公开(公告)号：US5256575A

公开(公告)日：1993-10-26

申请号：US755203

申请日：1991-09-04

申请人： Mae W. Hu ,  Kirk Schulkamp ,  Cheng-I Lin ,  Edwin F. Ullman

发明人： Mae W. Hu ,  Kirk Schulkamp ,  Cheng-I Lin ,  Edwin F. Ullman

IPC分类号： C07C235/34 ,  C07C235/52 ,  C07C259/10 ,  C07C309/15 ,  G01N33/53 ,  G01N33/78 ,  G01N33/94 ,  G01N33/566

CPC分类号： C07C309/15 ,  C07C235/34 ,  C07C235/52 ,  C07C259/10 ,  G01N33/5306 ,  G01N33/78 ,  G01N33/94 ,  Y10S435/962 ,  Y10S435/975 ,  Y10S436/825

摘要： A kit for inactivating interfering binding proteins in a immunoassay for a member of a specific binding pair (sbp). The method comprises including in an assay medium containing a sample suspected of containing an sbp member and an interfering binding protein an effective amount of a water soluble compound having two substituted or unsubstituted phenyl groups linked to a common atom. When the sbp member or its sbp partner has two phenyl groups linked to a common atom, the compound has a number of-groups other than hydrogen attached to the phenyl groups and the atom that differs by at least two from the number of such groups on the sbp member. When the sbp member or its sbp partner has two phenyl groups linked to a common atom and the binding protein is not an antibody, the compound has only one group other than hydrogen attached to a phenyl group or the common atom. The methods have particular application in avoiding-cross-reactivity of non-analyte materials in a sample with immunachemical reagents used in such assay. The methods-have application also in disrupting complexer between an analyte to be determined and other materials to that one can accurately determine the amount of an analyte in a sample.

摘要翻译： 用于在特异性结合对（sbp）的成员的免疫测定中灭活干扰结合蛋白的试剂盒。 该方法包括在含有疑似含有sbp成员和干扰结合蛋白的样品的测定培养基中包含有效量的具有两个与常见原子连接的取代或未取代的苯基的水溶性化合物。 当sbp成员或其sbp伴侣具有连接到共同原子的两个苯基时，该化合物具有与苯基连接的氢以外的多个基团，并且与该基团的数目不同的至少两个的原子在 sbp成员。 当sbp成员或其sbp伴侣具有连接到共同原子的两个苯基并且结合蛋白不是抗体时，该化合物除了连接到苯基或共同原子上的氢以外仅具有一个基团。 该方法特别适用于避免样品中非分析物质与这种测定中使用的免疫化学试剂的交叉反应。 所述方法还应用于破坏要测定的待分析物与其他材料之间的复合物，以使得可以准确地确定样品中分析物的量。

公开(公告)号：US5063165A

公开(公告)日：1991-11-05

申请号：US289998

申请日：1988-12-22

申请人： Mae W. Hu ,  Kirk Schulkamp ,  Cheng-I Lin ,  Edwin F. Ullman

发明人： Mae W. Hu ,  Kirk Schulkamp ,  Cheng-I Lin ,  Edwin F. Ullman

IPC分类号： C07C235/34 ,  C07C235/52 ,  C07C259/10 ,  C07C309/15 ,  G01N33/53 ,  G01N33/78 ,  G01N33/94

CPC分类号： G01N33/94 ,  C07C235/34 ,  C07C235/52 ,  C07C259/10 ,  C07C309/15 ,  G01N33/5306 ,  G01N33/78 ,  Y10S436/815 ,  Y10S436/825 ,  Y10S436/826

摘要： Methods are disclosed for inactivating interfering binding proteins in a immunoassay for a member of a specific binding pair (sbp). The method comprises including in an assay medium containing a sample suspected of containing an sbp member and an interfering binding protein an effective amount of a water soluble compound having two substituted or unsubstituted phenyl groups linked to a common atom. When the sbp member or its sbp partner has two phenyl groups linked to a common atom, the compound has a number of groups other than hydrogen attached to the phenyl groups and the atom that differs by at least two from the number of such groups on the sbp member. When the sbp member or its sbp partner has two phenyl groups linked to a common atom and the binding protein is not an antibody, the compound has only one group other than hydrogen attached to a phenyl group or the common atom. The methods have particular application in avoiding cross-reactivity of non-analyte materials in a sample with immunochemical reagents used in such assay. The methods have application also in disrupting complexes between an analyte to be determined and other materials so that one can accurately determine the amount of an analyte in a sample.

公开(公告)号：US5378636A

公开(公告)日：1995-01-03

申请号：US105834

申请日：1993-08-12

申请人： Mae W.-L. Hu ,  Kirk Schulkamp ,  Cheng-I Lin ,  Edwin F. Ullman

发明人： Mae W.-L. Hu ,  Kirk Schulkamp ,  Cheng-I Lin ,  Edwin F. Ullman

IPC分类号： C07C235/34 ,  C07C235/52 ,  C07C259/10 ,  C07C309/15 ,  G01N33/53 ,  G01N33/78 ,  G01N33/94 ,  G01N33/531

CPC分类号： C07C309/15 ,  C07C235/34 ,  C07C235/52 ,  C07C259/10 ,  G01N33/5306 ,  G01N33/78 ,  G01N33/94 ,  Y10S435/962 ,  Y10S435/975 ,  Y10S436/825

摘要： Methods are disclosed for inactivating interfering binding proteins in a immunoassay for a member of a specific binding pair (sbp). The method comprises including in an assay medium containing a sample suspected of containing an sbp member and an interfering binding protein an effective amount of a water soluble compound having two substituted or unsubstituted phenyl groups linked to a common atom. When the sbp member or its sbp partner has two phenyl groups linked to a common atom, the compound has a number of groups other than hydrogen attached to the phenyl groups and the atom that differs by at least two from the number of such groups on the sbp member. When the sbp member or its sbp partner has two phenyl groups linked to a common atom and the binding protein is not an antibody, the compound has only one group other than hydrogen attached to a phenyl group or the common atom. The methods have particular application in avoiding cross-reactivity of non-analyte materials in a sample with immunochemical reagents used in such assay. The methods have application also in disrupting complexes between an analyte to be determined and other materials so that one can accurately determine the amount of an analyte in a sample.

摘要翻译： 公开了用于在特异性结合对（sbp）的成员的免疫测定中灭活干扰结合蛋白的方法。 该方法包括在含有疑似含有sbp成员和干扰结合蛋白的样品的测定培养基中包含有效量的具有两个与常见原子连接的取代或未取代的苯基的水溶性化合物。 当sbp成员或其sbp伴侣具有连接到共同原子的两个苯基时，该化合物具有除苯基之外的氢以外的多个基团，并且与该基团上的这些基团的数目不同的至少两个的原子 sbp成员。 当sbp成员或其sbp伴侣具有连接到共同原子的两个苯基并且结合蛋白不是抗体时，该化合物除了连接到苯基或共同原子上的氢以外仅具有一个基团。 该方法特别适用于避免样品中非分析物质与这种测定中使用的免疫化学试剂的交叉反应。 该方法还可用于破坏要测定的分析物与其它材料之间的复合物，从而可以准确地确定样品中分析物的量。

公开(公告)号：US5328828A

公开(公告)日：1994-07-12

申请号：US916568

申请日：1992-07-20

申请人： Mae W. Hu ,  Cheng-I Lin ,  Chen-jung Hsu ,  James V. Freeman ,  Marcel Pirio ,  Edwin F. Ullman

发明人： Mae W. Hu ,  Cheng-I Lin ,  Chen-jung Hsu ,  James V. Freeman ,  Marcel Pirio ,  Edwin F. Ullman

IPC分类号： G01N33/53 ,  C07C233/18 ,  C07C323/12 ,  C07C323/29 ,  C07C323/32 ,  C07C323/41 ,  C07C323/60 ,  G01N33/535 ,  G01N33/577 ,  G01N33/94 ,  G01N33/536 ,  G01N33/537 ,  G01N33/542

CPC分类号： G01N33/946 ,  C07C233/18 ,  C07C323/12 ,  C07C323/32 ,  C07C323/41 ,  G01N33/535 ,  Y10S435/964 ,  Y10S436/816 ,  Y10S436/822

摘要： The instant invention is directed toward an immunoassay which can determine the presence of amphetamines in a sample suspected of containing amphetamine and/or methamphetamine by employing at least two conjugates, each comprised of a functionally similar label bound to an amphetamine analog and a methamphetamine analog respectively and an antibody to amphetamine and an antibody to methamphetamine wherein at least one of the antibodies is a monoclonal antibody.

摘要翻译： 本发明涉及免疫测定，其可以通过使用至少两种共轭物来确定怀疑含有苯丙胺和/或甲基苯丙胺的样品中苯丙胺的存在，每个缀合物分别与分别与苯丙胺类似物和甲基苯丙胺类似物结合的功能相似的标记 以及针对苯丙胺的抗体和针对甲基苯丙胺的抗体，其中至少一种抗体是单克隆抗体。

公开(公告)号：US5136095A

公开(公告)日：1992-08-04

申请号：US278870

申请日：1988-12-01

申请人： Thomas L. Tarnowski ,  Cheng-I Lin ,  Edwin F. Ullman

发明人： Thomas L. Tarnowski ,  Cheng-I Lin ,  Edwin F. Ullman

IPC分类号： G01N33/543

CPC分类号： G01N33/54333 ,  G01N33/54313 ,  G01N33/54393 ,  Y10S436/808 ,  Y10S436/829

摘要： Compounds and methods are disclosed for reversibly aggregating particles suspended in a liquid medium. The method comprises combining the liquid medium containing the particles with a polyionic polymer capable of aggregating the particles under conditions suitable for such aggregation. Thereafter, the particles are contacted with a chemical reagent capable of cleaving the polyionic polymer under conditions sufficient to reverse the aggregation. Optionally, magnetic particles are added to the liquid medium in the present method under conditions for non-specific binding and the medium including the aggregates is subjected to a magnetic field gradient to separate the aggregates from the medium. The compounds of the present invention are polyions. The aggregation of the particles is reversible upon contact with chemical agents which cleave at least some of the bonds within the polyionic polymer.

摘要翻译： 公开了用于可逆地聚集悬浮在液体介质中的颗粒的化合物和方法。 该方法包括将含有颗粒的液体介质与能够在适于这种聚集的条件下聚集颗粒的聚离子聚合物组合。 此后，使颗粒与能够在足以反向聚集的条件下裂解聚离子聚合物的化学试剂接触。 任选地，在本方法中，在非特异性结合的条件下，将磁性颗粒加入到液体介质中，并且将包含聚集体的介质进行磁场梯度以将聚集体与介质分离。 本发明的化合物是聚阴离子。 当与化学试剂接触时，颗粒的聚集是可逆的，其切割聚离子聚合物内的至少一些键。

公开(公告)号：US5370993A

公开(公告)日：1994-12-06

申请号：US881987

申请日：1992-05-12

申请人： Thomas L. Tarnowski ,  Cheng-I Lin ,  Edwin F. Ullman

发明人： Thomas L. Tarnowski ,  Cheng-I Lin ,  Edwin F. Ullman

IPC分类号： G01N33/543 ,  G01N33/53

CPC分类号： G01N33/54333 ,  G01N33/54313 ,  G01N33/54393 ,  Y10S436/808 ,  Y10S436/829

摘要： Compounds and methods are disclosed for reversibly aggregating particles suspended in a liquid medium. The method comprises combining the liquid medium containing the particles with a polyionic polymer capable of aggregating the particles under conditions suitable for such aggregation. Thereafter, the particles are contacted with a chemical reagent capable of cleaving the polyionic polymer under conditions sufficient to reverse the aggregation. Optionally, magnetic particles are added to the liquid medium in the present method under conditions for non-specific binding and the medium including the aggregates is subjected to a magnetic field gradient to separate the aggregates from the medium. The compounds of the present invention are polyions. The aggregation of the particles is reversible upon contact with chemical agents which cleave at least some of the bonds within the polyionic polymer.

摘要翻译： 公开了用于可逆地聚集悬浮在液体介质中的颗粒的化合物和方法。 该方法包括将含有颗粒的液体介质与能够在适于这种聚集的条件下聚集颗粒的聚离子聚合物组合。 此后，使颗粒与能够在足以反向聚集的条件下裂解聚离子聚合物的化学试剂接触。 任选地，在本方法中，在非特异性结合的条件下，将磁性颗粒加入到液体介质中，并且将包含聚集体的介质进行磁场梯度以将聚集体与介质分离。 本发明的化合物是聚阴离子。 当与化学试剂接触时，颗粒的聚集是可逆的，其切割聚离子聚合物内的至少一些键。

公开(公告)号：US5135863A

公开(公告)日：1992-08-04

申请号：US290487

申请日：1988-12-23

申请人： Mae Wan Leng Hu ,  Cheng-I Lin ,  Chen-jung Hsu ,  James V. Freeman ,  Marcel Pirio ,  Edwin F. Ullman

发明人： Mae Wan Leng Hu ,  Cheng-I Lin ,  Chen-jung Hsu ,  James V. Freeman ,  Marcel Pirio ,  Edwin F. Ullman

IPC分类号： G01N33/53 ,  C07C233/18 ,  C07C323/12 ,  C07C323/29 ,  C07C323/32 ,  C07C323/41 ,  C07C323/60 ,  G01N33/535 ,  G01N33/577 ,  G01N33/94

CPC分类号： G01N33/946 ,  C07C233/18 ,  C07C323/12 ,  C07C323/32 ,  C07C323/41 ,  G01N33/535 ,  Y10S435/964 ,  Y10S436/816 ,  Y10S436/822

摘要： The instant invention is directed toward an immunoassay which can determine the presence of amphetamines in a sample suspected of containing amphetamine and/or methamphetamine by employing at least two conjugates, each comprised of a functionally similar label bound to an amphetamine analog and a methamphetamine analog respectively and an antibody to amphetamine and an antibody to methamphetamine wherein at least one of the antibodies is a monoclonal antibody.

公开(公告)号：US5089390A

公开(公告)日：1992-02-18

申请号：US93454

申请日：1987-09-04

申请人： Dariush Davalian ,  Cheng-I Lin ,  Edwin F. Ullman

发明人： Dariush Davalian ,  Cheng-I Lin ,  Edwin F. Ullman

IPC分类号： G01N33/533 ,  G01N33/535 ,  G01N33/94

CPC分类号： G01N33/533 ,  G01N33/535 ,  G01N33/9493 ,  Y10S435/964 ,  Y10S435/975 ,  Y10S436/808 ,  Y10S436/815 ,  Y10S436/822

摘要： 2-methyl-4-hexene- and 3-methyl-5-heptene-1,2-diol derivatives are disclosed together with methods for preparing such derivatives. Where the derivative is a 2-methyl-4-hexene- or 3-methyl-5-heptene-1,2-diol conjugated to a label, the conjugates are useful in immunoassays. Where the 2-methyl-4-hexene or 3-methyl-5-heptene-1,2-diol is conjugated to an immunogenic carrier, the conjugates may be employed as an immunogen for use in the preparation of antibodies. The label conjugate and the antibodies can be utilized in an immunoassay for the determination or detection of cyclosporin in a sample suspected of containing cyclosporin.

摘要翻译： 公开了2-甲基-4-己烯 - 和3-甲基-5-庚烯-1,2-二醇衍生物及其制备方法。 当衍生物与标记物缀合的2-甲基-4-己烯或3-甲基-5-庚烯-1,2-二醇时，缀合物可用于免疫测定。 当2-甲基-4-己烯或3-甲基-5-庚烯-1,2-二醇与免疫原性载体缀合时，缀合物可用作用于制备抗体的免疫原。 标记结合物和抗体可用于免疫测定中，用于测定或检测怀疑含有环孢菌素的样品中的环孢菌素。

公开(公告)号：US5532138A

公开(公告)日：1996-07-02

申请号：US263164

申请日：1994-06-21

申请人： Sharat Singh ,  Arthur C. Switchenko ,  Cheng-I Lin ,  Nurith Kurn ,  Edwin F. Ullman

发明人： Sharat Singh ,  Arthur C. Switchenko ,  Cheng-I Lin ,  Nurith Kurn ,  Edwin F. Ullman

IPC分类号： C12Q1/28 ,  G01N33/546

CPC分类号： C12Q1/28 ,  C12Q2326/10 ,  C12Q2326/50 ,  Y10S435/975

摘要： Methods and compositions are disclosed for determining a peroxidatively active catalyst. The methods comprise the step of detecting a substance formed by the coupling reaction of (a) the product of the peroxidatively active catalyst-catalyzed oxidation of a benzidine with (b) a coupler other than benzidine. The methods have application in a wide variety of systems including assays for analytes. Also disclosed are kits for conducting methods and assays in accordance with the present invention.

摘要翻译： 公开了用于测定过氧化物活性催化剂的方法和组合物。 该方法包括检测由（a）联苯胺与（b）除联苯胺以外的成色剂的过氧化活性催化剂催化氧化产物的偶联反应形成的物质的步骤。 该方法可用于各种系统，包括分析物的测定。 还公开了用于进行根据本发明的方法和测定的试剂盒。

公开(公告)号：US5405743A

公开(公告)日：1995-04-11

申请号：US267636

申请日：1994-06-29

申请人： Thomas L. Tarnowski ,  Cheng-I Lin ,  Edwin F. Ullman

发明人： Thomas L. Tarnowski ,  Cheng-I Lin ,  Edwin F. Ullman

IPC分类号： G01N33/543 ,  C12N5/06

CPC分类号： G01N33/54333 ,  G01N33/54313 ,  G01N33/54393 ,  Y10S436/808 ,  Y10S436/829

摘要： Compounds and methods are disclosed for reversibly aggregating particles suspended in a liquid medium. The method comprises combining the liquid medium containing the particles with a polyionic polymer capable of aggregating the particles under conditions suitable for such aggregation. Thereafter, the particles are contacted with a chemical reagent capable of cleaving the polyionic polymer under conditions sufficient to reverse the aggregation. Optionally, magnetic particles are added to the liquid medium in the present method under conditions for non-specific binding and the medium including the aggregates is subjected to a magnetic field gradient to separate the aggregates from the medium. The compounds of the present invention are polyions. The aggregation of the particles is reversible upon contact with chemical agents which cleave at least some of the bonds within the polyionic polymer.