NUCLEIC ACID PURIFICATION METHOD
    43.
    发明申请
    NUCLEIC ACID PURIFICATION METHOD 有权
    核酸纯化方法

    公开(公告)号:US20080076912A1

    公开(公告)日:2008-03-27

    申请号:US11857882

    申请日:2007-09-19

    CPC classification number: C12N15/101

    Abstract: The invention provides an improved method for the purification of nucleic acid molecules, which method comprises generating a cellular lysate containing the nucleic acid; contacting the lysate with an anion exchanger bound to a solid support matrix under conditions such that the anion exchanger binds the nucleic acid; followed by eluting the nucleic acid from the anion exchanger with an aqueous mobile phase comprising an elution solution; and desalting the eluted nucleic acid such that it is suitable for downstream applications. The improvement of the method includes providing the anion exchanger in a packed column, wherein the column is packed using a salt solution containing an antimicrobial agent. In addition, the salt solution has a salt concentration similar to that of the lysate, such that the column does not need equilibration prior to sample loading.

    Abstract translation: 本发明提供了一种用于纯化核酸分子的改进方法,该方法包括产生含有核酸的细胞裂解物; 使所述裂解物与阴离子交换剂接触,所述阴离子交换剂在使所述阴离子交换剂结合所述核酸的条件下与固体支持基质结合; 随后用含有洗脱溶液的水性流动相从阴离子交换剂洗脱核酸; 并使洗脱的核酸脱盐,使其适合于下游应用。 该方法的改进包括在填充柱中提供阴离子交换剂,其中使用含有抗微生物剂的盐溶液填充柱。 此外,盐溶液的盐浓度与裂解物的盐浓度相似,使得柱在样品加载之前不需要平衡。

    Dual-band multiple beam antenna system for communication satellites

    公开(公告)号:US20060063528A1

    公开(公告)日:2006-03-23

    申请号:US11259913

    申请日:2005-10-27

    CPC classification number: H01Q13/0208 H01Q1/288 H01Q5/55 H01Q19/13 H01Q25/04

    Abstract: A dual-band multiple beam antenna system for a communications satellite sharing a set of reflector antennas for the transmit and receive frequencies. One set of reflectors is common to both the downlink and uplink frequencies. The feed horns are diplexed and exhibit frequency-dependent radiation patterns that separate the phase centers over the downlink and uplink frequency bands to obtain dual-band performance. The focal point of the reflector is in close proximity to the phase center corresponding to the downlink frequency band. The phase center for the uplink frequency band is spaced a predetermined distance from the phase center of the downlink frequency band. According to the present invention, the uplink frequencies are defocused and the downlink frequencies are focused thereby creating identical radiation patterns at both frequency bands and over the coverage region of the communications satellite.

    Antenna system for multiple orbits and multiple areas
    45.
    发明授权
    Antenna system for multiple orbits and multiple areas 有权
    用于多个轨道和多个区域的天线系统

    公开(公告)号:US06570528B1

    公开(公告)日:2003-05-27

    申请号:US10052971

    申请日:2001-11-09

    CPC classification number: H01Q15/147 H01Q1/288 H01Q25/00 Y10S343/02

    Abstract: A synthesized reflector surface (12) for directing communication signals (27) in a communication system (10) that operates in a plurality of orbital slots and to a plurality of regions (28) within a first coverage area (30) is provided. The synthesized reflector surface (12) includes a plurality of contiguous profile surfaces (40) that form the reflector surface (12). Each of the plurality of contiguous profile surfaces (40) alters the phase-of the communication signals (27) to provide a first gain for a first satellite orbit location (32) and a second gain for a second satellite orbit location (34). The plurality of contiguous profile surfaces (40) directs the signals from the location (32) in a first orientation to the first coverage area (30) or from the location (34) in a second orientation to the first coverage area (30). A method is provided for synthesizing the reflector surface (12). A satellite system (10) and a method of configuring the satellite system (10) are also provided utilizing the synthesized reflector surface (12).

    Abstract translation: 提供了一种用于在通信系统(10)中引导通信信号(12)的合成反射器表面(12),其在多个轨道时隙中工作,并且在第一覆盖区域(30)内的多个区域(28)上操作。 合成的反射器表面(12)包括形成反射器表面(12)的多个邻接的轮廓表面(40)。 多个连续轮廓表面(40)中的每一个改变通信信号(27)的相位,以为第一卫星轨道位置(32)提供第一增益,并为第二卫星轨道位置(34)提供第二增益。 多个相邻轮廓表面(40)将来自位置(32)的信号以第一取向引导到第一覆盖区域(30)或从第二取向的位置(34)引导到第一覆盖区域(30)。 提供了一种用于合成反射器表面(12)的方法。 利用合成的反射器表面(12)还提供卫星系统(10)和配置卫星系统(10)的方法。

    Reconfigurable antenna system
    47.
    发明授权
    Reconfigurable antenna system 失效
    可重构天线系统

    公开(公告)号:US06456251B1

    公开(公告)日:2002-09-24

    申请号:US09859546

    申请日:2001-05-17

    Applicant: Sudhakar Rao

    Inventor: Sudhakar Rao

    CPC classification number: H01Q19/12 H01Q1/288 H01Q3/02 H01Q3/20 H04B7/18515

    Abstract: A two-step method is used to reconfigure beams among different orbital slots for a satellite system. The satellite is biased at one or more orbital slots for a back-up mission, while it looks at the sub-satellite point for the primary mission. Body steering is achieved using pitch and roll biases. The main reflector of the antenna is gimballed in order to steer the beams to different locations for the back-up mission. Beam size is controlled through the use of different sized reflectors and by varying the feed size. Different sized beams are interleaved among multiple reflectors.

    Abstract translation: 使用两步法重新配置卫星系统的不同轨道时隙之间的波束。 卫星偏向于一个或多个轨道槽,用于后勤任务,同时它观察主要任务的副卫星点。 使用俯仰和倾斜偏压实现车身转向。 天线的主要反射器是gimballed,以便将梁转向不同的位置进行后备任务。 通过使用不同尺寸的反射器和改变进料尺寸来控制光束尺寸。 不同尺寸的光束在多个反射器之间交错。

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