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91 条记录 (耗时 0.052 秒)

    Beta-lactamase CD4+ T-cell epitopes
    41.
    发明申请
    Beta-lactamase CD4+ T-cell epitopes 审中-公开
    β-内酰胺酶CD4 + T细胞表位

    公开(公告)号：US20050164308A1

    公开(公告)日：2005-07-28

    申请号：US11011422

    申请日：2004-12-14

    申请人： Fiona Harding ,  Volker Schellenberger

    发明人： Fiona Harding ,  Volker Schellenberger

    IPC分类号： C12N9/86 ,  G01N33/53 ,  A61K39/02 ,  C12N5/08 ,  C12N9/80 ,  G01N33/567

    CPC分类号： C12N9/86

    摘要： The present invention provides beta-lactamase CD4+ T-cell epitopes, as well as novel variants that exhibit reduced immunogenic responses, as compared to the parental beta-lactamase. The present invention further provides DNA molecules that encode novel beta-lactamase variants, host cells comprising DNA encoding novel beta-lactamase variants, as well as methods for making beta-lactamases less immunogenic. In addition, the present invention provides various compositions that comprise these beta-lactamase variants that are less immunogenic than wild-type beta-lactamases.

    摘要翻译： 与亲本β-内酰胺酶相比，本发明提供了β-内酰胺酶CD4 + T细胞表位，以及表现出降低的免疫原性应答的新型变体。 本发明进一步提供了编码新型β-内酰胺酶变体的DNA分子，包含编码新型β-内酰胺酶变体的DNA的宿主细胞，以及制备较少免疫原性的β-内酰胺酶的方法。 此外，本发明提供了包含与野生型β-内酰胺酶相比免疫原性较低的这些β-内酰胺酶变体的各种组合物。

    Directed evolution of microorganisms
    45.
    发明授权
    Directed evolution of microorganisms 失效
    微生物的定向进化

    公开(公告)号：US06706503B2

    公开(公告)日：2004-03-16

    申请号：US10037677

    申请日：2001-10-23

    申请人： Volker Schellenberger ,  Amy D. Liu ,  Olga V. Selifonova

    发明人： Volker Schellenberger ,  Amy D. Liu ,  Olga V. Selifonova

    IPC分类号： C12P104

    CPC分类号： C12N9/0006 ,  C07K14/245 ,  C12N15/102 ,  C12P7/18 ,  C12R1/01 ,  C12R1/19

    摘要： The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention and provides ATCC deposits which exemplify the evolved microorganisms produced by the methods.

    摘要翻译： 本发明提供了用于引导微生物进化的方法，所述微生物包括使用突变基因并在选择压力条件下生长。 该方法公开了可用于本发明方法的突变基因，并提供了例证通过该方法产生的进化微生物的ATCC沉积物。

    Multiply-substituted protease variants with altered net charge for use in detergents
    46.
    发明授权
    Multiply-substituted protease variants with altered net charge for use in detergents 有权

    公开(公告)号：US06673590B1

    公开(公告)日：2004-01-06

    申请号：US09177353

    申请日：1998-10-23

    申请人： Ayrookaran .J. Poulose ,  Volker Schellenberger ,  James T. Kellis, Jr. ,  Christian Paech ,  Joanne Nadherny ,  Donald P. Naki ,  Katherine D. Collier ,  Robert M. Caldwell

    发明人： Ayrookaran .J. Poulose ,  Volker Schellenberger ,  James T. Kellis, Jr. ,  Christian Paech ,  Joanne Nadherny ,  Donald P. Naki ,  Katherine D. Collier ,  Robert M. Caldwell

    IPC分类号： C12N954

    CPC分类号： C12N9/54 ,  A23G4/123 ,  A23K20/189 ,  A61K8/66 ,  A61K2800/86 ,  A61Q11/00 ,  A61Q19/00 ,  C11D3/386 ,  C11D3/38609 ,  C11D3/38618 ,  C11D3/3907 ,  C11D3/3945 ,  C12Y304/00 ,  C12Y304/21062

    摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a low detergent concentration system than a precursor protease. Also provided are protease variants containing substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and thus the protease variant is more effective in a high detergent concentration system than a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a medium detergent concentration system than a precursor protease. Also provided are protease variants containing substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and thus the protease variant is more effective in a medium detergent concentration system than a precursor protease. Further provided is a method of producing a protease variant that is more effective in a low detergent concentration system, medium detergent concentration system and high detergent concentration system than a precursor protease.

    Directed evolution of microorganisms
    48.
    发明授权
    Directed evolution of microorganisms 有权
    微生物的定向进化

    公开(公告)号：US06365410B1

    公开(公告)日：2002-04-02

    申请号：US09314847

    申请日：1999-05-19

    申请人： Volker Schellenberger ,  Amy D. Liu ,  Olga V. Selifonova

    发明人： Volker Schellenberger ,  Amy D. Liu ,  Olga V. Selifonova

    IPC分类号： C12N1574

    CPC分类号： C12N9/0006 ,  C07K14/245 ,  C12N15/102 ,  C12P7/18 ,  C12R1/01 ,  C12R1/19

    摘要： The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention and provides ATCC deposits which exemplify the evolved microorganisms produced by the methods.

    摘要翻译： 本发明提供了用于引导微生物进化的方法，所述微生物包括使用突变基因并在选择压力条件下生长。 该方法公开了可用于本发明方法的突变基因，并提供了例证通过该方法产生的进化微生物的ATCC沉积物。