公开(公告)号：US20180312867A1

公开(公告)日：2018-11-01

申请号：US15979061

申请日：2018-05-14

Applicant: Monsanto Technology LLC

Inventor： Andrey A. Boukharov ,  Zijin Du ,  Liang Guo ,  Michelle C. Hresko ,  David K. Kovalic ,  Zhaolong Li ,  Maolong Lu ,  James P. McCarter ,  Nancy M. Miller ,  Mark Vaudin ,  Deryck J. Williams ,  Wei Wu

IPC: C12N15/82 ,  C12Q1/6888 ,  C07K14/435 ,  A01N57/16 ,  A61K31/713 ,  C12N15/113 ,  A23L33/105

Abstract: The invention relates to identifying and evaluating target coding sequences for control of plant parasitic nematodes by inhibiting one or more biological functions, and their use. The invention provides methods and compositions for identification of such sequences and for the control of a plant-parasitic nematode population. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the nematode, a reduction in disease may be obtained through suppression of nematode gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and the plant cells and plants obtained thereby.

公开(公告)号：US20230041449A1

公开(公告)日：2023-02-09

申请号：US17066053

申请日：2020-10-08

Applicant: Monsanto Technology LLC

Inventor： Liang Guo ,  David Kovalic ,  Bo-Xing Qiu ,  Jack Tabaska ,  Wei Wu

IPC: C12N15/82 ,  C07K14/415

Abstract: This disclosure provides purified nucleic acids and polypeptides. Also provided are transgenic plants, seeds, and plant cells containing DNA for expression of the proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants, methods of making such plants and methods of making agricultural commodity including seeds and hybrid seeds from such plants.

公开(公告)号：US10435686B2

公开(公告)日：2019-10-08

申请号：US14567786

申请日：2014-12-11

Applicant: Monsanto Technology LLC

Inventor： Edwards M. Allen ,  Barry S. Goldman ,  Liang Guo ,  Sara E. Heisel ,  Shihshieh Huang ,  Sergey I. Ivashuta ,  David K. Kovalic ,  Elysia K. Krieger ,  James K. Roberts ,  Yuanji Zhang

IPC: C12N15/113 ,  C12N15/82

Abstract: This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.

公开(公告)号：US20170152521A9

公开(公告)日：2017-06-01

申请号：US14685063

申请日：2015-04-13

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Wei Wu ,  Jack Tabaska ,  David Kovalic ,  Bo-Xing Qiu ,  Liang Guo

IPC: C12N15/82

CPC classification number: C12N15/8218 ,  A01H5/10 ,  C07K14/415 ,  C12N15/8273 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8278

Abstract: This disclosure provides purified nucleic acids and polypeptides. Also provided are transgenic plants, seeds, and plant cells containing DNA for expression of the proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants, methods of making such plants and methods of making agricultural commodity including seeds and hybrid seeds from such plants.

公开(公告)号：US20160168569A1

公开(公告)日：2016-06-16

申请号：US15052062

申请日：2016-02-24

Applicant: Monsanto Technology LLC

Inventor： Edwards Allen ,  Liang Guo ,  Sara E. Heisel ,  Sergey I. Ivashuta ,  Yuanji Zhang

IPC: C12N15/113 ,  C12N15/82

CPC classification number: C12N15/113 ,  C12N15/8218 ,  C12N15/8245 ,  C12N15/8254 ,  C12N15/8266 ,  C12N15/827

Abstract: This invention discloses recombinant DNA constructs encoding phased small RNAs useful in regulating expression of one or more genes of interest. Also disclosed by this invention are transgenic plant cells, plants, and seeds containing a recombinant DNA construct of this invention.

Abstract translation: 本发明公开了编码可用于调节一种或多种感兴趣的基因表达的分阶段的小RNA的重组DNA构建体。 本发明还公开了含有本发明的重组DNA构建体的转基因植物细胞，植物和种子。

公开(公告)号：US09309512B2

公开(公告)日：2016-04-12

申请号：US13775477

申请日：2013-02-25

Applicant: Monsanto Technology LLC

Inventor： Edwards Allen ,  Liang Guo ,  Sara E. Heisel ,  Sergey I. Ivashuta ,  Yuanji Zhang

IPC: C12N15/82 ,  A01H1/00 ,  C07H21/04 ,  C12N15/113

CPC classification number: C12N15/113 ,  C12N15/8218 ,  C12N15/8245 ,  C12N15/8254 ,  C12N15/8266 ,  C12N15/827

Abstract: This invention discloses recombinant DNA constructs encoding phased small RNAs useful in regulating expression of one or more genes of interest. Also disclosed by this invention are transgenic plant cells, plants, and seeds containing a recombinant DNA construct of this invention.

公开(公告)号：US20240043856A1

公开(公告)日：2024-02-08

申请号：US18460371

申请日：2023-09-01

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski ,  Charles R. Dietrich ,  Wei Wu ,  Zhaolong Li ,  Bo-Xing Qiu ,  Liang Guo ,  Jaishree M. Chittoor

IPC: C12N15/82 ,  C12N15/113

CPC classification number: C12N15/8221 ,  C12N15/113 ,  C12N15/8222

Abstract: The invention provides DNA molecules and constructs, and their nucleotide sequences, useful for modulating gene expression in plants, and for specifying intracellular or extracellular localization of a gene product of interest. Transgenic plants, plant cells, plant parts, and seeds, comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided.

公开(公告)号：US20210230620A1

公开(公告)日：2021-07-29

申请号：US17165043

申请日：2021-02-02

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski ,  Charles R. Dietrich ,  Wei Wu ,  Zhaolong Li ,  Bo-Xing Qiu ,  Liang Guo ,  Jaishree M. Chittoor

IPC: C12N15/82 ,  C12N15/113

Abstract: The invention provides DNA molecules and constructs, and their nucleotide sequences, useful for modulating gene expression in plants, and for specifying intracellular or extracellular localization of a gene product of interest. Transgenic plants, plant cells, plant parts, and seeds, comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided.

公开(公告)号：US10995340B2

公开(公告)日：2021-05-04

申请号：US16374211

申请日：2019-04-03

Applicant: Monsanto Technology LLC

Inventor： Stanislaw Flasinski ,  Charles R. Dietrich ,  Wei Wu ,  Zhaolong Li ,  Bo-Xing Qiu ,  Liang Guo ,  Jaishree M. Chittoor

IPC: C12N15/82 ,  C12N15/113

Abstract: The invention provides DNA molecules and constructs, and their nucleotide sequences, useful for modulating gene expression in plants, and for specifying intracellular or extracellular localization of a gene product of interest. Transgenic plants, plant cells, plant parts, and seeds, comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided.

公开(公告)号：US10829781B2

公开(公告)日：2020-11-10

申请号：US15979061

申请日：2018-05-14

Applicant: Monsanto Technology LLC

Inventor： Andrey A. Boukharov ,  Zijin Du ,  Liang Guo ,  Michelle C. Hresko ,  David K. Kovalic ,  Zhaolong Li ,  Maolong Lu ,  James P. McCarter ,  Nancy M. Miller ,  Mark Vaudin ,  Deryck J. Williams ,  Wei Wu

IPC: C12N15/82 ,  C07K14/435 ,  A61K31/713 ,  C12N15/113 ,  A23L33/105 ,  A01N57/16 ,  C12Q1/6888

Abstract: The invention relates to identifying and evaluating target coding sequences for control of plant parasitic nematodes by inhibiting one or more biological functions, and their use. The invention provides methods and compositions for identification of such sequences and for the control of a plant-parasitic nematode population. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the nematode, a reduction in disease may be obtained through suppression of nematode gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and the plant cells and plants obtained thereby.