公开(公告)号：US20210254054A1

公开(公告)日：2021-08-19

申请号：US17236660

申请日：2021-04-21

Applicant: TOOLGEN INCORPORATED ,  SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION ,  SEOUL NATIONAL UNIVERSITY HOSPITAL

Inventor： Jeong Hun KIM ,  Sung Wook PARK ,  Seokjoong KIM ,  Dong Woo SONG

IPC: C12N15/11 ,  C12N9/22 ,  A61K48/00 ,  C12N15/113 ,  C07K14/47 ,  C07K14/52 ,  C07K14/515 ,  C12N15/86 ,  A61K9/00 ,  A61K38/46

Abstract: The present invention relates to an artificially manipulated neovascularization-associated factor for regulating neovascularization and a use thereof. More particularly, the present invention relates to a system for artificially regulating neovascularization, which includes an artificially manipulated neovascularization-associated factor for regulating neovascularization and/or a composition for artificially manipulating the neovascularization-associated factor. In a specific aspect, a neovascularization regulatory system including a neovascularization-associated factor such as artificially manipulated VEGFA, HIF1A, ANGPT2, EPAS1, or ANGPTL4 and/or an expression product thereof is provided.

公开(公告)号：US20210047648A1

公开(公告)日：2021-02-18

申请号：US17004355

申请日：2020-08-27

Applicant: TOOLGEN INCORPORATED

Inventor： Jin-Soo Kim ,  Seung Woo Cho ,  Sojung Kim

IPC: C12N15/52 ,  C12N15/85 ,  C12N15/90 ,  C12N9/22 ,  C12N15/82 ,  C12N15/10 ,  C12N15/63 ,  C12N15/11 ,  C12N9/16

Abstract: The present invention relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present invention relates to a composition for cleaving a target DNA in eukaryotic cells or organisms comprising a guide RNA specific for the target DNA and Cas protein-encoding nucleic acid or Cas protein, and use thereof.

公开(公告)号：US20210047647A1

公开(公告)日：2021-02-18

申请号：US17004338

申请日：2020-08-27

Applicant: TOOLGEN INCORPORATED

Inventor： Jin-Soo Kim ,  Seung Woo Cho ,  Sojung Kim

IPC: C12N15/52 ,  C12N15/85 ,  C12N15/90 ,  C12N9/22 ,  C12N15/82 ,  C12N15/10 ,  C12N15/63 ,  C12N15/11 ,  C12N9/16

Abstract: The present invention relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present invention relates to a composition for cleaving a target DNA in eukaryotic cells or organisms comprising a guide RNA specific for the target DNA and Cas protein-encoding nucleic acid or Cas protein, and use thereof.

公开(公告)号：US10851380B2

公开(公告)日：2020-12-01

申请号：US14685568

申请日：2015-04-13

Applicant: TOOLGEN INCORPORATED

Inventor： Jin-Soo Kim ,  Seung Woo Cho ,  Sojung Kim

IPC: C12N15/85 ,  C12N15/52 ,  C12N15/90 ,  C12N9/22 ,  C12N15/82 ,  C12N15/10 ,  C12N15/63 ,  C12N15/11 ,  C12N9/16

Abstract: The present invention relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present invention relates to a composition for cleaving a target DNA in eukaryotic cells or organisms comprising a guide RNA specific for the target DNA and Cas protein-encoding nucleic acid or Cas protein, and use thereof.

公开(公告)号：US10519454B2

公开(公告)日：2019-12-31

申请号：US15420936

申请日：2017-01-31

Applicant: TOOLGEN INCORPORATED

Inventor： Eun Ji Kim ,  Seok Joong Kim

IPC: C12N15/63 ,  C12N9/16 ,  C12N7/00 ,  C12N15/86

Abstract: The disclosure provided herewith relates to a Campylobacter jejuni CRISPR/CAS system-derived RGEN and a use thereof.

公开(公告)号：US20180237771A1

公开(公告)日：2018-08-23

申请号：US15953141

申请日：2018-04-13

Applicant: TOOLGEN INCORPORATED ,  SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION ,  SEOUL NATIONAL UNIVERSITY HOSPITAL

Inventor： Jeong Hun KIM ,  Sung Wook PARK ,  Seokjoong KIM ,  Dong Woo SONG

IPC: C12N15/11 ,  C12N9/22 ,  C12N15/86 ,  A61K38/46 ,  A61K9/00

Abstract: The present invention relates to an artificially manipulated neovascularization-associated factor for regulating neovascularization and a use thereof. More particularly, the present invention relates to a system for artificially regulating neovascularization, which includes an artificially manipulated neovascularization-associated factor for regulating neovascularization and/or a composition for artificially manipulating the neovascularization-associated factor. In a specific aspect, a neovascularization regulatory system including a neovascularization-associated factor such as artificially manipulated VEGFA, HIF1A, ANGPT2, EPAS1, or ANGPTL4 and/or an expression product thereof is provided.

公开(公告)号：US20170191123A1

公开(公告)日：2017-07-06

申请号：US15314288

申请日：2015-05-28

Applicant: TOOLGEN INCORPORATED ,  INSTITUTE FOR BASIC SCIENCE

Inventor： Jin Soo Kim ,  So Jung Kim ,  Seung Hwan Lee ,  Seok Joong Kim

IPC: C12Q1/68

CPC classification number: C12Q1/6844 ,  C12Q1/68 ,  C12Q2521/301 ,  G01N33/53 ,  G01N33/574

Abstract: The present invention relates to a method for analyzing a genotype using a target-specific nuclease and, specifically, to a method for diagnosing cancer or analyzing a genotype by removing wild type DNA or particular genotype DNA using a target-specific nuclease or a variant thereof to amplify or concentrate only a small amount of DNA which has a difference in variation, such as a mutation, or genotype, and to a method for separating target DNA sesusing a target-specific nuclease or a variant thereof. Such methods are novel paradigm methods contrary to existing simple target-specific nucleases for post-PCR recognition of normal genotype and carcinogenic genotype, and can be favorably used in the early diagnosis of cancer or analysis of similar genotypes.

公开(公告)号：US20150322457A1

公开(公告)日：2015-11-12

申请号：US14685568

申请日：2015-04-13

Applicant: Toolgen Incorporated

Inventor： Jin-Soo Kim ,  Seung Woo Cho ,  Sojung Kim

IPC: C12N15/85 ,  C12N9/22

CPC classification number: C12N15/52 ,  C12N9/16 ,  C12N9/22 ,  C12N15/102 ,  C12N15/111 ,  C12N15/63 ,  C12N15/8216 ,  C12N15/85 ,  C12N15/907 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/531 ,  C12Y301/21

Abstract: The present invention relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present invention relates to a composition for cleaving a target DNA in eukaryotic cells or organisms comprising a guide RNA specific for the target DNA and Cas protein-encoding nucleic acid or Cas protein, and use thereof.

公开(公告)号：US20250154479A1

公开(公告)日：2025-05-15

申请号：US18832216

申请日：2023-01-20

Applicant: TOOLGEN INCORPORATED

Inventor： Younghoon KIM ,  Keunwoo CHOI ,  Jeongjoon LEE

IPC: C12N9/22 ,  C12N15/11

Abstract: The present disclosure discloses a SpCas9 variant. The SpCas9 variant is characterized by the ability to recognize a PAM sequence different from that of a wild-type SpCas9 protein.

公开(公告)号：US20250146003A1

公开(公告)日：2025-05-08

申请号：US19015836

申请日：2025-01-10

Applicant: TOOLGEN INCORPORATED

Inventor： Jin-Soo Kim ,  Seung Woo Cho ,  Sojung Kim

IPC: C12N15/52 ,  C12N9/16 ,  C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12N15/63 ,  C12N15/82 ,  C12N15/85 ,  C12N15/90

Abstract: The present disclosure relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present disclosure provides for compositions and methods that may induce modifications in target endogenous nucleic acid sequences in nucleuses of eukaryotic cells. For example, disclosed herein is a system for inducing targeted disruption of endogenous genes in a eukaryotic cell, the system comprising: an extracellular Cas9/RNA complex; cell-free and a buffer; wherein the extracellular Cas9/RNA complex comprises: a recombinant Cas9; and a guide RNA having a CRISPR RNA (crRNA) and a transactivating crRNA (tracrRNA); wherein the extracellular Cas9/RNA complex is disposed in the cell-free buffer and wherein the extracellular Cas9/RNA complex is complexed prior to being introduced into the eukaryotic cell; and wherein the Cas9/RNA complex functions as an endonuclease that induces targeted disruption of the target DNA upon introduction into the eukaryotic cell.