公开(公告)号：US20240254512A1

公开(公告)日：2024-08-01

申请号：US18559921

申请日：2022-05-11

Applicant: FLAGSHIP PIONEERING INNOVATIONS V, INC.

Inventor： Nathan Lawrence Yozwiak ,  Simeon Ungerleider Springer ,  Cesar A. Arze ,  Kristian Graugaard Andersen

IPC: C12N15/86 ,  C07K14/005

CPC classification number: C12N15/86 ,  C07K14/005 ,  C12N2750/00022 ,  C12N2750/00043 ,  C12N2750/00051

Abstract: This invention relates generally to anellovectors and compositions and uses thereof.

公开(公告)号：US20190211361A1

公开(公告)日：2019-07-11

申请号：US16366571

申请日：2019-03-27

Applicant: FLAGSHIP PIONEERING INNOVATIONS V, INC.

Inventor： Avak Kahvejian ,  Erica Gabrielle Weinstein ,  Nicholas McCartney Plugis ,  Kevin James Lebo ,  Fernando Martin Diaz ,  Dhananjay Maniklal Nawandar

IPC: C12N15/86 ,  C12N15/113 ,  C07K14/005

CPC classification number: C12N15/86 ,  C07K14/005 ,  C12N15/113 ,  C12N2310/141 ,  C12N2320/32 ,  C12N2750/00021 ,  C12N2750/00022 ,  C12N2750/00043

Abstract: This invention relates generally to pharmaceutical compositions and preparations of curons and uses thereof.

公开(公告)号：US09879282B2

公开(公告)日：2018-01-30

申请号：US15224984

申请日：2016-08-01

Applicant: VIROVEK, INC.

Inventor： Haifeng Chen

IPC: C12N15/00 ,  C12N15/86 ,  C12N7/00 ,  C12N15/85 ,  C07K14/005

CPC classification number: C12N15/86 ,  C07K14/005 ,  C12N7/00 ,  C12N15/85 ,  C12N2750/00043 ,  C12N2750/00051 ,  C12N2750/14151 ,  C12N2750/14152 ,  C12N2800/105 ,  C12N2800/50 ,  C12N2830/42 ,  C12N2840/20

Abstract: The present teachings disclose nucleic acid cassettes for expressing in an insect cell a plurality of polypeptides encoded by a gene comprising overlapping open reading frames (ORFs). A cassette comprises, in 5′ to 3′ order, a) a first insect cell-operable promoter, b) a 5′ portion of a gene comprising a first ORF of the gene, c) an intron comprising a second insect cell-operable promoter, and d) a 3′ portion of the gene comprising at least one additional ORF. Vectors and insect cells comprising the cassettes are also disclosed, as well as methods for production of recombinant adeno-associated virus in insect cells using the cassettes.

公开(公告)号：US20160333375A1

公开(公告)日：2016-11-17

申请号：US15224984

申请日：2016-08-01

Applicant: VIROVEK, INC.

Inventor： Haifeng Chen

IPC: C12N15/86 ,  C07K14/005 ,  C12N7/00

CPC classification number: C12N15/86 ,  C07K14/005 ,  C12N7/00 ,  C12N15/85 ,  C12N2750/00043 ,  C12N2750/00051 ,  C12N2750/14151 ,  C12N2750/14152 ,  C12N2800/105 ,  C12N2800/50 ,  C12N2830/42 ,  C12N2840/20

Abstract: The present teachings disclose nucleic acid cassettes for expressing in an insect cell a plurality of polypeptides encoded by a gene comprising overlapping open reading frames (ORFs). A cassette comprises, in 5′ to 3′ order, a) a first insect cell-operable promoter, b) a 5′ portion of a gene comprising a first ORF of the gene, c) an intron comprising a second insect cell-operable promoter, and d) a 3′ portion of the gene comprising at least one additional ORF. Vectors and insect cells comprising the cassettes are also disclosed, as well as methods for production of recombinant adeno-associated virus in insect cells using the cassettes.

公开(公告)号：US09382552B2

公开(公告)日：2016-07-05

申请号：US14514606

申请日：2014-10-15

Applicant: PLASMIDFACTORY GMBH & CO. KG

Inventor： Martin Schleef

IPC: C12N15/63 ,  C12N15/86

CPC classification number: C12N15/86 ,  C12N2740/10052 ,  C12N2750/00043 ,  C12N2750/14152 ,  C12N2770/00043 ,  C12N2800/107 ,  C12N2800/30 ,  C12N2800/40 ,  C12N2800/50

Abstract: The invention relates to a minicircle transfer vector for producing viral vectors comprising a transfer sequence and specific packing signals flanking both sides of the transfer sequence for packaging of the transfer sequence into particles of a viral vector. The invention also relates to minicircle packaging vectors carrying support functions for producing viral vectors. The invention further relates to cells bearing the disclosed minicircles. The invention further relates to methods for producing viral vectors using such minicircles and viral vectors obtained thereby, as well as kits useful in performing the described methods.

Abstract translation: 本发明涉及用于产生病毒载体的小环转移载体，其包含转移序列和转移序列两侧侧翼的特异性包装信号，用于将转移序列包装到病毒载体的颗粒中。 本发明还涉及携带用于产生病毒载体的支持功能的小环包装载体。 本发明还涉及带有所公开的微型圆筒的电池。 本发明还涉及使用这样的小环和由此获得的病毒载体产生病毒载体的方法，以及可用于实施所述方法的试剂盒。

公开(公告)号：US20130315944A1

公开(公告)日：2013-11-28

申请号：US13840805

申请日：2013-03-15

Applicant: Virginia Tech Intellectual Properties, Inc.

Inventor： Xiang-Jin MENG ,  Yaowei HUANG

IPC: C07K14/005 ,  C12N7/00 ,  G01N33/569 ,  C12N15/86

CPC classification number: G01N33/56983 ,  A61K39/12 ,  A61K2039/53 ,  A61K2039/552 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2750/00021 ,  C12N2750/00022 ,  C12N2750/00034 ,  C12N2750/00043 ,  C12Q1/701 ,  G01N2333/01 ,  G01N2469/20

Abstract: The present invention also provides infectious DNA clones, biologically functional plasmid or viral vector containing the infectious nucleic acid genome molecule of Torque teno sus virus (TTsuV). The present invention also provides methods for diagnosing TTsuV infection via immunological methods, e.g., enzyme-linked immunoabsorbent assay (ELISA) and Western blot using PTTV specific antigens for detecting serum PTTV specific antibodies which indicate infections TTsuV1, TTsuV2, and individual TTsuV1 genotypes.

Abstract translation: 本发明还提供感染性DNA克隆，含有Torque teno sus病毒（TTsuV）的感染性核酸基因组分子的生物功能质粒或病毒载体。 本发明还提供了通过免疫学方法例如酶联免疫吸附测定（ELISA）和使用PTTV特异性抗原检测表明感染TTsuV1，TTsuV2和个体TTsuV1基因型的血清PTTV特异性抗体的蛋白质印迹来诊断TTsuV感染的方法。

公开(公告)号：US20110318363A1

公开(公告)日：2011-12-29

申请号：US12821634

申请日：2010-06-23

Applicant: Harald zur Hausen ,  Ethel-Michele de Villiers

Inventor： Harald zur Hausen ,  Ethel-Michele de Villiers

IPC: A61K39/42 ,  C12N15/63 ,  C12N7/00 ,  C07K14/01 ,  G06F17/30 ,  C12Q1/70 ,  A61K31/713 ,  A61K39/12 ,  A61P31/12 ,  C07H21/04 ,  C07K16/08

CPC classification number: C12N15/86 ,  C07K14/005 ,  C12N7/00 ,  C12N2750/00021 ,  C12N2750/00022 ,  C12N2750/00034 ,  C12N2750/00043 ,  C12Q1/701

Abstract: Described are single-stranded new sequences of TT viruses, rearranged TTV sequences and hybrid molecules of a specific TT virus sequence and host cell DNA that are capable of replicating autonomously for use in diagnosis, prevention and treatment of diseases like cancer and autoimmunity. In addition, it relates to the use of such molecules as gene vectors and artificial chromosomes.

Abstract translation: 描述了TT病毒的单链新序列，重排的TTV序列和特异性TT病毒序列的混合分子以及能够自主复制用于诊断，预防和治疗诸如癌症和自身免疫的疾病的宿主细胞DNA。 此外，它涉及使用这样的分子作为基因载体和人造染色体。

公开(公告)号：US20090203071A1

公开(公告)日：2009-08-13

申请号：US12297958

申请日：2007-08-24

Applicant: Haifeng Chen

Inventor： Haifeng Chen

IPC: C12P21/06 ,  C12P19/34 ,  C12N7/00 ,  C12N5/10 ,  C12N15/63 ,  C07H21/04

CPC classification number: C12N15/86 ,  C07K14/005 ,  C12N7/00 ,  C12N15/85 ,  C12N2750/00043 ,  C12N2750/00051 ,  C12N2750/14151 ,  C12N2750/14152 ,  C12N2800/105 ,  C12N2800/50 ,  C12N2830/42 ,  C12N2840/20

Abstract: The present teachings disclose nucleic acid cassettes for expressing in an insect cell a plurality of polypeptides encoded by a gene comprising overlapping open reading frames (ORFs). A cassette comprises, in 5′ to 3′ order, a) a first insect cell-operable promoter, b) a 5′ portion of a gene comprising a first ORF of the gene, c) an intron comprising a second insect cell-operable promoter, and d) a 3′ portion of the gene comprising at least one additional ORF. Vectors and insect cells comprising the cassettes are also disclosed, as well as methods for production of recombinant adeno-associated virus in insect cells using the cassettes.

Abstract translation: 本教导公开了用于在昆虫细胞中表达由包含重叠开放阅读框（ORFs）的基因编码的多个多肽的核酸盒。 一个盒子以5'至3'的顺序包括a）第一个昆虫细胞可操作的启动子，b）包含该基因的第一个ORF的基因的5'部分，c）包含第二个昆虫细胞可操作的启动子 启动子，和d）包含至少一个额外ORF的基因的3'部分。 还公开了包含盒的载体和昆虫细胞，以及在使用盒的昆虫细胞中产生重组腺相关病毒的方法。

公开(公告)号：US20050221293A1

公开(公告)日：2005-10-06

申请号：US10925363

申请日：2004-08-24

Applicant: Thomas Tuschl ,  Sebastien Pfeffer

Inventor： Thomas Tuschl ,  Sebastien Pfeffer

IPC: C07H21/04 ,  C12N7/00 ,  C12N15/86 ,  C12Q1/68 ,  C12Q1/70

CPC classification number: C12N15/86 ,  C12N7/00 ,  C12N2750/00021 ,  C12N2750/00043

Abstract: The invention relates to isolated nucleic acid molecules comprising the sequence of any one of the DNA virus microRNAs shown in Table A1. In another embodiment, the invention relates to single stranded DNA virus microRNA molecules and anti-DNA virus microRNA molecules.

Abstract translation: 本发明涉及分离的核酸分子，其包含表A1所示的任何一种DNA病毒微小RNA的序列。 在另一个实施方案中，本发明涉及单链DNA病毒微小RNA分子和抗DNA病毒微小RNA分子。