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    Methods and compositions for targeted polynucleotide modification
    41.
    发明授权
    Methods and compositions for targeted polynucleotide modification 有权
    用于靶向多核苷酸修饰的方法和组合物

    公开(公告)号：US08704041B2

    公开(公告)日：2014-04-22

    申请号：US12982013

    申请日：2010-12-30

    申请人： William J. Gordon-Kamm ,  Keith S. Lowe ,  David J. Peterson ,  Christopher J. Scelonge ,  Grace M. St. Clair ,  Bing-Bing Wang

    发明人： William J. Gordon-Kamm ,  Keith S. Lowe ,  David J. Peterson ,  Christopher J. Scelonge ,  Grace M. St. Clair ,  Bing-Bing Wang

    IPC分类号： C12N15/82 ,  C12N15/87 ,  C12N5/04 ,  C12N5/14 ,  C07H21/02

    CPC分类号： C12N15/8216 ,  C07K14/415 ,  C12N15/8213

    摘要： A variety of methods and compostions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.

    摘要翻译： 提供了各种方法和组合物，包括用于靶向修饰细胞或生物体中特定靶位点的方法和组合物，用于整合感兴趣的多核苷酸的方法，评估启动子活性的方法，直接选择转化的生物体，最小化或消除产生的表达 从随机整合到生物体如植物的基因组中，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，使基因沉默，并鉴定和/或表征转录调节区。 该方法涉及将细胞增殖因子和双链断裂诱导酶引入生物体中。

    FRT recombination sites and methods of use
    42.
    发明授权
    FRT recombination sites and methods of use 有权
    FRT重组位点和使用方法

    公开(公告)号：US08586361B2

    公开(公告)日：2013-11-19

    申请号：US12893418

    申请日：2010-09-29

    申请人： Yumin Tao ,  Dennis L. Bidney ,  William J. Gordon-Kamm ,  Leszek A. Lyznik

    发明人： Yumin Tao ,  Dennis L. Bidney ,  William J. Gordon-Kamm ,  Leszek A. Lyznik

    IPC分类号： C12N15/87 ,  C12N15/82

    CPC分类号： C12N15/8213 ,  C12N15/102 ,  C12N15/1051 ,  C12N15/1093 ,  C40B40/08

    摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.

    摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。

    WUSCHEL(WUS) GENE HOMOLOGS
    43.
    发明申请
    WUSCHEL(WUS) GENE HOMOLOGS 有权
    WUSCHEL（WUS）基因同源物

    公开(公告)号：US20110258741A1

    公开(公告)日：2011-10-20

    申请号：US13168133

    申请日：2011-06-24

    申请人： Rebecca E. Cahoon ,  Keith S. Lowe ,  William J. Gordon-Kamm ,  Yumin Tao ,  Christopher J. Scelonge

    发明人： Rebecca E. Cahoon ,  Keith S. Lowe ,  William J. Gordon-Kamm ,  Yumin Tao ,  Christopher J. Scelonge

    IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/29

    CPC分类号： C12N15/8241 ,  C07K14/415 ,  C12N15/62 ,  C12N15/8201 ,  C12N15/8207 ,  C12N15/8261 ,  Y02A40/146

    摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.

    摘要翻译： 本发明涉及编码WUS多肽的分离的多核苷酸。 本发明还提供了分离的WUS多肽。 本发明还提供了使用多核苷酸来调节WUS水平，提高转化效率，刺激植物细胞生长（包括干细胞）刺激器官发生，刺激体细胞胚发生，诱导无融合生殖以及提供阳性选择的方法 包含多核苷酸的细胞。 本发明还涉及包含本发明的多核苷酸或通过本发明的方法产生的细胞，植物和种子。

    NOVEL FRT RECOMBINATION SITES AND METHODS OF USE
    44.
    发明申请
    NOVEL FRT RECOMBINATION SITES AND METHODS OF USE 有权
    新型FRT再现站点和使用方法

    公开(公告)号：US20110047655A1

    公开(公告)日：2011-02-24

    申请号：US12893418

    申请日：2010-09-29

    申请人： Yumin Tao ,  Dennis L. Bidney ,  William J. Gordon-Kamm ,  Leszek A. Lyznik

    发明人： Yumin Tao ,  Dennis L. Bidney ,  William J. Gordon-Kamm ,  Leszek A. Lyznik

    IPC分类号： A01H5/00 ,  C12N5/10 ,  A01H5/10 ,  C12N15/82

    CPC分类号： C12N15/8213 ,  C12N15/102 ,  C12N15/1051 ,  C12N15/1093 ,  C40B40/08

    摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.

    摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。

    Mobilization of viral genomes from T-DNA using site-specific recombination systems
    45.
    发明授权
    Mobilization of viral genomes from T-DNA using site-specific recombination systems 有权
    使用位点特异性重组系统动员来自T-DNA的病毒基因组

    公开(公告)号：US07807868B2

    公开(公告)日：2010-10-05

    申请号：US12061315

    申请日：2008-04-02

    申请人： Christopher L. Baszczynski ,  Lesek Alexander Lyznik ,  William J. Gordon-Kamm ,  Xueni Guan

    发明人： Christopher L. Baszczynski ,  Lesek Alexander Lyznik ,  William J. Gordon-Kamm ,  Xueni Guan

    IPC分类号： C12N15/82 ,  C12N15/90 ,  C12N5/10

    CPC分类号： C12N15/8213 ,  C07K2319/00 ,  C12N9/00 ,  C12N15/8203 ,  C12N15/8205 ,  C12N15/90

    摘要： The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell.

    摘要翻译： 本发明涉及用于位点特异性重组酶介导的来自T-DNA的感兴趣的病毒复制子和相关DNA的动员的方法和组合物。 本发明的方法包括农杆菌介导的T-DNA向植物细胞的转移，其中T-DNA含有病毒复制子，其侧翼是用于位点特异性重组酶的直接重复的靶位点，以及任选的与病毒连接的感兴趣的DNA 复制品。 感兴趣的DNA还可以含有重组酶的不相同的靶位点。 用于位点特异性重组酶的表达盒存在于T-DNA或植物基因组上，或瞬时引入植物细胞。 位点特异性重组酶在植物细胞中的表达导致切除病毒复制子和感兴趣的相关DNA。 然后将病毒复制子和感兴趣的DNA复制到宿主植物细胞中的高拷贝数。

    MOBILIZATION OF VIRAL GENOMES FROM T-DNA USING SITE-SPECIFIC RECOMBINATION SYSTEMS
    47.
    发明申请
    MOBILIZATION OF VIRAL GENOMES FROM T-DNA USING SITE-SPECIFIC RECOMBINATION SYSTEMS 有权
    使用现场特异性重组系统从T-DNA中动员病毒基因

    公开(公告)号：US20080282426A1

    公开(公告)日：2008-11-13

    申请号：US12061315

    申请日：2008-04-02

    申请人： Christopher L. Baszczynski ,  Lesek Alexander Lyznik ,  William J. Gordon-Kamm ,  Xueni Guan

    发明人： Christopher L. Baszczynski ,  Lesek Alexander Lyznik ,  William J. Gordon-Kamm ,  Xueni Guan

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8213 ,  C07K2319/00 ,  C12N9/00 ,  C12N15/8203 ,  C12N15/8205 ,  C12N15/90

    摘要： The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell. The compositions of the invention comprise nucleic acids, such as T-DNAs containing a viral DNA flanked by directly repeated target sites for a site-specific recombinase. The nucleic acids of the invention may additionally contain expression cassettes encoding the cognate site-specific recombinase for the target sites flanking the viral genome. The compositions of the invention further comprise Agrobacterium containing the nucleic acids of the invention. The compositions and methods of the invention have use in increasing the efficiency of agroinfection, providing high copy numbers of a DNA of interest for transient expression or for integration into a plant chromosome, and in simplifying the construction and stable maintenance of vectors for agroinfection and transformation.

    摘要翻译： 本发明涉及用于位点特异性重组酶介导的来自T-DNA的感兴趣的病毒复制子和相关DNA的动员的方法和组合物。 本发明的方法包括农杆菌介导的T-DNA向植物细胞的转移，其中T-DNA含有病毒复制子，其侧翼是用于位点特异性重组酶的直接重复的靶位点，以及任选的与病毒连接的感兴趣的DNA 复制品。 感兴趣的DNA还可以含有重组酶的不相同的靶位点。 用于位点特异性重组酶的表达盒存在于T-DNA或植物基因组上，或瞬时引入植物细胞。 位点特异性重组酶在植物细胞中的表达导致病毒复制子和感兴趣的相关DNA的切除。 然后将病毒复制子和感兴趣的DNA复制到宿主植物细胞中的高拷贝数。 本发明的组合物包含核酸，例如含有侧翼为用于位点特异性重组酶的直接重复靶位点的病毒DNA的T-DNA。 本发明的核酸可以另外含有编码针对病毒基因组侧翼的靶位点的同源位点特异性重组酶的表达盒。 本发明的组合物还包含含有本发明核酸的农杆菌。 本发明的组合物和方法用于增加农业感染的效率，为临时表达提供高拷贝数的目的DNA，或用于整合到植物染色体中，以及简化用于农业感染和转化的载体的构建和稳定维持 。