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1.发明授权Yeast strain for the production of proteins with modified O-glycosylation 有权用于生产具有修饰的O-糖基化的蛋白质的酵母菌株公开(公告)号：US08936918B2
公开(公告)日：2015-01-20
申请号：US13978714
申请日：2012-02-20
申请人： Piotr Bobrowicz , William J. Cook , Stephen Hamilton , Juergen Nett , Terrance A. Stadheim , Stefan Wildt
发明人： Piotr Bobrowicz , William J. Cook , Stephen Hamilton , Juergen Nett , Terrance A. Stadheim , Stefan Wildt
CPC分类号： C07K14/39 , C12N9/90 , C12P21/005
摘要： Lower eukaryotic host cells have been recombinantly engineered to produce glycoprotein having human-like O-glycosylation. The glycoproteins are useful for the production of glycoprotein compositions with advantages for the production of human therapeutics.
摘要翻译： 已经重组设计了较低真核宿主细胞以产生具有人类O-糖基化的糖蛋白。 糖蛋白可用于生产具有用于生产人类治疗剂的优点的糖蛋白组合物。
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2.发明申请公开(公告)号：US20130295608A1
公开(公告)日：2013-11-07
申请号：US13978714
申请日：2012-02-20
申请人： Piotr Bobrowicz , William J. Cook , Stephen Hamilton , Juergen Nett , Terrance A. Stadheim , Stefan Wildt
发明人： Piotr Bobrowicz , William J. Cook , Stephen Hamilton , Juergen Nett , Terrance A. Stadheim , Stefan Wildt
IPC分类号： C07K14/39
CPC分类号： C07K14/39 , C12N9/90 , C12P21/005
摘要： Lower eukaryotic host cells have been recombinantly engineered to produce glycoprotein having human-like O-glycosylation. The glycoproteins are useful for the production of glycoprotein compositions with advantages for the production of human therapeutics.
摘要翻译： 已经重组设计了较低真核宿主细胞以产生具有人类O-糖基化的糖蛋白。 糖蛋白可用于生产具有用于生产人类治疗剂的优点的糖蛋白组合物。
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3.发明授权Method for producing proteins in Pichia pastoris that lack detectable cross binding activity to antibodies against host cell antigens 有权在巴斯德毕赤酵母中生产蛋白质的方法，其对抗宿主细胞抗原的抗体缺乏可检测的交叉结合活性公开(公告)号：US09139632B2
公开(公告)日：2015-09-22
申请号：US13501350
申请日：2010-10-11
申请人： Piotr Bobrowicz , Sujatha Gomathinayagam , Stephen Hamilton , Huijuan Li , Natarajan Sethuraman , Terrance A. Stadheim , Stefan Wildt
发明人： Piotr Bobrowicz , Sujatha Gomathinayagam , Stephen Hamilton , Huijuan Li , Natarajan Sethuraman , Terrance A. Stadheim , Stefan Wildt
IPC分类号： C12P21/06 , C12N1/00 , C12N5/00 , C12P21/04 , C07K1/00 , C07K14/505 , C07K14/47 , C07K14/485 , C07K14/52 , C07K14/535 , C07K14/54 , C07K14/555 , C07K14/59 , C07K14/605 , C07K14/705 , C07K14/715 , C07K14/755 , C07K14/81 , C07K16/18 , C07K16/22 , C12N9/10 , C12P21/00
CPC分类号： C07K14/505 , C07K14/47 , C07K14/4715 , C07K14/4721 , C07K14/4743 , C07K14/485 , C07K14/52 , C07K14/523 , C07K14/535 , C07K14/54 , C07K14/555 , C07K14/59 , C07K14/605 , C07K14/705 , C07K14/70546 , C07K14/70578 , C07K14/7151 , C07K14/755 , C07K14/8114 , C07K14/8125 , C07K14/8128 , C07K16/18 , C07K16/22 , C07K2317/33 , C12N9/1051 , C12P21/005
摘要： Methods for producing proteins and glycoproteins in Pichia pastoris that lack detectable cross binding activity to antibodies made against host cell antigens are described. In particular, methods are described wherein recombinant Pichia pastoris strains that do not display a β-mannosyltransferase 2 activity with respect to an N-glycan or O-glycan and do not display at least one activity selected from a β-mannosyltransferase 1, 3, and 4 activity to produce recombinant proteins and glycoproteins. These recombinant Pichia pastoris strains can produce proteins and glycoproteins that lack detectable α-mannosidase resistant β-mannose residues thereon and thus, lack cross binding activity to antibodies against host cell antigens. Further described are methods for producing bi-sialylated human erythropoietin in Pichia pastoris that lack detectable cross binding activity to antibodies against host cell antigens.
摘要翻译： 描述了在针对宿主细胞抗原的抗体中缺乏可检测的交联结合活性的巴斯德毕赤酵母中产生蛋白质和糖蛋白的方法。 具体地说，描述了相对于N-聚糖或O-聚糖不显示“ - - 甘露糖基转移酶2”活性的重组毕赤酵母菌株并且不显示至少一种选自β-甘露糖基转移酶1， 3和4活性产生重组蛋白和糖蛋白。 这些重组毕赤酵母菌株可以产生在其上缺乏可检测的α-甘露糖苷酶抗性和甘露糖残基的蛋白质和糖蛋白，因此对抗宿主细胞抗原的抗体缺乏交叉结合活性。 进一步描述了在巴斯德毕赤酵母中生产双唾液酸化人促红细胞生成素的方法，其对抗宿主细胞抗原的抗体缺乏可检测的交联结合活性。
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4.发明申请METHOD FOR PRODUCING PROTEINS IN PICHIA PASTORIS THAT LACK DETECTABLE CROSS BINDING ACTIVITY TO ANTIBODIES AGAINST HOST CELL ANTIGENS 有权在PICHIA PASTORIS中生产蛋白质的方法，将检测到的交叉结合活性与抗宿主细胞抗原抗体公开(公告)号：US20120232007A1
公开(公告)日：2012-09-13
申请号：US13501350
申请日：2010-10-11
申请人： Piotr Bobrowicz , Sujatha Gomathinayagam , Stephen Hamilton , Huijuan Li , Natarajan Sethuraman , Terrance A. Stadheim , Stefan Wildt
发明人： Piotr Bobrowicz , Sujatha Gomathinayagam , Stephen Hamilton , Huijuan Li , Natarajan Sethuraman , Terrance A. Stadheim , Stefan Wildt
CPC分类号： C07K14/505 , C07K14/47 , C07K14/4715 , C07K14/4721 , C07K14/4743 , C07K14/485 , C07K14/52 , C07K14/523 , C07K14/535 , C07K14/54 , C07K14/555 , C07K14/59 , C07K14/605 , C07K14/705 , C07K14/70546 , C07K14/70578 , C07K14/7151 , C07K14/755 , C07K14/8114 , C07K14/8125 , C07K14/8128 , C07K16/18 , C07K16/22 , C07K2317/33 , C12N9/1051 , C12P21/005
摘要： Methods for producing proteins and glycoproteins in Pichia pastoris that lack detectable cross binding activity to antibodies made against host cell antigens are described. In particular, methods are described wherein recombinant Pichia pastoris strains that do not display a β-mannosyltransferase 2 activity with respect to an N-glycan or O-glycan and do not display at least one activity selected from a β-mannosyltransferase 1, 3, and 4 activity to produce recombinant proteins and glycoproteins. These recombinant Pichia pastoris strains can produce proteins and glycoproteins that lack detectable α-mannosidase resistant β-mannose residues thereon and thus, lack cross binding activity to antibodies against host cell antigens. Further described are methods for producing bi-sialylated human erythropoietin in Pichia pastoris that lack detectable cross binding activity to antibodies against host cell antigens.
摘要翻译： 描述了在针对宿主细胞抗原的抗体中缺乏可检测的交联结合活性的巴斯德毕赤酵母中产生蛋白质和糖蛋白的方法。 具体地说，描述了相对于N-聚糖或O-聚糖不显示“ - - 甘露糖基转移酶2”活性的重组毕赤酵母菌株并且不显示至少一种选自β-甘露糖基转移酶1， 3和4活性产生重组蛋白和糖蛋白。 这些重组毕赤酵母菌株可以产生在其上缺乏可检测的α-甘露糖苷酶抗性和甘露糖残基的蛋白质和糖蛋白，因此对抗宿主细胞抗原的抗体缺乏交叉结合活性。 进一步描述了在巴斯德毕赤酵母中生产双唾液酸化人促红细胞生成素的方法，其对抗宿主细胞抗原的抗体缺乏可检测的交联结合活性。
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5.发明授权Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins 有权在糖蛋白生产中消除聚糖的甘露糖基磷酸化的方法公开(公告)号：US07259007B2
公开(公告)日：2007-08-21
申请号：US11020808
申请日：2004-12-22
CPC分类号： C12N9/1051 , C07K14/39 , C12P21/005
摘要： The present invention relates to the elimination of mannosylphosphorylation on the glycans of glycoproteins in the yeast genus Pichia. The elimination of mannosylphosphorylated glycoproteins results from the disruption of the PNO1 gene and the newly isolated P. pastoris MNN4B gene. The present invention further relates to methods for producing modified glycan structures in host cells that are free of glycan mannosylphosphorylation.
摘要翻译： 本发明涉及消除酵母属毕赤酵母中糖蛋白聚糖上的甘露糖基磷酸化。 消除甘露糖基磷酸化糖蛋白是由PNO1基因和新分离的巴斯德毕赤酵母MNN4B基因的破坏产生的。 本发明还涉及在不含聚糖甘露糖基磷酸化的宿主细胞中产生修饰的聚糖结构的方法。
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6.发明申请YEAST STRAIN FOR THE PRODUCTION OF PROTEINS WITH TERMINAL ALPHA-1,3-LINKED GALACTOSE 审中-公开用终产的ALPHA-1,3连接胶质糖生产蛋白质的YEAST菌株公开(公告)号：US20110086054A1
公开(公告)日：2011-04-14
申请号：US12995284
申请日：2009-12-03
CPC分类号： C07K14/435 , C12N15/80 , C12P21/005
摘要： Lower eukaryotic host cells have been engineered to produce glycoprotein having at least one terminal α-galactosyl epitope. The glycoproteins are useful for the production of highly antigenic glycoprotein compositions with advantages for the production of vaccines.
摘要翻译： 已经将低等真核宿主细胞工程化以产生具有至少一个末端α-半乳糖基表位的糖蛋白。 糖蛋白可用于生产具有疫苗生产优点的高抗原性糖蛋白组合物。
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7.发明申请METHOD FOR PRODUCING PROTEINS IN PICHIA PASTORIS THAT LACK DETECTABLE CROSS BINDING ACTIVITY TO ANTIBODIES AGAINST HOST CELL ANTIGENS 审中-公开在PICHIA PASTORIS中生产蛋白质的方法，将检测到的交叉结合活性与抗宿主细胞抗原抗体公开(公告)号：US20140200180A1
公开(公告)日：2014-07-17
申请号：US14155878
申请日：2014-01-15
申请人： Piotri Bobrowicz , Sujatha Gomathinayagam , Stephen Hamilton , Huijuan Li , Natarajan Setheraman , Terrance A. Stadheim , Stephan Wildt
发明人： Piotri Bobrowicz , Sujatha Gomathinayagam , Stephen Hamilton , Huijuan Li , Natarajan Setheraman , Terrance A. Stadheim , Stephan Wildt
IPC分类号： C07K14/505 , C12P21/00 , C07K14/47
CPC分类号： C07K14/505 , C07K14/47 , C07K14/4715 , C07K14/4721 , C07K14/4743 , C07K14/485 , C07K14/52 , C07K14/523 , C07K14/535 , C07K14/54 , C07K14/555 , C07K14/59 , C07K14/605 , C07K14/705 , C07K14/70546 , C07K14/70578 , C07K14/7151 , C07K14/755 , C07K14/8114 , C07K14/8125 , C07K14/8128 , C07K16/18 , C07K16/22 , C07K2317/33 , C12N9/1051 , C12P21/005
摘要： Methods for producing proteins and glycoproteins in Pichia pastoris that lack detectable cross binding activity to antibodies made against host cell antigens are described. In particular, methods are described wherein recombinant Pichia pastoris strains that do not display a β-mannosyltransferase 2 activity with respect to an N-glycan or O-glycan and do not display at least one activity selected from a β-mannosyltransferase 1, 3, and 4 activity to produce recombinant proteins and glycoproteins. These recombinant Pichia pastoris strains can produce proteins and glycoproteins that lack detectable α-mannosidase resistant β-mannose residues thereon and thus, lack cross binding activity to antibodies against host cell antigens. Further described are methods for producing bi-sialylated human erythropoietin in Pichia pastoris that lack detectable cross binding activity to antibodies against host cell antigens.
摘要翻译： 描述了在针对宿主细胞抗原的抗体中缺乏可检测的交联结合活性的巴斯德毕赤酵母中产生蛋白质和糖蛋白的方法。 具体地说，描述了相对于N-聚糖或O-聚糖不显示“ - - 甘露糖基转移酶2”活性的重组毕赤酵母菌株并且不显示至少一种选自β-甘露糖基转移酶1， 3和4活性产生重组蛋白和糖蛋白。 这些重组毕赤酵母菌株可以产生在其上缺乏可检测的α-甘露糖苷酶抗性和甘露糖残基的蛋白质和糖蛋白，因此对抗宿主细胞抗原的抗体缺乏交叉结合活性。 进一步描述了在巴斯德毕赤酵母中生产双唾液酸化人促红细胞生成素的方法，其对抗宿主细胞抗原的抗体缺乏可检测的交联结合活性。
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8.发明授权Method for producing therapeutic proteins in Pichia pastoris lacking dipeptidyl aminopeptidase activity 有权不含二肽基氨基肽酶活性的巴斯德毕赤酵母中产生治疗性蛋白质的方法公开(公告)号：US09528116B2
公开(公告)日：2016-12-27
申请号：US13503663
申请日：2010-10-27
CPC分类号： C12N15/81 , C12N9/48 , C12P21/005 , C12P21/02 , Y02P20/52
摘要： The present invention related to methods and compositions for producing therapeutic proteins in yeast cell lines, and in particular Pichia pastoris, lacking dipeptidyl aminopeptidase (DAP) activity. DAP activity has been eliminated by genetically modifying a Pichia pastoris cell line such that STE13 and DAP2 have been deleted.
摘要翻译： 本发明涉及用于在酵母细胞系中产生治疗性蛋白质的方法和组合物，特别是缺乏二肽基氨基肽酶（DAP）活性的巴斯德毕赤酵母。 通过遗传修饰巴斯德毕赤酵母细胞系来消除DAP活性，使得STE13和DAP2被缺失。
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公开(公告)号：US09187552B2
公开(公告)日：2015-11-17
申请号：US13699213
申请日：2011-05-25
申请人： Terrance A. Stadheim , Dongxing Zha , Liming Liu
发明人： Terrance A. Stadheim , Dongxing Zha , Liming Liu
CPC分类号： C12P21/005 , A61K2039/505 , C07K16/00 , C07K16/241 , C07K16/2863 , C07K16/32 , C07K16/40 , C07K2317/14 , C07K2317/41 , C07K2317/51 , C07K2317/515 , C07K2317/52 , C07K2317/71 , C07K2317/732 , C07K2317/734
摘要： The present invention is directed to methods and compositions for the production of Fc-containing polypeptides having improved properties and comprising mutations at positions 243 and 264 of the Fc region.
摘要翻译： 本发明涉及用于生产具有改进性质并含有Fc区第243和264位突变的含Fc多肽的方法和组合物。
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公开(公告)号：US20130071390A1
公开(公告)日：2013-03-21
申请号：US13699213
申请日：2011-05-25
申请人： Terrance A. Stadheim , Dongxing Zha , Liming Liu
发明人： Terrance A. Stadheim , Dongxing Zha , Liming Liu
IPC分类号： C07K16/00
CPC分类号： C12P21/005 , A61K2039/505 , C07K16/00 , C07K16/241 , C07K16/2863 , C07K16/32 , C07K16/40 , C07K2317/14 , C07K2317/41 , C07K2317/51 , C07K2317/515 , C07K2317/52 , C07K2317/71 , C07K2317/732 , C07K2317/734
摘要： The present invention is directed to methods and compositions for the production of Fc-containing polypeptides having improved properties and comprising mutations at positions 243 and 264 of the Fc region.
摘要翻译： 本发明涉及用于生产具有改进性质并含有Fc区第243和264位突变的含Fc多肽的方法和组合物。
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