公开(公告)号：US07732570B2

公开(公告)日：2010-06-08

申请号：US11803694

申请日：2007-05-14

申请人： Paul R. Hinton ,  Naoya Tsurushita

发明人： Paul R. Hinton ,  Naoya Tsurushita

IPC分类号： C07K1/00 ,  C07K16/00 ,  C12P21/08 ,  A61K39/00

CPC分类号： C07K14/7151 ,  C07K14/5437 ,  C07K14/70528 ,  C07K16/00 ,  C07K2317/52 ,  C07K2319/30

摘要： The present invention provides for a modified Fc-fusion protein in which at least one amino acid from the heavy chain constant region selected from the group consisting of amino acid residues 250, 314, and 428 is substituted with another amino acid which is different from that present in the unmodified Fc-fusion protein, thereby altering the binding affinity for FcRn and/or the serum half-life in comparison to the unmodified Fc-fusion protein.

摘要翻译： 本发明提供了修饰的Fc融合蛋白，其中来自选自氨基酸残基250,314和428的重链恒定区的至少一个氨基酸被另一种不同于此的氨基酸取代 存在于未修饰的Fc融合蛋白中，从而与未修饰的Fc融合蛋白相比，改变了FcRn的结合亲和力和/或血清半衰期。

公开(公告)号：US07732195B2

公开(公告)日：2010-06-08

申请号：US11933819

申请日：2007-11-01

申请人： Yoshiko Akamatsu ,  Tsuneaki Asai ,  Naoya Tsurushita

发明人： Yoshiko Akamatsu ,  Tsuneaki Asai ,  Naoya Tsurushita

IPC分类号： C12N15/00 ,  C12N5/00 ,  G01N33/567

CPC分类号： C07K16/244 ,  C07K2317/23 ,  C07K2317/24 ,  C07K2317/622 ,  C07K2317/76

摘要： Disclosed are mammalian cell surface display vectors for isolating and/or characterizing immunoglobulins and various uses thereof.

摘要翻译： 公开了用于分离和/或表征免疫球蛋白的哺乳动物细胞表面展示载体及其各种用途。

公开(公告)号：US07666419B2

公开(公告)日：2010-02-23

申请号：US11581945

申请日：2006-10-16

申请人： William C. Olson ,  Paul J. Maddon ,  Naoya Tsurushita ,  Paul R. Hinton ,  Maximillano Vasquez

发明人： William C. Olson ,  Paul J. Maddon ,  Naoya Tsurushita ,  Paul R. Hinton ,  Maximillano Vasquez

IPC分类号： A61K39/395 ,  C12N15/13 ,  C07K16/28

CPC分类号： C07K16/2866 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/76

摘要： The invention is directed an anti-CCR5 antibody which comprises (i) two light chains, each light chain comprising the expression product of a plasmid designated pVK:HuPRO14O-VK (ATCC Deposit Designation PTA-4097), and (ii) two heavy chains, each heavy chain comprising an expression product of either a plasmid designated pVgl:HuPRO140 HG2-VH (ATCC Deposit Designation PTA-4098) or a plasmic designated pVgl:HuPRO140 (mutB+D+I)-VH (ATCC Deposit Designation PTA-4099) or a fragment thereof which binds to CCR5 on the surface of a human cell.

摘要翻译： 本发明涉及抗CCR5抗体，其包含（i）两条轻链，每条轻链包含命名为pVK：HuPRO14O-VK（ATCC保藏号PTA-4097）的质粒的表达产物，和（ii）两条重链 每个重链包含称为pVgl：HuPRO140HG2-VH（ATCC保藏号PTA-4098）或质粒命名为pVgl：HuPRO140（mutB + D + I）-VH（ATCC保藏号PTA-4099）的质粒的表达产物 ）或其与人细胞表面上的CCR5结合的片段。

公开(公告)号：US08969539B2

公开(公告)日：2015-03-03

申请号：US12456007

申请日：2009-06-10

申请人： Naoya Tsurushita ,  J. Yun Tso

发明人： Naoya Tsurushita ,  J. Yun Tso

IPC分类号： C07H21/04 ,  C12N15/13 ,  C12N15/63 ,  C12N15/85 ,  C12N5/10 ,  C07K16/00 ,  C07K16/24 ,  C07K16/28

CPC分类号： C07K16/00 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2267/01 ,  C07K16/241 ,  C07K16/2866 ,  C07K2319/03 ,  C07K2319/035 ,  C07K2319/30 ,  C07K2319/32 ,  C12N15/85 ,  C12N2840/44 ,  C12N2840/85

摘要： This present invention provides an expression vector system that uses alternative RNA processing to express in a single cell a polypeptide in both membrane-bound and soluble forms. By incorporating a mimetic structure of the 3′ terminal region of human mu gene and introducing other exogenous genetic elements, an artificial gene can be constructed that is capable of simultaneously expressing membrane-bound and secreted forms of polypeptides in myeloma cells and other cells of the B lymphocyte lineage, as well as in non-B cells. If an immunoglobulin heavy chain is co-expressed with a light chain using this vector, whole antibodies can be produced that are both displayed on the surface of a single cell and secreted into the cell culture supernatant. Membrane-bound antibodies facilitate isolation and expansion of those cells displaying antibodies with desired antigen binding characteristics, while secreted antibodies facilitate identification of antibodies having desired biological function(s).

摘要翻译： 本发明提供了一种表达载体系统，其使用替代的RNA加工来在单细胞中以膜结合和可溶形式表达多肽。 通过掺入人mu基因的3'末端区域的模拟结构并引入其他外源遗传元件，可以构建人造基因，其能够同时表达骨髓瘤细胞和其它细胞中的多肽的膜结合和分泌形式 B淋巴细胞谱系，以及非B细胞。 如果使用该载体使免疫球蛋白重链与轻链共同表达，则可以产生全部抗体，其均显示在单个细胞的表面并分泌到细胞培养上清液中。 膜结合的抗体促进显示具有所需抗原结合特征的抗体的那些细胞的分离和扩增，而分泌的抗体便于鉴定具有所需生物学功能的抗体。

公开(公告)号：US08624007B2

公开(公告)日：2014-01-07

申请号：US12751914

申请日：2010-03-31

申请人： Paul R. Hinton ,  Naoya Tsurushita

发明人： Paul R. Hinton ,  Naoya Tsurushita

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12P21/08

CPC分类号： C07K14/7151 ,  C07K14/5437 ,  C07K14/70528 ,  C07K16/00 ,  C07K2317/52 ,  C07K2319/30

摘要： The present invention provides for a modified Fc-fusion protein in which at least one amino acid from the heavy chain constant region selected from the group consisting of amino acid residues 250, 314, and 428 is substituted with another amino acid which is different from that present in the unmodified Fc-fusion protein, thereby altering the binding affinity for FcRn and/or the serum half-life in comparison to the unmodified Fc-fusion protein.

摘要翻译： 本发明提供了修饰的Fc融合蛋白，其中来自选自氨基酸残基250,314和428的重链恒定区的至少一个氨基酸被另一种不同于此的氨基酸取代 存在于未修饰的Fc融合蛋白中，从而与未修饰的Fc融合蛋白相比，改变了FcRn的结合亲和力和/或血清半衰期。

公开(公告)号：US08617829B2

公开(公告)日：2013-12-31

申请号：US13497692

申请日：2010-09-22

申请人： Shankar Kumar ,  J. Yun Tso ,  Naoya Tsurushita

发明人： Shankar Kumar ,  J. Yun Tso ,  Naoya Tsurushita

IPC分类号： C12Q1/68 ,  C07K16/00 ,  C07K16/46 ,  C07K16/24

CPC分类号： C07K16/18 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/76

摘要： The present invention provides humanized antibodies that immunospecifically recognize the RGD sequence. Some of these antibodies inhibit the biological functions of the RGD proteins, thereby exhibiting therapeutic effects on various disorders or diseases that are associated with RGD proteins, including cancer, e.g., the growth and metastasis of a cancer cell, and inflammatory diseases, e.g., rheumatoid arthritis, osteoarthritis, hepatitis, endometriosis, bronchial asthma, fibrosis, diabetes, arteriosclerosis, multiple sclerosis, granuloma, an inflammatory bowel disease (ulcerative colitis and Crohn's disease), an autoimmune disease, and so forth.

公开(公告)号：US08163546B2

公开(公告)日：2012-04-24

申请号：US12748247

申请日：2010-03-26

申请人： Yoshiko Akamatsu ,  Tsuneaki Asai ,  Naoya Tsurushita

发明人： Yoshiko Akamatsu ,  Tsuneaki Asai ,  Naoya Tsurushita

IPC分类号： C12N15/00 ,  C12N5/00 ,  G01N33/567

CPC分类号： C07K16/244 ,  C07K2317/23 ,  C07K2317/24 ,  C07K2317/622 ,  C07K2317/76

摘要： Disclosed are mammalian cell surface display vectors for isolating and/or characterizing immunoglobulins and various uses thereof.

摘要翻译： 公开了用于分离和/或表征免疫球蛋白的哺乳动物细胞表面展示载体及其各种用途。

公开(公告)号：US08017118B2

公开(公告)日：2011-09-13

申请号：US12404419

申请日：2009-03-16

申请人： Koji Nakamura ,  Rie Tajima ,  Shankar Kumar ,  J. Yun Tso ,  Naoya Tsurushita

发明人： Koji Nakamura ,  Rie Tajima ,  Shankar Kumar ,  J. Yun Tso ,  Naoya Tsurushita

IPC分类号： C07K16/18

CPC分类号： C07K16/30 ,  A61K2039/505 ,  C07K16/28 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/73 ,  C07K2317/732 ,  C07K2317/77 ,  C07K2317/92 ,  G01N2800/00

摘要： The present invention provides antibodies specifically against hDlk-1 and having anti-tumor activity in vivo (anti-hDlk-1 antibodies), a fragments of the antibodies, hybridomas that produce the antibodies, a complex of the antibody or antibody fragment and an agent, a pharmaceutical composition comprising the antibody and the like, a tumor therapeutic agent, a tumor angiogenesis inhibitor, a tumor diagnostic agent, a method for detecting tumor, a kit for detecting and/or diagnosing tumor, etc.

摘要翻译： 本发明提供抗体特异性抗体，并具有体内抗肿瘤活性（抗hDlk-1抗体），抗体片段，产生抗体的杂交瘤，抗体或抗体片段的复合物和药剂 ，包含抗体等的药物组合物，肿瘤治疗剂，肿瘤血管生成抑制剂，肿瘤诊断剂，肿瘤检测方法，肿瘤检测和/或诊断用试剂盒等。

公开(公告)号：US20110183412A1

公开(公告)日：2011-07-28

申请号：US12751914

申请日：2010-03-31

申请人： Paul R. Hinton ,  Naoya Tsurushita

发明人： Paul R. Hinton ,  Naoya Tsurushita

IPC分类号： C12N15/63 ,  C07H21/00

CPC分类号： C07K14/7151 ,  C07K14/5437 ,  C07K14/70528 ,  C07K16/00 ,  C07K2317/52 ,  C07K2319/30

摘要： The present invention provides for a modified Fc-fusion protein in which at least one amino acid from the heavy chain constant region selected from the group consisting of amino acid residues 250, 314, and 428 is substituted with another amino acid which is different from that present in the unmodified Fc-fusion protein, thereby altering the binding affinity for FcRn and/or the serum half-life in comparison to the unmodified Fc-fusion protein.

摘要翻译： 本发明提供了修饰的Fc融合蛋白，其中来自选自氨基酸残基250,314和428的重链恒定区的至少一个氨基酸被另一种不同于此的氨基酸取代 存在于未修饰的Fc融合蛋白中，从而与未修饰的Fc融合蛋白相比，改变了FcRn的结合亲和力和/或血清半衰期。

公开(公告)号：US20080287657A1

公开(公告)日：2008-11-20

申请号：US11803694

申请日：2007-05-14

申请人： Paul R. Hinton ,  Naoya Tsurushita

发明人： Paul R. Hinton ,  Naoya Tsurushita

IPC分类号： C07K16/18

CPC分类号： C07K14/7151 ,  C07K14/5437 ,  C07K14/70528 ,  C07K16/00 ,  C07K2317/52 ,  C07K2319/30

摘要： The present invention provides for a modified Fc-fusion protein in which at least one amino acid from the heavy chain constant region selected from the group consisting of amino acid residues 250, 314, and 428 is substituted with another amino acid which is different from that present in the unmodified Fc-fusion protein, thereby altering the binding affinity for FcRn and/or the serum half-life in comparison to the unmodified Fc-fusion protein.

摘要翻译： 本发明提供了修饰的Fc融合蛋白，其中来自选自氨基酸残基250,314和428的重链恒定区的至少一个氨基酸被另一种不同于此的氨基酸取代 存在于未修饰的Fc融合蛋白中，从而与未修饰的Fc融合蛋白相比，改变了FcRn的结合亲和力和/或血清半衰期。