公开(公告)号：US12286637B2

公开(公告)日：2025-04-29

申请号：US18537046

申请日：2023-12-12

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Dongmei Xu ,  Jesse Frederick ,  Chengalrayan Kudithipudi ,  Yanxin Shen ,  James Strickland ,  Jaemo Yang

IPC: C12N15/82 ,  C07K14/415

Abstract: The present disclosure provides the identification of genes involved in sucker growth in tobacco. Also provided are promoters that are preferentially active in tobacco axillary buds. Also provided are modified tobacco plants comprising reduced or no sucker growth. Also provided are methods and compositions for producing modified tobacco plants comprising reduced or no sucker growth.

公开(公告)号：US20250129379A1

公开(公告)日：2025-04-24

申请号：US18985344

申请日：2024-12-18

Applicant: Altria Client Services LLC

Inventor： Dongmei Xu ,  Chengalrayan Kudithipudi ,  Yanxin Shen ,  Jaemo Yang ,  Jesse Frederick ,  James Strickland

IPC: C12N15/82 ,  A24B13/00 ,  A24B15/10 ,  C07K14/415

Abstract: The present disclosure provides the identification of genes involved in sucker growth in tobacco. Also provided are promoters that are preferentially active in tobacco axillary buds. Also provided are modified tobacco plants comprising reduced or no sucker growth. Also provided are methods and compositions for producing modified tobacco plants comprising reduced or no sucker growth.

公开(公告)号：US12209249B2

公开(公告)日：2025-01-28

申请号：US18476495

申请日：2023-09-28

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Dongmei Xu ,  Chengalrayan Kudithipudi ,  Yanxin Shen ,  Jaemo Yang ,  Jesse Frederick ,  James Strickland

IPC: C12N15/82 ,  A24B13/00 ,  A24B15/10 ,  C07K14/415

Abstract: The present disclosure provides the identification of genes involved in sucker growth in tobacco. Also provided are promoters that are preferentially active in tobacco axillary buds. Also provided are modified tobacco plants comprising reduced or no sucker growth. Also provided are methods and compositions for producing modified tobacco plants comprising reduced or no sucker growth.

公开(公告)号：US12077765B2

公开(公告)日：2024-09-03

申请号：US16750349

申请日：2020-01-23

Applicant: Altria Client Services LLC

Inventor： Chengalrayan Kudithipudi ,  Dongmei Xu ,  James A. Strickland ,  Marcos Fernando de Godoy Lusso

IPC: C12N15/82

CPC classification number: C12N15/8243

Abstract: The present disclosure provides approaches for reducing nicotine. Also provided are tobacco plants with decreased tobacco-specific nitrosamines (TSNAs). The present disclosure further provides modified tobacco plants with low nicotine and increased antioxidant capacity. Further provided are tobacco plants or material with low nicotine and low TSNAs. Also provided is cured tobacco material of the tobacco plants provided herein and tobacco products comprising this cured tobacco material.

公开(公告)号：US11602118B2

公开(公告)日：2023-03-14

申请号：US17116816

申请日：2020-12-09

Applicant: Altria Client Services LLC

Inventor： Jesse Frederick ,  Chengalrayan Kudithipudi ,  Dongmei Xu

IPC: C12N15/82 ,  A01H5/12 ,  A01H6/82 ,  C07K14/415 ,  C12N9/90 ,  C12N9/10 ,  C12N9/88 ,  A01H5/10

Abstract: The present disclosure provides metabolic signatures and genetic markers for tracking enhanced nitrogen utilization efficiency phenotypes in tobacco plants and for introgressing enhanced nitrogen utilization efficiency phenotypes into tobacco plants. The disclosure also provides tobacco plants comprising enhanced nitrogen utilization efficiency and methods to the creation of tobacco plants comprising enhanced nitrogen utilization efficiency. The disclosure also provides recombinant polynucleotides and polypeptides for enhancing nitrogen utilization efficiency in modified tobacco plants and tobacco plants comprising the provided recombinant polynucleotides and polypeptides.

公开(公告)号：US11484002B2

公开(公告)日：2022-11-01

申请号：US16529307

申请日：2019-08-01

Applicant: Altria Client Services LLC

Inventor： Chengalrayan Kudithipudi ,  Rutger Van Der Hoeven ,  Ndjido Ardo Kane

IPC: A01H5/12 ,  A24B13/00 ,  A24D1/00 ,  A01H6/82 ,  A01H1/06

Abstract: A process for producing a reduced tobacco-specific nitrosamine (TSNA) tobacco plant comprising reducing and/or eliminating anatabine biosynthesis in wild-type tobacco plant. In addition, use of such plants for discovery of molecular markers that are closely linked with genes required for anatabine biosynthesis and for discovery of genes required for anatabine biosynthesis. In addition, a smoking composition, a smoking article and a smokeless tobacco oral delivery product contain the tobacco material.

公开(公告)号：US11447790B2

公开(公告)日：2022-09-20

申请号：US16886118

申请日：2020-05-28

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Raja S. Payyavula ,  Yanxin Shen ,  Chengalrayan Kudithipudi ,  Rajanikanth Govindarajulu

IPC: C12N15/82

Abstract: The present disclosure provides methods and compositions for controlling sucker growth in tobacco by altering the expression of different target genes.

公开(公告)号：US11384361B2

公开(公告)日：2022-07-12

申请号：US16523326

申请日：2019-07-26

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Chengalrayan Kudithipudi ,  Dong Qi ,  Yanxin Shen ,  Ujwala Warek ,  James A. Strickland ,  Dongmei Xu

IPC: A01H5/12 ,  C12N15/00 ,  C12N15/82 ,  A01H6/82 ,  C12Q1/6827

Abstract: The present disclosure provides compositions and methods related to tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants.

公开(公告)号：US11377665B2

公开(公告)日：2022-07-05

申请号：US16372833

申请日：2019-04-02

Applicant: Altria Client Services LLC

Inventor： Chengalrayan Kudithipudi ,  Dongmei Xu ,  James Strickland ,  Yanxin Shen

IPC: C12N15/82 ,  A24B15/10 ,  C12N9/88 ,  A24B13/00 ,  A24B15/24 ,  A24B15/20

Abstract: The present disclosure provides approaches for reducing tobacco-specific nitrosamines (TSNAs) in tobacco. Some of these approaches include genetically engineering tobacco plants to increase one or more antioxidants, increase oxygen radicle absorbance capacity (ORAC), increase phenylalanine, or reduce nitrite. Also provided are methods and compositions for producing modified tobacco plants and tobacco products therefrom comprising reduced TSNAs. Also provided are methods and compositions for increasing the expression of chorismate mutase and other transcription factors involved in anthocyanin biosynthesis.

公开(公告)号：US11293030B2

公开(公告)日：2022-04-05

申请号：US16529365

申请日：2019-08-01

Applicant: Altria Client Services LLC

Inventor： Chengalrayan Kudithipudi ,  Jaemo Yang ,  Yanxin Shen ,  Dongmei Xu ,  Ujwala Warek ,  James Strickland ,  Jesse Frederick

IPC: C12N15/82

Abstract: The present disclosure provides the identification of promoters that are preferentially active in tobacco axillary buds. Also provided are tobacco plants comprising reduced or no sucker growth. Also provided are methods and compositions for producing tobacco plants comprising reduced or no sucker growth.