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50 条记录 (耗时 0.049 秒)

    &bgr;-secretase antibody
    47.
    发明授权
    &bgr;-secretase antibody 失效
    β-分泌酶抗体

    公开(公告)号：US06221645B1

    公开(公告)日：2001-04-24

    申请号：US08660531

    申请日：1996-06-07

    申请人： Susanna M. S. Chrysler ,  Sukanto Sinha ,  Pamela S. Keim ,  John P. Anderson ,  Hua Tan ,  Lisa Clair McConlogue

    发明人： Susanna M. S. Chrysler ,  Sukanto Sinha ,  Pamela S. Keim ,  John P. Anderson ,  Hua Tan ,  Lisa Clair McConlogue

    IPC分类号： C07K1600

    CPC分类号： C12N9/6478 ,  C07K14/4711 ,  C07K16/40 ,  C07K2319/00 ,  G01N33/573 ,  G01N33/6896

    摘要： Compositions comprising a novel protease capable of cleaving &bgr;-amyloid precursor protein (APP) on the amino-terminal side of the &bgr;-amyloid peptide therein are provided. The protease is designated &bgr;-secretase. Reaction systems comprising &bgr;-secretase may be used in screening assays to monitor &bgr;-secretase modulated cleavage of APP and to identify &bgr;-secretase inhibitors, wherein the &bgr;-secretase is in the presence of a suitable polypeptide substrate and cleavage of the substrate determined in the presence and absence of the test substance. Antibodies are raised against peptides of &bgr;-secretase. Pharmaceutical compositions and methods comprise compounds identified by screening assays.

    摘要翻译： 提供了包含能够在其中的β-淀粉样蛋白肽的氨基末端侧切割β-淀粉样蛋白前体蛋白（APP）的新型蛋白酶的组合物。 蛋白酶被指定为β-分泌酶。 包含β-分泌酶的反应体系可用于筛选测定以监测APP的β-分泌酶调节的切割并鉴定β-分泌酶抑制剂，其中β-分泌酶在合适的多肽底物存在下和在 测试物质的存在和不存在。 针对β-分泌酶肽产生抗体。 药物组合物和方法包括通过筛选测定鉴定的化合物。

    Amyloidin protease and uses thereof
    49.
    发明授权
    Amyloidin protease and uses thereof 失效
    淀粉蛋白蛋白酶及其用途

    公开(公告)号：US5424205A

    公开(公告)日：1995-06-13

    申请号：US59032

    申请日：1993-05-07

    申请人： Harry F. Dovey ,  Peter A. Seubert ,  Sukanto Sinha

    发明人： Harry F. Dovey ,  Peter A. Seubert ,  Sukanto Sinha

    IPC分类号： C07K16/40 ,  C12N9/64 ,  C12N15/57

    CPC分类号： C12N9/6424 ,  C07K16/40 ,  C12N9/6421

    摘要： A proteolytic enzyme isolated from human tissue which exhibits a proteolytic activity to hydrolyze Met-Asp peptide bond in an amyloid-like substrate is disclosed. This enzyme has been designated "amyloidin" because it proteolytically cleaves a Met-Asp bond similar to the one present in the amyloid precursor protein to release a fragment having the mature Asp terminus of the .beta.-amyloid peptide. Antibodies to the amyloidin protease are also provided. Methods to isolate and purify the amyloidin protease are provided, as well as assays to screen for inhibitors of the amyloidin protease. Also disclosed is the gene encoding the protease and methods for expression of the protease by recombinant DNA means.

    摘要翻译： 公开了从人体组织分离的蛋白水解酶，其表现出蛋白水解活性以水解淀粉样蛋白样基质中的Met-Asp肽键。 这种酶被称为“淀粉样蛋白”，因为它蛋白水解切割与淀粉样蛋白前体蛋白质中存在的Met-Asp键类似的Met-Asp键，以释放具有β-淀粉样蛋白肽成熟Asp末端的片段。 还提供了抗淀粉样蛋白酶的抗体。 提供了分离和纯化淀粉酶蛋白酶的方法，以及筛选淀粉蛋白酶蛋白酶抑制剂的测定法。 还公开了编码蛋白酶的基因和通过重组DNA手段表达蛋白酶的方法。

    Amyloidin protease and uses thereof
    50.
    发明授权
    Amyloidin protease and uses thereof 失效
    淀粉蛋白蛋白酶及其用途

    公开(公告)号：US5292652A

    公开(公告)日：1994-03-08

    申请号：US766351

    申请日：1991-09-30

    申请人： Harry F. Dovey ,  Peter A. Seubert ,  Sukanto Sinha

    发明人： Harry F. Dovey ,  Peter A. Seubert ,  Sukanto Sinha

    IPC分类号： C07K16/40 ,  C12N9/64 ,  C12N15/57

    CPC分类号： C12N9/6424 ,  C07K16/40 ,  C12N9/6421

    摘要： A proteolytic enzyme isolated from human tissue which exhibits a proteolytic activity to hydrolyze Met-Asp peptide bond in an amyloid-like substrate is disclosed. This enzyme has been designated "amyloidin" because it proteolytically cleaves a Met-Asp bond similar to the one present in the amyloid precursor protein to release a fragment having the mature Asp terminus of the .beta.-amyloid peptide. Antibodies to the amyloidin protease are also provided. Methods to isolate and purify the amyloidin protease are provided, as well as assays to screen for inhibitors of the amyloidin protease. Also disclosed is the gene encoding the protease and methods for expression of the protease by recombinant DNA means.

    摘要翻译： 公开了从人体组织分离的蛋白水解酶，其表现出蛋白水解活性以水解淀粉样蛋白样基质中的Met-Asp肽键。 这种酶被称为“淀粉样蛋白”，因为它蛋白水解地切割与淀粉样蛋白前体蛋白质中存在的Met-Asp键类似的Met-Asp键，以释放具有（β） - 淀粉样蛋白肽成熟Asp末端的片段。 还提供了抗淀粉样蛋白酶的抗体。 提供了分离和纯化淀粉酶蛋白酶的方法，以及筛选淀粉蛋白酶蛋白酶抑制剂的测定法。 还公开了编码蛋白酶的基因和通过重组DNA手段表达蛋白酶的方法。