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31.发明申请公开(公告)号：US20110009807A1
公开(公告)日：2011-01-13
申请号：US12836163
申请日：2010-07-14
IPC分类号： A61N1/30
摘要： This invention comprises an improved electroporation electrode system comprising a single needle and a ring or donut shaped electrode wherein the difference in surface area of the electrodes provide for a substantial reduction of current density near the surface of the treated tissue and a more concentrated current density sufficient for electroporation only in tissues adjacent to the terminal portion of the single needle electrode. Thus, this invention provides for targeting specific tissue for electroporation and also should provide for lessening the sensation of electric current in the treated tissue.
摘要翻译： 本发明包括改进的电穿孔电极系统，其包括单针和环形或环形电极，其中电极的表面面积的差异大大降低了处理组织表面附近的电流密度，并且更集中的电流密度足够 仅在与单针电极的末端部分相邻的组织中进行电穿孔。 因此，本发明提供靶向用于电穿孔的特定组织，并且还应当提供减少处理组织中电流的感觉。
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公开(公告)号：US20070128708A1
公开(公告)日：2007-06-07
申请号：US11636167
申请日：2006-12-07
申请人： Andre Gamelin
发明人： Andre Gamelin
摘要： Disclosed is a chamber apparatus for electroporating in vitro relatively large volumes of a fluid medium carrying biological-cells-or-vesicles wherein-a reservoir for carrying said cells and vesicles is variable in its volume on demand and wherein the volume chosen is directly related to the volume of the sample to be electroporated. The apparatus has further embodiments wherein the chamber is disposable and can be operated either in isolation from a patient or connected thereto.
摘要翻译： 公开了一种用于在体外电穿孔相对较大体积的携带生物细胞或囊泡的流体介质的室装置，其中用于携带所述细胞和囊泡的储存器在其体积上是可变的，并且其中所选择的体积与 要电穿孔的样品的体积。 该装置具有另外的实施例，其中腔室是一次性的，并且可以与患者隔离或连接到其上进行操作。
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33.发明授权Intradermal delivery of active agents by needle-free injection and electroporation 有权通过无针注射和电穿孔活性剂的皮内递送公开(公告)号：US07171264B1
公开(公告)日：2007-01-30
申请号：US10339708
申请日：2003-01-08
IPC分类号： A61N1/30
CPC分类号： A61N1/0424 , A61N1/0428 , A61N1/325
摘要： Methods are proved for introducing a biologically active agent into cells of a subject by introducing the agent in a form suitable for electrotransport into a region of tissue of the subject using one or more needle-free injectors, and applying a pulsed electric field to the region of tissue, thereby causing electroporation of the region of tissue. The combination of needle-free injection and electroporation is sufficient to introduce the agent into cells in skin, muscle or mucosa. For example, the region of tissue can be contacted with two oppositely charged injectors, one acting as the donor electrode and one acting as the counter electrode, or a single injector and one or more electrodes can be used. In addition, needle-free injection may be used in combination with suitable non-invasive electrode configurations. The active agents delivered into cells using the invention method can be small molecules, polynucleotides, polypeptides, and the like.
摘要翻译： 证明通过使用一种或多种无针注射器将适合于电转移到受试者组织区域的形式引入生物活性剂来引入生物活性剂的方法，以及向该区域施加脉冲电场 的组织，从而引起组织区域的电穿孔。 无针注射和电穿孔的组合足以将药剂引入皮肤，肌肉或粘膜中的细胞中。 例如，组织区域可以与两个相反电荷的注射器接触，一个充当施主电极，另一个充当对电极，或者可以使用单个注射器和一个或多个电极。 此外，无针注射可以与合适的非侵入性电极结构组合使用。 使用本发明方法递送到细胞中的活性剂可以是小分子，多核苷酸，多肽等。
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34.发明申请Enhanced delivery of naked DNA to skin by non-invasive in vivo electroporation 有权通过非侵入性体内电穿孔增强裸DNA的表达公开(公告)号：US20060084938A1
公开(公告)日：2006-04-20
申请号：US11291459
申请日：2005-11-30
申请人： Lei Zhang , Dietmar Rabussay
发明人： Lei Zhang , Dietmar Rabussay
IPC分类号： A61M31/00
摘要： In vivo methods are provided for using an electric field to delivery therapeutic or immunizing treatment to a subject by applying non-invasive, user-friendly electrodes to the surface of the skin. Thus, therapeutic or immunizing agents can be delivered into cells of skin for local and systemic treatments or for immunization with optimal gene expression and minimal tissue damage. In particular, therapeutic agents include naked or formulated nucleic acid, polypeptides and chemotherapeutic agents.
摘要翻译： 提供体内方法，通过将非侵入性的，用户友好的电极施加到皮肤表面，使用电场来传递对受试者的治疗或免疫治疗。 因此，治疗或免疫剂可以递送到皮肤细胞中用于局部和全身治疗或用最佳基因表达和最小组织损伤进行免疫。 特别地，治疗剂包括裸体或配制的核酸，多肽和化学治疗剂。
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公开(公告)号：US20020045853A1
公开(公告)日：2002-04-18
申请号：US10020531
申请日：2001-12-14
申请人： GENETRONICS, INC.
IPC分类号： A61M029/00
CPC分类号： A61N1/327 , A61N1/306 , A61N1/325 , Y10S977/906
摘要： A method for sustained intravascular delivery via electroporation is provided. The method is useful for delivery of therapeutic compositions such as antithrombotic and anticoagulant agents. The invention also provides a catheter apparatus for introducing a composition into at least one cell in a vessel in a subject.
摘要翻译： 提供了通过电穿孔持续血管内递送的方法。 该方法可用于递送治疗组合物如抗血栓形成剂和抗凝血剂。 本发明还提供一种用于将组合物引入受试者的血管中的至少一个细胞的导管装置。
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36.发明申请公开(公告)号：US20020019052A1
公开(公告)日：2002-02-14
申请号：US09974882
申请日：2001-10-10
申请人： GENETRONICS, INC.
IPC分类号： C12N015/87 , A61K048/00
CPC分类号： C12N15/87 , C12M35/02 , G01N33/6887
摘要： The present invention provides methods and apparatus for the delivery of at least one chromosome into a cell. Invention methods and apparatus employ FACS or MACS technology for rapidly processing cells and for confirming the introduction of chromosome(s) into a cell. The introduction of the chromosome(s) into the cell is mediated by one or more of a laser, a linear accelerator or electrically induced fusion of a cell and encapsulated chromosome(s). Invention methods provide for the rapid and reliable processing associated with FACS and MACS technology to process thousands of cells a minute, thereby enabling large scale gene transfer.
摘要翻译： 本发明提供了将至少一种染色体递送到细胞中的方法和装置。 本发明的方法和装置采用FACS或MACS技术来快速处理细胞并确认染色体在细胞中的引入。 将染色体引入细胞是通过激光，线性加速器或电诱导的细胞融合和包被的染色体中的一种或多种来介导的。 本发明方法提供了与FACS和MACS技术相关的快速可靠的处理，每分钟处理数千个细胞，从而实现大规模的基因转移。
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公开(公告)号：US6150148A
公开(公告)日：2000-11-21
申请号：US176136
申请日：1998-10-21
CPC分类号： C12M35/02
摘要： An electroporation method and apparatus generating and applying an electric field according to a user-specified pulsing and temperature profile scheme. The apparatus includes a cuvette holder with a Peltier device forming part of the electrode structures that form part of the holder. Advantageously, one such pulse includes a low voltage pulse of a first duration, immediately followed by a high voltage of a second duration, immediately followed by a low voltage of a third duration. The low voltage electroporation field accumulates molecules at the surface of a cell, the appropriately high voltage field creates an opening in the cell, and the final low voltage field moves the molecule into the cell. The molecules may be DNA, portions of DNA, chemical agents, the receiving cells may be eggs, platelets, human cells, red blood cells, mammalian cells, plant protoplasts, plant pollen, liposomes, bacteria, fungi, yeast, sperm, or other suitable cells. The molecules are placed in close proximity to the cells, either in the interstitial space in tissue surrounding the cells or in a fluid medium containing the cells.
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公开(公告)号：US6096020A
公开(公告)日：2000-08-01
申请号：US126941
申请日：1998-07-30
申请人： Gunter A. Hofmann
发明人： Gunter A. Hofmann
摘要： An electroporation method and apparatus generating and applying an electric field according to a user-specified pulsing scheme. Advantageously, one such pulse includes a low voltage pulse of a first duration, immediately followed by a high voltage of a second duration, immediately followed by a low voltage of a third duration. The low voltage electroporation field accumulates molecules at the surface of a cell, the appropriately high voltage field creates an opening in the cell, and the final low voltage field moves the molecule into the cell. The molecules may be DNA, portions of DNA, chemical agents, the receiving cells may be eggs, platelets, human cells, red blood cells, mammalian cells, plant protoplasts, plant pollen, liposomes, bacteria, fungi, yeast, sperm, or other suitable cells. The molecules are placed in close proximity to the cells, either in the interstitial space in tissue surrounding the cells or in a fluid medium containing the cells.
摘要翻译： 根据用户指定的脉冲方案产生和施加电场的电穿孔方法和装置。 有利地，一个这样的脉冲包括第一持续时间的低电压脉冲，紧接着是第二持续时间的高电压，紧接着是第三持续时间的低电压。 低电压电场在电池表面积聚分子，适当的高电压场在电池中产生开口，最终的低电压场将分子移动到电池中。 分子可以是DNA，DNA的一部分，化学试剂，接收细胞可以是蛋，血小板，人细胞，红细胞，哺乳动物细胞，植物原生质体，植物花粉，脂质体，细菌，真菌，酵母，精子或其他 合适的细胞。 将分子放置在细胞附近，或者在围绕细胞的组织的间质空间中或在含有细胞的流体培养基中。
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公开(公告)号：US6027488A
公开(公告)日：2000-02-22
申请号：US90471
申请日：1998-06-03
摘要： A method and apparatus for introducing a preselected molecule into a living cell by contacting the cell with the preselected molecule and applying a multiple series of electrical pulses to the cell. The method can be utilized ex vivo. The multiple electrical pulses generate rotating electric fields which introduce transient pores in the living cell without killing the cell. The rotating electric fields are provided in a flow through chamber apparatus having more than two electrodes. A three-step pulse process, e.g. collection, electroporation, electrophoresis, is used to introduce the preselected molecule into the cell. A mechanical means of repositioning cells between successive pulses is also provided. The apparatus can also provide a means to pulse cells at different temperatures and then after pulsing, let the cells recover for a specified residence time at another temperature.
摘要翻译： 一种通过使细胞与预选分子接触并向细胞施加多系列电脉冲将预选分子引入活细胞的方法和装置。 该方法可以离体使用。 多个电脉冲产生旋转电场，其在活细胞中引入瞬时孔而不杀死细胞。 旋转电场设置在具有多于两个电极的流通室装置中。 三步脉冲处理，例如 收集，电穿孔，电泳，用于将预选分子引入细胞。 还提供了在连续脉冲之间重新定位电池的机械装置。 该装置还可以提供在不同温度下脉冲细胞的手段，然后在脉冲之后，让细胞在另一个温度恢复指定的停留时间。
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公开(公告)号：US5859327A
公开(公告)日：1999-01-12
申请号：US517914
申请日：1995-08-22
申请人： S. B. Dev , Yasuhiko Hayakawa
发明人： S. B. Dev , Yasuhiko Hayakawa
CPC分类号： C12N15/8206
摘要： A method for producing a genetically modified plant by introducing a polynucleotide to an intact plant or plant cell(s) via electroporation, in the absence of cell wall-degrading enzymes. Genetically engineered plants produced by the method of the invention are also provided. The invention also provides a method for producing a polypeptide in an intact plant cell, including plant tissue or a whole plant by introducing a biologically active polypeptide directly into the plant.
摘要翻译： 在不存在细胞壁降解酶的情况下通过电穿孔将多核苷酸引入完整植物或植物细胞来生产遗传修饰植物的方法。 还提供了通过本发明的方法生产的基因工程植物。 本发明还提供了通过将生物活性多肽直接引入植物中来在完整植物细胞中产生多肽的方法，包括植物组织或整株植物。
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