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41.发明申请公开(公告)号：US20060034829A1
公开(公告)日：2006-02-16
申请号：US11187079
申请日：2005-07-21
Applicant: Tillman Gerngross , Huijuan Li , Stefan Wildt
Inventor： Tillman Gerngross , Huijuan Li , Stefan Wildt
IPC: C07K16/28 , C07H21/04 , C12P21/06 , A61K39/395 , C12N5/06
CPC classification number: C07K16/00 , A01K2217/075 , C07K16/2896 , C07K2317/24 , C07K2317/41 , C12N9/1051 , C12P21/005
Abstract: The present invention relates to immunoglobulin glycoprotein compositions having predominant N-glycan structures on an immunoglobulin glycoprotein which confer a specific effector function. Additionally, the present invention relates to pharmaceutical compositions comprising an antibody having a particular enriched N-glycan structure, wherein said N-glycan structure is Man3GlcNAc2.
Abstract translation: 本发明涉及在赋予特异性效应子功能的免疫球蛋白糖蛋白上具有主要N-聚糖结构的免疫球蛋白糖蛋白组合物。 另外，本发明涉及包含具有特定富含N-聚糖结构的抗体的药物组合物，其中所述N-聚糖结构是Man 3 GlcNAc 2。
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42.发明申请Immunoglobulins comprising predominantly a GlcNAc2Man3GlcNAc2 glycoform 审中-公开主要包含GlcNAc2Man3GlcNAc2糖型的免疫球蛋白公开(公告)号：US20060029604A1
公开(公告)日：2006-02-09
申请号：US11187229
申请日：2005-07-21
Applicant: Tillman Gerngross , Huijuan Li , Stefan Wildt
Inventor： Tillman Gerngross , Huijuan Li , Stefan Wildt
IPC: A61K39/395 , C07H21/04 , C12P21/06 , C12N5/06 , C07K16/28
CPC classification number: C07K16/00 , C07K16/2896 , C07K2317/41 , C07K2317/52 , C07K2317/732
Abstract: The present invention relates to immunoglobulin glycoprotein compositions having predominant N-glycan structures on an immunoglobulin glycoprotein which confer a specific effector function. Additionally, the present invention relates to pharmaceutical compositions comprising an antibody having a particular enriched N-glycan structure, wherein said N-glycan structure is GlcNAc2Man3GlcNAc2.
Abstract translation: 本发明涉及在赋予特异性效应子功能的免疫球蛋白糖蛋白上具有主要N-聚糖结构的免疫球蛋白糖蛋白组合物。 另外，本发明涉及药物组合物，其包含具有特定富集的N-聚糖结构的抗体，其中所述N-聚糖结构是GlcNAc 2 N 3 GlcNAc 2 。
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公开(公告)号：US09260712B2
公开(公告)日：2016-02-16
申请号：US13251410
申请日：2011-10-03
Applicant: Bianka Prinz , Natarajan Sethuraman , Dongxing Zha , Stefan Wildt , Piotr Bobrowicz
Inventor： Bianka Prinz , Natarajan Sethuraman , Dongxing Zha , Stefan Wildt , Piotr Bobrowicz
CPC classification number: C12N15/1037 , C07K16/00 , C07K16/2887 , C07K16/32 , C07K2319/30 , C12N15/1034 , G01N33/6854
Abstract: Methods for display of recombinant whole immunoglobulins or immunoglobulin libraries on the surface of eukaryote host cells, including yeast and filamentous fungi, are described. The methods are useful for screening libraries of recombinant immunoglobulins in eukaryote host cells to identify immunoglobulins that are specific for an antigen of interest.
Abstract translation: 描述了在真核生物宿主细胞（包括酵母和丝状真菌）表面上显示重组整个免疫球蛋白或免疫球蛋白文库的方法。 该方法可用于筛选真核生物宿主细胞中重组免疫球蛋白的文库，以鉴定对感兴趣的抗原特异的免疫球蛋白。
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44.发明申请公开(公告)号：US20130295608A1
公开(公告)日：2013-11-07
申请号：US13978714
申请日：2012-02-20
Applicant: Piotr Bobrowicz , William J. Cook , Stephen Hamilton , Juergen Nett , Terrance A. Stadheim , Stefan Wildt
Inventor： Piotr Bobrowicz , William J. Cook , Stephen Hamilton , Juergen Nett , Terrance A. Stadheim , Stefan Wildt
IPC: C07K14/39
CPC classification number: C07K14/39 , C12N9/90 , C12P21/005
Abstract: Lower eukaryotic host cells have been recombinantly engineered to produce glycoprotein having human-like O-glycosylation. The glycoproteins are useful for the production of glycoprotein compositions with advantages for the production of human therapeutics.
Abstract translation: 已经重组设计了较低真核宿主细胞以产生具有人类O-糖基化的糖蛋白。 糖蛋白可用于生产具有用于生产人类治疗剂的优点的糖蛋白组合物。
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公开(公告)号：US08067551B2
公开(公告)日：2011-11-29
申请号：US12291373
申请日：2008-11-07
Applicant: Tillman U. Gerngross , Stefan Wildt , Byung-Kwon Choi , Juergen Hermann Nett , Piotr Bobrowicz , Stephen R Hamilton , Robert C. Davidson
Inventor： Tillman U. Gerngross , Stefan Wildt , Byung-Kwon Choi , Juergen Hermann Nett , Piotr Bobrowicz , Stephen R Hamilton , Robert C. Davidson
IPC: C07K14/00
CPC classification number: C12P21/005 , C07K14/47 , C12N9/1051 , C12N15/1082 , C12N15/79
Abstract: The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man5GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar transporters and mannosidases, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.
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Patent Agency Ranking
46.发明授权Combinatorial DNA library for producing modified N-glycans in lower eukaryotes 有权用于在低等真核生物中生产修饰的N-聚糖的组合DNA文库公开(公告)号：US07935513B2
公开(公告)日：2011-05-03
申请号：US12156936
申请日：2008-06-05
Applicant: Tillman U. Gerngross , Stefan Wildt , Byung-Kwon Choi , Juergen Hermann Nett , Piotr Bobrowicz , Stephen R. Hamilton , Robert C. Davidson
Inventor： Tillman U. Gerngross , Stefan Wildt , Byung-Kwon Choi , Juergen Hermann Nett , Piotr Bobrowicz , Stephen R. Hamilton , Robert C. Davidson
CPC classification number: C12P21/005 , C07K14/47 , C12N9/1051 , C12N15/1082 , C12N15/79
Abstract: The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man5GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar transporters and mannosidases, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.
Abstract translation: 本发明涉及具有修饰的寡糖的真核宿主细胞，其可以通过异源表达一组糖基转移酶，糖转运体和甘露糖苷酶进一步修饰，以成为用于产生哺乳动物例如人治疗性糖蛋白的宿主菌株。 本发明提供核酸分子和组合文库，其可用于成功靶向和表达哺乳动物酶活性，例如参与糖基化的真核宿主细胞中的细胞内区室的那些。 该方法提供了可用于表达和靶向参与糖基化的任何所需基因的工程化宿主细胞。 建立或选择具有修饰寡糖的宿主细胞。 在工程化宿主细胞中制备的N-聚糖具有Man5GlcNAc2核心结构，然后可以通过异源表达一种或多种酶，例如糖基转移酶，糖转运蛋白和甘露糖苷酶来进一步修饰，以产生人样糖蛋白。 为了生产治疗性蛋白质，该方法可适用于工程化可能获得任何所需糖基化结构的细胞系。
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公开(公告)号：US20100009866A1
公开(公告)日：2010-01-14
申请号：US12489900
申请日：2009-06-23
Applicant: Bianka Prinz , Natarajan Sethuraman , Dongxing Zha , Stefan Wildt , Piotr Bobrowicz
Inventor： Bianka Prinz , Natarajan Sethuraman , Dongxing Zha , Stefan Wildt , Piotr Bobrowicz
CPC classification number: C12N15/1037 , C07K16/00 , C07K16/2887 , C07K16/32 , C07K2319/30 , C12N15/1034 , G01N33/6854
Abstract: Methods for display of recombinant whole immunoglobulins or immunoglobulin libraries on the surface of eukaryote host cells, including yeast and filamentous fungi, are described. The methods are useful for screening libraries of recombinant immunoglobulins in eukaryote host cells to identify immunoglobulins that are specific for an antigen of interest.
Abstract translation: 描述了在真核生物宿主细胞（包括酵母和丝状真菌）表面上显示重组整个免疫球蛋白或免疫球蛋白文库的方法。 该方法可用于筛选真核生物宿主细胞中重组免疫球蛋白的文库，以鉴定对感兴趣的抗原特异的免疫球蛋白。
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48.发明授权公开(公告)号：US07598055B2
公开(公告)日：2009-10-06
申请号：US10680963
申请日：2003-10-07
Applicant: Piotr Bobrowicz , Stephen R. Hamilton , Tillman U. Gerngross , Stefan Wildt , Byung-Kwon Choi , Juergen H. Nett , Robert C. Davidson
Inventor： Piotr Bobrowicz , Stephen R. Hamilton , Tillman U. Gerngross , Stefan Wildt , Byung-Kwon Choi , Juergen H. Nett , Robert C. Davidson
CPC classification number: C07K14/39 , A61K38/00 , C07K2319/05 , C12N9/1051 , C12P21/005 , Y02A50/396
Abstract: The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified lipid-linked oligosaccharides are created or selected. N-glycans made in the engineered host cells exhibit GnTIII activity, which produce bisected N-glycan structures and may be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar transporters and mannosidases, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.
Abstract translation: 本发明涉及具有修饰的寡糖的真核宿主细胞，其可以通过异源表达一组糖基转移酶，糖转运体和甘露糖苷酶进一步修饰，以成为用于产生哺乳动物例如人治疗性糖蛋白的宿主菌株。 该方法提供了可用于表达和靶向参与糖基化的任何所需基因的工程化宿主细胞。 制备或选择具有修饰的脂质连接寡糖的宿主细胞。 在工程化的宿主细胞中制备的N-聚糖表现出GnTIII活性，其产生二等分的N-聚糖结构，并且可以通过异源表达一种或多种酶（例如糖基转移酶，糖转运蛋白和甘露糖苷酶）进一步修饰，以产生人样糖蛋白。 为了生产治疗性蛋白质，该方法可适用于工程化可能获得任何所需糖基化结构的细胞系。
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49.发明申请Immunoglobulins Comprising Predominantly a Glcnacman3Glcnac2 Glycoform 审中-公开免疫球蛋白主要包含Glcnacman3Glcnac2 Glycoform公开(公告)号：US20090136525A1
公开(公告)日：2009-05-28
申请号：US11990722
申请日：2006-09-01
Applicant: Tillman Gerngross , Stefan Wildt , Huijuan Li
Inventor： Tillman Gerngross , Stefan Wildt , Huijuan Li
IPC: A61K39/395 , C12N5/10 , C12P21/00 , A61P37/00
CPC classification number: C07K16/2887 , C07K2317/24 , C07K2317/41 , C07K2317/72
Abstract: Compositions and methods for producing compositions comprising immunoglobulins or immunoglobulin fragments having an N-linked glycosylation pattern consisting predominantly of the GlCNAcMan3GlcNAc2 N-glycan structure are disclosed. The GlCNAcMan3GlcNAc2 N-glycan structure effects an increase in binding to the FcγRiπ receptors and a decrease in binding to the FcγRH receptors.
Abstract translation: 公开了用于生产组合物的组合物和方法，其包含主要由GlCNAcMan 3 GlcNAc 2 N-聚糖结构组成的具有N-连接的糖基化模式的免疫球蛋白或免疫球蛋白片段。 GlCNAcMan3GlcNAc2 N-聚糖结构影响与FcγRipi受体的结合增加，并降低与FcγR受体的结合。
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50.发明申请Immunoglobulins comprising predominantly a GalGlcNAcMan5GLcNAc2 glycoform 审中-公开免疫球蛋白主要包含GalGlcNAcMan5GLcNAc2糖型公开(公告)号：US20060034830A1
公开(公告)日：2006-02-16
申请号：US11187113
申请日：2005-07-21
Applicant: Tillman Gerngross , Huijuan Li , Stefan Wildt
Inventor： Tillman Gerngross , Huijuan Li , Stefan Wildt
IPC: A61K39/395 , C07H21/04 , C12P21/06 , C12N5/06 , C07K16/28
CPC classification number: C07K16/2896 , C07K16/00 , C07K2317/24 , C07K2317/52
Abstract: The present invention relates to immunoglobulin glycoprotein compositions having predominant N-glycan structures on an immunoglobulin glycoprotein which confer a specific effector function. Additionally, the present invention relates to pharmaceutical compositions comprising an antibody having a particular enriched N-glycan structure, wherein said N-glycan structure is GalGlcNAcMan5GlcNAc2.
Abstract translation: 本发明涉及在赋予特异性效应子功能的免疫球蛋白糖蛋白上具有主要N-聚糖结构的免疫球蛋白糖蛋白组合物。 另外，本发明涉及包含具有特定富含N-聚糖结构的抗体的药物组合物，其中所述N-聚糖结构是GalGlcNAcMan 5 GlcNAc 2。
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