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37 条记录 (耗时 0.017 秒)

    Luminescent tool, its auxiliary member and method of preserving bioluminescent composition used in the tool and the auxiliary member
    2.
    发明授权
    Luminescent tool, its auxiliary member and method of preserving bioluminescent composition used in the tool and the auxiliary member 失效
    发光工具，其辅助构件和保存在工具中使用的生物发光组合物和辅助构件的方法

    公开(公告)号：US06521304B1

    公开(公告)日：2003-02-18

    申请号：US09331292

    申请日：1999-06-18

    申请人： Naoki Kajiyama ,  Ayumi Arai ,  Eiichi Nakano ,  Hiroki Tatsumi ,  Teruo Watarai ,  Naoki Eisaki ,  Tatsuya Sakakibara ,  Masaru Suzuki

    发明人： Naoki Kajiyama ,  Ayumi Arai ,  Eiichi Nakano ,  Hiroki Tatsumi ,  Teruo Watarai ,  Naoki Eisaki ,  Tatsuya Sakakibara ,  Masaru Suzuki

    IPC分类号： F21K206

    CPC分类号： C09K11/07 ,  F21K2/06

    摘要： The present invention relates to luminescent playthings such as candles, table lights, pen lights, illumination, illuminating playthings for camp, illuminating playthings for night fishing, fish-gathering lamps, safety candles, neon lights, luminescent inks, luminescent pens, luminescent coatings or luminescent writing implements, in which a soft light of an indescribable tone of color not obtainable from flames of conventional candles is emitted via seawater, lake and marsh water, river water, ground water, tap water or mineral drinking water by utilizing 3 components of luciferin, luciferase and ATP or 4 components of these components plus a metal salt, out of components necessary for bioluminescence in fireflies.

    摘要翻译： 本发明涉及诸如蜡烛，台灯，笔灯，照明，营地的照明玩具，夜间钓鱼的照明玩具，聚光灯，安全蜡烛，霓虹灯，发光油墨，发光笔，发光涂层或发光的发光玩具 发光书写工具，其中可以通过海水，湖泊和沼泽水，河水，地下水，自来水或矿物饮用水通过利用荧光素3的三种成分从普通蜡烛的火焰中获得难以形容的色调的柔和光线 ，荧光素酶和ATP或这些组分的4种组分加上金属盐，不仅仅是萤火虫生物发光所必需的组分。

    Purified luciferase from luciola lateralis
    5.
    发明授权
    Purified luciferase from luciola lateralis 失效
    来自luciola lateralis的纯化萤光素酶

    公开(公告)号：US5352598A

    公开(公告)日：1994-10-04

    申请号：US759814

    申请日：1991-08-29

    申请人： Naoki Kajiyama ,  Tsutomu Masuda ,  Hiroki Tatsumi ,  Eiichi Nakano

    发明人： Naoki Kajiyama ,  Tsutomu Masuda ,  Hiroki Tatsumi ,  Eiichi Nakano

    IPC分类号： C12N9/02 ,  C12N1/00

    CPC分类号： C12N9/0069 ,  C12Y113/12007 ,  Y10S435/815 ,  Y10S435/816

    摘要： A purified luciferase from Luciola lateralis is disclosed. The enzyme is characterized by having properties including: an optimum pH range of 7.5 to 9.5, an optimum temperature range of 0.degree. C. to 50.degree. C., and that the enzyme does not act on ADP, CTP, UTP, and GTP. The enzyme is purified by using a process which includes: gel filtration chromatography, hydroxyapatite column chromatography, and a tris(hydroxy)aminomethane-hydro-chloric acid buffer.

    摘要翻译： 公开了一种来自Luciola lateralis的纯化的萤光素酶。 该酶的特征在于具有以下特性：最佳pH范围为7.5至9.5，最适温度范围为0℃至50℃，并且该酶不作用于ADP，CTP，UTP和GTP。 通过使用包括凝胶过滤色谱法，羟基磷灰石柱色谱法和三（羟基）氨基甲烷 - 氢氯酸缓冲液的方法纯化酶。

    Method for desizing and scouring of a cloth
    6.
    发明授权
    Method for desizing and scouring of a cloth 失效
    一种用于织物和衣服的方法

    公开(公告)号：US5234463A

    公开(公告)日：1993-08-10

    申请号：US756486

    申请日：1991-09-09

    申请人： Yoshiteru Sando ,  Eiichi Nakano ,  Hiroshi Ishidoshiro ,  Koji Sando

    发明人： Yoshiteru Sando ,  Eiichi Nakano ,  Hiroshi Ishidoshiro ,  Koji Sando

    IPC分类号： D06B3/10 ,  D06B7/08 ,  D06L1/14 ,  D06L1/16

    CPC分类号： D06L1/16 ,  D06L1/14

    摘要： A method for desizing and scouring of a long length of cloth comprising desizing a cloth washed with hot water, and then subjecting the cloth thus washed successively to soaking with a hot alkaline sodium chlorite solution, immersing in a hot acid solution with a pH of 2-4, then to soaking with a caustic soda solution, and finally to the wet heat treatment under the ordinary pressure.

    摘要翻译： 一种长纤维布的脱浆和冲洗方法，其特征在于：将用热水洗涤的布脱浆，然后依次洗涤的衣物用热碱性亚氯酸钠溶液浸泡，浸入pH为2的热酸溶液 -4，然后用苛性钠溶液浸泡，最后在常压下进行湿热处理。

    Novel bacteriophage and method for preparing same
    9.
    发明授权
    Novel bacteriophage and method for preparing same 失效
    新型噬菌体及其制备方法

    公开(公告)号：US4332897A

    公开(公告)日：1982-06-01

    申请号：US895602

    申请日：1978-04-12

    申请人： Eiichi Nakano ,  Narimasa Saito ,  Danji Fukushima

    发明人： Eiichi Nakano ,  Narimasa Saito ,  Danji Fukushima

    IPC分类号： C12N15/09 ,  C12N7/00 ,  C12N15/00 ,  C12R1/91

    CPC分类号： C12N15/00

    摘要： A novel bacteriophage whose DNA molecule has endonuclease-sensitivity only in the DNA region carrying genetic information for the production of phage coat proteins can be obtained by isolating an endonuclease-resistant mutant from one of the lambdoid bacteriophages and mating the resulting bacteriophage with a lambdoid phage having endonuclease-sensitivity in the DNA region carrying genetic information for the production of coat proteins.

    摘要翻译： 一种新的噬菌体，其DNA分子仅在携带用于产生噬菌体外壳蛋白的遗传信息的DNA区域中具有核酸内切酶敏感性，可以通过从其中一个羊瘟噬菌体中分离内切核酸酶抗性突变体并将所得噬菌体与羊瘟噬菌体 在携带用于产生外壳蛋白的遗传信息的DNA区域中具有核酸内切酶敏感性。

    DNA fragment containing a tannase gene, a recombinant plasmid, a process for producing tannase, and a promoter
    10.
    发明授权
    DNA fragment containing a tannase gene, a recombinant plasmid, a process for producing tannase, and a promoter 失效
    含有鞣酸酶基因的DNA片段，重组质粒，鞣酸酶的制备方法和启动子

    公开(公告)号：US5665584A

    公开(公告)日：1997-09-09

    申请号：US460860

    申请日：1995-06-05

    申请人： Osamu Hatamoto ,  Teruo Watarai ,  Kiyoshi Mizusawa ,  Eiichi Nakano

    发明人： Osamu Hatamoto ,  Teruo Watarai ,  Kiyoshi Mizusawa ,  Eiichi Nakano

    IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N9/18 ,  C12N15/00 ,  C12R1/69

    CPC分类号： C12N9/18

    摘要： The present invention relates to a DNA fragment of 3,563 base pairs containing a gene coding for tannase and derived from a microorganism belonging to the genus Aspergillus, with the following restriction enzyme map: ##STR1## B: Bam HI, H: Hind III, K: Kpn I, S: Sal I, X: Xba I; a DNA fragment containing a tannase gene coding for the amino acid sequence of (SEQ ID NO:4); a recombinant plasmid comprising the DNA fragment containing the tannase gene inserted into a plasmid vector; a process for producing tannase, comprising culturing a microorganism belonging to the genus Aspergillus capable of producing tannase in medium with the recombinant plasmid, and recovering tannase from the culture; and a promoter represented by the nucleotide sequence of (SEQ ID NO:1). Tannase can be efficiently produced according to the present invention.

    摘要翻译： 本发明涉及含有编码鞣酸酶的基因并衍生自属于曲霉属的微生物的3,563个碱基对的DNA片段，其具有以下限制酶图：B：Bam HI，H：Hind III，K ：Kpn I，S：Sal I，X：Xba I; 含有编码（SEQ ID NO：4）的氨基酸序列的鞣酸酶基因的DNA片段; 包含插入质粒载体中的含有鞣酸酶基因的DNA片段的重组质粒; 一种鞣酸酶的制造方法，其特征在于，在培养基中培养能够在培养基中生产鞣酸酶的微生物属于属于曲霉属的微生物，从培养物中回收鞣酸酶; 和由（SEQ ID NO：1）的核苷酸序列表示的启动子。 根据本发明可以有效地制备鞣酸酶。