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公开(公告)号：US06204232B1
公开(公告)日：2001-03-20
申请号：US09183412
申请日：1998-10-30
申请人： Torben Vedel Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Lauesgaard Nissen , Søren Kjærulff
发明人： Torben Vedel Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Lauesgaard Nissen , Søren Kjærulff
IPC分类号： C12N928
CPC分类号： C12N9/2417 , C07K14/32 , C11D3/386 , C11D3/38609 , C11D3/38636 , C12Y302/01001
摘要： The invention relates to a variant of a parent Termamyl-like &agr;-amylase, which exhibits an alteration in at least one of the following properties relative to said parent &agr;-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.
摘要翻译： 本发明涉及亲本的类戊酰样α-淀粉酶的变体，其相对于所述亲本α-淀粉酶表现出至少一种以下特性的改变：i）在8至10.5的pH下改善的pH稳定性; 和/或ii）在pH8至10.5时提高的Ca 2+稳定性，和/或iii）在10至60℃的温度下提高比活性。
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公开(公告)号：US08252573B2
公开(公告)日：2012-08-28
申请号：US13020545
申请日：2011-02-03
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38618 , C12P7/06 , C12P7/14 , C12P19/14 , C12Y302/01001 , Y02E50/17
摘要： The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.
摘要翻译： 本发明涉及亲本Termamyl样α-淀粉酶的变体（突变体），该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶具有改变的性质。
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公开(公告)号：US20110159570A1
公开(公告)日：2011-06-30
申请号：US13010219
申请日：2011-01-20
IPC分类号： C12N9/28
CPC分类号： C12N9/2417 , A21D2/26 , A21D8/042
摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.
摘要翻译： 基于麦芽糖α-淀粉酶Novamyl的三维结构，本发明人已经修饰了产麦芽糖α-淀粉酶的氨基酸序列以获得具有改进性质的变体。 这些变体具有改变的物理化学性质，例如 改变的pH最佳，改善的热稳定性，增加的比活性，改变的切割模式或增加降低淀粉退化或面包陈化的能力。
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公开(公告)号：US20090263881A1
公开(公告)日：2009-10-22
申请号：US11953532
申请日：2007-12-10
申请人： Torben Vedel Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Lauesgaard Nissen , Soren Kjaerulff
发明人： Torben Vedel Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Lauesgaard Nissen , Soren Kjaerulff
IPC分类号： C12N9/26
摘要： The invention relates to a variant of a parent Termamyl-like α-amylase, which exhibits an alteration in at least one of the following properties relative to said parent α-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.
摘要翻译： 本发明涉及亲本的类戊酰样α-淀粉酶的变体，其相对于所述亲本α-淀粉酶表现出至少一种以下特性的改变：i）在8至10.5的pH下改善的pH稳定性; 和/或ii）在pH8至10.5时提高的Ca 2+稳定性，和/或iii）在10至60℃的温度下提高比活性。
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公开(公告)号：US20090155882A1
公开(公告)日：2009-06-18
申请号：US11934136
申请日：2007-11-02
申请人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
发明人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
CPC分类号： C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
摘要翻译： 本发明涉及母体类戊酰样α-淀粉酶的变体，该变体表现出改变的性质，特别是降低了切割接近分支点的底物的能力，以及相对于母体改善的底物特异性和/或改善的比活性 α-淀粉酶。
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公开(公告)号：US20090143270A1
公开(公告)日：2009-06-04
申请号：US12360635
申请日：2009-01-27
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38618 , C12P7/06 , C12P7/14 , C12P19/14 , C12Y302/01001 , Y02E50/17
摘要： The present invention relates to variants (mutants) of parent Termamyl-tike alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.
摘要翻译： 本发明涉及亲本Termamyl-tikeα-淀粉酶的变体（突变体），该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶具有改变的性质。
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公开(公告)号：US08486681B2
公开(公告)日：2013-07-16
申请号：US12360635
申请日：2009-01-27
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38618 , C12P7/06 , C12P7/14 , C12P19/14 , C12Y302/01001 , Y02E50/17
摘要： The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.
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48.发明申请CLEANING COMPOSITIONS COMPRISING AMYLASE VARIANTS REFERENCE TO A SEQUENCE LISTING 有权包含氨基酸变体的清洁组合物参考序列表公开(公告)号：US20130000055A1
公开(公告)日：2013-01-03
申请号：US13537606
申请日：2012-06-29
申请人： Michelle Jackson , Philip Frank Souter , Lindsay Suzanne Bewick , Svend Kaasgaard , Jens Oebro , Signe Eskildsen Larsen , Allan Svendsen , Annette Helle Johansen , Michael Skjoet , Carsten Andersen , Lars Beier , Esben Peter Friis , Miguel Duarte Guilherme Pereira Toscano , Mads Bjoernvad , Frank Winther Rasmussen , Liv Spaangner Christiansen
发明人： Michelle Jackson , Philip Frank Souter , Lindsay Suzanne Bewick , Svend Kaasgaard , Jens Oebro , Signe Eskildsen Larsen , Allan Svendsen , Annette Helle Johansen , Michael Skjoet , Carsten Andersen , Lars Beier , Esben Peter Friis , Miguel Duarte Guilherme Pereira Toscano , Mads Bjoernvad , Frank Winther Rasmussen , Liv Spaangner Christiansen
CPC分类号： C11D3/386 , C11D3/38681 , C12N9/2417 , C12Y302/01001
摘要： The present invention relates to cleaning compositions comprising variants of an alpha-amylase and methods of treating surfaces such as textiles with aqueous liquor comprising such compositions, especially at low temperatures.
摘要翻译： 本发明涉及包含α-淀粉酶的变体的清洁组合物以及包含这种组合物的含水液体的表面如纺织品的处理方法，特别是在低温下。
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公开(公告)号：US20120270253A1
公开(公告)日：2012-10-25
申请号：US13533055
申请日：2012-06-26
CPC分类号： C12N9/2417 , A21D2/26 , A21D8/042
摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties., e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.
摘要翻译： 基于麦芽糖α-淀粉酶Novamyl的三维结构，本发明人已经修饰了产麦芽糖α-淀粉酶的氨基酸序列以获得具有改进性质的变体。 这些变体具有改变的物理化学性质。 改变的pH最佳，改善的热稳定性，增加的比活性，改变的切割模式或增加降低淀粉退化或面包陈化的能力。
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公开(公告)号：US08232086B2
公开(公告)日：2012-07-31
申请号：US13010219
申请日：2011-01-20
CPC分类号： C12N9/2417 , A21D2/26 , A21D8/042
摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.
摘要翻译： 基于麦芽糖α-淀粉酶Novamyl的三维结构，本发明人已经修饰了产麦芽糖α-淀粉酶的氨基酸序列以获得具有改进性质的变体。 这些变体具有改变的物理化学性质，例如 改变的pH最佳，改善的热稳定性，增加的比活性，改变的切割模式或增加降低淀粉退化或面包陈化的能力。
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