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公开(公告)号：US20240272169A1
公开(公告)日：2024-08-15
申请号：US18598736
申请日：2024-03-07
发明人： Brian Reed , Jeremy Lackey , Haidong Huang
IPC分类号： G01N33/58 , C07K14/47 , C07K19/00 , C12Q1/6806 , G01N1/28 , G01N21/64 , G01N33/68 , G16B25/10 , G16B30/00 , G16B40/00 , G16B40/10 , G16B50/00 , G16B50/30
CPC分类号： G01N33/581 , C07K14/47 , C07K19/00 , C12Q1/6806 , G01N1/28 , G01N21/6428 , G01N33/58 , G01N33/582 , G01N33/6821 , G01N33/6824 , G16B25/10 , G16B40/00 , G16B40/10 , G16B50/30 , G01N2021/6439 , G01N2458/00 , G16B30/00 , G16B50/00
摘要： Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.
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公开(公告)号：US12055548B2
公开(公告)日：2024-08-06
申请号：US16709077
申请日：2019-12-10
发明人： Brian Reed , Jeremy Lackey , Haidong Huang
IPC分类号： A61K38/00 , C07K14/47 , C07K19/00 , C12Q1/6806 , G01N1/28 , G01N21/64 , G01N33/58 , G01N33/68 , G16B25/10 , G16B40/00 , G16B40/10 , G16B50/30 , G16B30/00 , G16B50/00
CPC分类号： G01N33/581 , C07K14/47 , C07K19/00 , C12Q1/6806 , G01N1/28 , G01N21/6428 , G01N33/58 , G01N33/582 , G01N33/6821 , G01N33/6824 , G16B25/10 , G16B40/00 , G16B40/10 , G16B50/30 , G01N2021/6439 , G01N2458/00 , G16B30/00 , G16B50/00
摘要： Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.
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公开(公告)号：US20240248034A1
公开(公告)日：2024-07-25
申请号：US18518036
申请日：2023-11-22
发明人： Jonathan M. Rothberg , Ali Kabiri , Jason W. Sickler , Brett J. Gyarfas , Jeremy Lackey , Gerard Schmid , Paul E. Glenn , Lawrence C. West , Benjamin Cipriany , Keith G. Fife
IPC分类号： G01N21/64 , C12Q1/6874
CPC分类号： G01N21/6408 , C12Q1/6874 , G01N21/6428 , G01N21/6452 , G01N21/648 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2201/0446 , G01N2201/062
摘要： Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.
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公开(公告)号：US11932906B2
公开(公告)日：2024-03-19
申请号：US17350509
申请日：2021-06-17
发明人： Guojun Chen , Jeremy Lackey , Alexander Goryaynov , Gerard Schmid , Ali Kabiri , Jonathan M. Rothberg , Todd Rearick , Jonathan C. Schultz , Farshid Ghasemi , Keith G. Fife
IPC分类号： C12Q1/6869 , B01L3/00 , C08L1/02 , C12Q1/6806 , G01N21/64 , G01N27/447 , G01N30/86 , G01N30/88
CPC分类号： C12Q1/6869 , B01L3/502761 , B01L3/50857 , C08L1/02 , C12Q1/6806 , G01N21/6428 , G01N21/6454 , G01N27/44743 , G01N27/44782 , G01N30/86 , B01L2200/0647 , B01L2300/0645 , B01L2300/0829 , B01L2300/0864 , B01L2400/0415 , B01L2400/0421 , G01N27/447 , G01N30/88
摘要： Apparatus and techniques for electrokinetic loading of samples of interest into sub-micron-scale reaction chambers are described. Embodiments include an integrated device and related apparatus for analyzing samples in parallel. The integrated device may include at least one reaction chamber formed through a surface of the integrated device and configured to receive a sample of interest, such as a molecule of nucleic acid. The integrated device may further include electrodes patterned adjacent to the reaction chamber that produce one or more electric fields that assist loading the sample into the reaction chamber. The apparatus may further include a sample reservoir having a fluid seal with the surface of the integrated device and configured to hold a suspension containing the samples.
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公开(公告)号：US11879841B2
公开(公告)日：2024-01-23
申请号：US17497827
申请日：2021-10-08
发明人： Jonathan M. Rothberg , Ali Kabiri , Jason W. Sickler , Brett J. Gyarfas , Jeremy Lackey , Gerard Schmid , Paul E. Glenn , Lawrence C. West , Benjamin Cipriany , Keith G. Fife
IPC分类号： G01N21/64 , C12Q1/6874
CPC分类号： G01N21/6408 , C12Q1/6874 , G01N21/648 , G01N21/6428 , G01N21/6452 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2201/0446 , G01N2201/062
摘要： Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.
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6.发明申请公开(公告)号：US20220305487A1
公开(公告)日：2022-09-29
申请号：US17545127
申请日：2021-12-08
发明人： Jonathan M. Rothberg , Jeremy Lackey , Guojun Chen
IPC分类号： B01L3/00 , C12Q1/6848 , C09D5/16 , C09D187/00 , C09D171/02
摘要： Methods of preparing surfaces of sample wells are provided. In some aspects, methods of preparing a sample well surface involve contacting the sample well with a block copolymer to form an antifouling overlay over a metal oxide surface of the sample well. In some aspects, methods of passivating and/or selectively functionalizing a sample well surface are provided.
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公开(公告)号：US20220073973A1
公开(公告)日：2022-03-10
申请号：US17314626
申请日：2021-05-07
发明人： Jonathan M. Rothberg , Jeremy Lackey , Brian Reed , Xinghua Shi , Haidong Huang
IPC分类号： C12Q1/6818 , C12Q1/6869 , G01N21/64 , C12Q1/6874
摘要： Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction.
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8.发明授权公开(公告)号：US10712274B2
公开(公告)日：2020-07-14
申请号：US15974826
申请日：2018-05-09
发明人： Jonathan M. Rothberg , Ali Kabiri , Jason W. Sickler , Brett J. Gyarfas , Jeremy Lackey , Gerard Schmid
IPC分类号： G01N21/00 , G01N21/64 , C12Q1/6874 , G01N21/77 , B01L3/00 , C12Q1/6869
摘要： An active-source-pixel, integrated device capable of performing biomolecule detection and/or analysis, such as single-molecule nucleic acid sequencing, is described. An active pixel of the integrated device includes a sample well into which a sample to be analyzed may diffuse, an excitation source for providing excitation energy to the sample well, and a sensor configured to detect emission from the sample. The sensor may comprise two or more segments that produce a set of signals that are analyzed to differentiate between and identify tags that are attached to, or associated with, the sample. Tag differentiation may be spectral and/or temporal based. Identification of the tags may be used to detect, analyze, and/or sequence the biomolecule.
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公开(公告)号：US20200219590A1
公开(公告)日：2020-07-09
申请号：US16686028
申请日：2019-11-15
发明人： Brian Reed , Jeremy Lackey , Thomas Christian , Roger Nani , David Dodd , Kathren Croce , Alexander Goryaynov , Jonathan M. Rothberg
IPC分类号： G16B50/30 , G16B25/10 , G16B40/00 , C12Q1/6806
摘要： Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.
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公开(公告)号：US20200209253A1
公开(公告)日：2020-07-02
申请号：US16708956
申请日：2019-12-10
发明人： Brian Reed , Jeremy Lackey , Thomas Christian , Roger Nani , David Dodd , Kathren Croce , Alexander Goryaynov , Jonathan M. Rothberg
摘要： Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.
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