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    ENGINEERED TRANSGENE INTEGRATION PLATFORM (ETIP) FOR GENE TARGETING AND TRAIT STACKING
    9.
    发明申请
    ENGINEERED TRANSGENE INTEGRATION PLATFORM (ETIP) FOR GENE TARGETING AND TRAIT STACKING 审中-公开
    用于基因定位和路由堆栈的工程变换集成平台（ETIP）

    公开(公告)号：US20140090113A1

    公开(公告)日：2014-03-27

    申请号：US14020575

    申请日：2013-09-06

    Applicant: Dow AgroSciences LLC

    Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Mike Ainley ,  Matthew J. Henry ,  John Mason ,  Sandeep Kumar ,  Stephen Novak

    IPC: C12N15/82

    Abstract: An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5′ and 3′ ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.

    Abstract translation: 描述了可以随机或在植物基因组中的目标位置插入工程化转基因整合平台（ETIP），以便于在ETIP基因组位置上完全靶向（5'和3'末端）的GOI的快速选择和检测 。 主题披露中的一个要素是在ETIP内引入特定的双链断裂。 在一些实施方案中，使用锌指核酸酶结合位点描述ETIP，但可以利用其它靶向技术，例如大范围核酸酶，CRISPR，TAL或亮氨酸拉链。 还描述了转基因植物的组合物和生产方法，其中供体或有效载体DNA表达已经稳定整合到植物细胞中的ETIP中的外源核酸序列（例如蛋白质或RNA）的一种或多种产物。 在实施方案中，ETIP有助于从构想到开发阶段测试基因候选物和植物表达载体。

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