公开(公告)号：US09816054B2

公开(公告)日：2017-11-14

申请号：US12249584

申请日：2008-10-10

申请人： Sergei Saveliev ,  Daniel Simpson ,  Keith V. Wood ,  Harry Tetsuo Uyeda ,  Carolyn Woodroofe Hitko ,  Dieter Klaubert

发明人： Sergei Saveliev ,  Daniel Simpson ,  Keith V. Wood ,  Harry Tetsuo Uyeda ,  Carolyn Woodroofe Hitko ,  Dieter Klaubert

IPC分类号： C07C309/64 ,  C11D1/20 ,  C07C309/10 ,  C07C309/14 ,  C07C309/15 ,  C07C309/16 ,  C07C323/12 ,  C07C329/06 ,  C07D307/40 ,  C07D307/56 ,  C07D317/22 ,  C07D333/16 ,  C11D1/14

CPC分类号： C11D1/20 ,  C07C309/10 ,  C07C309/14 ,  C07C309/15 ,  C07C309/16 ,  C07C323/12 ,  C07C329/06 ,  C07D307/40 ,  C07D307/56 ,  C07D317/22 ,  C07D333/16 ,  C11D1/143

摘要： The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.

公开(公告)号：US20090095628A1

公开(公告)日：2009-04-16

申请号：US12249584

申请日：2008-10-10

申请人： Sergei Saveliev ,  Daniel Simpson ,  Keith V. Wood

发明人： Sergei Saveliev ,  Daniel Simpson ,  Keith V. Wood

IPC分类号： C07K1/26 ,  C07C309/64 ,  C07D307/02 ,  C07D317/10 ,  C07K1/14 ,  C12N9/96 ,  C12Q1/37 ,  C07K1/16

CPC分类号： C11D1/20 ,  C07C309/10 ,  C07C309/14 ,  C07C309/15 ,  C07C309/16 ,  C07C323/12 ,  C07C329/06 ,  C07D307/40 ,  C07D307/56 ,  C07D317/22 ,  C07D333/16 ,  C11D1/143

摘要： The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.

摘要翻译： 本发明提供式I-IX的表面活性剂化合物，其可用于帮助生物材料例如蛋白质或细胞膜的增溶，消化，制备，分析和/或表征的方法。 这些化合物还可以帮助回收在蛋白质消化过程中产生的肽，特别是对于凝胶内消化方案。 另外，这些化合物可以改善酶蛋白去糖基化而不干扰下游样品制备步骤和质谱分析。 这些化合物可以特别用作能够被酸，加热或其组合分解的消化助剂。 表面活性剂的分解允许从分离的样品中容易地分离，和/或允许分析样品而不干扰各种分析技术的灵敏度。

公开(公告)号：US08921620B2

公开(公告)日：2014-12-30

申请号：US13450217

申请日：2012-04-18

申请人： Keith V. Wood ,  Dieter Klaubert ,  Georgyi V. Los ,  Robert F. Bulleit ,  Mark McDougall ,  Chad Zimprich

发明人： Keith V. Wood ,  Dieter Klaubert ,  Georgyi V. Los ,  Robert F. Bulleit ,  Mark McDougall ,  Chad Zimprich

IPC分类号： C07C19/00 ,  C07C21/00 ,  G01N33/53 ,  C12Q1/34 ,  C12N9/14

CPC分类号： C12Q1/34 ,  C07C217/08 ,  C07C271/16 ,  C07D209/48 ,  C07D233/64 ,  C07D311/78 ,  C07D311/82 ,  C07D405/06 ,  C07D493/10 ,  C07D495/04 ,  C07F5/022 ,  C12N9/14 ,  C12N9/86 ,  C12Y308/01005 ,  G01N33/5005 ,  G01N33/58 ,  Y10S436/80

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

公开(公告)号：US08476450B2

公开(公告)日：2013-07-02

申请号：US12217494

申请日：2008-07-03

申请人： James J. Cali ,  William Daily ,  Erika Hawkins ,  Dieter Klaubert ,  Jianquan Liu ,  Poncho Meisenheimer ,  Michael Scurria ,  John Shultz ,  James Unch ,  Michael P. Valley ,  Keith V. Wood ,  Wenhui Zhou

发明人： James J. Cali ,  William Daily ,  Erika Hawkins ,  Dieter Klaubert ,  Jianquan Liu ,  Poncho Meisenheimer ,  Michael Scurria ,  John Shultz ,  James Unch ,  Michael P. Valley ,  Keith V. Wood ,  Wenhui Zhou

IPC分类号： A61K31/428 ,  C07D417/04 ,  C07D417/14

CPC分类号： C07D277/66 ,  C07D277/68 ,  C07D311/16 ,  C07D417/04 ,  C07D417/14 ,  C07D493/10 ,  C07F9/65583 ,  C09B11/24 ,  C09B19/00 ,  C09B57/00 ,  C09B57/02 ,  C12Q1/66 ,  G01N2500/00

摘要： A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.

摘要翻译： 提供了检测采用荧光素衍生物或荧光团衍生物的样品中至少一种分子的存在或量的方法。 还提供了用于实施本发明方法的化合物和组合物。

公开(公告)号：US20120330001A1

公开(公告)日：2012-12-27

申请号：US13477593

申请日：2012-05-22

申请人： Aldis Darzins ,  Lance Encell ,  Tonny Johnson ,  Dieter Klaubert ,  Georgyi V. Los ,  Mark McDougall ,  Keith V. Wood ,  Monika G. Wood ,  Chad Zimprich

发明人： Aldis Darzins ,  Lance Encell ,  Tonny Johnson ,  Dieter Klaubert ,  Georgyi V. Los ,  Mark McDougall ,  Keith V. Wood ,  Monika G. Wood ,  Chad Zimprich

IPC分类号： C07C235/08 ,  C07C19/075 ,  C08B1/00 ,  C07C19/01

CPC分类号： C12N9/14 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  C07K2299/00 ,  C12N9/0069 ,  C12Q1/26 ,  C12Q1/34 ,  C12Q1/66 ,  C12Y113/12005 ,  C12Y308/01005

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代 野生型水解酶。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

公开(公告)号：US08257939B2

公开(公告)日：2012-09-04

申请号：US12975020

申请日：2010-12-21

申请人： Keith V. Wood ,  Georgyi V. Los ,  Robert F. Bulleit ,  Dieter Klaubert ,  Mark McDougall ,  Chad Zimprich

发明人： Keith V. Wood ,  Georgyi V. Los ,  Robert F. Bulleit ,  Dieter Klaubert ,  Mark McDougall ,  Chad Zimprich

IPC分类号： G01N33/53 ,  C12Q1/34 ,  C12N9/14 ,  C12P21/02 ,  C07C19/00 ,  C07C21/00

CPC分类号： C12Q1/34 ,  C07C217/08 ,  C07C271/16 ,  C07D209/48 ,  C07D233/64 ,  C07D311/78 ,  C07D311/82 ,  C07D405/06 ,  C07D493/10 ,  C07D495/04 ,  C07F5/022 ,  C12N9/14 ,  C12N9/86 ,  C12Y308/01005 ,  G01N33/5005 ,  G01N33/58 ,  Y10S436/80

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

公开(公告)号：US08071328B2

公开(公告)日：2011-12-06

申请号：US12706078

申请日：2010-02-16

申请人： Martha O'Brien ,  Keith V. Wood ,  Dieter Klaubert ,  William Daily

发明人： Martha O'Brien ,  Keith V. Wood ,  Dieter Klaubert ,  William Daily

IPC分类号： C12Q1/37

CPC分类号： C07D277/66 ,  C12Q1/37 ,  C12Q1/66

摘要： A sensitive bioluminescent assay to detect proteases including caspases, trypsin and tryptase is provided.

摘要翻译： 提供了敏感的生物发光测定法来检测蛋白酶，包括胱天蛋白酶，胰蛋白酶和类胰蛋白酶。

公开(公告)号：US20110207195A1

公开(公告)日：2011-08-25

申请号：US13021295

申请日：2011-02-04

申请人： Aldis Darzins ,  Lance Encell ,  Tonny Johnson ,  Dieter Klaubert ,  Georgyi V. Los ,  Mark McDougall ,  Keith V. Wood ,  Monika G. Wood ,  Chad Zimprich

发明人： Aldis Darzins ,  Lance Encell ,  Tonny Johnson ,  Dieter Klaubert ,  Georgyi V. Los ,  Mark McDougall ,  Keith V. Wood ,  Monika G. Wood ,  Chad Zimprich

IPC分类号： C12N11/00

CPC分类号： C12N9/14 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  C07K2299/00 ,  C12N9/0069 ,  C12Q1/26 ,  C12Q1/34 ,  C12Q1/66 ,  C12Y113/12005 ,  C12Y308/01005

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代 野生型水解酶。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

公开(公告)号：US20110171673A1

公开(公告)日：2011-07-14

申请号：US13046297

申请日：2011-03-11

申请人： Aldis Darzins ,  Lance Encell ,  Dieter Klaubert ,  Georgyi V. Los ,  Mark McDougall ,  Keith V. Wood ,  Monika G. Wood ,  Chad Zimprich

发明人： Aldis Darzins ,  Lance Encell ,  Dieter Klaubert ,  Georgyi V. Los ,  Mark McDougall ,  Keith V. Wood ,  Monika G. Wood ,  Chad Zimprich

IPC分类号： C12Q1/34

CPC分类号： G01N33/581 ,  C07K7/06 ,  C07K16/00 ,  C07K2317/622 ,  C07K2319/43 ,  C07K2319/61 ,  C09B1/00 ,  C09B11/24 ,  C09B23/04 ,  C09B23/06 ,  C09B23/105 ,  C12N9/14 ,  C12Q1/34 ,  C12Y308/01005 ,  G01N33/573 ,  G01N2333/914 ,  Y10S530/825

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代 野生型水解酶。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

公开(公告)号：US07951550B2

公开(公告)日：2011-05-31

申请号：US11444145

申请日：2006-05-31

申请人： James J. Cali ,  William Daily ,  Erika Hawkins ,  Dieter Klaubert ,  Jianquan Liu ,  Poncho Meisenheimer ,  Michael Scurria ,  John W. Shultz ,  James Unch ,  Michael P. Valley ,  Keith V. Wood ,  Wenhui Zhou

发明人： James J. Cali ,  William Daily ,  Erika Hawkins ,  Dieter Klaubert ,  Jianquan Liu ,  Poncho Meisenheimer ,  Michael Scurria ,  John W. Shultz ,  James Unch ,  Michael P. Valley ,  Keith V. Wood ,  Wenhui Zhou

IPC分类号： C12Q1/66 ,  C12Q1/26 ,  G01N33/573

CPC分类号： C07D277/66 ,  C07D277/68 ,  C07D311/16 ,  C07D417/04 ,  C07D417/14 ,  C07D493/10 ,  C07F9/65583 ,  C09B11/24 ,  C09B19/00 ,  C09B57/00 ,  C09B57/02 ,  C12Q1/66 ,  G01N2500/00

摘要： A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided.

摘要翻译： 提供了检测采用荧光素衍生物或荧光团衍生物的样品中至少一种分子的存在或量的方法。