公开(公告)号：US11427869B2

公开(公告)日：2022-08-30

申请号：US15687089

申请日：2017-08-25

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  THE BRIGHAM AND WOMEN'S HOSPITAL, INC. ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Aviv Regev ,  Vijay K. Kuchroo ,  Jellert Gaublomme ,  Youjin Lee ,  Chao Wang ,  Nir Yosef ,  Hongkun Park ,  James Kaminski

IPC分类号： C12Q1/6883 ,  C12Q1/6881 ,  G01N33/50

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

公开(公告)号：US20170349950A1

公开(公告)日：2017-12-07

申请号：US15687089

申请日：2017-08-25

申请人： THE BROAD INSTITUTE INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  THE BRIGHAM AND WOMEN'S HOSPITAL, INC. ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Aviv Regev ,  Vijay K. Kuchroo ,  Jellert Gaublomme ,  Youjin Lee ,  Chao Wang ,  Nir Yosef ,  Hongkun Park ,  James Kaminski

IPC分类号： C12Q1/68 ,  G01N33/50

CPC分类号： C12Q1/6883 ,  C12Q1/6881 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/158 ,  G01N33/5023

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

公开(公告)号：US11209440B2

公开(公告)日：2021-12-28

申请号：US15245748

申请日：2016-08-24

申请人： THE BROAD INSTITUTE, INC. ,  THE BRIGHAM AND WOMEN'S HOSPITAL, INC. ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Vijay Kuchroo ,  Aviv Regev ,  Jellert Gaublomme ,  Youjin Lee ,  Alexander K. Shalek ,  Chao Wang ,  Nir Yosef ,  Hongkun Park

IPC分类号： G01N33/68 ,  C12Q1/6883 ,  A61K48/00 ,  C12N15/68 ,  C12N15/86 ,  C07K16/28

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

公开(公告)号：US10822587B2

公开(公告)日：2020-11-03

申请号：US14837702

申请日：2015-08-27

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College ,  The Brigham and Women's Hospital, Inc.

发明人： Aviv Regev ,  Vijay Kuchroo ,  Hongkun Park ,  Nir Yosef ,  Alexander K. Shalek ,  Jellert Gaublomme ,  Nicole C. Joller ,  Chuan Wu ,  Ana Carrizosa Anderson

IPC分类号： C12N5/071 ,  C12N5/00 ,  C12N5/02 ,  C07H21/04 ,  C12N5/0783 ,  C12Q1/6881 ,  A61K31/12 ,  A61K31/198 ,  A61K31/37 ,  A61K31/4745 ,  A61K31/66 ,  A61K31/661 ,  A61K31/7076 ,  A61K31/7088 ,  A61K31/739 ,  A61K33/00 ,  A61K33/06 ,  A61K33/24 ,  A61K38/17 ,  A61K38/18 ,  A61K38/19 ,  A61K38/36 ,  A61K38/48 ,  C07K16/28 ,  C12N15/113 ,  G01N33/569 ,  A61K48/00

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

公开(公告)号：US20150361396A1

公开(公告)日：2015-12-17

申请号：US14837702

申请日：2015-08-27

申请人： THE BROAD INSTITUTE INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  THE BRIGHAM AND WOMEN'S HOSPITAL, INC.

发明人： Aviv Regev ,  Vijay Kuchroo ,  Hongkun Park ,  Nir Yosef ,  Alexander K. Shalek ,  Jellert Gaublomme ,  Nicole C. Joller ,  Chuan Wu ,  Ana Carrizosa Anderson

IPC分类号： C12N5/0783 ,  A61K31/739 ,  A61K33/24 ,  A61K38/36 ,  A61K31/4745 ,  A61K31/7076 ,  A61K31/198 ,  A61K38/17 ,  A61K31/66 ,  A61K38/48 ,  A61K38/18 ,  A61K31/661 ,  A61K33/06 ,  A61K31/37 ,  A61K31/12 ,  A61K33/00 ,  C07K16/28 ,  A61K38/19 ,  C12Q1/68 ,  G01N33/569 ,  C12N15/113 ,  A61K31/7088

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

摘要翻译： 本发明一般涉及用于鉴定调节，控制或以其他方式影响T细胞平衡的调节网络的组合物和方法，例如Th17细胞分化，维持和/或功能，以及用于开发调节网络的调节网络的组合物和方法， 控制或以其他方式影响各种治疗和/或诊断适应症中的T细胞平衡。 本发明还涉及鉴定和利用调节，控制或以其他方式影响多种治疗和/或诊断适应症中的T细胞平衡的靶基因和/或靶基因产物。

公开(公告)号：US20200081017A1

公开(公告)日：2020-03-12

申请号：US16675398

申请日：2019-11-06

申请人： The Brigham and Women's Hospital, Inc. ,  The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

发明人： Vijay K. Kuchroo ,  Aviv Regev ,  Jellert Gaublomme ,  Youjin Lee ,  Alexander K. Shalek ,  Chao Wang ,  Nir Yosef ,  Hongkun Park

IPC分类号： G01N33/68 ,  C12Q1/6883 ,  C07K16/28

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

公开(公告)号：US20150368719A1

公开(公告)日：2015-12-24

申请号：US14846219

申请日：2015-09-04

申请人： THE BROAD INSTITUTE INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Aviv Regev ,  Alexander K. Shalek ,  Rahul Satija ,  Hongkun Park

IPC分类号： C12Q1/68 ,  G01N33/50

CPC分类号： C12Q1/6883 ,  C12Q2600/158 ,  G01N33/5047 ,  G01N33/6893 ,  G01N2800/24

摘要： This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences dendritic cell (DC) response(s), for example, dendritic cell maturation, dendritic cell antiviral response(s) and/or dendritic cell inflammatory response(s), as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences dendritic cell response(s) in a variety of therapeutic and/or diagnostic indications.

摘要翻译： 本发明一般涉及用于鉴定调节，控制或以其他方式影响树突细胞（DC）反应的调节网络的组合物和方法，例如树突状细胞成熟，树突细胞抗病毒应答和/或树突状细胞炎症 响应以及利用调节，控制或以其他方式影响各种治疗和/或诊断适应症中的树突状细胞反应的调节网络的组合物和方法。

公开(公告)号：US11597964B2

公开(公告)日：2023-03-07

申请号：US15453405

申请日：2017-03-08

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

发明人： Aviv Regev ,  Evan Zane Macosko ,  Steven Andrew McCarroll ,  Alexander K. Shalek ,  Anindita Basu ,  Christopher B. Ford ,  Hongkun Park ,  David A. Weitz

IPC分类号： C12Q1/6809 ,  C12Q1/6834 ,  C12Q1/6869 ,  C12N15/10 ,  G06K19/06

摘要： The present invention generally relates to a combination of molecular barcoding and emulsion-based microfluidics to isolate, lyse, barcode, and prepare nucleic acids from individual cells in a high-throughput manner.

公开(公告)号：US11566279B2

公开(公告)日：2023-01-31

申请号：US16244058

申请日：2019-01-09

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

发明人： Aviv Regev ,  Evan Zane Macosko ,  Steven Andrew McCarroll ,  Alexander K. Shalek ,  Anindita Basu ,  Christopher B. Ford ,  Hongkun Park ,  David A. Weitz

IPC分类号： C12Q1/6809 ,  C12Q1/6834 ,  C12Q1/6869 ,  C12N15/10 ,  G06K19/06

摘要： The present invention generally relates to a combination of molecular barcoding and emulsion-based microfluidics to isolate, lyse, barcode, and prepare nucleic acids from individual cells in a high-throughput manner.

公开(公告)号：US11774396B2

公开(公告)日：2023-10-03

申请号：US17940174

申请日：2022-09-08

申请人： President and Fellows of Harvard College

发明人： Hongkun Park ,  Jeffrey T. Abbott ,  Wenxuan Wu ,  Tianyang Ye ,  Han Sae Jung ,  Donhee Ham

IPC分类号： G01N27/327 ,  B01J19/08 ,  C12Q1/02 ,  G01N33/483

CPC分类号： G01N27/3277 ,  B01J19/087 ,  C12Q1/02 ,  G01N33/4836

摘要： Disclosed herein are an apparatus for electrically assessing and/or manipulating cells. One aspect is directed to electrically mapping cells on the surface of the semiconductor substrate via cross-electrode impedance measurements. Further according to some aspects, the electrode array allows for spatially addressable electrical stimulation and/or recording of electrical signals in real-time using the CMOS circuitry. Some of these aspects are directed to using an electrode array to perform cell patterning through electrochemical gas generation, and extracellular electrochemical mapping.