公开(公告)号：US20080095800A1

公开(公告)日：2008-04-24

申请号：US12001920

申请日：2007-12-13

申请人： Michael Houghton ,  Mark Selby ,  Sergio Abrignani ,  Jens Martin Heile ,  Derek O'Hagan

发明人： Michael Houghton ,  Mark Selby ,  Sergio Abrignani ,  Jens Martin Heile ,  Derek O'Hagan

IPC分类号： A61K39/00 ,  A61P1/16

CPC分类号： C07K14/005 ,  A61K9/167 ,  A61K31/7088 ,  A61K39/12 ,  A61K39/29 ,  A61K2039/53 ,  A61K2039/55511 ,  A61K2039/55555 ,  A61K2039/55566 ,  A61K2039/57 ,  C12N2770/24222 ,  C12N2770/24234

摘要： Described herein is a method of eliciting antibodies and neutralizing of binding antibodies against a hepatitis C virus (HCV) E1E2 or E2 antigen using HCV E2 or HCV E1E2 polypeptides and/or HCV E2 or E1E2 polynucleotides. Elicitation of anti-E2 antibodies and anti-E2 NOB antibodies can be used, inter alia, to provide model systems to optimize anti-E2 antibody responses and/or anti-E2 NOB antibody responses to HCV and to provide prophylactic or therapeutic treatment against HCV infection.

公开(公告)号：US07183107B2

公开(公告)日：2007-02-27

申请号：US10684846

申请日：2003-10-14

申请人： Mark Selby ,  Hui-Hua Lu

发明人： Mark Selby ,  Hui-Hua Lu

IPC分类号： C12N5/10 ,  C12N5/22 ,  C12N7/08 ,  C12N1/36 ,  A61K39/29

CPC分类号： C12N9/1205 ,  C12N7/00 ,  C12N15/69 ,  C12N15/86 ,  C12N2770/24011 ,  C12N2840/203

摘要： This invention provides methods of generating cells that stably replicate sub-genomic virus replicons. This invention also provides methods of generating cells that have disabled PKR activity and that stably replicate HCV sub-genomic replicons. The invention also provides methods of using the cells of the invention to screen for compounds that modulate viral RNA replication, including HCV RNA replication.

摘要翻译： 本发明提供了产生稳定复制亚基因组病毒复制子的细胞的方法。 本发明还提供了产生具有阻止PKR活性并稳定复制HCV亚基因组复制子的细胞的方法。 本发明还提供了使用本发明的细胞筛选调节病毒RNA复制（包括HCV RNA复制）的化合物的方法。

公开(公告)号：US06660471B2

公开(公告)日：2003-12-09

申请号：US09922962

申请日：2001-08-03

申请人： Mark Selby ,  Hui-Hua Lu

发明人： Mark Selby ,  Hui-Hua Lu

IPC分类号： C12Q100

CPC分类号： C12N9/1205 ,  C12N7/00 ,  C12N15/69 ,  C12N15/86 ,  C12N2770/24011 ,  C12N2840/203

摘要： This invention provides methods of generating cells that stably replicate sub-genomic virus replicons. This invention also provides methods of generating cells that have disabled PKR activity and that stably replicate HCV sub-genomic replicons. The invention also provides methods of using the cells of the invention to screen for compounds that modulate viral RNA replication, including HCV RNA replication.

公开(公告)号：US06326171B1

公开(公告)日：2001-12-04

申请号：US09415582

申请日：1999-10-08

申请人： Mark Selby ,  Michael Houghton

发明人： Mark Selby ,  Michael Houghton

IPC分类号： C12N1509

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2770/24222 ,  Y10S530/826

摘要： Novel Hepatitis C E1 and E2 truncated polypeptides and complexes comprising these polypeptides, are disclosed. The polypeptides are C-terminally truncated to remove all or a portion of their membrane spanning domains. Hence, the polypeptides are capable of secretion when expressed recombinantly.

摘要翻译： 公开了新型丙型肝炎E1和E2截短的多肽和包含这些多肽的复合物。 多肽被C-末端截短以除去其跨膜结构域的全部或部分。 因此，当重组表达时，多肽能够分泌。