利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

104 条记录 (耗时 0.049 秒)

    Methods for generating genetically altered antibody producing cell lines with improved antibody characteristics
    42.
    发明授权
    Methods for generating genetically altered antibody producing cell lines with improved antibody characteristics 有权
    用于产生具有改善的抗体特征的遗传改变的产生抗体的细胞系的方法

    公开(公告)号：US06808894B1

    公开(公告)日：2004-10-26

    申请号：US09707468

    申请日：2000-11-07

    申请人： Nicholas C. Nicolaides ,  Luigi Grasso ,  Philip M. Sass

    发明人： Nicholas C. Nicolaides ,  Luigi Grasso ,  Philip M. Sass

    IPC分类号： C12N1500

    CPC分类号： C07K16/00 ,  C07K2317/567

    摘要： Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into cells and transgenic animals, new cell lines and animal varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation. These methods are useful for generating genetic diversity within immunoglobulin genes directed against an antigen of interest to produce altered antibodies with enhanced biochemical activity. Moreover, these methods are useful for generating antibody-producing cells with increased level of antibody production.

    摘要翻译： 人类错配修复基因的主要阴性等位基因可用于产生超可变细胞和生物体。 通过将这些基因引入细胞和转基因动物，可以比通过依赖于突变的自然速率更有效地制备具有新颖和有用性质的新细胞系和动物品种。 这些方法可用于在针对感兴趣的抗原的免疫球蛋白基因内产生遗传多样性，以产生具有增强的生物化学活性的改变的抗体。 此外，这些方法可用于产生具有增加的抗体产生水平的产生抗体的细胞。

    Method for generating genetically altered antigens
    43.
    发明授权
    Method for generating genetically altered antigens 有权
    产生遗传改变抗原的方法

    公开(公告)号：US06737268B1

    公开(公告)日：2004-05-18

    申请号：US09712691

    申请日：2000-11-14

    申请人： Nicholas C. Nicolaides ,  Luigi Grasso ,  Philip M. Sass

    发明人： Nicholas C. Nicolaides ,  Luigi Grasso ,  Philip M. Sass

    IPC分类号： C12N1500

    CPC分类号： C12N15/1024

    摘要： Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into cells and transgenic animals, new cell lines and animal varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation. These methods are useful for generating genetic diversity within genes encoding for therapeutic antigens to produce altered polypeptides with enhanced antigenic and immunogenic activity. Moreover, these methods are useful for generating effective vaccines.

    摘要翻译： 人类错配修复基因的主要阴性等位基因可用于产生超可变细胞和生物体。 通过将这些基因引入细胞和转基因动物，可以比通过依赖于突变的自然速率更有效地制备具有新颖和有用性质的新细胞系和动物品种。 这些方法可用于在编码治疗性抗原的基因内产生遗传多样性，以产生具有增强的抗原性和免疫原性活性的改变的多肽。 此外，这些方法可用于产生有效的疫苗。

    Methods Of Generating High-Production Of Antibodies From Hybridomas Created By In Vitro Immunization
    49.
    发明申请
    Methods Of Generating High-Production Of Antibodies From Hybridomas Created By In Vitro Immunization 有权
    通过体外免疫产生的杂交瘤产生高产抗体的方法

    公开(公告)号：US20100311169A1

    公开(公告)日：2010-12-09

    申请号：US12786530

    申请日：2010-05-25

    申请人： Luigi Grasso ,  Shaohong Liang ,  Nicholas C. Nicolaides ,  Philip M. Sass

    发明人： Luigi Grasso ,  Shaohong Liang ,  Nicholas C. Nicolaides ,  Philip M. Sass

    IPC分类号： C12N15/06 ,  C12N15/01

    CPC分类号： C12N15/01 ,  C07K16/00 ,  C07K16/1282 ,  C07K16/243 ,  C07K16/2863 ,  C07K16/30 ,  C07K2317/21 ,  C07K2317/73 ,  C12N15/1024

    摘要： The invention provides methods for generating high titers of high-affinity antibodies from hybridoma cells produced by fusing myeloma cells with in vitro immunized donor cells. The hybridoma cells or mammalian expression cells with cloned antibody genes from the hybridomas producing the high-affinity antibodies may be mismatch repair defective due to defects of endogenous mismatch repair subunits of through expression of a dominant negative allele of a mismatch repair gene which allows the hybridoma cell to be hypermutable, may be rendered hypermutable by chemical means, or may be naturally mismatch repair deficient. High-affinity antibodies and high titer producer cells producing antibodies may be prepared by the methods of the invention.

    摘要翻译： 本发明提供了通过将骨髓瘤细胞与体外免疫的供体细胞融合而产生的杂交瘤细胞产生高亲和力抗体的高滴度的方法。 具有来自产生高亲和力抗体的杂交瘤的克隆抗体基因的杂交瘤细胞或哺乳动物表达细胞可能是错配修复缺陷，这是由于通过表达不匹配修复基因的显性负等位基因的内源性错配修复亚基的缺陷而允许杂交瘤 细胞可变性，可以通过化学方式使其变得过度，或者可能是天然错配修复缺陷。 可以通过本发明的方法制备产生抗体的高亲和力抗体和高滴度产生细胞。

    Anti-Mesothelin Antibodies
    50.
    发明申请
    Anti-Mesothelin Antibodies 有权
    抗间皮素抗体

    公开(公告)号：US20100028336A1

    公开(公告)日：2010-02-04

    申请号：US12545369

    申请日：2009-08-21

    申请人： Wolfgang Ebel ,  Luigi Grasso ,  Nicholas C. Nicolaides ,  Philip M. Sass ,  Eric Routhier

    发明人： Wolfgang Ebel ,  Luigi Grasso ,  Nicholas C. Nicolaides ,  Philip M. Sass ,  Eric Routhier

    IPC分类号： A61K39/395 ,  C07H21/04 ,  A61P35/00 ,  C12N15/74 ,  C12N5/16

    CPC分类号： C07K16/28 ,  A61K39/3955 ,  A61K45/06 ,  A61K2039/505 ,  C07K16/30 ,  C07K16/3069 ,  C07K2317/56 ,  C07K2317/732 ,  C07K2317/77

    摘要： This invention relates to the use of monoclonal and polyclonal antibodies that specifically bind to and become internalized by mesothelin-positive cells and also induce an immune effector activity such as antibody dependent cellular cytotoxicity. The antibodies are useful in specific delivery of pharmacologic agents to mesothelin expressing cells as well as eliciting an immune-effector activity particularly on tumor cells and precursors. The invention is also related to cells expressing the monoclonal antibodies, polyclonal antibodies, antibody derivatives, such as human, humanized, and chimeric monoclonal antibodies, antibody fragments, mammalian cells expressing the monoclonal antibodies, derivatives and fragments, and methods of treating cancer using the antibodies, derivatives and fragments.

    摘要翻译： 本发明涉及单克隆和多克隆抗体的使用，其特异性结合并被间皮素阳性细胞内化，并且还诱导免疫效应子活性如抗体依赖性细胞毒性。 该抗体可用于特异性递送表达间皮素的药物学药剂，以及引发免疫效应物特异性对肿瘤细胞和前体的活性。 本发明还涉及表达单克隆抗体，多克隆抗体，抗体衍生物如人，人源化和嵌合单克隆抗体的细胞，抗体片段，表达单克隆抗体的哺乳动物细胞，衍生物和片段，以及使用 抗体，衍生物和片段。