公开(公告)号：US20180169403A1

公开(公告)日：2018-06-21

申请号：US15542197

申请日：2016-01-08

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Hongkun Park

IPC: A61N1/05 ,  A61N1/06 ,  H01L23/49 ,  B82Y5/00

CPC classification number: A61N1/0531 ,  A61N1/05 ,  A61N1/0536 ,  A61N1/06 ,  B82Y5/00 ,  H01L23/48 ,  H01L23/49 ,  H01L29/0676 ,  H01L2924/0002 ,  H01L2924/10253 ,  H01L2924/14 ,  H01L2924/00

Abstract: The present invention generally relates to nanowires. In one aspect, the present invention is generally directed to systems and methods of individually addressing nanowires on a surface, e.g., that are substantially upstanding or vertically-oriented with respect to the surface. In some cases, one or more nanowires may be individually addressed using various integrated circuit (“IC”) technologies, such as CMOS. For example, the nanowires may form an array on top of an active CMOs integrated circuit.

公开(公告)号：US20170349950A1

公开(公告)日：2017-12-07

申请号：US15687089

申请日：2017-08-25

Applicant: THE BROAD INSTITUTE INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  THE BRIGHAM AND WOMEN'S HOSPITAL, INC. ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aviv Regev ,  Vijay K. Kuchroo ,  Jellert Gaublomme ,  Youjin Lee ,  Chao Wang ,  Nir Yosef ,  Hongkun Park ,  James Kaminski

IPC: C12Q1/68 ,  G01N33/50

CPC classification number: C12Q1/6883 ,  C12Q1/6881 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/158 ,  G01N33/5023

Abstract: This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

公开(公告)号：US20160281110A1

公开(公告)日：2016-09-29

申请号：US15051418

申请日：2016-02-23

Applicant: President and Fellows of Harvard College

Inventor： Hongkun Park ,  EunGyeong Yang ,  Alexander K. Shalek ,  JinSeok Lee ,  Jacob Robinson ,  Amy Sutton ,  Myung-Han Yoon ,  Marsela Jorgolli

IPC: C12N15/89 ,  G01N33/50 ,  C12Q1/68 ,  C12N5/00

CPC classification number: C12N15/89 ,  C12N5/0075 ,  C12N2533/00 ,  C12N2535/00 ,  C12Q1/68 ,  G01N33/5008 ,  G01N33/5047 ,  G01N33/54353

Abstract: A molecular delivery system including a plurality of nanowires (e.g., Si NWs), each of the nanowires having a surface layer formed of a silicon-containing material and a covalently bound linker (e.g., silane linker) attached to the surface layer and optionally including a substrate to which the nanowires are adhered or a molecule to be delivered attached to the linker. Also disclosed is a method of delivering into a cell an exogenous molecule.

Abstract translation: 包括多个纳米线（例如，Si NW）的分子递送系统，每个纳米线具有由含硅材料形成的表面层和附着到表面层的共价结合的接头（例如，硅烷接头），并且任选地包括 纳米线所粘附的基底或待连接到接头上的分子。 还公开了向细胞递送外源性分子的方法。

公开(公告)号：US20150197807A1

公开(公告)日：2015-07-16

申请号：US14422497

申请日：2013-03-15

Applicant: President and Fellows of Harvard College

Inventor： Hongkun Park ,  Alexander K. Shalek ,  Jellert T. Gaublomme

IPC: C12Q1/68 ,  G01N33/50

CPC classification number: C12Q1/6883 ,  B82Y5/00 ,  B82Y15/00 ,  C12N15/111 ,  C12N15/87 ,  C12N2310/14 ,  C12N2310/351 ,  C12N2320/32 ,  C12N2330/31 ,  C12Q2600/158 ,  G01N33/502 ,  G01N33/5047 ,  G01N33/54346 ,  G01N33/54373 ,  G01N2333/52 ,  G01N2500/04 ,  G01N2500/10

Abstract: The present invention generally relates to nanowires and, in some aspects, to methods of using nanowire arrays to identify a therapeutic target for treating a disorder in a subject, identify a treatment for a disorder in a subject, or deliver a biological effector to immune cells. Previous techniques for delivering biological effectors into live immune cells yielded low efficiencies, activated the immune response and induced non-specific inflammation, and/or required harsh conditions that resulted in widespread apoptosis. By contrast, some of the methods described herein are capable of efficiently delivering biomolecular cargo into immune cells, have negligible toxicity, do not activate immune cell function, and/or allow cells to respond appropriately to physiological stimuli.

Abstract translation: 本发明通常涉及纳米线，并且在一些方面涉及使用纳米线阵列来鉴定用于治疗受试者中的病症的治疗靶标，识别受试者中的病症的治疗或将免疫细胞递送生物效应物的方法 。 用于将生物效应物递送到活的免疫细胞中的以前的技术产生低效率，激活免疫应答并诱导非特异性炎症和/或导致广泛凋亡的所需恶劣条件。 相比之下，本文描述的一些方法能够有效地将生物分子货物输送到免疫细胞中，具有可忽略的毒性，不激活免疫细胞功能和/或允许细胞适当地对生理刺激作出反应。