公开(公告)号：US20160326492A1

公开(公告)日：2016-11-10

申请号：US15110826

申请日：2015-01-12

Applicant: LABORATOIRES EXPANSCIENCE

Inventor： Stephanie BREDIF ,  Caroline BAUDOUIN ,  Philippe MSIKA ,  Marisa MELONI

IPC: C12N5/071 ,  G01N33/50 ,  C07K14/78

CPC classification number: C12N5/0629 ,  C07K14/495 ,  C07K14/52 ,  C07K14/78 ,  C12N5/0698 ,  C12N9/0006 ,  C12N2533/54 ,  G01N33/5082 ,  G01N33/5088 ,  G01N2333/47 ,  G01N2333/495 ,  G01N2333/525 ,  G01N2333/7055 ,  G01N2333/904

Abstract: A first subject of the invention is a skin model characterized in that it comprises keratinocytes capable of overexpressing filaggrin. A second subject of the invention is a method for evaluating the in vitro activity of a formulation or of at least one active agent on the healing of nipple skin and/or on the reduction of nipple skin inflammation, characterized in that said method comprises at least the following steps: a) bringing said active agent or said formulation into contact with a skin model comprising keratinocytes overexpressing filaggrin and also comprising a lesion; b) measuring the level of production of at least one biological marker in the nipple skin model of step a), characterized in that said biological marker can be chosen from lactate dehydrogenase (LDH), TNFα, filaggrin, TGFβ1 and β1 integrin; c) comparing the level of production of at least one biological marker obtained in step b) with a reference level of production; evaluating the efficacy of said active agent or of said formulation according to the comparison of step c).

Abstract translation: 本发明的第一主题是皮肤模型，其特征在于其包含能够过表达丝聚蛋白的角质形成细胞。 本发明的第二个主题是用于评估制剂或至少一种活性剂对乳头皮肤愈合和/或降低乳头皮肤炎症的体外活性的方法，其特征在于所述方法至少包括 以下步骤：a）使所述活性剂或所述制剂与包含过表达丝聚蛋白并且还包含病变的角质形成细胞的皮肤模型接触; b）测量步骤a）的乳头皮肤模型中至少一种生物标志物的产生水平，其特征在于所述生物标志物可以选自乳酸脱氢酶（LDH），TNFα，丝聚蛋白，TGFβ1和β1整联蛋白; c）将步骤b）中获得的至少一种生物标志物的产量水平与参考生产水平进行比较; 根据步骤c）的比较评估所述活性剂或所述制剂的功效。

公开(公告)号：US09439938B2

公开(公告)日：2016-09-13

申请号：US14370892

申请日：2013-01-14

Applicant: LABORATOIRES EXPANSCIENCE

Inventor： Philippe Msika ,  Jacques Legrand ,  Sebastien Garnier

IPC: A61K36/54 ,  A23L1/30 ,  A23D9/00 ,  A23D9/02 ,  C11B1/08 ,  A61K31/01 ,  A61K31/575

CPC classification number: A61K36/54 ,  A23D9/00 ,  A23D9/02 ,  A23L33/105 ,  A23L33/11 ,  A23L33/115 ,  A61K31/01 ,  A61K31/575 ,  C11B1/08

Abstract: The present invention relates to the use of avocado peels in order to obtain avocado unsaponifiable enriched in saturated aliphatic hydrocarbons and in sterols, said avocado peels accounting for 5 to 50% by weight relative to the total weight of avocado used. The invention also relates to a method for obtaining avocado unsaponifiable enriched in saturated aliphatic hydrocarbons and in sterols from at least avocado peels, said avocado peels accounting for 5 to 50% by weight relative to the total weight of avocado used. The invention also relates to an avocado unsaponifiable enriched in saturated aliphatic hydrocarbons and in sterols, obtainable by this method. Lastly, the invention relates to an avocado unsaponifiable enriched in saturated aliphatic hydrocarbons and in sterols, for use as a drug, advantageously in the in the prevention and/or treatment of conjunctive tissue disorders such as arthrosis, articular pathologies such as rheumatism, or periodontal diseases such as gingivitis or periodontitis.

Abstract translation: 本发明涉及鳄梨皮的使用以获得不可皂化的富含饱和脂肪烃的牛油果和甾醇，所述鳄梨皮相对于所用鳄梨的总重量占5至50重量％。 本发明还涉及一种用于从至少鳄梨皮中获得富含饱和脂肪烃和甾醇的鳄梨不可皂化的方法，所述鳄梨皮相对于所用牛油的总重量计算为5至50重量％。 本发明还涉及可通过该方法获得的不可皂化的富含饱和脂肪烃和甾醇的鳄梨。 最后，本发明涉及不可皂化的富含饱和脂族烃和固醇的鳄梨，用作药物，有利地用于预防和/或治疗结缔组织疾病如关节病，关节病变如风湿病或牙周病 疾病如牙龈炎或牙周炎。

公开(公告)号：US20160195515A1

公开(公告)日：2016-07-07

申请号：US14915702

申请日：2014-09-03

Applicant: LABORATOIRES EXPANSCIENCE

Inventor： Philippe Msika ,  Nadege Lachmann ,  Caroline Baudouin

IPC: G01N33/50 ,  G01N33/68

CPC classification number: G01N33/5044 ,  G01N33/5023 ,  G01N33/6881 ,  G01N2333/47

Abstract: The present invention thus relates to a method for determining the degree of maturation of the skin surface of a subject. In particular, the present invention relates to method for determining whether the skin surface of a subject is mature/immature in its organization/function, said method comprising the steps of taking a skin sample of the said subject; and determining the corneodesmosome distribution within the stratum corneum of the sample.

Abstract translation: 因此，本发明涉及一种用于确定受试者的皮肤表面的成熟程度的方法。 特别地，本发明涉及用于确定受试者的皮肤表面在其组织/功能中是否成熟/不成熟的方法，所述方法包括以下步骤：摄取所述受试者的皮肤样品; 并测定样品角质层内的角质层分布。

公开(公告)号：US20150285787A1

公开(公告)日：2015-10-08

申请号：US14414253

申请日：2013-07-15

Applicant: LABORATOIRES EXPANSCIENCE

Inventor： Philippe Msika ,  Caroline Baudouin

IPC: G01N33/50 ,  G01N33/92 ,  C12Q1/68 ,  G01N33/68

CPC classification number: G01N33/5044 ,  C12Q1/6876 ,  C12Q1/6888 ,  C12Q2600/148 ,  C12Q2600/158 ,  G01N33/5088 ,  G01N33/6881 ,  G01N33/6893 ,  G01N33/92 ,  G01N2800/20 ,  G01N2800/60

Abstract: The present invention relates to a method for identifying biological markers for characterizing children's skin. The method of the invention thus makes it possible to identify molecular markers that are expressed differently in children's skin than in the skin of adults. The present invention therefore enables the skin to be characterized at the molecular level from birth and the changes thereof with age to be tracked.

Abstract translation: 本发明涉及用于鉴定表征儿童皮肤的生物标志物的方法。 因此，本发明的方法使得可以鉴定在儿童皮肤中不同于在成人皮肤中表达的分子标记。 因此，本发明能够使皮肤在出生时的分子水平和随着年龄的变化被跟踪。

公开(公告)号：US20150005273A1

公开(公告)日：2015-01-01

申请号：US14370892

申请日：2013-01-14

Applicant: LABORATOIRES EXPANSCIENCE

Inventor： Philippe Msika ,  Jacques Legrand ,  Sebastien Garnier

IPC: A61K36/54 ,  A61K31/575 ,  A61K31/01

CPC classification number: A61K36/54 ,  A23D9/00 ,  A23D9/02 ,  A23L33/105 ,  A23L33/11 ,  A23L33/115 ,  A61K31/01 ,  A61K31/575 ,  C11B1/08

Abstract: The present invention relates to the use of avocado peels in order to obtain avocado unsaponifiable enriched in saturated aliphatic hydrocarbons and in sterols, said avocado peels accounting for 5 to 50% by weight relative to the total weight of avocado used. The invention also relates to a method for obtaining avocado unsaponifiable enriched in saturated aliphatic hydrocarbons and in sterols from at least avocado peels, said avocado peels accounting for 5 to 50% by weight relative to the total weight of avocado used. The invention also relates to an avocado unsaponifiable enriched in saturated aliphatic hydrocarbons and in sterols, obtainable by this method. Lastly, the invention relates to an avocado unsaponifiable enriched in saturated aliphatic hydrocarbons and in sterols, for use as a drug, advantageously in the in the prevention and/or treatment of conjunctive tissue disorders such as arthrosis, articular pathologies such as rheumatism, or periodontal diseases such as gingivitis or periodontitis.

Abstract translation: 本发明涉及鳄梨皮的使用以获得不可皂化的富含饱和脂肪烃的牛油果和甾醇，所述鳄梨皮相对于所用鳄梨的总重量占5至50重量％。 本发明还涉及一种用于从至少鳄梨皮中获得富含饱和脂肪烃和甾醇的鳄梨不可皂化的方法，所述鳄梨皮相对于所用牛油的总重量计算为5至50重量％。 本发明还涉及可通过该方法获得的不可皂化的富含饱和脂肪烃和甾醇的鳄梨。 最后，本发明涉及不可皂化的富含饱和脂族烃和固醇的鳄梨，用作药物，有利地用于预防和/或治疗结缔组织疾病如关节病，关节病变如风湿病或牙周病 疾病如牙龈炎或牙周炎。