公开(公告)号：US06365571B1

公开(公告)日：2002-04-02

申请号：US09296529

申请日：1999-04-22

申请人： Tsutomu Masuda ,  Ryohei Tsuji

发明人： Tsutomu Masuda ,  Ryohei Tsuji

IPC分类号： A61K3800

CPC分类号： A61K38/12 ,  A61K38/08

摘要： There are an FGF inhibitor, a angiogenesis inhibitor and an antitumor agent which are useful as a pharmaceutical and so forth, and comprise complestatin or its derivative as an effective ingredient.

摘要翻译： 存在可用作药物等的FGF抑制剂，血管发生抑制剂和抗肿瘤剂，并且包含顺铂抑制剂或其衍生物作为有效成分。

公开(公告)号：US4968613A

公开(公告)日：1990-11-06

申请号：US224445

申请日：1988-07-26

申请人： Tsutomu Masuda ,  Hiroki Tatsumi ,  Eiichi Nakano

发明人： Tsutomu Masuda ,  Hiroki Tatsumi ,  Eiichi Nakano

IPC分类号： C12N9/02

CPC分类号： C12N9/0069 ,  C12Y113/12007

摘要： A luciferase gene isolated from Luciola cruciata (Japanese firefly) coding for an amino acid sequence shown in FIG. 4 and a novel recombinant DNA characterized by incorporating a gene coding for luciferase into a vector DNA are disclosed. There is also disclosed a method of producing luciferase which comprises culturing in a medium a microorganism containing a recombinant DNA having inserted a gene coding for luciferase in a vector DNA and belonging to the genus Escherichia capable of producing luciferase and collecting luciferase from the culture.

公开(公告)号：US4865979A

公开(公告)日：1989-09-12

申请号：US498802

申请日：1983-05-27

申请人： Eiichi Nakano ,  Tsutomu Masuda ,  Yasuji Koyama ,  Satoshi Kitao

发明人： Eiichi Nakano ,  Tsutomu Masuda ,  Yasuji Koyama ,  Satoshi Kitao

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N7/00 ,  C12N15/00 ,  C12N15/70 ,  C12N15/73 ,  C12R1/91

CPC分类号： C12N15/70 ,  C12N15/73

摘要： A bacteriophage which is characterized by the presence of one or more restriction enzyme sites downstream from the late promoter up to the cohesive end, and none or a permissible number of restriction enzyme sites upstream from the late promoter to the cohesive end is useful as an expression vector. The vector can be used to obtain high expression of DNA inserted at the restriction enzyme site downstream from the late promoter up to the cohesive end.

摘要翻译： 一种噬菌体，其特征在于存在一个或多个限制酶位点，从晚期启动子下游到粘性末端，并且没有或允许数量的限制酶位点从后期启动子上游到粘性末端可用作表达 向量。 该载体可用于获得插入在晚期启动子下游的限制酶位点直至粘性末端的DNA的高表达。

公开(公告)号：US4348477A

公开(公告)日：1982-09-07

申请号：US87166

申请日：1979-10-22

申请人： Eiichi Nakano ,  Tsutomu Masuda ,  Narimasa Saito ,  Danji Fukushima

发明人： Eiichi Nakano ,  Tsutomu Masuda ,  Narimasa Saito ,  Danji Fukushima

IPC分类号： C12N15/09 ,  C12N15/00 ,  C12P19/34 ,  C12R1/91

CPC分类号： C12N15/00

摘要： A method for preparing a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a phage DNA having an endonuclease-sensitive region not in the DNA segment participating in temperate phage DNA replication and integration of DNA into a host chromosome but in other DNA segments, a temperate phage DNA having an endonuclease-sensitive region in the DNA segment carrying genetic information for the production of coat protein, and a DNA carrying the intended genetic information, (2) mixing together all fragments produced by said cleaving, (3) adding DNA ligase to the mixture, and (4) recovering from the resulting mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segment carrying genetic information for coat protein production with a DNA fragment carrying the intended genetic information.

摘要翻译： 一种制备新型重组DNA的方法，其包括（1）用核酸内切酶切割具有不在DNA片段中的内切核酸酶敏感区域的噬菌体DNA，其参与温和噬菌体DNA复制并将DNA整合到宿主染色体中，但在其他DNA （2）将由所述切割产生的所有片段混合在一起，（3）将所述切割产生的所有片段混合在一起，其中所述DNA片段具有用于产生外壳蛋白的遗传信息的DNA段中具有内切核酸酶敏感区的温带噬菌体DNA， 向混合物中加入DNA连接酶，（4）从所得混合物中回收具有外壳蛋白产生能力的噬菌体DNA，通过用携带预期遗传信息的DNA片段替代携带用于外壳蛋白生产的遗传信息的DNA片段而缺失。