公开(公告)号：US5256575A

公开(公告)日：1993-10-26

申请号：US755203

申请日：1991-09-04

申请人： Mae W. Hu ,  Kirk Schulkamp ,  Cheng-I Lin ,  Edwin F. Ullman

发明人： Mae W. Hu ,  Kirk Schulkamp ,  Cheng-I Lin ,  Edwin F. Ullman

IPC分类号： C07C235/34 ,  C07C235/52 ,  C07C259/10 ,  C07C309/15 ,  G01N33/53 ,  G01N33/78 ,  G01N33/94 ,  G01N33/566

CPC分类号： C07C309/15 ,  C07C235/34 ,  C07C235/52 ,  C07C259/10 ,  G01N33/5306 ,  G01N33/78 ,  G01N33/94 ,  Y10S435/962 ,  Y10S435/975 ,  Y10S436/825

摘要： A kit for inactivating interfering binding proteins in a immunoassay for a member of a specific binding pair (sbp). The method comprises including in an assay medium containing a sample suspected of containing an sbp member and an interfering binding protein an effective amount of a water soluble compound having two substituted or unsubstituted phenyl groups linked to a common atom. When the sbp member or its sbp partner has two phenyl groups linked to a common atom, the compound has a number of-groups other than hydrogen attached to the phenyl groups and the atom that differs by at least two from the number of such groups on the sbp member. When the sbp member or its sbp partner has two phenyl groups linked to a common atom and the binding protein is not an antibody, the compound has only one group other than hydrogen attached to a phenyl group or the common atom. The methods have particular application in avoiding-cross-reactivity of non-analyte materials in a sample with immunachemical reagents used in such assay. The methods-have application also in disrupting complexer between an analyte to be determined and other materials to that one can accurately determine the amount of an analyte in a sample.

摘要翻译： 用于在特异性结合对（sbp）的成员的免疫测定中灭活干扰结合蛋白的试剂盒。 该方法包括在含有疑似含有sbp成员和干扰结合蛋白的样品的测定培养基中包含有效量的具有两个与常见原子连接的取代或未取代的苯基的水溶性化合物。 当sbp成员或其sbp伴侣具有连接到共同原子的两个苯基时，该化合物具有与苯基连接的氢以外的多个基团，并且与该基团的数目不同的至少两个的原子在 sbp成员。 当sbp成员或其sbp伴侣具有连接到共同原子的两个苯基并且结合蛋白不是抗体时，该化合物除了连接到苯基或共同原子上的氢以外仅具有一个基团。 该方法特别适用于避免样品中非分析物质与这种测定中使用的免疫化学试剂的交叉反应。 所述方法还应用于破坏要测定的待分析物与其他材料之间的复合物，以使得可以准确地确定样品中分析物的量。

公开(公告)号：US5136095A

公开(公告)日：1992-08-04

申请号：US278870

申请日：1988-12-01

申请人： Thomas L. Tarnowski ,  Cheng-I Lin ,  Edwin F. Ullman

发明人： Thomas L. Tarnowski ,  Cheng-I Lin ,  Edwin F. Ullman

IPC分类号： G01N33/543

CPC分类号： G01N33/54333 ,  G01N33/54313 ,  G01N33/54393 ,  Y10S436/808 ,  Y10S436/829

摘要： Compounds and methods are disclosed for reversibly aggregating particles suspended in a liquid medium. The method comprises combining the liquid medium containing the particles with a polyionic polymer capable of aggregating the particles under conditions suitable for such aggregation. Thereafter, the particles are contacted with a chemical reagent capable of cleaving the polyionic polymer under conditions sufficient to reverse the aggregation. Optionally, magnetic particles are added to the liquid medium in the present method under conditions for non-specific binding and the medium including the aggregates is subjected to a magnetic field gradient to separate the aggregates from the medium. The compounds of the present invention are polyions. The aggregation of the particles is reversible upon contact with chemical agents which cleave at least some of the bonds within the polyionic polymer.

摘要翻译： 公开了用于可逆地聚集悬浮在液体介质中的颗粒的化合物和方法。 该方法包括将含有颗粒的液体介质与能够在适于这种聚集的条件下聚集颗粒的聚离子聚合物组合。 此后，使颗粒与能够在足以反向聚集的条件下裂解聚离子聚合物的化学试剂接触。 任选地，在本方法中，在非特异性结合的条件下，将磁性颗粒加入到液体介质中，并且将包含聚集体的介质进行磁场梯度以将聚集体与介质分离。 本发明的化合物是聚阴离子。 当与化学试剂接触时，颗粒的聚集是可逆的，其切割聚离子聚合物内的至少一些键。

公开(公告)号：US5076950A

公开(公告)日：1991-12-31

申请号：US533622

申请日：1990-06-05

申请人： Edwin F. Ullman ,  Vartan E. Ghazarossian ,  Nurith Kurn ,  Litai Weng

发明人： Edwin F. Ullman ,  Vartan E. Ghazarossian ,  Nurith Kurn ,  Litai Weng

IPC分类号： B03C1/01 ,  C12Q1/24 ,  G01N33/538 ,  G01N33/543

CPC分类号： C12Q1/24 ,  B03C1/01 ,  G01N33/538 ,  G01N33/54333 ,  Y10S436/829

摘要： A method is disclosed for separating a substance from a liquid medium. The method comprises combining the liquid medium containing the substance with magnetic particles under conditions for non-specific chemical binding of the magnetic particles. Thereafter, the medium is subjected to a magnetic field gradient to separate the particles from the medium. The preferred non-specific binding is achieved as the result of charge interactions between the particles usually by means of a polyionic reagent. The method of the invention has particular application to the separation of cells and microorganisms from aqueous suspensions and also to the determination of an analyte in a sample suspected of containing the analyte. The analyte is a member of a specific binding pair (sbp). The sample is combined in an assay medium with magnetic particles and a sbp member complementary to the analyte. Magnetic or non-magnetic particles capable of specific binding to the analyte or its complementary sbp member must be included in the assay medium. The combination is made under conditions for non-specifically aggregating the magnetic particles or coaggregating the magnetic and non-magnetic particles when non-magnetic particles are present. The assay medium is subjected to a magnetic field gradient to separate the aggregated particles from the medium. Then, the medium or the particles are examined for the presence or amount of the analyte or an sbp member, the binding of which is affected by the presence of the analyte.

摘要翻译： 公开了一种用于从液体介质中分离物质的方法。 该方法包括在含有磁性颗粒的非特异性化学结合的条件下将含有物质的液体介质与磁性颗粒组合。 此后，对介质进行磁场梯度以将颗粒与介质分离。 优选的非特异性结合是通过颗粒之间的电荷相互作用的结果实现的，通常通过聚离子试剂。 本发明的方法特别适用于从水性悬浮液中分离细胞和微生物，以及用于测定怀疑含有分析物的样品中的分析物。 分析物是特异性结合对（sbp）的成员。 将样品在测定介质中与磁性颗粒和与分析物互补的sbp构件组合。 能够与分析物或其互补sbp成员特异性结合的磁性或非磁性粒子必须包含在测定介质中。 在非磁性颗粒存在的情况下，在非特异性聚集磁性颗粒或使磁性和非磁性颗粒共聚的条件下进行组合。 对测定介质进行磁场梯度以将聚集的颗粒与介质分离。 然后，检查介质或颗粒的分析物或sbp构件的存在或量，其结合受分析物的存在的影响。

公开(公告)号：US5068198A

公开(公告)日：1991-11-26

申请号：US298192

申请日：1989-01-13

申请人： Ian Gibbons ,  Edwin F. Ullman ,  Philip L. Felgner

发明人： Ian Gibbons ,  Edwin F. Ullman ,  Philip L. Felgner

IPC分类号： G01N33/543 ,  G01N33/549

CPC分类号： G01N33/549 ,  G01N33/54313 ,  Y10S436/829

摘要： Assay methods and compositions are provided for determining an analyte in a sample suspected of containing the analyte. The composition comprises in a novel single liquid reagent at least one specific binding pair (sbp) member and its complementary member wherein at least one sbp member is reversibly confined in a material that temporarily renders the confined sbp member incapable of binding with its complementary sbp member. At least one of the sbp members is bound to a member of a signal producing system capable of producing a detectable signal in relation to the amount of analyte in the sample. The confinement is reversed, any remaining members of the signal producing system are added, and the signal produced in relation to the amount of analyte is measured. Examples of the confining material are lipid bilayers, cells and gels.

摘要翻译： 提供测定方法和组合物用于测定怀疑含有分析物的样品中的分析物。 组合物包含新颖的单液体试剂至少一种特异性结合对（sbp）构件及其互补构件，其中至少一个sbp构件可逆地限制在暂时使限制的sbp构件不能与其互补的sbp构件结合的材料中 。 sbp成员中的至少一个与能够产生与样品中分析物的量相关的可检测信号的信号产生系统的成员结合。 约束相反，添加信号产生系统的任何剩余成员，并且测量相对于分析物量产生的信号。 限制材料的实例是脂双层，细胞和凝胶。

公开(公告)号：US4935147A

公开(公告)日：1990-06-19

申请号：US262771

申请日：1988-10-26

申请人： Edwin F. Ullman ,  Vartan E. Ghazarossian ,  Nurith Kurn ,  Litai Weng

发明人： Edwin F. Ullman ,  Vartan E. Ghazarossian ,  Nurith Kurn ,  Litai Weng

IPC分类号： B03C1/01 ,  C12Q1/24 ,  G01N33/538 ,  G01N33/543

CPC分类号： B03C1/01 ,  C12Q1/24 ,  G01N33/538 ,  G01N33/54333

摘要： A method is disclosed for separating a substance from a liquid medium. The method comprises combining the liquid medium containing the substance with magnetic particles under conditions for non-specific chemical binding of the magnetic particles. Thereafter, the medium is subjected to a magnetic field gradient to separate the particles from the medium. The preferred non-specific binding is achieved as the result of charge interactions between the particles usually by means of a polyionic reagent. The method of the invention has particular application to the separation of cells and microorganisms from aqueous suspensions and also to the determination of an analyte in a sample suspected of containing the analyte. The analyte is a member of a specific binding pair (sbp). The sample is combined in an assay medium with magnetic particles and a sbp member complementary to the analyte. Magnetic or non-magnetic particles capable of specific binding to the analyte or its complementary sbp member must be included in the assay medium. The combination is made under conditions for non-specifically aggregating the magnetic particles or coaggregating the magnetic and non-magnetic particles when non-magnetic particles are present. The assay medium is subjected to a magnetic field gradient to separate the aggregated particles from the medium. Then, the medium or the particles are examined for the presence or amount of the analyte or an sbp member, the binding of which is affected by the presence of the analyte.

摘要翻译： 公开了一种用于从液体介质中分离物质的方法。 该方法包括在含有磁性颗粒的非特异性化学结合的条件下将含有物质的液体介质与磁性颗粒组合。 此后，对介质进行磁场梯度以将颗粒与介质分离。 优选的非特异性结合是通过颗粒之间的电荷相互作用的结果实现的，通常通过聚离子试剂。 本发明的方法特别适用于从水性悬浮液中分离细胞和微生物，以及用于测定怀疑含有分析物的样品中的分析物。 分析物是特异性结合对（sbp）的成员。 将样品在测定介质中与磁性颗粒和与分析物互补的sbp构件组合。 能够与分析物或其互补sbp成员特异性结合的磁性或非磁性粒子必须包含在测定介质中。 在非磁性颗粒存在的情况下，在非特异性聚集磁性颗粒或使磁性和非磁性颗粒共聚的条件下进行组合。 对测定介质进行磁场梯度以将聚集的颗粒与介质分离。 然后，检查介质或颗粒的分析物或sbp构件的存在或量，其结合受分析物的存在的影响。

公开(公告)号：US4748129A

公开(公告)日：1988-05-31

申请号：US645458

申请日：1984-08-28

申请人： Chiu C. Chang ,  Vartan Ghazarossian ,  Edwin F. Ullman

发明人： Chiu C. Chang ,  Vartan Ghazarossian ,  Edwin F. Ullman

IPC分类号： G01N33/52 ,  A61K39/00 ,  G01N33/53 ,  G01N33/533 ,  G01N33/536 ,  G01N33/542 ,  G01N33/554 ,  G01N33/555 ,  G01N33/58 ,  G01N33/80

CPC分类号： G01N33/555 ,  G01N33/533 ,  G01N33/542 ,  G01N33/80 ,  Y10S436/80 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/829

摘要： A method is provided for determining the presence in a sample of a member of a specific binding pair ("sbp member") consisting of ligand and its homologous receptor. The sample is combined in an aqueous medium with (1) a complementary sbp member wherein at least the sbp member or the complementary sbp member is bound to the surface of a cell and (2) a fuorescent agent capable of being incorporated into the cell. The presence of the sbp member is indicated by a change in fluorescence of the unseparated cell suspension as a result of agglutination of the cells.The present invention has particular application to blood typing, for example, for the determination of the presence of blood group antigens A, B, AB, O, and D (Rh.sub.o) and antibodies to such antigens.

摘要翻译： 提供了一种用于确定由配体及其同源受体组成的特异性结合对（“sbp成员”）的成员的样品中存在的方法。 样品在水性介质中与（1）互补sbp构件组合，其中至少sbp构件或互补sbp构件与细胞表面结合，和（2）能够结合到细胞中的荧光剂。 由于细胞凝集，未分离的细胞悬浮液的荧光变化表示sbp成员的存在。 本发明特别适用于血型分型，例如用于测定血型抗原A，B，AB，O和D（Rho）及其抗体的存在。

公开(公告)号：US4727022A

公开(公告)日：1988-02-23

申请号：US589428

申请日：1984-03-14

申请人： Carl Skold ,  Dennis R. Gould ,  Edwin F. Ullman

发明人： Carl Skold ,  Dennis R. Gould ,  Edwin F. Ullman

IPC分类号： G01N33/542 ,  G01N33/68 ,  G01N33/535 ,  C12N9/96 ,  C12N9/99 ,  G01N33/563

CPC分类号： G01N33/6878 ,  G01N33/542 ,  Y10S435/81 ,  Y10S435/964 ,  Y10S436/808

摘要： Methods are provided for modulating Ligand-Receptor interactions by binding of molecules at two epitopes of a receptor, where the epitopes are in relatively close special relationship. By providing for inhibition of changes in conformation of the receptor, where the inhibition is due to steric interactions or molecular bridging between the two epitopic sites, Ligand-Receptor interactions may be modulated. The modulation of Ligand-Receptor interactions has application to diagnostic assays, modulation of cellular activity, and modulation of the physiological activity of macromolecular compounds.

摘要翻译： 提供了通过结合受体的两个表位处的分子来调节配体受体相互作用的方法，其中表位处于相对紧密的特殊关系。 通过提供对受体构象变化的抑制，其中抑制是由于两个表位位点之间的空间相互作用或分子桥接引起的，可以调节配体 - 受体相互作用。 配体 - 受体相互作用的调控可用于诊断测定，调节细胞活性和调节大分子化合物的生理活性。

公开(公告)号：US4650770A

公开(公告)日：1987-03-17

申请号：US559555

申请日：1983-12-07

申请人： Yen-Ping Liu ,  Edwin F. Ullman ,  Martin J. Becker

发明人： Yen-Ping Liu ,  Edwin F. Ullman ,  Martin J. Becker

IPC分类号： G01N33/542 ,  G01N33/543 ,  G01N33/533 ,  G01N33/546

CPC分类号： G01N33/542 ,  Y10S436/805

摘要： Assays are provided employing particles and absorbent particles, wherein the absorbent particles substantially inhibit fluorescence when bound to the fluorescent particles through specific non-covalent binding.

摘要翻译： 使用颗粒和吸收剂颗粒提供测定，其中当通过特定的非共价结合与吸附剂颗粒结合时，吸收颗粒基本上抑制荧光。

公开(公告)号：US4624929A

公开(公告)日：1986-11-25

申请号：US677144

申请日：1984-12-03

申请人： Edwin F. Ullman

发明人： Edwin F. Ullman

IPC分类号： B01L3/00 ,  B01L3/14 ,  G01N33/53 ,  G01N33/558 ,  G01N37/00 ,  G01N1/10

CPC分类号： B01L3/50825 ,  G01N33/5304 ,  G01N33/558 ,  B01L2300/047 ,  B01L2300/069 ,  B01L2400/0406 ,  B01L3/5023 ,  Y10S435/962 ,  Y10S435/966 ,  Y10T436/25625

摘要： A device is disclosed for collecting a liquid sample and diluting the liquid sample by virtue of being adapted for use with a container having a volume of diluting liquid. The device comprises a housing adapted for mating with the container where the mated housing and container form a chamber. A bibulous pad is attached to the housing for collecting a predetermined amount of a liquid sample. Additionally, the device, when used with the container, creates a pressure differential between the interior of the housing and the chamber. The pressure differential is sufficient to move a predetermined volume of the diluting liquid through the bibulous pad and into the housing. The device can be used for assaying for a component of a sample. The device has particular use in immunochromatography and for convenience can also contain a strip of bibulous material confined in the housing. In a method for collecting a liquid sample and subsequently diluting the sample with a predetermined volume of a liquid, the sample is collected on an bibulous pad at the base of a housing. The pad is designed to absorb a predetermined amount of the sample. Next, a pressure differential is created between the housing and a container having a volume of diluting liquid to cause a predetermined volume of the liquid to pass through the pad and into the housing.

摘要翻译： 公开了一种用于收集液体样品并通过适于与具有一定体积的稀释液体的容器一起使用来稀释液体样品的装置。 该装置包括适于与配合的壳体和容器形成室的容器配合的壳体。 将吸水垫连接到壳体上以收集预定量的液体样品。 此外，当与容器一起使用时，该装置在壳体的内部和腔室之间产生压力差。 压力差足以使预定体积的稀释液体通过吸水垫移动到壳体中。 该装置可用于测定样品组分。 该装置在免疫层析中特别有用，为了方便起见，还可以包含限制在壳体中的吸水带材料。 在用于收集液体样品并随后用预定体积的液体稀释样品的方法中，将样品收集在壳体底部的吸水垫上。 该垫被设计成吸收预定量的样品。 接下来，在壳体和具有一定体积的稀释液体的容器之间产生压力差，以使预定体积的液体通过垫并进入壳体。

公开(公告)号：US4584277A

公开(公告)日：1986-04-22

申请号：US482124

申请日：1983-04-05

申请人： Edwin F. Ullman

发明人： Edwin F. Ullman

IPC分类号： G01N33/52 ,  G01N21/76 ,  G01N33/53 ,  G01N33/543 ,  G01N33/58 ,  G01N33/80 ,  G01N33/566 ,  G01N33/544 ,  G01N33/545

CPC分类号： G01N33/582 ,  G01N33/80 ,  Y10S436/80 ,  Y10S436/805 ,  Y10S436/808

摘要： Methods are provided for rapidly determining a number of parameters in a few determinations. Particularly, the method is applicable to blood typing, determining the blood type as to the ABO and Rh type, as well as the determination of isoantibodies to the antigens. The method employs fluorescent particles having a plurality of fluorescers, where the presence or absence of light emission of a particular wavelength can be determined.