公开(公告)号：US20180094273A1

公开(公告)日：2018-04-05

申请号：US15698702

申请日：2017-09-08

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Pierluigi Barone ,  Daren Hemingway ,  Emily Etchison ,  Andrew Asberry ,  Heather Pence ,  Andrew J. Bowling

IPC: C12N15/82

CPC classification number: C12N15/8234 ,  C12N15/8209 ,  C12N15/8233 ,  C12N15/8261

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Zea mays egg cell gene. Some embodiments relate to a promoter from a Zea mays egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR from a Zea mays egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.

公开(公告)号：US09914934B2

公开(公告)日：2018-03-13

申请号：US15097349

申请日：2016-04-13

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Daren Hemingway ,  Carla Ausmus ,  Andrew F. Worden ,  Andrew Asberry

IPC: C12N15/82 ,  C07K14/825

CPC classification number: C12N15/8227 ,  C07K14/825 ,  C12N15/8216

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Panicum virgatum metallothionein-like gene (mtl). Some embodiments relate to a promoter from a Panicum virgatum metallothionein-like gene (mtl) that functions in plants to promote transcription of operably linked nucleotide sequences.

公开(公告)号：US20170298373A1

公开(公告)日：2017-10-19

申请号：US15453121

申请日：2017-03-08

Applicant: Dow AgroSciences LLC

Inventor： Manju Gupta ,  Sandeep Kumar ,  Shavell Gorman ,  Andrew F. Worden

IPC: C12N15/82 ,  C12N15/65 ,  A01H5/12

CPC classification number: C12N15/8225 ,  C12N15/113 ,  C12N15/8223 ,  C12N15/8286 ,  Y02A40/162

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G138258 promoter. Some embodiments relate to a Zea mays GRMZM2G138258 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G138258 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.

公开(公告)号：US09777286B2

公开(公告)日：2017-10-03

申请号：US14505055

申请日：2014-10-02

Applicant: Dow AgroSciences LLC

Inventor： Manju Gupta ,  Sandeep Kumar ,  Navin Elango ,  Karthik Narayna Muthuraman ,  Jeffrey Beringer ,  Huixia Wu ,  Nagesh Sardesai

IPC: C12N15/82

CPC classification number: C12N15/8227 ,  C12N15/8216 ,  C12N15/8222

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays metallothionein-like gene regulatory elements.

公开(公告)号：US09758789B2

公开(公告)日：2017-09-12

申请号：US14472969

申请日：2014-08-29

Applicant: Dow AgroSciences, LLC

Inventor： Sandeep Kumar ,  Andrew Asberry

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8216 ,  C07K14/415

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using the regulatory elements, including the promoters and/or 3′-UTRs, isolated from Setaria italica ubiquitin genes.

公开(公告)号：US09650640B2

公开(公告)日：2017-05-16

申请号：US14472957

申请日：2014-08-29

Applicant: Dow AgroSciences, LLC

Inventor： Sandeep Kumar ,  Andrew F. Worden

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8216 ,  C07K14/415

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using the regulatory elements, including the promoters and/or 3′-UTRs, isolated from Panicum virgatum ubiquitin genes.

公开(公告)号：US09422569B2

公开(公告)日：2016-08-23

申请号：US13674606

申请日：2012-11-12

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Diaa Alabed ,  Terry Wright ,  Manju Gupta

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8222 ,  C12N15/8286 ,  Y02A40/162

Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bidirectional promoter link to multiple gene expression cassettes. In some embodiments, the constructs and methods provided employ a bidirectional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

Abstract translation: 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 所提供的构建物包含至少一个与多个基因表达盒的双向启动子连接。 在一些实施方案中，提供的构建体和方法使用基于来自玉米泛素-1基因的最小核心启动子元件或其功能等同物的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。

公开(公告)号：US20160130595A1

公开(公告)日：2016-05-12

申请号：US14938643

申请日：2015-11-11

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Toby Cicak ,  Heather Leigh Robinson ,  Dayakar Reddy Pareddy ,  Wei Chen

IPC: C12N15/82

CPC classification number: C12N15/8216

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a minimal core promoter element from a Arabidopsis thaliana Ubiquitin-10 gene promoter or Cassava Vein Mosaic Virus promoter, and the full-length nucleotide sequence elements from a Cassava Vein Mosaic Virus promoter. Some embodiments relate to a synthetic CsVMV bi-directional promoter that functions in plants to promote transcription of two operably linked nucleotide sequences.

Abstract translation: 本公开涉及用于促进植物或植物细胞中核苷酸序列的转录的组合物和方法，其使用来自拟南芥泛素-10基因启动子或木薯浆静脉花叶病毒启动子的最小核心启动子元件，以及全长核苷酸序列元件 从木薯静脉花叶病毒启动子。 一些实施方案涉及在植物中起作用以促进两个可操作连接的核苷酸序列的转录的合成的CsVMV双向启动子。

公开(公告)号：US20150184178A1

公开(公告)日：2015-07-02

申请号：US14577887

申请日：2014-12-19

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Marcelo Ariel German ,  Tristan Coram ,  Terry R. Wright

IPC: C12N15/82

Abstract: This disclosure concerns the use of endogenous plant RNAi machinery to preferentially or specifically reduce transgene expression. In some embodiments, the disclosure concerns specific reduction of transgene expression in male plant tissues, for example, to provide an economical male sterility system of hybrid seed production.

Abstract translation: 本公开涉及使用内源植物RNAi机制来优先或特异性地降低转基因表达。 在一些实施方案中，本公开涉及在雄性植物组织中特异性降低转基因表达，例如提供杂交种子生产的经济雄性不育系统。

公开(公告)号：US20140090113A1

公开(公告)日：2014-03-27

申请号：US14020575

申请日：2013-09-06

Applicant: Dow AgroSciences LLC

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Mike Ainley ,  Matthew J. Henry ,  John Mason ,  Sandeep Kumar ,  Stephen Novak

IPC: C12N15/82

Abstract: An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5′ and 3′ ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.

Abstract translation: 描述了可以随机或在植物基因组中的目标位置插入工程化转基因整合平台（ETIP），以便于在ETIP基因组位置上完全靶向（5'和3'末端）的GOI的快速选择和检测 。 主题披露中的一个要素是在ETIP内引入特定的双链断裂。 在一些实施方案中，使用锌指核酸酶结合位点描述ETIP，但可以利用其它靶向技术，例如大范围核酸酶，CRISPR，TAL或亮氨酸拉链。 还描述了转基因植物的组合物和生产方法，其中供体或有效载体DNA表达已经稳定整合到植物细胞中的ETIP中的外源核酸序列（例如蛋白质或RNA）的一种或多种产物。 在实施方案中，ETIP有助于从构想到开发阶段测试基因候选物和植物表达载体。