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公开(公告)号：US20240248034A1
公开(公告)日：2024-07-25
申请号：US18518036
申请日：2023-11-22
发明人： Jonathan M. Rothberg , Ali Kabiri , Jason W. Sickler , Brett J. Gyarfas , Jeremy Lackey , Gerard Schmid , Paul E. Glenn , Lawrence C. West , Benjamin Cipriany , Keith G. Fife
IPC分类号： G01N21/64 , C12Q1/6874
CPC分类号： G01N21/6408 , C12Q1/6874 , G01N21/6428 , G01N21/6452 , G01N21/648 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2201/0446 , G01N2201/062
摘要： Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.
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公开(公告)号：US11977257B2
公开(公告)日：2024-05-07
申请号：US17893101
申请日：2022-08-22
发明人： Ali Kabiri , Bing Shen , Gerard Schmid , James Beach , Kyle Preston , Sharath Hosali
CPC分类号： G02B6/122 , G02B6/136 , G02B2006/12109 , G02B2006/12138
摘要： Apparatus and methods for improving optical signal collection in an integrated device are described. A microdisk can be formed in an integrated device and increase collection and/or concentration of radiation incident on the microdisk and re-radiated by the microdisk. An example integrated device that can include a microdisk may be used for analyte detection and/or analysis. Such an integrated device may include a plurality of pixels, each having a reaction chamber for receiving a sample to be analyzed, an optical microdisk, and an optical sensor configured to detect optical emission from the reaction chamber. The microdisk can comprise a dielectric material having a first index of refraction that is embedded in one or more surrounding materials having one or more different refractive index values.
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公开(公告)号：US11932906B2
公开(公告)日：2024-03-19
申请号：US17350509
申请日：2021-06-17
发明人： Guojun Chen , Jeremy Lackey , Alexander Goryaynov , Gerard Schmid , Ali Kabiri , Jonathan M. Rothberg , Todd Rearick , Jonathan C. Schultz , Farshid Ghasemi , Keith G. Fife
IPC分类号： C12Q1/6869 , B01L3/00 , C08L1/02 , C12Q1/6806 , G01N21/64 , G01N27/447 , G01N30/86 , G01N30/88
CPC分类号： C12Q1/6869 , B01L3/502761 , B01L3/50857 , C08L1/02 , C12Q1/6806 , G01N21/6428 , G01N21/6454 , G01N27/44743 , G01N27/44782 , G01N30/86 , B01L2200/0647 , B01L2300/0645 , B01L2300/0829 , B01L2300/0864 , B01L2400/0415 , B01L2400/0421 , G01N27/447 , G01N30/88
摘要： Apparatus and techniques for electrokinetic loading of samples of interest into sub-micron-scale reaction chambers are described. Embodiments include an integrated device and related apparatus for analyzing samples in parallel. The integrated device may include at least one reaction chamber formed through a surface of the integrated device and configured to receive a sample of interest, such as a molecule of nucleic acid. The integrated device may further include electrodes patterned adjacent to the reaction chamber that produce one or more electric fields that assist loading the sample into the reaction chamber. The apparatus may further include a sample reservoir having a fluid seal with the surface of the integrated device and configured to hold a suspension containing the samples.
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公开(公告)号：US11879841B2
公开(公告)日：2024-01-23
申请号：US17497827
申请日：2021-10-08
发明人： Jonathan M. Rothberg , Ali Kabiri , Jason W. Sickler , Brett J. Gyarfas , Jeremy Lackey , Gerard Schmid , Paul E. Glenn , Lawrence C. West , Benjamin Cipriany , Keith G. Fife
IPC分类号： G01N21/64 , C12Q1/6874
CPC分类号： G01N21/6408 , C12Q1/6874 , G01N21/648 , G01N21/6428 , G01N21/6452 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2201/0446 , G01N2201/062
摘要： Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.
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公开(公告)号：US20230358958A1
公开(公告)日：2023-11-09
申请号：US18355704
申请日：2023-07-20
发明人： Gerard Schmid , Kyle Preston , Shannon Stewman
CPC分类号： G02B6/124 , G01N21/6428 , G02B6/12004 , G01N2021/6439 , G02B2006/12107
摘要： Apparatus and methods relating to coupling radiation from an incident beam into a plurality of waveguides with a grating coupler are described. A grating coupler can have offset receiving regions and grating portions with offset periodicity to improve sensitivity of the grating coupler to misalignment of the incident beam.
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公开(公告)号：US11719639B2
公开(公告)日：2023-08-08
申请号：US17190331
申请日：2021-03-02
发明人： Gerard Schmid , Dajiang Yang , Eric A. G. Webster , Xin Wang , Todd Rearick , Changhoon Choi , Ali Kabiri , Kyle Preston , Brian Reed
IPC分类号： G01N21/64 , H01L27/146
CPC分类号： G01N21/6454 , G01N21/6408 , G01N21/6428 , H01L27/14603 , G01N2021/6463
摘要： Some aspects relate to an integrated circuit, comprising at least one photodetection region configured to generate charge carriers responsive to incident photons emitted from a sample, at least one charge storage region configured to receive the charge carriers from the photodetection region, and at least one controller configured to obtain information about the incident photons, the information comprising at least one member selected from the group comprising pulse duration and interpulse duration and at least one member selected from the group comprising wavelength information, luminescence lifetime information, and intensity information. In some embodiments, the information comprises at least three, four, and/or five members selected from the group comprising wavelength information, luminescence lifetime information, intensity information, pulse duration information, and interpulse duration information. In some embodiments, the information obtained may be used to identify the sample.
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公开(公告)号：US20230221253A1
公开(公告)日：2023-07-13
申请号：US18095767
申请日：2023-01-11
发明人： Brian Reed , Todd Rearick , Gerard Schmid , Haidong Huang
CPC分类号： G01N21/6428 , G01N33/6821 , G01N33/6824 , G01N33/582 , G01N1/28 , G01N2021/6439 , G01N2458/00 , G16B30/00
摘要： Techniques for multi-dimensional signal analysis are described herein. The techniques may be used in one or more sequencing applications. For example, according to some aspects, there is provided a method comprising: determining information about a sample that emits emission light in response to excitation light based on at least one of pulse duration and interpulse duration and at least two of wavelength, intensity, and lifetime of the emission light, wherein the sample comprises a reagent configured to be coupled to a luminescent label, and wherein a shielding element is disposed between the reagent and the luminescent label.
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8.发明授权公开(公告)号：US10712274B2
公开(公告)日：2020-07-14
申请号：US15974826
申请日：2018-05-09
发明人： Jonathan M. Rothberg , Ali Kabiri , Jason W. Sickler , Brett J. Gyarfas , Jeremy Lackey , Gerard Schmid
IPC分类号： G01N21/00 , G01N21/64 , C12Q1/6874 , G01N21/77 , B01L3/00 , C12Q1/6869
摘要： An active-source-pixel, integrated device capable of performing biomolecule detection and/or analysis, such as single-molecule nucleic acid sequencing, is described. An active pixel of the integrated device includes a sample well into which a sample to be analyzed may diffuse, an excitation source for providing excitation energy to the sample well, and a sensor configured to detect emission from the sample. The sensor may comprise two or more segments that produce a set of signals that are analyzed to differentiate between and identify tags that are attached to, or associated with, the sample. Tag differentiation may be spectral and/or temporal based. Identification of the tags may be used to detect, analyze, and/or sequence the biomolecule.
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公开(公告)号：US20200171484A1
公开(公告)日：2020-06-04
申请号：US16580877
申请日：2019-09-24
发明人： Guojun Chen , James Beach , Kathren Fink , Jeremy Lackey , Gerard Schmid
摘要： A method of forming an integrated device includes forming a sample well within a cladding layer of a substrate; forming a sacrificial spacer layer over the substrate and into the sample well; performing a directional etch of the sacrificial spacer layer so as to form a sacrificial sidewall spacer on sidewalls of the sample well; forming, over the substrate and into the sample well, a functional layer that provides a location for attachment of a biomolecule; and removing the sacrificial spacer material.
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公开(公告)号：US10533945B2
公开(公告)日：2020-01-14
申请号：US15808940
申请日：2017-11-10
发明人： Jonathan M. Rothberg , Ali Kabiri , Jason W. Sickler , Brett J. Gyarfas , Jeremy Lackey , Gerard Schmid , Benjamin Cipriany , Jack Jewell , Lawrence C. West , Michael Ferrigno , Paul E. Glenn , Adam Ezra Cohen , Anthony Bellofiore
IPC分类号： G01N21/64 , C12Q1/6874 , G01N21/77 , C12Q1/6869 , B01L3/00
摘要： Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.
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