公开(公告)号：US20250066851A1

公开(公告)日：2025-02-27

申请号：US18945323

申请日：2024-11-12

Applicant: 10x Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US20250043346A1

公开(公告)日：2025-02-06

申请号：US18919966

申请日：2024-10-18

Applicant: 10x Genomics, Inc.

Inventor： Paul Ryvkin ,  Jason Underwood ,  Michael Schnall-Levin ,  Tarjei Mikkelsen ,  Benjamin Hindson

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6851

Abstract: Provided herein are compositions, systems and methods for tagging molecular events, reactions, species, etc., but without the need for complex, highly diverse libraries of tagging molecules. Provided are tagging moieties that can have a smaller number, a few, or even a single original “tagging” structure that may be transformed or transformable, in situ, into a collection of larger numbers of unique tagging or “barcode” moieties.

公开(公告)号：US12163191B2

公开(公告)日：2024-12-10

申请号：US16898984

申请日：2020-06-11

Applicant: 10X GENOMICS, INC.

Inventor： Michael Schnall-Levin ,  Mirna Jarosz ,  Christopher Hindson ,  Kevin Ness ,  Serge Saxonov ,  Benjamin Hindson ,  Xinying Zheng ,  Patrick Marks ,  John Stuelpnagel

IPC: C12Q1/6883 ,  C12Q1/6827 ,  G16B30/00 ,  G16B30/10 ,  G16B30/20

Abstract: The present disclosure relates to methods, compositions and systems for haplotype phasing and copy number variation assays. Included within this disclosure are methods and systems for combining the barcode comprising beads with samples in multiple separate partitions, as well as methods of processing, sequencing and analyzing barcoded samples.

公开(公告)号：US20240376519A1

公开(公告)日：2024-11-14

申请号：US18384527

申请日：2023-10-27

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Donald Masquelier ,  Serge Saxonov ,  Landon Merrill ,  Andrew Price ,  Paul Hardenbol ,  Yuan Li

IPC: C12Q1/6806 ,  C12N15/10

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US11591637B2

公开(公告)日：2023-02-28

申请号：US16056231

申请日：2018-08-06

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Donald Masquelier ,  Serge Saxonov ,  Landon Merrill ,  Andrew Price ,  Paul Hardenbol ,  Yuan Li

IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/6806

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US11473138B2

公开(公告)日：2022-10-18

申请号：US16698740

申请日：2019-11-27

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Mirna Jarosz ,  Paul Hardenbol ,  Michael Schnall-Levin ,  Kevin Ness ,  Serge Saxonov

IPC: C12Q1/6874 ,  C12Q1/683 ,  C12N15/00 ,  C12N15/10 ,  C12Q1/6806

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing. In some cases, this disclosure provides methods for the generation of polynucleotide barcode libraries, and for the attachment of such polynucleotides to target polynucleotides.

公开(公告)号：US11278901B2

公开(公告)日：2022-03-22

申请号：US16887024

申请日：2020-05-29

Applicant: 10X Genomics, Inc.

Inventor： Rajiv Bharadwaj ,  Tobias Daniel Wheeler ,  Kevin Ness ,  Benjamin Hindson

IPC: B01L3/00 ,  B01J2/06 ,  C12Q1/6844 ,  B01F13/00 ,  B01F3/08

Abstract: A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.

公开(公告)号：US20210324457A1

公开(公告)日：2021-10-21

申请号：US17271866

申请日：2019-08-27

Applicant: Eswar Prasad RAMACHANDRAN IYER ,  Michael SCHNALL-LEVIN ,  Augusto Manuel TENTORI ,  Tarje Sigurd MIKKELSEN ,  Benjamin HINDSON ,  Michael Ybarra LUCERO ,  Rajiv BHARADWAJ ,  Yifeng YIN ,  Zachary BENT ,  Jennifer CHEW ,  Meghan L. F. FREY ,  Linda KVASTAD ,  Joakim LUNDEBERG ,  Eva Gracia VILLACAMPA ,  10x Genomics, Inc.

Inventor： Eswar Prasad Ramachandran Iyer ,  Michael Schnall-Levin ,  Augusto Manuel Tentori ,  Tarjei Sigurd Mikkelsen ,  Benjamin Hindson ,  Michael Ybarra Lucero ,  Rajiv Bharadwaj ,  Yifeng Yin ,  Zachary Bent ,  Jennifer Chew ,  Meghan L. F. Frey ,  Linda Kvastad ,  Joakim Lundeberg ,  Eva Gracia Villacampa

IPC: C12Q1/6837 ,  C12Q1/6841

Abstract: This disclosure relates to methods for generating spatially barcoded arrays using concentration gradients.

公开(公告)号：US20210277465A1

公开(公告)日：2021-09-09

申请号：US17314526

申请日：2021-05-07

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol

IPC: C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20210123103A1

公开(公告)日：2021-04-29

申请号：US16898984

申请日：2020-06-11

Applicant: 10X GENOMICS, INC.

Inventor： Michael Schnall-Levin ,  Mirna Jarosz ,  Christopher Hindson ,  Kevin Ness ,  Serge Saxonov ,  Benjamin Hindson ,  Xinying Zheng ,  Patrick Marks ,  John Stuelpnagel

IPC: C12Q1/6883 ,  G16B30/00 ,  C12Q1/6827

Abstract: The present disclosure relates to methods, compositions and systems for haplotype phasing and copy number variation assays. Included within this disclosure are methods and systems for combining the barcode comprising beads with samples in multiple separate partitions, as well as methods of processing, sequencing and analyzing barcoded samples.