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101 条记录 (耗时 0.039 秒)

    Bioluminescent caspase assay compounds
    34.
    发明授权
    Bioluminescent caspase assay compounds 有权
    生物发光半胱天冬酶测定化合物

    公开(公告)号：US07666987B2

    公开(公告)日：2010-02-23

    申请号：US11347054

    申请日：2006-02-03

    申请人： Martha O'Brien ,  Keith V. Wood ,  Dieter Klaubert ,  William Daily

    发明人： Martha O'Brien ,  Keith V. Wood ,  Dieter Klaubert ,  William Daily

    IPC分类号： C07K1/00

    CPC分类号： C07D277/66 ,  C12Q1/37 ,  C12Q1/66

    摘要： A sensitive bioluminescent assay to detect proteases including caspases is provided which employs an aminoluciferin or a carboxy-terminal protected derivative thereof covalently linked via a peptide bond to a substrate for a caspase or an aminoluciferin or a carboxy-terminal protected derivative thereof covalently linked via a peptide bond to a peptide substrate comprising aspartate that is specifically cleaved by a protease specific for the substrate.

    摘要翻译： 提供了用于检测包括胱天蛋白酶的蛋白酶的敏感生物发光测定法，其使用通过肽键共价连接的氨基荧光素或其羧基端保护的衍生物与底物进行共价连接的胱天蛋白酶或氨基荧光素或羧基端保护的衍生物 肽结合到包含由对底物特异的蛋白酶特异性切割的天冬氨酸的肽底物。

    Bioluminescent protease assay with modified aminoluciferin or derivatives thereof
    37.
    发明授权
    Bioluminescent protease assay with modified aminoluciferin or derivatives thereof 有权
    具有修饰的氨基荧光素或其衍生物的生物发光蛋白酶测定

    公开(公告)号：US07384758B2

    公开(公告)日：2008-06-10

    申请号：US11346043

    申请日：2006-02-02

    申请人： Martha O'Brien ,  Keith V. Wood ,  Dieter Klaubert ,  William Daily

    发明人： Martha O'Brien ,  Keith V. Wood ,  Dieter Klaubert ,  William Daily

    IPC分类号： C12Q1/66

    CPC分类号： C07D277/66 ,  C12Q1/37 ,  C12Q1/66

    摘要： A sensitive bioluminescent assay to detect proteases, including caspases and proteases that specifically cleave a peptide substrate having aspartate, is provided. In one embodiment, the assay employs an aminoluciferin or a carboxy-terminal protected derivative thereof covalently linked via a peptide bond to a substrate for a caspase or an aminoluciferin or a carboxy-terminal protected derivative thereof covalently linked via a peptide bond to a peptide substrate comprising aspartate that is specifically cleaved by a protease specific for the substrate.

    摘要翻译： 提供了一种敏感的生物发光测定法，用于检测蛋白酶，包括特异性切割具有天冬氨酸的肽底物的胱天蛋白酶和蛋白酶。 在一个实施方案中，该测定使用通过肽键共价连接的氨基荧光素或其羧基末端保护的衍生物与底物共价连接到通过肽键与肽底物共价连接的胱天蛋白酶或氨基荧光素或其羧基端保护的衍生物 其包含特异性用于底物的蛋白酶特异性切割的天冬氨酸。

    Method for increasing luminescence assay sensitivity
    40.
    发明授权
    Method for increasing luminescence assay sensitivity 有权
    增加发光测定灵敏度的方法

    公开(公告)号：US07118878B1

    公开(公告)日：2006-10-10

    申请号：US09590884

    申请日：2000-06-09

    申请人： Erika Hawkins ,  John M. Centanni ,  Jacqueline Sankbeil ,  Keith V. Wood

    发明人： Erika Hawkins ,  John M. Centanni ,  Jacqueline Sankbeil ,  Keith V. Wood

    IPC分类号： C12Q1/66

    CPC分类号： C12Q1/66 ,  G01N33/582 ,  G01N2333/90241 ,  Y10S435/968 ,  Y10S435/975

    摘要： The invention provides kits and methods for increasing the sensitivity of a bio-luminescent assay, which employ an organic compound that, for instance, reduces luminescence that is not dependent on the presence of an analyte by at least about 10 fold and reduces luminescence that is dependent on the presence of an analyte by less than about 7 fold, reduces luminescence generated by luminogenic molecules not bound to an enzyme by at least about 10 fold and reduces the luminescence generated by luminogenic molecules bound to an enzyme by less than about 7 fold, or reduces autoluminescence by at least about 10 fold and reduces luminescence that is dependent on the presence of an analyte by less than about 7 fold.

    摘要翻译： 本发明提供用于提高生物发光测定的灵敏度的试剂盒和方法，其使用有机化合物，例如将不依赖于分析物的发光的发光减少至少约10倍，并且减少发光， 取决于分析物的存在小于约7倍，减少不与酶结合至少约10倍的发光分子产生的发光，并将由酶结合的发光分子产生的发光降低小于约7倍， 或将自发光发光降低至少约10倍，并且减少依赖于分析物的存在小于约7倍的发光。