公开(公告)号：US11454758B2

公开(公告)日：2022-09-27

申请号：US16987937

申请日：2020-08-07

Applicant: Quantum-Si Incorporated

Inventor： Ali Kabiri ,  Bing Shen ,  Gerard Schmid ,  James Beach ,  Kyle Preston ,  Sharath Hosali

IPC: G02B6/122 ,  G02B6/136 ,  G02B6/12

Abstract: Apparatus and methods for improving optical signal collection in an integrated device are described. A microdisk can be formed in an integrated device and increase collection and/or concentration of radiation incident on the microdisk and re-radiated by the microdisk. An example integrated device that can include a microdisk may be used for analyte detection and/or analysis. Such an integrated device may include a plurality of pixels, each having a reaction chamber for receiving a sample to be analyzed, an optical microdisk, and an optical sensor configured to detect optical emission from the reaction chamber. The microdisk can comprise a dielectric material having a first index of refraction that is embedded in one or more surrounding materials having one or more different refractive index values.

公开(公告)号：US11422092B2

公开(公告)日：2022-08-23

申请号：US17161425

申请日：2021-01-28

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Gerard Schmid ,  Keith G. Fife ,  James Beach ,  Jason W. Sickler ,  Lawrence C. West ,  Paul E. Glenn ,  Kyle Preston ,  Farshid Ghasemi ,  Benjamin Cipriany ,  Jeremy Lackey

IPC: G01N21/64 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/77 ,  G01N33/543

Abstract: System and methods for analyzing single molecules and performing nucleic acid sequencing. An integrated device may include multiple pixels with sample wells configured to receive a sample, which when excited, emits radiation. The integrated device includes a surface having a trench region recessed from a portion of the surface and an array of sample wells, disposed in the trench region. The integrated device also includes a waveguide configured to couple excitation energy to at least one sample well in the array and positioned at a first distance from a surface of the trench region and at a second distance from the surface in a region separate from the trench region. The first distance is smaller than the second distance. The system also includes an instrument that interfaces with the integrated device. The instrument may include an excitation energy source for providing excitation energy to the integrated device by coupling to an excitation energy coupling region of the integrated device.

公开(公告)号：US20220128566A1

公开(公告)日：2022-04-28

申请号：US17511258

申请日：2021-10-26

Applicant: Quantum-Si Incorporated

Inventor： Thomas Christian ,  Kyle Preston ,  Brian Reed ,  Shannon Stewman

IPC: G01N33/58 ,  G01N21/64

Abstract: Aspects of the present disclosure relate to techniques for calibrating an integrated device. According to some embodiments, there is provided a method for calibrating an integrated device, the method comprising: exciting, with light from at least one excitation source, a reference dye molecule; obtaining a signal emitted by the reference dye molecule, the signal containing information representative of at least one characteristic of the reference dye molecule; and adjusting one or more subsequent measurements obtained from a sample based on the information obtained from the signal emitted by the reference dye molecule.

公开(公告)号：US20220113469A1

公开(公告)日：2022-04-14

申请号：US17555323

申请日：2021-12-17

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Gerard Schmid ,  Alexander Gondarenko ,  James Beach ,  Kyle Preston ,  Farshid Ghasemi ,  Jeremy Lackey ,  Jack Jewell ,  Keith G. Fife ,  Ali Kabiri

IPC: G02B6/122 ,  G02B26/08 ,  G02B5/30 ,  G02B6/02 ,  G02B5/20 ,  H01L25/16 ,  G01N21/64

Abstract: An integrated device and related instruments and systems for analyzing samples in parallel are described. The integrated device may include sample wells arranged on a surface of where individual sample wells are configured to receive a sample labeled with at least one fluorescent marker configured to emit emission light in response to excitation light. The integrated device may further include photodetectors positioned in a layer of the integrated device, where one or more photodetectors are positioned to receive a photon of emission light emitted from a sample well. The integrated device further includes one or more photonic structures positioned between the sample wells and the photodetectors, where the one or more photonic structures are configured to attenuate the excitation light relative to the emission light such that a signal generated by the one or more photodetectors indicates detection of photons of emission light.

公开(公告)号：US11226290B2

公开(公告)日：2022-01-18

申请号：US15611583

申请日：2017-06-01

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Gerard Schmid ,  Keith G. Fife ,  James Beach ,  Jason W. Sickler ,  Lawrence C. West ,  Paul E. Glenn ,  Kyle Preston ,  Farshid Ghasemi ,  Benjamin Cipriany ,  Jeremy Lackey

IPC: G01N21/64 ,  C12Q1/68 ,  G01N21/77 ,  G01N33/543 ,  C12Q1/6869 ,  C12Q1/6874

Abstract: System and methods for analyzing single molecules and performing nucleic acid sequencing. An integrated device may include multiple pixels with sample wells configured to receive a sample, which when excited, emits radiation. The integrated device includes a surface having a trench region recessed from a portion of the surface and an array of sample wells, disposed in the trench region. The integrated device also includes a waveguide configured to couple excitation energy to at least one sample well in the array and positioned at a first distance from a surface of the trench region and at a second distance from the surface in a region separate from the trench region. The first distance is smaller than the second distance. The system also includes an instrument that interfaces with the integrated device. The instrument may include an excitation energy source for providing excitation energy to the integrated device by coupling to an excitation energy coupling region of the integrated device.

公开(公告)号：US20210215606A1

公开(公告)日：2021-07-15

申请号：US17149605

申请日：2021-01-14

Applicant: Quantum-Si Incorporated

Inventor： Gerard Schmid ,  Sharath Hosali ,  James Beach ,  Kyle Preston ,  Ali Kabiri ,  Bing Shen

IPC: G01N21/64 ,  G02B6/14 ,  G02B6/122 ,  B01L3/00 ,  C12Q1/6816

Abstract: Systems and methods for optical power distribution within an integrated device, in a substantially uniform manner, to a large number of sample wells and/or other photonic elements. The integrated device and related instruments and systems may be used to analyze samples in parallel. The integrated device may include a grating coupler configured to receive light from an excitation source and optically couple with multiple waveguides configured to couple with sample wells. Vertical extents of optical modes of individual waveguides may be modulated to adjust confinement of light within the waveguides. This modulation may enable more uniform distribution of excitation light to the sample wells, improve excitation efficiency, and prevent overpower on regions of the integrated device.