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公开(公告)号：US07105296B2
公开(公告)日：2006-09-12
申请号：US10230026
申请日：2002-08-28
申请人： Michael G. Bramucci , Patricia C. Brzostowicz , Kristy N Kostichka , Vasantha Nagarajan , Pierre E. Rouviere , Stuart M. Thomas
发明人： Michael G. Bramucci , Patricia C. Brzostowicz , Kristy N Kostichka , Vasantha Nagarajan , Pierre E. Rouviere , Stuart M. Thomas
CPC分类号： C12N9/0073 , C12P17/08
摘要： Genes have been isolated from a variety of bacteria encoding Baeyer-Villiger monooxygenase activity. The genes and their products are useful for the conversion of ketones to the corresponding esters. A series of motifs, common to all genes, has been identified as diagnostic for genes encoding proteins of this activity.
摘要翻译： 已经从编码Baeyer-Villiger单加氧酶活性的各种细菌中分离出基因。 基因及其产物可用于将酮转化为相应的酯。 已经鉴定了所有基因共有的一系列基序作为编码该活性蛋白质的基因的诊断。
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公开(公告)号：US07034140B2
公开(公告)日：2006-04-25
申请号：US10128713
申请日：2002-04-22
申请人： Michael G. Bramucci , Patricia C. Brzostowicz , Qiong Cheng , Kristy N Kostichka , Pierre E. Rouviere , Vasantha Nagarajan , Luan Tao , Stuart M. Thomas
发明人： Michael G. Bramucci , Patricia C. Brzostowicz , Qiong Cheng , Kristy N Kostichka , Pierre E. Rouviere , Vasantha Nagarajan , Luan Tao , Stuart M. Thomas
CPC分类号： C12N15/52
摘要： Genes have been isolated from Rhodococcus erythropolis AN12 strain encoding the isoprenoid biosynthetic pathway. The genes and gene products are the first isolated from a Rhodococcus strain. The genes and gene products of the present invention may be used in a variety of ways for the production of isoprenoid compounds in a variety of organisms.
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公开(公告)号：US06461840B1
公开(公告)日：2002-10-08
申请号：US09617854
申请日：2000-07-17
IPC分类号： C12P740
CPC分类号： C12P7/46
摘要： This invention relates to a biocatalytic process to produce terephthalic acid and isophthalic acid from p-xylene and m-xylene, respectively. Terephthalic acid has been prepared by oxidizing p-xylene with bacteria belonging to the genus Burkholderia. Conversion of p-xylene into terephthalic acid is accomplished by a single bacterial strain that produces all of the requisite enzymes. In addition, this invention relates to the preparation of isophthalic acid from a mixture of m- and p-xylene.
摘要翻译： 本发明涉及分别从对二甲苯和间二甲苯生产对苯二甲酸和间苯二甲酸的生物催化方法。 对苯二甲酸已经通过用属于伯克霍尔德氏菌属的细菌氧化对二甲苯来制备。 对二甲苯转化成对苯二甲酸是通过产生所有必需酶的单一细菌菌株实现的。 此外，本发明涉及从间二甲苯和对二甲苯的混合物制备间苯二甲酸。
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公开(公告)号：US06187569B1
公开(公告)日：2001-02-13
申请号：US09342579
申请日：1999-06-29
IPC分类号： C12P100
CPC分类号： C12P7/46
摘要： This invention relates to a biocatalytic process to produce terephthalic acid and isophthalic acid from p-xylene and m-xylene, respectively. Terephthalic acid has been prepared by oxidizing p-xylene with bacteria belonging to the genus Burkholderia. Conversion of p-xylene into terephthalic acid is accomplished by a single bacterial strain that produces all of the requisite enzymes. In addition, this invention relates to the preparation of isophthalic acid from a mixture of m- and p-xylene.
摘要翻译： 本发明涉及分别从对二甲苯和间二甲苯生产对苯二甲酸和间苯二甲酸的生物催化方法。 对苯二甲酸已经通过用属于伯克霍尔德氏菌属的细菌氧化对二甲苯来制备。 对二甲苯转化成对苯二甲酸是通过产生所有必需酶的单一细菌菌株实现的。 此外，本发明涉及从间二甲苯和对二甲苯的混合物制备间苯二甲酸。
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公开(公告)号：US06797500B2
公开(公告)日：2004-09-28
申请号：US10273051
申请日：2002-10-17
申请人： Mario W. Chen , Qiong Cheng , Katharine Janet Gibson , Kristy Nan-Shan Kostichka , Stuart M. Thomas , Vasantha Nagarajan
发明人： Mario W. Chen , Qiong Cheng , Katharine Janet Gibson , Kristy Nan-Shan Kostichka , Stuart M. Thomas , Vasantha Nagarajan
IPC分类号： C12N902
CPC分类号： C12N9/0073 , C12N9/0006 , C12N9/0008 , C12N15/52 , C12P7/42 , C12P7/44 , C12P7/6409 , C12P17/08
摘要： A 10 kb gene cluster has been isolated from Rhodococcus ruber SC1 comprising genes encoding enzymes useful for the synthesis of dodecanoic diacid from cyclododecanone and other cyclic intermediates. The six specific open reading frames have been identified that are associated with dodecanoic diacid biosynthesis. In addition to the expected substrates the enzymes of the instant invention have moderate specificity for C11-C15 compounds.
摘要翻译： 已经从Rhodococcus ruber SC1中分离出10kb的基因簇，其包含编码可用于从环十二烷酮和其它环状中间体合成十二烷二酸的酶的基因。 已经鉴定了与十二烷二酸生物合成相关的六个特定的开放阅读框。 除了预期的底物外，本发明的酶对C11-C15化合物具有适度的特异性。
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6.发明授权Method for the use of polyhydroxyalkanoate levels to maintain bioreactor health 失效使用聚羟基链烷酸酯水平维持生物反应器健康的方法公开(公告)号：US06991931B2
公开(公告)日：2006-01-31
申请号：US10761590
申请日：2004-01-21
IPC分类号： B09B3/00
CPC分类号： G01N33/1866 , C02F3/006 , C12Q1/045 , G01N33/64 , G01N33/66 , G01N2333/195 , G01N2333/21 , G01N2333/22 , G01N2333/225 , G01N2333/355 , G01N2800/52
摘要： A method has been developed to maintain the health of an activated sludge environment in a wastewater process comprising monitoring the levels of polyhydroxyalkanoates (PHA) produced as an indicator of bioreactor health and regulating the concentration of feed nutrients and nitrate to maintain bioreactor health. In general, levels of PHA in excess of about 15% to about 20% dry weight of the biomass is an indication that the biocatalytic efficiency of the wastewater treatment process is impaired.
摘要翻译： 已经开发了一种维持废水处理过程中活性污泥环境的健康的方法，包括监测生成的聚羟基链烷酸酯（PHA）的水平，作为生物反应器健康的指标，并调节饲料营养物和硝酸盐的浓度以维持生物反应器的健康。 通常，超过生物质干重的约15％至约20％的PHA水平表明废水处理过程的生物催化效率受损。
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7.发明授权公开(公告)号：US07618672B2
公开(公告)日：2009-11-17
申请号：US10983316
申请日：2004-11-05
CPC分类号： C13K11/00 , A23L5/273 , A23L11/34 , A23L33/105 , A23L33/20 , A61K31/045 , C13B20/123 , C13B20/165 , Y10S423/21
摘要： The present invention relates to a process for selectively removing undesired oligosaccharides from aqueous mixtures such as plant processing waste products, including soy whey and other vegetable wheys, using an ultrastabilized large pore, hydrophobic zeolite Y. The resulting solution, which contains isoflavones and digestible sugars such as glucose, fructose and sucrose, can serve as the basis for a nutritious new product.
摘要翻译： 本发明涉及使用超稳定大孔，疏水性沸石Y从含水混合物如植物加工废物（包括大豆乳清和其它植物乳清）中选择性除去不需要的寡糖的方法。所得的溶液含有异黄酮和可消化的糖 如葡萄糖，果糖和蔗糖，可作为营养新产品的基础。
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公开(公告)号：US06794165B2
公开(公告)日：2004-09-21
申请号：US10272419
申请日：2002-10-16
IPC分类号： C12P744
摘要： A gene cluster has been isolated from an Acinetobacter sp. that encodes the enzymes expected to convert cyclohexanol to adipic acid. The entire gene cluster has been cloned and all open reading frames have been sequenced. Cosmid clones have been identified containing the gene cluster. Demonstration of conversion of cyclohexanol to adipic acid has been made with the recombinant E. coli host strain containing the cosmids.
摘要翻译： 已经从不动杆菌属中分离出一个基因簇。 其编码预期将环己醇转化为己二酸的酶。 已经克隆了整个基因簇，并且所有的开放阅读框已被测序。 确定了含有基因簇的粘粒克隆。 用包含粘粒的重组大肠杆菌宿主菌已经证实了环己醇转化为己二酸。
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公开(公告)号：US06498242B1
公开(公告)日：2002-12-24
申请号：US09648004
申请日：1999-07-14
IPC分类号： C07H2104
摘要： A gene cluster has been isolated from an Acinetobacter sp. that encodes the enzymes expected to convert cyclohexanol to adipic acid. The entire gene cluster has been cloned and all open reading frames have been sequenced. Cosmid clones have been identified containing the gene cluster. Demonstration of conversion of cyclohexanol to adipic acid has been made with the recombinant E. coli host strain containing the cosmids.
摘要翻译： 已经从不动杆菌属中分离出一个基因簇。 其编码预期将环己醇转化为己二酸的酶。 已经克隆了整个基因簇，并且所有的开放阅读框已被测序。 确定了含有基因簇的粘粒克隆。 用包含粘粒的重组大肠杆菌宿主菌已经证实了环己醇转化为己二酸。
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10.发明授权Recovery of isoflavones and removal of oligosaccharides from aqueous mixtures using zeolites 失效异构体的回收和使用沸石从含水混合物中除去寡糖公开(公告)号：US07618671B2
公开(公告)日：2009-11-17
申请号：US10983125
申请日：2004-11-05
CPC分类号： A23L33/185 , A23L5/273 , Y10S423/21 , Y10S423/27
摘要： The present invention provides a process using zeolites for recovering isoflavones and removing undesired oligosaccharides from aqueous mixtures, such as soy whey and other plant processing waste products. The aqueous mixture is treated with a large pore hydrophobic zeolite, such as zeolite Beta, to remove isoflavones, followed by treatment with an ultrastabilized, hydrophobic zeolite Y, such as CBV-901 or HiSiv™ 4000, to remove the undesired oligosaccharides raffinose and stachyose. The recovered isoflavones and digestible sugars, glucose, sucrose, and fructose, are useful in food products.
摘要翻译： 本发明提供了一种使用沸石回收异黄酮并从含水混合物如大豆乳清和其它植物加工废物中除去不期望的寡糖的方法。 用大孔疏水性沸石如β沸石处理含水混合物以除去异黄酮，然后用超稳定的疏水性沸石Y（例如CBV-901或HiSiv TM 4000）处理以除去不期望的寡糖棉子糖 和水苏糖 回收的异黄酮和可消化糖，葡萄糖，蔗糖和果糖，可用于食品。
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