公开(公告)号：US11427869B2

公开(公告)日：2022-08-30

申请号：US15687089

申请日：2017-08-25

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  THE BRIGHAM AND WOMEN'S HOSPITAL, INC. ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Aviv Regev ,  Vijay K. Kuchroo ,  Jellert Gaublomme ,  Youjin Lee ,  Chao Wang ,  Nir Yosef ,  Hongkun Park ,  James Kaminski

IPC分类号： C12Q1/6883 ,  C12Q1/6881 ,  G01N33/50

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

公开(公告)号：US20170349950A1

公开(公告)日：2017-12-07

申请号：US15687089

申请日：2017-08-25

申请人： THE BROAD INSTITUTE INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  THE BRIGHAM AND WOMEN'S HOSPITAL, INC. ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Aviv Regev ,  Vijay K. Kuchroo ,  Jellert Gaublomme ,  Youjin Lee ,  Chao Wang ,  Nir Yosef ,  Hongkun Park ,  James Kaminski

IPC分类号： C12Q1/68 ,  G01N33/50

CPC分类号： C12Q1/6883 ,  C12Q1/6881 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/158 ,  G01N33/5023

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

公开(公告)号：US11209440B2

公开(公告)日：2021-12-28

申请号：US15245748

申请日：2016-08-24

申请人： THE BROAD INSTITUTE, INC. ,  THE BRIGHAM AND WOMEN'S HOSPITAL, INC. ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Vijay Kuchroo ,  Aviv Regev ,  Jellert Gaublomme ,  Youjin Lee ,  Alexander K. Shalek ,  Chao Wang ,  Nir Yosef ,  Hongkun Park

IPC分类号： G01N33/68 ,  C12Q1/6883 ,  A61K48/00 ,  C12N15/68 ,  C12N15/86 ,  C07K16/28

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

公开(公告)号：US10822587B2

公开(公告)日：2020-11-03

申请号：US14837702

申请日：2015-08-27

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College ,  The Brigham and Women's Hospital, Inc.

发明人： Aviv Regev ,  Vijay Kuchroo ,  Hongkun Park ,  Nir Yosef ,  Alexander K. Shalek ,  Jellert Gaublomme ,  Nicole C. Joller ,  Chuan Wu ,  Ana Carrizosa Anderson

IPC分类号： C12N5/071 ,  C12N5/00 ,  C12N5/02 ,  C07H21/04 ,  C12N5/0783 ,  C12Q1/6881 ,  A61K31/12 ,  A61K31/198 ,  A61K31/37 ,  A61K31/4745 ,  A61K31/66 ,  A61K31/661 ,  A61K31/7076 ,  A61K31/7088 ,  A61K31/739 ,  A61K33/00 ,  A61K33/06 ,  A61K33/24 ,  A61K38/17 ,  A61K38/18 ,  A61K38/19 ,  A61K38/36 ,  A61K38/48 ,  C07K16/28 ,  C12N15/113 ,  G01N33/569 ,  A61K48/00

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

公开(公告)号：US20150361396A1

公开(公告)日：2015-12-17

申请号：US14837702

申请日：2015-08-27

申请人： THE BROAD INSTITUTE INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  THE BRIGHAM AND WOMEN'S HOSPITAL, INC.

发明人： Aviv Regev ,  Vijay Kuchroo ,  Hongkun Park ,  Nir Yosef ,  Alexander K. Shalek ,  Jellert Gaublomme ,  Nicole C. Joller ,  Chuan Wu ,  Ana Carrizosa Anderson

IPC分类号： C12N5/0783 ,  A61K31/739 ,  A61K33/24 ,  A61K38/36 ,  A61K31/4745 ,  A61K31/7076 ,  A61K31/198 ,  A61K38/17 ,  A61K31/66 ,  A61K38/48 ,  A61K38/18 ,  A61K31/661 ,  A61K33/06 ,  A61K31/37 ,  A61K31/12 ,  A61K33/00 ,  C07K16/28 ,  A61K38/19 ,  C12Q1/68 ,  G01N33/569 ,  C12N15/113 ,  A61K31/7088

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

摘要翻译： 本发明一般涉及用于鉴定调节，控制或以其他方式影响T细胞平衡的调节网络的组合物和方法，例如Th17细胞分化，维持和/或功能，以及用于开发调节网络的调节网络的组合物和方法， 控制或以其他方式影响各种治疗和/或诊断适应症中的T细胞平衡。 本发明还涉及鉴定和利用调节，控制或以其他方式影响多种治疗和/或诊断适应症中的T细胞平衡的靶基因和/或靶基因产物。

公开(公告)号：US20200081017A1

公开(公告)日：2020-03-12

申请号：US16675398

申请日：2019-11-06

申请人： The Brigham and Women's Hospital, Inc. ,  The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

发明人： Vijay K. Kuchroo ,  Aviv Regev ,  Jellert Gaublomme ,  Youjin Lee ,  Alexander K. Shalek ,  Chao Wang ,  Nir Yosef ,  Hongkun Park

IPC分类号： G01N33/68 ,  C12Q1/6883 ,  C07K16/28

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

公开(公告)号：US20190085324A1

公开(公告)日：2019-03-21

申请号：US15965192

申请日：2018-04-27

申请人： The Broad Institute Inc. ,  Massachusetts Institute of Technology ,  The Regents of the University of California

发明人： Aviv Regev ,  Brittany Adamson ,  Brian Cleary ,  Le Cong ,  Atray Dixit ,  Jellert Gaublomme ,  Eric S. Lander ,  Thomas Norman ,  Oren Parnas ,  Orit Rozenblatt-Rosen ,  Alexander K. Shalek ,  Jonathan Weissman

IPC分类号： C12N15/10 ,  C12N15/11 ,  C12N9/22 ,  C12N15/113 ,  C12Q1/6886

摘要： The present invention provides tools and methods for the systematic analysis of genetic interactions, including higher order interactions. The present invention provides tools and methods for combinatorial probing of cellular circuits, for dissecting cellular circuitry, for delineating molecular pathways, and/or for identifying relevant targets for therapeutics development.

公开(公告)号：US11214797B2

公开(公告)日：2022-01-04

申请号：US15965192

申请日：2018-04-27

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  The Regents of the University of California

发明人： Aviv Regev ,  Brittany Adamson ,  Brian Cleary ,  Le Cong ,  Atray Dixit ,  Jellert Gaublomme ,  Eric S. Lander ,  Thomas Norman ,  Oren Parnas ,  Orit Rozenblatt-Rosen ,  Alexander K. Shalek ,  Jonathan Weissman

IPC分类号： C12N15/10 ,  G16B5/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C12Q1/6886

摘要： The present invention provides tools and methods for the systematic analysis of genetic interactions, including higher order interactions. The present invention provides tools and methods for combinatorial probing of cellular circuits, for dissecting cellular circuitry, for delineating molecular pathways, and/or for identifying relevant targets for therapeutics development.

公开(公告)号：US20190285644A1

公开(公告)日：2019-09-19

申请号：US16352355

申请日：2019-03-13

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Aviv Regev ,  Jellert Gaublomme

IPC分类号： G01N33/68 ,  C12N15/10

摘要： The application relates to methods and systems for proteomics and spatial mapping of biomolecules using a next generation sequencing readout to decipher biomolecular and cellular interaction networks. Specifically, disclosed are antenna networks generated by conjugating DNA antennas to proteins. The antennas carry a unique antenna identifier (UAI) sequence that can provide spatial location of the network, as well as biomolecules by transfer of the UAI to reporter oligonucleotides associated with other antennas and biomolecules. The methods and systems are also applicable to single cells.

公开(公告)号：US11072816B2

公开(公告)日：2021-07-27

申请号：US15970791

申请日：2018-05-03

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Jellert Gaublomme ,  Aviv Regev

IPC分类号： C12Q1/6818 ,  G01N33/68 ,  C12N15/115 ,  G01N33/543

摘要： The application relates to proteome analysis in single cells. Specifically, disclosed are high throughput methods of detecting proteins in single cells using barcoding, aptamers and single cell sequencing. Solid supports used in recording the cell-of-origin of target proteins and target proteins expressed in the cell-of-origin are disclosed. Additionally, methods of detecting proteins and mRNA in single cells are disclosed. Additionally, methods of detecting protein interactions are disclosed. Additionally, methods of detecting post translationally modified proteins in single cells are disclosed. The application also relates to solid supports or beads and methods of producing said solid supports or beads for use in the described methods.