公开(公告)号：US07256014B2

公开(公告)日：2007-08-14

申请号：US11190386

申请日：2005-07-27

申请人： Anthony J. Kinney ,  Hao Ni ,  Pierre E. Rouviere ,  Wonchul Suh

发明人： Anthony J. Kinney ,  Hao Ni ,  Pierre E. Rouviere ,  Wonchul Suh

IPC分类号： C12P1/00 ,  C12N1/00

CPC分类号： C07K14/415 ,  C12P7/625 ,  C12P7/6409 ,  C12P7/66 ,  C12P17/06 ,  C12P23/00 ,  C12P33/00

摘要： Expression of at least one plant oleosin gene in a microbial cell engineered to produce hydrophobic/lipophilic compounds significantly increases the overall titer of the compound. The hydrophobic nature of the oleosin core is believed to increase the microbial host cell's internal storage capacity for any hydrophobic/lipophilic compound. The method is exemplified using a bacterial host cell engineered to produce significant amount of various carotenoids.

公开(公告)号：US07105296B2

公开(公告)日：2006-09-12

申请号：US10230026

申请日：2002-08-28

申请人： Michael G. Bramucci ,  Patricia C. Brzostowicz ,  Kristy N Kostichka ,  Vasantha Nagarajan ,  Pierre E. Rouviere ,  Stuart M. Thomas

发明人： Michael G. Bramucci ,  Patricia C. Brzostowicz ,  Kristy N Kostichka ,  Vasantha Nagarajan ,  Pierre E. Rouviere ,  Stuart M. Thomas

IPC分类号： C12N9/02 ,  C12N1/20 ,  C12N15/00 ,  C12Q1/00 ,  C12Q1/68 ,  C12P21/04 ,  C07H21/04

CPC分类号： C12N9/0073 ,  C12P17/08

摘要： Genes have been isolated from a variety of bacteria encoding Baeyer-Villiger monooxygenase activity. The genes and their products are useful for the conversion of ketones to the corresponding esters. A series of motifs, common to all genes, has been identified as diagnostic for genes encoding proteins of this activity.

摘要翻译： 已经从编码Baeyer-Villiger单加氧酶活性的各种细菌中分离出基因。 基因及其产物可用于将酮转化为相应的酯。 已经鉴定了所有基因共有的一系列基序作为编码该活性蛋白质的基因的诊断。