公开(公告)号：US20200010483A1

公开(公告)日：2020-01-09

申请号：US16487845

申请日：2018-02-13

Applicant: NANJING FORESTRY UNIVERSITY

Inventor： Li XU ,  Shilong YANG ,  Zhong LI

IPC: C07F1/00

Abstract: A biflavone-copper complex, a preparation method and application thereof are disclosed. The biflavone-copper complex has the following structural formula: wherein, X is NO3− or Cl−. A method of preparing the biflavone-copper complex includes the following steps: dissolving a copper salt in an alcohol and then adding into a biflavone alcoholic solution, controlling pH to 5-7; under heating and stirring, performing a reaction for 2-5 h to form a precipitate; filtering the precipitate, washing the precipitate with alcohol and water, recrystallizing the precipitate using dimethyl sulfoxide as a solvent, and drying the precipitate to obtain the biflavone-copper complex. The biflavone-copper complex is used for preparing an antitumor drug and/or an antioxidant drug.

公开(公告)号：US10260995B2

公开(公告)日：2019-04-16

申请号：US15807452

申请日：2017-11-08

Applicant: NANJING FORESTRY UNIVERSITY ,  ZHENGZHOU UNIVERSITY

Inventor： Zeli Que ,  Xiaolin Yang ,  Qicheng Teng ,  Hongyi Lu ,  Feibin Wang ,  Zherui Li ,  Weizhen Cai

IPC: G01M99/00 ,  G01N3/08 ,  G01L5/24

Abstract: A test device of universal nail/screw holding power is provided. The device is capable of penetrating a nail/screw into a test piece at any angle and positioning the test piece on which a nail/screw is penetrated at any angle to test the nail/screw holding power. The device includes a base, two opposite supporting plates, two coaxial disks, an upper bearing plate, a lower bearing plate, a side bearing plate, a through groove, a trapezoid slot, and a plurality of trapezoid bolts on the same circumference. When the disk is turned, at least one trapezoid bolt is inserted into the trapezoid slot; when the at least one trapezoid bolt engages with the trapezoid slot and then is tightened up, the at least one trapezoid bolt and the trapezoid slot cooperatively limit the turn of the disk.

公开(公告)号：US10212893B2

公开(公告)日：2019-02-26

申请号：US15106260

申请日：2015-07-08

Applicant: NANJING FORESTRY UNIVERSITY

Inventor： Qingqing Shang ,  Tongming Yin ,  Zhiwu Sun ,  Yunwei Ju

IPC: A01G7/06 ,  A01G23/00 ,  A01G29/00

Abstract: A trunk injection needle includes: a front part which is conical, a middle part which is cylindrical, and a rear part which is cylindrical, which are respectively provided along a needle axis; wherein an outer diameter of the rear part is larger than outer diameters of the front part and the middle part; the front part has a bits guiding hole extending along the needle axis, and the rear part has a drug feeding hole extending along the needle axis; wherein the middle part has two outlet holes extending along a radical direction, and a separating board is provided between the two outlet holes; a rear end of the bits guiding hole communicates with the two outlet holes at two sides of the separating board; and a front end of the drug feeding hole communicates with the two outlet holes.

公开(公告)号：US20160097457A1

公开(公告)日：2016-04-07

申请号：US14894487

申请日：2014-04-21

Applicant: NANJING FORESTRY UNIVERSITY

Inventor： Jiajun Sun ,  Qiong Hu ,  Min Zhou ,  Qiaoan Tu ,  Chenbo Ma ,  Bo Yu

IPC: F16J15/34 ,  F16J15/00

CPC classification number: F16J15/3412 ,  F16J15/002 ,  F16J15/342

Abstract: A self-pumping hydrodynamic mechanical seal includes a rotating ring (3) and a stationary ring (11). More than three sets of backward curved grooves (39) are provided on a seal face of the rotating ring (3), outlets of the backward curved grooves (39) are provided at an external diameter portion of the seal face of the rotating ring (3), and an inlet (31) connects with a seal chamber (1) through a duct (30) of the rotating ring (3) or the stationary ring (11). When the rotating ring (3) rotates, a medium in the backward curved grooves (39) is accelerated into a high-speed fluid; under a centrifugal force, the high-speed fluid moves to an external diameter side of the rotating ring (3), so as to be pumped into the seal chamber (1) and generates a low-pressure area at the inlets (31) of the backward curved grooves (39); the medium in the seal chamber (1) is driven by a pressure difference, so as to flow into the backward curved grooves (39) through the duct (30), for forming circulation of self-pumping. During pumping the high-speed fluid, a flow speed of the high-speed fluid is slowed and a pressure of the high-speed fluid is increased, as a flow sectional area of the backward curved grooves (39) is increased, so as to generate an opening force which separates the rotating ring (3) from the stationary ring (11). The seal has desirable capabilities of self-lubrication, self-flushing, solid particle interference resistance, and optimal sealing performance.

Abstract translation: 自抽水力机械密封件包括旋转环（3）和固定环（11）。 在旋转环（3）的密封面上设置有三组后向弯曲槽（39），后向弯曲槽（39）的出口设置在旋转环的密封面的外径部 3），入口（31）通过旋转环（3）或固定环（11）的管道（30）与密封室（1）连接。 当旋转环（3）旋转时，向后弯曲槽（39）中的介质被加速成高速流体; 在离心力作用下，高速流体移动到旋转环（3）的外径侧，以便被泵送到密封室（1）中并且在入口（31）处产生低压区域 向后弯曲的凹槽（39）; 密封室（1）中的介质由压力差驱动，从而通过管道（30）流入后向弯曲槽（39），以形成自抽水循环。 在泵送高速流体期间，随着后向弯曲槽（39）的流动截面面积增加，高速流体的流速减慢，高速流体的压力增加，从而 产生将旋转环（3）与固定环（11）分离开的打开力。 密封件具有自润滑，自冲洗，坚固的颗粒抗干扰性和最佳密封性能所需的能力。

公开(公告)号：US12287353B2

公开(公告)日：2025-04-29

申请号：US18844070

申请日：2023-08-31

Applicant: NANJING FORESTRY UNIVERSITY

Inventor： Caoxing Huang ,  Xiaoxue Zhao ,  Qiang Yong ,  Chenhuan Lai ,  Yongcan Jin

IPC: G01Q60/38 ,  C12Q1/40 ,  G01Q60/28 ,  G01Q60/42

Abstract: A method for characterizing the interaction force between lignin and cellulase is provided. The method includes the following steps: (1) preparing a lignin solution, then dropwise adding the solution onto a silicon wafer, repeatedly spin-coating with a spin coater, and vacuum-drying the prepared lignin film; (2) modifying an AFM probe coated with a gold film on the surface with HS-PEG-COOH and then activating the carboxyl group, immersing the AFM probe after the carboxyl group activation in a PBS solution of cellulase for incubation, and after the modification is completed, placing the washed AFM probe in a PBS buffer solution for storage for later use; (3) testing the lignin-cellulase interaction force by using an AFM instrument in a liquid phase environment.

公开(公告)号：US20250092412A1

公开(公告)日：2025-03-20

申请号：US18825366

申请日：2024-09-05

Applicant: Nanjing Forestry University

Inventor： Fangfang Fu ,  Liangjiao Xue ,  Fuliang Cao ,  Anqi Zhao ,  Wei Xu ,  Pingjun Xu ,  Xiaoming Yang ,  Meiling Ming

IPC: C12N15/82

Abstract: A Ginkgo biloba wuschel-related homeobox 3A (GbWOX3A) gene and an application thereof in plant tissue culture are provided. The GbWOX3A gene is derived from a Ginkgo biloba embryo, the gene sequence of the GbWOX3A gene is SEQ ID NO: 1, and the amino acid sequence encoded by the GbWOX3A gene is SEQ ID NO: 2. An expression of the GbWOX3A gene in developing embryo of Ginkgo biloba and regenerating a callus has specificity, and transgenic function verification experiments prove that overexpression of the GbWOX3A gene can promote regeneration of adventitious buds during the plant tissue culture. Therefore, the GbWOX3A gene has important theoretical significance and application value in the plant tissue culture, and especially plays an important role in improving the regeneration efficiency of gymnosperms.

公开(公告)号：US20240196904A1

公开(公告)日：2024-06-20

申请号：US17147173

申请日：2021-01-12

Applicant: NANJING FORESTRY UNIVERSITY

Inventor： JIAYAO ZHUANG ,  CHAO LIU ,  XIAOXUE WANG ,  JIAXIN ZHENG ,  KUN TIAN

IPC: A01N63/22 ,  A01N25/02 ,  C12N1/20 ,  C12R1/07

CPC classification number: A01N63/22 ,  A01N25/02 ,  C12N1/205 ,  C12N2500/12 ,  C12N2500/74 ,  C12N2501/998 ,  C12N2523/00 ,  C12N2527/00 ,  C12R2001/07

Abstract: A mixed bacteria for promoting nodulation and nitrogen fixation of Robinia pseudoacacia and their application are provided. The mixed bacteria includes Kocuria sp. X-22, Microbacterium sp. X-26, and Bacillus sp. X-28, all of which have been preserved in China Center for Type Culture Collection, and the preservation numbers respectively are: CCTCC No: M 2019237; CCTCC No: M 2019238; CCTCC No: M 2019239. The mixed bacteria are watered directly around the seedlings of Robinia pseudoacacia. Compared with the single bacteria control group and the sterile control group, the disclosure can produce synergistic superimposing effects, significantly improve the nodulation rate and symbiotic nitrogen fixation of the Robinia pseudoacacia, and promote the photosynthesis of the Robinia pseudoacacia.

公开(公告)号：US20240188515A1

公开(公告)日：2024-06-13

申请号：US17180730

申请日：2023-09-12

Applicant: NANJING FORESTRY UNIVERSITY

Inventor： JIAYAO ZHUANG ,  CHAO LIU ,  GUOHUA FAN ,  KUN TIAN ,  JINCHI ZHANG

IPC: A01G24/60 ,  C05F5/00 ,  C05F11/10

CPC classification number: A01G24/60 ,  C05F5/008 ,  C05F11/10

Abstract: A method for spraying Robinia pseudoacacia on exposed shale wall to efficiently and rapidly restore green and improve soil pH value is provided. External-soil spray seeding is used to spray mixed microorganisms, organic fertilizer, and soil on exposed shale walls with a green plant of Robinia pseudoacacia to efficiently and rapidly restore green and improve soil pH value. The mixed microorganisms include Kocuria sp. X-22, Microbacterium sp. X-26, Bacillus sp. X-28 and Microbacterium sp. X-18, and the mixed microorganisms are added to organic fertilizer and soil by fermentation broth. The weight ratio of mixed microorganisms, organic fertilizer and soil is 1:1:8. The method can promote the rapid growth of Robinia pseudoacacia on the exposed shale wall and significantly increase organic matter content, effective phosphorus content, and pH value of the Robinia pseudoacacia soil.

公开(公告)号：US11999984B2

公开(公告)日：2024-06-04

申请号：US18274770

申请日：2021-05-06

Applicant: NANJING FORESTRY UNIVERSITY

Inventor： Caoxing Huang ,  Qiang Yong ,  Yuheng Tao ,  Chenhuan Lai ,  Yong Xu ,  Xin Li

IPC: C12P19/04 ,  C12N9/24 ,  C12N9/26 ,  C12N9/40

CPC classification number: C12P19/04 ,  C12N9/2465 ,  C12N9/2491 ,  C12Y302/01022 ,  C12Y302/01025

Abstract: A method and special complex enzyme for hydrolyzing a galactomannan (GM) to prepare a small-molecule GM and a galactomannan oligosaccharide (GMOS) is provided. The method includes: conducting fermentation with microcrystalline cellulose (MCC) and melibiose as carbon sources and Trichoderma reesei (T. reesei) as an enzyme-producing strain to obtain a supernatant, which is a complex enzyme solution with enzymatic activities of β-mannanase and α-galactosidase; and directly using the complex enzyme solution for enzymatic hydrolysis of a GM as a substrate to prepare the small-molecule GM and the GMOS.

公开(公告)号：US20230399270A1

公开(公告)日：2023-12-14

申请号：US17503325

申请日：2021-10-17

Applicant: NANJING FORESTRY UNIVERSITY

Inventor： JIAYAO ZHUANG ,  CHAO LIU ,  TONGXIN XU ,  HAO YANG ,  WENTAO ZHANG

IPC: C05F11/08 ,  C12N1/14

CPC classification number: C05F11/08 ,  C12N1/145 ,  C12R2001/80

Abstract: A Penicillium simplicissimum sp. nl-z1 strain for promoting growth of root nodules and increasing abundance of probiotic microorganism population is disclosed. The Penicillium simplicissimum sp. nl-z1 strain can promote Indigofera pseudotinctoria Matsum. production and nodule growth, and improve probiotic microorganisms and nutritional environment. After the application of Penicillium simplicissimum sp. nl-z1, the nutrient elements needed by plants such as potassium, calcium and magnesium in rock powder are effectively released, the fusion of spraying matrix and rock surface is promoted, the nutrition is continuously supplied for plants, the total nodule weight is increased by 152.63%, the content of hydrolyzed nitrogen is improved by 15.28%, and the symbiotic nitrogen fixation ability is effectively exerted. At the same time, the relative abundance of Mortierella in soil is increased from 12.81% to 94.95%, and the increasing amount of Mortierella and its promoting effect on plants are better than that of direct application of Mortierella.