公开(公告)号：US11981908B2

公开(公告)日：2024-05-14

申请号：US17575318

申请日：2022-01-13

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flasinski ,  Arlene R. Howe ,  Krishnakumar Sridharan

IPC: C12N15/82 ,  A01N37/46 ,  A01N63/50 ,  C07K14/195 ,  C07K14/32

CPC classification number: C12N15/8286 ,  A01N37/46 ,  A01N63/50 ,  C07K14/195 ,  C07K14/32 ,  C07K2319/00 ,  A01N63/50 ,  A01N63/20

Abstract: A pesticidal protein class exhibiting toxic activity against Coleopteran and Lepidopteran pest species is disclosed, and includes, but is not limited to, TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran and Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins are also provided.

公开(公告)号：US11981902B2

公开(公告)日：2024-05-14

申请号：US17165043

申请日：2021-02-02

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski ,  Charles R. Dietrich ,  Wei Wu ,  Zhaolong Li ,  Bo-Xing Qiu ,  Liang Guo ,  Jaishree M. Chittoor

IPC: C12N15/82 ,  C12N15/113

CPC classification number: C12N15/8221 ,  C12N15/113 ,  C12N15/8222

Abstract: The invention provides DNA molecules and constructs, and their nucleotide sequences, useful for modulating gene expression in plants, and for specifying intracellular or extracellular localization of a gene product of interest. Transgenic plants, plant cells, plant parts, and seeds, comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided.

公开(公告)号：US11913010B2

公开(公告)日：2024-02-27

申请号：US17568547

申请日：2022-01-04

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： David J. Bowen ,  Catherine Chay ,  Artem Evdokimov ,  Stanislaw Flasinski ,  Uma R. Kesanapalli ,  Megan N. Schroder ,  Rachael N. Slightom ,  Nengbing Tao ,  Andrew M. Wollacott

IPC: C12N15/82 ,  A01N65/04 ,  C07K14/32

CPC classification number: C12N15/8286 ,  A01N65/04 ,  C07K14/32 ,  Y02A40/146

Abstract: The present invention discloses a genus of insect inhibitory proteins that exhibit properties directed to controlling Lepidopteran and/or Hemipteran crop pests, methods of using such proteins, nucleotide sequences encoding such proteins, methods of detecting and isolating such proteins, and their use in agricultural systems.

公开(公告)号：US11512324B2

公开(公告)日：2022-11-29

申请号：US16580583

申请日：2019-09-24

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flasinski ,  Arlene R. Howe

IPC: C12N15/82 ,  C07K14/325 ,  A01N63/50 ,  G01N33/569 ,  G01N33/00 ,  A01N63/10

Abstract: A pesticidal protein class of PirA, PirB, and PirAB fusion proteins exhibiting toxic activity against Coleopteran, Lepidopteran, and Hemipteran pest species is disclosed. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the PirA, PirB, and PirAB fusion proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran, Lepidopteran, and Hemipteran infestation are provided which contain recombinant nucleic acid sequences encoding the PirA, PirB, and PirAB fusion proteins. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran, Lepidopteran, and Hemipteran species pests using the PirA, PirB, and PirAB fusion proteins are also provided.

公开(公告)号：US20220306703A1

公开(公告)日：2022-09-29

申请号：US17671011

申请日：2022-02-14

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Thomas A. Cerruti ,  Crystal L. Dart ,  Leigh H. English ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Victor M. Guzov ,  Arlene R. Howe ,  Jay P. Morgenstern ,  James K. Roberts ,  Sara A. Salvador ,  Jinling Wang

IPC: C07K14/325 ,  C12N15/82

Abstract: Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.

公开(公告)号：US20210310015A1

公开(公告)日：2021-10-07

申请号：US17229604

申请日：2021-04-13

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski ,  Barrett C. Foat ,  Mohammed Oufattole ,  Randall W. Shultz ,  Xiaoping Wei ,  Wei Wu ,  Shiaw-Pyng Yang

IPC: C12N15/82

Abstract: The invention provides DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. Transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided, as are methods of their use.

公开(公告)号：US20210230621A1

公开(公告)日：2021-07-29

申请号：US17165055

申请日：2021-02-02

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski ,  Charles R. Dietrich ,  Wei Wu ,  Zhaolong Li ,  Bo-Xing Qiu ,  Liang Guo ,  Jaishree M. Chittoor

IPC: C12N15/82 ,  C12N15/113

Abstract: The invention provides DNA molecules and constructs, and their nucleotide sequences, useful for modulating gene expression in plants, and for specifying intracellular or extracellular localization of a gene product of interest. Transgenic plants, plant cells, plant parts, and seeds, comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided.

公开(公告)号：US11053510B2

公开(公告)日：2021-07-06

申请号：US16563801

申请日：2019-09-06

Applicant: Monsanto Technology LLC

Inventor： Jaishree M. Chittoor ,  Stanislaw Flasinski ,  Mohammed Oufattole ,  Michael W. Petersen

IPC: C12N15/82 ,  C07K14/415

Abstract: The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.

公开(公告)号：US10731170B2

公开(公告)日：2020-08-04

申请号：US15179635

申请日：2016-06-10

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski

IPC: C12N15/82 ,  C12N15/113

Abstract: The present invention provides novel DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides, along with methods of their use.

公开(公告)号：US10717989B2

公开(公告)日：2020-07-21

申请号：US15846796

申请日：2017-12-19

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flasinski ,  Arlene R. Howe ,  Jason S. Milligan ,  James K. Roberts ,  Yong Yin

IPC: C12N15/82

Abstract: Pesticidal proteins exhibiting toxic activity against Coleopteran, Lepidopteran, Hemipteran, and Thysanopteran pest species are disclosed, and include, but are not limited to, TIC6280, TIC6281, TIC6282, TIC6283, TIC8808, TIC9480, TIC9257, TIC7106, TIC7017, TIC7107, TIC7108, TIC7109, TIC7110, TIC7111, TIC7589, TIC9258, and TIC9259. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the pesticidal proteins provided. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran, Coleopteran, Hemipteran and Thysanopteran infestation are provided which contain recombinant nucleic acid sequences encoding the disclosed pesticidal proteins. Methods for detecting the presence of the recombinant nucleic acid sequences or the protein of the present invention in a biological sample, and methods of controlling Coleopteran, Lepidopteran, Hemipteran, and Thysanopteran species pests using the disclosed pesticidal proteins are also provided.