation rate coefficient (Kd) of the reaction between an analyte molecule immobilized on a metal surface and a molecule that interacts with the analyte molecule by measuring changes in surface plasmon resonance signals using a surface plasmon resonance measurement device and performing fitting to a signal curve obtained by such measurement, wherein fitting is performed to the signals from which data obtained at the time of liquid exchange and data obtained at a given time point thereafter have been removed, and that an algorithm is adopted wherein the fitted curve passes through a point representing a baseline value obtained at the time of liquid exchange or immediately therebefore.

摘要翻译： 本发明的目的是提供一种通过表面等离子体共振分析测量速率系数的方法，该方法具有极好的精度，可靠性和成本性能，可以提高测定精度并简化装置。 本发明提供了一种通过测量表面变化来确定固定在金属表面上的分析物分子与与分析物分子相互作用的分子之间的反应的吸附速率系数（Ka）和解离速率系数（Kd）的方法 使用表面等离子体共振测量装置进行等离子体共振信号，并且对通过这种测量获得的信号曲线进行拟合，其中对于在其间进行液体交换时获得的数据和在此后给定时间点获得的数据的信号进行拟合 并且采用一种算法，其中拟合曲线通过表示在液体交换时或之前立即获得的基线值的点。

公开(公告)号：US07403554B2

公开(公告)日：2008-07-22

申请号：US10952719

申请日：2004-09-30

申请人： Hisashi Ohtsuka ,  Yoji Okazaki ,  Takayuki Katoh

发明人： Hisashi Ohtsuka ,  Yoji Okazaki ,  Takayuki Katoh

IPC分类号： H01S3/094

CPC分类号： H01S3/067 ,  H01S3/025 ,  H01S3/0405 ,  H01S3/042 ,  H01S3/0602 ,  H01S3/09415 ,  H01S3/109 ,  H01S3/1603 ,  H01S3/161 ,  H01S3/1615 ,  H01S5/32341 ,  H01S2302/00

摘要： A solid-state laser crystal constituting a laser-diode-excited solid-state laser apparatus or an optical fiber constituting a fiber laser apparatus or fiber laser amplifier is doped with one of Ho3+, Sm3+, Eu3+, Dy3+, Er3+, and Tb3+ so that a laser beam is emitted from the solid-state laser crystal or the optical fiber, or incident light of the fiber laser amplifier is amplified, by one of the transitions from 5S2 to 5I7, from 5S2 to 5I8, from 4G5/2 to 6H5/2, from 4G5/2 to 6H7/2, from 4F3/2 to 6H11/2, from 5D0 to 7F2, from 4F9/2 to 6H13/2, from 4F9/2 to 6H11/2, from 4S3/2 to 4I15/2, from 2H9/2 to 4I13/2, and from 5D4 to 7F5. The above solid-state laser-crystal or optical fiber is excited with a GaN-based compound laser diode.

公开(公告)号：US07362789B2

公开(公告)日：2008-04-22

申请号：US10952760

申请日：2004-09-30

申请人： Hisashi Ohtsuka ,  Yoji Okazaki ,  Takayuki Katoh

发明人： Hisashi Ohtsuka ,  Yoji Okazaki ,  Takayuki Katoh

IPC分类号： H01S3/094

CPC分类号： H01S3/067 ,  H01S3/025 ,  H01S3/0405 ,  H01S3/042 ,  H01S3/0602 ,  H01S3/09415 ,  H01S3/109 ,  H01S3/1603 ,  H01S3/161 ,  H01S3/1615 ,  H01S5/32341 ,  H01S2302/00

摘要： A solid-state laser crystal constituting a laser-diode-excited solid-state laser apparatus or an optical fiber constituting a fiber laser apparatus or fiber laser amplifier is doped with one of Ho3+, Sm3+, Eu3+, Dy3+, Er3+, and Tb3+ so that a laser beam is emitted from the solid-state laser crystal or the optical fiber, or incident light of the fiber laser amplifier is amplified, by one of the transitions from 5S2 to 5I7, from 5S2 to 5I8, from 4G5/2 to 6H5/2, from 4G5/2 to 6H7/2, from 4F3/2 to 6H11/2, from 5D0 to 7F2, from 4F9/2 to 6H13/2, from 4F9/2 to 6H11/2, from 4S3/2 to 4I15/2, from 2H9/2 to 4I13/2, and from 5D4 to 7F5. The above solid-state laser crystal or optical fiber is excited with a GaN-based compound laser diode.

公开(公告)号：US07330263B2

公开(公告)日：2008-02-12

申请号：US11049893

申请日：2005-02-04

申请人： Hisashi Ohtsuka

发明人： Hisashi Ohtsuka

IPC分类号： G01N21/55

CPC分类号： B82Y30/00 ,  B82Y15/00 ,  G01N21/553

摘要： A measurement apparatus includes a dielectric block, a laser light source for generating a light beam, an optical system for causing the light beam to enter the interface between the dielectric block and a metal film at various incident angles, and a light detection unit for detecting a parallelized light beam totally reflected at the interface. Properties are measured while causing a reference molecule, of substantially the same size and the same refractive index as an analysis object molecule, to adhere onto the metal film. The reference molecule is removed from the metal film, and the position of a dark line is measured while the measurement chip retains a sample. The position of a dark line of the analysis object molecule is detected by calibrating sensitivity based on the detection result of the position of the dark line while causing the reference molecule to adhere onto the metal film.

摘要翻译： 测量装置包括介质块，用于产生光束的激光源，用于使光束以各种入射角进入介质块和金属膜之间的界面的光学系统，以及用于检测的光检测单元 在界面处完全反射的平行光束。 测量属性，同时引起与分析对象分子基本相同的尺寸和相同折射率的参考分子附着在金属膜上。 将参考分子从金属膜上除去，并且在测量芯片保留样品的同时测量暗线的位置。 通过基于黑线的位置的检测结果，同时使参考分子附着在金属膜上，通过校准灵敏度来检测分析对象分子的暗线的位置。

公开(公告)号：US20070158549A1

公开(公告)日：2007-07-12

申请号：US11642535

申请日：2006-12-21

申请人： Masayuki Naya ,  Hisashi Ohtsuka

发明人： Masayuki Naya ,  Hisashi Ohtsuka

IPC分类号： H01J49/00

CPC分类号： H01J49/0418 ,  G01N21/554 ,  G01N21/658

摘要： A base plate for mass spectrometry analysis is disclosed, which is used in a method in which a substance immobilized on a surface of the base plate is desorbed from the surface by application of laser light thereto and the ion of the desorbed substance is captured for mass spectrometry analysis. The base plate includes, on at least a portion of the surface thereof, a roughened metal surface capable of exciting local plasmon when exposed to laser light. The roughened metal surface is formed, for example, by forming numerous micropores in a surface of an alumina layer and filling gold particles in the micropores. Each gold particle has a head portion having a size larger than a diameter of the micropore and projecting from the surface of the alumina layer. Use of this base plate allows use of lower-power laser light.

摘要翻译： 公开了一种用于质谱分析的基板，其用于通过施加激光将固定在基板表面上的物质从表面解吸并且解吸附物质的离子被捕获的方法 光谱分析。 基板在其表面的至少一部分上包括当暴露于激光时能够激发局部等离子体激元的粗糙金属表面。 粗糙金属表面例如通过在氧化铝层的表面中形成许多微孔并在微孔中填充金颗粒而形成。 每个金颗粒具有头部，其尺寸大于微孔的直径并从氧化铝层的表面突出。 使用该底板允许使用较低功率的激光。

公开(公告)号：US07154930B2

公开(公告)日：2006-12-26

申请号：US10952768

申请日：2004-09-30

申请人： Hisashi Ohtsuka ,  Yoji Okazaki ,  Takayuki Katoh

发明人： Hisashi Ohtsuka ,  Yoji Okazaki ,  Takayuki Katoh

IPC分类号： H01S3/30 ,  H01S3/091 ,  H01S3/094

CPC分类号： H01S3/067 ,  H01S3/025 ,  H01S3/0405 ,  H01S3/042 ,  H01S3/0602 ,  H01S3/09415 ,  H01S3/109 ,  H01S3/1603 ,  H01S3/161 ,  H01S3/1615 ,  H01S5/32341 ,  H01S2302/00

摘要： A solid-state laser crystal constituting a laser-diode-excited solid-state laser apparatus or an optical fiber constituting a fiber laser apparatus or fiber laser amplifier is doped with one of Ho3+, Sm3+, Eu3+, Dy3+, Er3+, and Tb3+ so that a laser beam is emitted from the solid-state laser crystal or the optical fiber, or incident light of the fiber laser amplifier is amplified, by one of the transitions from 5S2 to 5I7, from 5S2 to 5I8, from 4G5/2 to 6H5/2, from 4G5/2 to 6H7/2, from 4F3/2 to 6H11/2, from 5D0 to 7F2, from 4F9/2 to 6H13/2, from 4F9/2 to 6H11/2, from 4S3/2 to 4I15/2, from 2H9/2 to 4I13/2, and from 5D4 to 7F5. The above solid-state laser crystal or optical fiber is excited with a GaN-based compound laser diode.

公开(公告)号：US20060040409A1

公开(公告)日：2006-02-23

申请号：US11204006

申请日：2005-08-16

申请人： Hisashi Ohtsuka ,  Nobuhiko Ogura

发明人： Hisashi Ohtsuka ,  Nobuhiko Ogura

IPC分类号： G06F19/00 ,  G01N33/551

CPC分类号： G01N33/54373 ,  G01N21/272 ,  G01N21/553 ,  G01N33/542 ,  Y10S436/805

摘要： An object of the present invention is to provide a method for measuring the dissociation rate coefficient (Kd) by surface plasmon resonance analysis without measuring the theoretical maximum amount of binding (Rmax). The present invention provides a method for measuring the dissociation rate coefficient (Kd) of the reaction between an analyte molecule immobilized on a metal surface and a molecule that interacts with the analyte molecule by assaying changes in surface plasmon resonance signals using a surface plasmon resonance measurement device, wherein the signal and the slope of the dissociation curve of the surface plasmon resonance signal curves, or the signal ratio are used to calculate the dissociation rate coefficient (Kd).

摘要翻译： 本发明的目的是提供一种通过表面等离子体共振分析测量解离速率系数（Kd）而不测量理论最大结合量（Rmax）的方法。 本发明提供了一种用于测量固定在金属表面上的分析物分子与通过使用表面等离子体共振测量法测定表面等离子体共振信号的变化而与分析物分子相互作用的分子之间的反应的解离速率系数（Kd）的方法 装置，其中表面等离子体共振信号的解离曲线的信号和斜率曲线或信号比用于计算解离速率系数（Kd）。

公开(公告)号：US06816532B2

公开(公告)日：2004-11-09

申请号：US10143806

申请日：2002-05-14

申请人： Hisashi Ohtsuka ,  Yoji Okazaki ,  Takayuki Katoh

发明人： Hisashi Ohtsuka ,  Yoji Okazaki ,  Takayuki Katoh

IPC分类号： H01S3094

CPC分类号： H01S3/067 ,  H01S3/025 ,  H01S3/0405 ,  H01S3/042 ,  H01S3/0602 ,  H01S3/09415 ,  H01S3/109 ,  H01S3/1603 ,  H01S3/161 ,  H01S3/1615 ,  H01S5/32341 ,  H01S2302/00

摘要： A solid-state laser crystal constituting a laser-diode-excited solid-state laser apparatus or an optical fiber constituting a fiber laser apparatus or fiber laser amplifier is doped with one of Ho3+, Sm3+, Eu3+, Dy3+, Er3+, and Tb3+ so that a laser beam is emitted from the solid-state laser crystal or the optical fiber, or incident light of the fiber laser amplifier is amplified, by one of the transitions from 5S2 to 5I7, from 5S2 to 5I8, from 4G5/2 to 6H5/2, from 4G5/2 to 6H7/2, from 4F3/2 to 6H11/2, from 5D0 to 7F2, from 4F9/2 to 6H13/2, from 4F9/2 to 6H11/2, from 4S3/2 to 4I15/2, from 2H9/2 to 4I13/2, and from 5D4 to 7F5. The above solid-state laser crystal or optical fiber is excited with a GaN-based compound laser diode.

摘要翻译： 构成激光二极管激光固体激光装置的固态激光晶体或构成光纤激光装置或光纤激光放大器的光纤掺杂有Ho 3+，Sm 3+， 3+，Dy 3+，Er 3+和Tb 3+，使得激光束从固态激光晶体或光纤发射，或者光纤激光放大器的入射光为 通过从<5> S2至<5> I7的从5S→5J8→4G5 / 2→6H5 / 2→4G5 / 2〜6 H7 / 2，从F3 / 2〜6H11 / 2，从5℃到0℃的F2，从F9 / 2到6的H13 / 2， 从4/3> 3/1> 1/2，从4/3> 911/2到<6> H11 / 2，从S3 / D4至<7> F5。 上述固态激光晶体或光纤被GaN基复合激光二极管激发。

公开(公告)号：US08456158B2

公开(公告)日：2013-06-04

申请号：US12845260

申请日：2010-07-28

申请人： Hisashi Ohtsuka

发明人： Hisashi Ohtsuka

IPC分类号： G01N27/84

CPC分类号： G01N33/54333 ,  B03C1/01 ,  B03C1/28 ,  B03C2201/18 ,  B03C2201/24 ,  B03C2201/26 ,  G01N33/54306

摘要： A magnetic binding substance, which is a first binding substance that specifically binds with a target substance, having magnet enveloping dielectric particles, which have magnetic particles enveloped therein and surfaces modified with functional groups that exhibit polarity within a liquid sample, attached thereto, and a labeling binding substance, which is a second binding substance that specifically binds with the target substance having photoresponsive labels attached thereto, are mixed with the liquid sample such that binding reactions occur. A magnetic field is generated within a sample cell, to draw the magnetic binding substance to a local region. Excitation light is irradiated only onto a predetermined region including the local region while the magnetic binding substance is drawn to the local region, causing the photoresponsive labels present therein to generate optical signals. The optical signals are detected.

摘要翻译： 一种磁性结合物质，其是与靶物质特异性结合的具有磁性包裹电介质粒子的第一结合物质，其中包含磁性粒子，并附着在液体样品中显示极性的官能团修饰的表面，以及 标记结合物质，其是与附着有光响应标记物的目标物质特异性结合的第二结合物质与液体样品混合，从而发生结合反应。 在样品池内产生磁场，将磁性结合物质吸引到局部区域。 激励光仅在包括局部区域的预定区域被照射，同时将磁性物质吸引到局部区域时，使其中存在的光响应标签产生光信号。 检测到光信号。

公开(公告)号：US08039268B2

公开(公告)日：2011-10-18

申请号：US12422587

申请日：2009-04-13

申请人： Hisashi Ohtsuka

发明人： Hisashi Ohtsuka

IPC分类号： G01N33/53

CPC分类号： G01N33/54386 ,  Y10S435/81 ,  Y10S435/975 ,  Y10S436/808

摘要： An immunochromatoassay method that allows high detection sensitivity measurement. The method including the steps of: permeating an analyte solution that includes a visibly labeled second binding substance that specifically binds to a detection target substance into a test area of a chromatography medium provided with a first binding substance that specifically binds to the detection target substance, simultaneously with or after the permeation of the analyte solution into the test area, permeating a visual recognition aid solution into the chromatograph medium, the solution having a refractive index whose refractive index difference Δn from that of the chromatograph medium is −0.1≦Δn≦0.1, and visually observing the test area while the visual recognition aid solution is permeated in the test area.

摘要翻译： 允许高检测灵敏度测量的免疫色谱测定方法。 该方法包括以下步骤：将包含特异性结合检测对象物质的可视标记的第二结合物质的分析物溶液渗入具有特异性结合检测对象物质的第一结合物质的色谱介质的检测区域， 在将分析物溶液渗透到测试区域中或之后，将视觉识别助溶剂渗透到色谱介质中，该溶液具有与色谱介质的折射率差＆Dgr; n的折射率为-0.1＆lt; 1lE; ＆Dgr; n＆nlE; 0.1，并且在视觉识别辅助溶液渗入测试区域时目视观察测试区域。