公开(公告)号：US20210040534A1

公开(公告)日：2021-02-11

申请号：US17070796

申请日：2020-10-14

Applicant: ELEMENT BIOSCIENCES, INC.

Inventor： Chunhong ZHOU ,  Sinan ARSLAN ,  Molly Min HE ,  Matthew KELLINGER ,  Adeline Huizhen MAH ,  Michael PREVITE ,  Lei SUN

IPC: C12Q1/682 ,  C12Q1/6869 ,  C12Q1/6867

Abstract: Methods and formulations for preparing low non-specific binding surfaces are described, and the prepared surface can provide improved performance for nucleic acid detection and base calling applications. The surface provides more accurate nucleic acid detection, enhanced contrast to noise ratio, and better data collection.

公开(公告)号：US20200248258A1

公开(公告)日：2020-08-06

申请号：US16855877

申请日：2020-04-22

Applicant: ELEMENT BIOSCIENCES, INC.

Inventor： Sinan ARSLAN ,  Chunhong ZHOU ,  Molly Min HE ,  Matthew KELLINGER ,  Adeline Huizhen MAH ,  Michael PREVITE ,  Lei SUN

IPC: C12Q1/6876 ,  G01N33/543 ,  C12N9/12 ,  G01N21/64

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US20250019760A1

公开(公告)日：2025-01-16

申请号：US18654808

申请日：2024-05-03

Applicant: Element Biosciences, Inc.

Inventor： William LIGHT ,  Hua YU ,  Junhua ZHAO ,  Su ZHANG ,  Samantha SNOW ,  Sinan ARSLAN ,  Matthew KELLINGER ,  Marco TJIOE ,  Scott IM ,  James GHADIALI ,  Michael KIM ,  Hermes TAYLOR ,  Michael PREVITE ,  Jake LEVIEUX ,  Ramreddy TIPANNA ,  Molly HE

IPC: C12Q1/6874 ,  C12Q1/44 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6876

Abstract: The present disclosure provides compositions comprising enzyme-based reagents, and methods using the enzyme-based reagents, for nucleic acid sequencing. The enzyme-based reagents efficiently remove sequencing read products from a first sequenced region of a template molecule, thereby reducing residual signals in a second sequenced region on the same template molecule.

公开(公告)号：US20240230631A1

公开(公告)日：2024-07-11

申请号：US18431748

申请日：2024-02-02

Applicant: Element Biosciences, Inc.

Inventor： Sinan Arslan ,  Molly HE ,  Matthew KELLINGER ,  Jake LEVIEUX ,  Michael PREVITE ,  Junhua ZHAO ,  Su ZHANG

IPC: G01N33/53 ,  C12Q1/6874 ,  G01N33/58

CPC classification number: G01N33/5308 ,  C12Q1/6874 ,  G01N33/582

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US20230296592A1

公开(公告)日：2023-09-21

申请号：US18202246

申请日：2023-05-25

Applicant: Element Biosciences, Inc.

Inventor： Michael PREVITE ,  Molly HE ,  Junhua ZHAO ,  Hui Zhen MAH ,  Chunhong ZHOU ,  Sinan ARSLAN ,  Matthew KELLINGER ,  Lorenzo BERTI ,  Steve Xiangling CHEN

IPC: G01N33/53 ,  C12Q1/6874 ,  G01N33/58

CPC classification number: G01N33/5308 ,  C12Q1/6874 ,  G01N33/582

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US20230038526A1

公开(公告)日：2023-02-09

申请号：US17695494

申请日：2022-03-15

Applicant: Element Biosciences, Inc.

Inventor： Sinan ARSLAN ,  Molly HE ,  Michael PREVITE

IPC: C12Q1/6834 ,  C12Q1/6832 ,  B01J19/00 ,  C12Q1/6806

Abstract: Nucleic acid hybridization buffer formulations and uses thereof are described that yield improvements in hybridization specificity, rate, and efficiency. The buffer formulation composition includes a target nucleic acid; at least one organic solvent having a dielectric constant in the range of no greater than 115; and a pH buffer system, wherein the target nucleic acid is attached to the surface via hybridization to a surface bound nucleic acid tethered to the surface, and wherein the hybridization of the target nucleic acid and surface bound nucleic acid has a high stringency and annealing rate.

公开(公告)号：US20220403463A1

公开(公告)日：2022-12-22

申请号：US17377279

申请日：2021-07-15

Applicant: Element Biosciences, Inc.

Inventor： Sinan ARSLAN ,  Junhua ZHAO ,  Molly HE ,  Samantha SNOW ,  William LIGHT ,  Matthew KELLINGER ,  Michael PREVITE ,  Michael KIM ,  Hua YU ,  Yu-Hsien HWANG-FU ,  Marco TJIOE ,  Andrew BODDICKER

IPC: C12Q1/6874

Abstract: The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g., having nucleotide units), the signals emitted by the nucleotides or nucleotide units that participate in the parallel sequencing reactions along the concatemer yields an increased signal intensity for each concatemer.

公开(公告)号：US20220267842A1

公开(公告)日：2022-08-25

申请号：US17743729

申请日：2022-05-13

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling CHEN ,  Minghao GUO ,  Michael PREVITE ,  Chunhong ZHOU ,  Derek FULLER

IPC: C12Q1/6869 ,  G01N21/64 ,  G01N15/14 ,  C12N15/10 ,  C12Q1/6806

Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.

公开(公告)号：US20220251643A1

公开(公告)日：2022-08-11

申请号：US17723275

申请日：2022-04-18

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling CHEN ,  Minghao GUO ,  Michael PREVITE ,  Chunhong ZHOU ,  Derek FULLER

IPC: C12Q1/6869 ,  G01N21/64 ,  G01N15/14 ,  C12N15/10 ,  C12Q1/6806

Abstract: Methods and systems for sequencing a nucleic acid molecule are described that comprise imaging a first surface and an axially-displaced second surface using a compensation-free optical system, the system comprising an objective lens and at least one image sensor, wherein said optical system has a numerical aperture (NA) of less than 0.6 and a field-of-view (FOV) of greater than 1.0 mm2; and) processing the images of the first surface and the axially-displaced second surface to correct for optical aberration such that the images of the first surface and the axially-displaced second surface have substantially the same optical resolution.

公开(公告)号：US20210332416A1

公开(公告)日：2021-10-28

申请号：US17372361

申请日：2021-07-09

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling CHEN ,  Minghao GUO ,  Michael PREVITE ,  Chunhong ZHOU ,  Derek FULLER

IPC: C12Q1/6806 ,  C12N15/10 ,  G01N21/64

Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.