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公开(公告)号:US09902946B2
公开(公告)日:2018-02-27
申请号:US14759068
申请日:2013-12-20
申请人: NOVOZYMES A/S
发明人: Carsten Andersen
CPC分类号: C12N9/2414 , C11D3/386 , C12N9/2417 , C12Y302/01001
摘要: The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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公开(公告)号:US20170121695A1
公开(公告)日:2017-05-04
申请号:US15317823
申请日:2015-06-12
申请人: NOVOZYMES A/S
发明人: Carsten Andersen , Chakshusmathi Ghadiyaram , Rajendra Kulothungan Sainathan , Padmavathi Balumuri , Padma Venkatachalam Iyer , Iben Damager , Astrid Munch , Subith Krishna
CPC分类号: C12N9/2417 , C11D1/22 , C11D3/386 , C11D3/38618 , C11D11/0017 , C12Y302/01001
摘要: The present invention relates to variants having alpha-amylase activity and polynucleotides encoding the variants. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, compositions comprising the variants, as well as methods of producing and using the variants.
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公开(公告)号:US20170096650A1
公开(公告)日:2017-04-06
申请号:US15302062
申请日:2015-04-10
申请人: Novozymes A/S
发明人: Carsten Andersen , Paria Saunders
CPC分类号: C12N9/2417 , A21D8/042 , C11D3/386 , C12C5/004 , C12C2200/05 , C12P7/06 , C12P19/02 , C12P19/14 , C12P2203/00 , C12Y302/01001 , D06L1/14 , Y02E50/17
摘要: The present invention relates to alpha-amylase variants with improved stability in the presence of glucose and/or relatively higher activity on long chain versus short chain substrates. The present encoding invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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公开(公告)号:US09464279B2
公开(公告)日:2016-10-11
申请号:US14330747
申请日:2014-07-14
申请人: Novozymes A/S
发明人: Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgaard-Frantzen , Allan Svendsen , Soren Kjaerulff
CPC分类号: C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
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公开(公告)号:US20160222365A1
公开(公告)日:2016-08-04
申请号:US15012229
申请日:2016-02-01
申请人: NOVOZYMES A/S
发明人: Torben Vedel Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Lauesgaard Nissen , Soren Kjaerulff
IPC分类号: C12N9/28
摘要: The invention relates to a variant of a parent Termamyl-like α-amylase, which exhibits an alteration in at least one of the following properties relative to said parent α-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.
摘要翻译: 本发明涉及相对于所述亲本α-淀粉酶显示出至少一种以下特性的母体类戊酰样α-淀粉酶的变体:i)在8至10.5的pH下改善的pH稳定性; 和/或ii)在pH8至10.5时提高的Ca 2+稳定性,和/或iii)在10至60℃的温度下提高比活性。
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公开(公告)号:US20160053244A1
公开(公告)日:2016-02-25
申请号:US14755048
申请日:2015-06-30
申请人: NOVOZYMES A/S
IPC分类号: C12N9/28
CPC分类号: C12N9/2417 , C07K2299/00 , C12Y302/01001
摘要: The present invention relates to variants (mutants) of polypeptides, in particular Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency, specific activity, in particular laundry and dish-wash applications.
摘要翻译: 本发明涉及多肽的变体(突变体),特别是类戊酰样α-淀粉酶,该变体具有α-淀粉酶活性并且相对于所述亲本α-淀粉酶在至少一个以下性质中具有改变:底物特异性 ,底物结合,底物切割模式,热稳定性,pH /活性分布,pH /稳定性分布,对氧化的稳定性,Ca 2+依赖性,比活性,特别是洗衣和洗碗应用。
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公开(公告)号:US20150044732A1
公开(公告)日:2015-02-12
申请号:US14525877
申请日:2014-10-28
申请人: NOVOZYMES A/S
发明人: Carsten Andersen
CPC分类号: C12N9/2417 , C11D3/386 , C11D3/38681 , C12N9/2414 , C12N15/8257 , C12P7/06 , C12P7/14 , C12P19/02 , C12P19/04 , C12P19/14 , C12P2203/00 , C12Y302/01001 , Y02E50/17
摘要: The present invention relates to variants of a parent alpha-amylase, the variant having improved stability or activity at low calcium conditions or at low pH.
摘要翻译: 本发明涉及母体α-淀粉酶的变体,该变体在低钙条件下或在低pH下具有改善的稳定性或活性。
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公开(公告)号:US20150024084A1
公开(公告)日:2015-01-22
申请号:US14504517
申请日:2014-10-02
申请人: NOVOZYMES A/S
CPC分类号: C12N9/16 , A23K20/189 , C12Y301/03
摘要: The present invention relates to a phytase which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least one alteration as compared to this phytase. These phytase variants have amended, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an amended glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.
摘要翻译: 本发明涉及一种植酸酶,其与来自柠檬酸杆菌的植酸酶具有至少74%的同一性,并且与该植酸酶相比包含至少一个改变。 这些植酸酶变体具有修饰,优选改进的性质,例如热稳定性,温度曲线,pH曲线,比活性,动物饲料中的性能,降低的蛋白酶敏感性和/或修饰的糖基化模式。 本发明还涉及编码这些植酸酶的DNA,其生产方法及其用途,例如在动物饲料和动物饲料添加剂中。
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公开(公告)号:US20140356924A1
公开(公告)日:2014-12-04
申请号:US14467740
申请日:2014-08-25
申请人: NOVOZYMES A/S
CPC分类号: C12N9/2417 , C11D3/386 , C11D3/38618 , C12P7/06 , C12P7/14 , C12P19/14 , C12Y302/01001 , Y02E50/17
摘要: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.
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公开(公告)号:US20140030767A1
公开(公告)日:2014-01-30
申请号:US13907024
申请日:2013-05-31
申请人: Novozymes A/S
CPC分类号: C12N9/2417 , C11D3/386 , C11D3/38618 , C11D3/38681 , C12P7/14 , C12P19/02 , C12P19/14 , C12Y302/01001 , D06M16/003 , Y02E50/17
摘要: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca2+ to the parent alpha-amylase.
摘要翻译: 本发明涉及母体类戊酰样α-淀粉酶的变体(突变体),该变体具有α-淀粉酶活性并且表现出改变的稳定性,特别是在高温和/或低pH相对和/或低Ca 2+下 亲本α-淀粉酶。
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