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    Alpha-amylase mutants
    8.
    发明授权
    Alpha-amylase mutants 失效
    α-淀粉酶突变体

    公开(公告)号：US08323946B2

    公开(公告)日：2012-12-04

    申请号：US13161931

    申请日：2011-06-16

    申请人： Allan Svendsen ,  Henrik Bisgard-Frantzen ,  Torben Vedel Borchert

    发明人： Allan Svendsen ,  Henrik Bisgard-Frantzen ,  Torben Vedel Borchert

    IPC分类号： C12N9/00 ,  C12N9/30 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C11D3/386 ,  C12N9/2417

    摘要： The present invention relates to a method of constructing a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and at least one altered property as compared to the parent alpha-amylase, comprises i) analyzing the structure of the parent Termamyl-like alpha-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like alpha-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like alpha-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like alpha-amylase variant, which as compared to the parent Termamyl-like alpha-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like alpha-amylase variant with respect to the property in question.

    摘要翻译： 本发明涉及一种构建母体类戊酰样α-淀粉酶的变体的方法，该变体具有与母体α-淀粉酶相比具有α-淀粉酶活性和至少一种改变的性质，包括：i）分析 鉴定至少一个氨基酸残基或类甲酰样α-淀粉酶结构的至少一个结构部分，所述氨基酸残基或结构部分被认为与改变其性质有关的相关性 母体类戊酰样α-淀粉酶（根据结构或功能考虑进行评估），ii）构建与母体类戊酰样α-淀粉酶相比的类戊酰样α-淀粉酶变体，已经在 在i）中鉴定的氨基酸残基或结构部分，以改变其性质，以及任选地，iii）相对于所讨论的性质测试所得的类戊酰样α-淀粉酶变体。

    Alpha-amylase mutants
    9.
    发明授权
    Alpha-amylase mutants 有权
    α-淀粉酶突变体

    公开(公告)号：US08263368B2

    公开(公告)日：2012-09-11

    申请号：US12493860

    申请日：2009-06-29

    申请人： Allan Svendsen ,  Torben Vedel Borchert ,  Henrik Bisgard-Frantzen ,  Helle Outtrup ,  Bjarne Ronfeldt Nielsen ,  Vibeke Skovgaard Nielsen ,  Lisbeth Hedegaard

    发明人： Allan Svendsen ,  Torben Vedel Borchert ,  Henrik Bisgard-Frantzen ,  Helle Outtrup ,  Bjarne Ronfeldt Nielsen ,  Vibeke Skovgaard Nielsen ,  Lisbeth Hedegaard

    IPC分类号： C12N9/28 ,  C12S11/00 ,  C12P19/14

    CPC分类号： C12N9/2417 ,  C11D3/386 ,  C11D3/38609 ,  C11D3/38618 ,  C12Y302/01001

    摘要： The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).

    摘要翻译： 本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。 这些变体在酸性pH和/或低Ca 2+浓度（相对于亲本）下具有增加的热稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体在酸性pH和/或低Ca 2+浓度（相对于亲本）中表现出增加的热稳定性。

    Alpha-amylase mutants
    10.
    发明授权
    Alpha-amylase mutants 有权
    α-淀粉酶突变体

    公开(公告)号：US08263382B2

    公开(公告)日：2012-09-11

    申请号：US13100407

    申请日：2011-05-04

    申请人： Allan Svendsen ,  Torben Vedel Borchert ,  Henrik Bisgard-Frantzen

    发明人： Allan Svendsen ,  Torben Vedel Borchert ,  Henrik Bisgard-Frantzen

    IPC分类号： C12N9/24

    CPC分类号： C12N9/2417 ,  C11D3/386 ,  C12P19/02 ,  C12P19/14

    摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to the parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.

    摘要翻译： 本发明涉及母体类戊酰样α-淀粉酶的变体，该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶在下列特性中表现出至少一个变化：底物特异性，底物结合，底物 裂解模式，热稳定性，pH /活性分布，pH /稳定性分布，对氧化的稳定性，Ca 2+依赖性和比活性。