公开(公告)号：US06586215B2

公开(公告)日：2003-07-01

申请号：US09885329

申请日：2001-06-19

申请人： Debbie Yaver ,  Barbara McArdle

发明人： Debbie Yaver ,  Barbara McArdle

IPC分类号： C12N908

CPC分类号： C12N9/0065

摘要： The present invention relates to isolated polypeptides having peroxidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

公开(公告)号：US09493790B2

公开(公告)日：2016-11-15

申请号：US14238691

申请日：2012-08-23

申请人： Debbie Yaver ,  Qiming Jin

发明人： Debbie Yaver ,  Qiming Jin

IPC分类号： C12N15/80 ,  C12N9/24 ,  C12N15/87 ,  C12N9/42

CPC分类号： C12N9/2437 ,  C12N9/2402 ,  C12N15/80 ,  C12N15/87 ,  C12Y302/01091 ,  C12Y302/0115

摘要： The present invention relates to methods for constructing a filamentous fungal strain for production of multiple recombinant polypeptides having biological activity. The present invention also relates to methods for producing multiple recombinant polypeptides having biological activity in a filamentous fungal strain. The present invention also relates to filamentous fungal strains expressing multiple recombinant polypeptides having biological activity.

摘要翻译： 本发明涉及构建用于生产具有生物活性的多重重组多肽的丝状真菌菌株的方法。 本发明还涉及在丝状真菌菌株中生产具有生物活性的多重重组多肽的方法。 本发明还涉及表达具有生物活性的多种重组多肽的丝状真菌菌株。

公开(公告)号：US20130302865A1

公开(公告)日：2013-11-14

申请号：US13948945

申请日：2013-07-23

申请人： Amanda Fischer ,  Debbie Yaver

发明人： Amanda Fischer ,  Debbie Yaver

IPC分类号： C12P7/46 ,  C12N15/80

CPC分类号： C12P7/46 ,  C07K14/38 ,  C12N9/0006 ,  C12N9/88 ,  C12N15/80 ,  C12Y604/01001

摘要： The present invention relates to isolated polypeptides having C4-dicarboxylic acid transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.

摘要翻译： 本发明涉及具有C4-二羧酸转运蛋白活性的分离多肽和编码该多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法，以及生产C4-二羧酸如苹果酸的方法。

公开(公告)号：US08518676B2

公开(公告)日：2013-08-27

申请号：US13404258

申请日：2012-02-24

申请人： Amanda Fischer ,  Debbie Yaver

发明人： Amanda Fischer ,  Debbie Yaver

IPC分类号： C12N15/31 ,  C12N1/15 ,  C12P7/46

CPC分类号： C12P7/46 ,  C07K14/38 ,  C12N9/0006 ,  C12N9/88 ,  C12N15/80 ,  C12Y604/01001

摘要： The present invention relates to isolated polypeptides having C4-dicarboxylic acid transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.

摘要翻译： 本发明涉及具有C4-二羧酸转运蛋白活性的分离多肽和编码多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法，以及生产C4-二羧酸如苹果酸的方法。

公开(公告)号：US08273348B2

公开(公告)日：2012-09-25

申请号：US12519868

申请日：2007-12-12

申请人： Allan Svendsen ,  Michael Skjoet ,  Debbie Yaver ,  Lars Lehmann Hylling Christensen ,  Signe Eskildsen Larsen ,  Nina Lundin ,  Michael Lamsa ,  Peter Colin Gregory

发明人： Allan Svendsen ,  Michael Skjoet ,  Debbie Yaver ,  Lars Lehmann Hylling Christensen ,  Signe Eskildsen Larsen ,  Nina Lundin ,  Michael Lamsa ,  Peter Colin Gregory

IPC分类号： A61K38/54 ,  A61K38/46 ,  C12Q1/34 ,  C12Q1/40 ,  C12N9/20 ,  C12N9/14 ,  C12N9/26 ,  C07H21/04 ,  C07K1/00

CPC分类号： A61K38/465 ,  A61K38/47 ,  A61K38/48 ,  C12N9/20

摘要： The pharmaceutical use of lipases related to the Thermomyces lanuginosus (Humicola lanuginosa) lipase comprising amino acids 1-269 of SEQ ID NO: 2, optionally in combination with a protease and/or an amylase. Examples of medical indications are: Treatment of digestive disorders, pancreatic exocrine insufficiency (PEI), pancreatitis, cystic fibrosis, diabetes type I, and/or diabetes type II. The lipases of the invention have, e.g., an improved digestion performance in vitro, an improved activity at a pH in the neutral range, an improved stability at low pH, an are stable against protease-degradation, and/or are stable in the presence of pepsin and bile salts. The invention also relates to methods of determining digestion performance in vitro of lipases, as well as to certain novel variants of the lipase of T. lanuginosus.

摘要翻译： 与包含SEQ ID NO：2的氨基酸1-269的Thermomyces lanuginosus（Humicola lanuginosa）脂肪酶相关的脂肪酶的药物用途，任选地与蛋白酶和/或淀粉酶组合。 医学适应症的例子有：消化系统疾病，胰腺外分泌功能不全（PEI），胰腺炎，囊性纤维化，I型糖尿病和/或II型糖尿病的治疗。 本发明的脂肪酶具有例如体外改进的消化性能，在中性范围内的pH值改善的活性，在低pH下的改进的稳定性，对蛋白酶降解是稳定的，和/或在存在时是稳定的 的胃蛋白酶和胆汁盐。 本发明还涉及确定脂肪酶的体外消化性能的方法以及T.lanuginosus的脂肪酶的某些新颖变体。

公开(公告)号：US08158395B2

公开(公告)日：2012-04-17

申请号：US13165696

申请日：2011-06-21

申请人： Amanda Fischer ,  Debbie Yaver

发明人： Amanda Fischer ,  Debbie Yaver

IPC分类号： C12N15/31 ,  C12N1/15 ,  C12P7/46

CPC分类号： C12P7/46 ,  C07K14/38 ,  C12N9/0006 ,  C12N9/88 ,  C12N15/80 ,  C12Y604/01001

摘要： The present invention relates to isolated polypeptides having C4-dicarboxylic acid transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.

摘要翻译： 本发明涉及具有C4-二羧酸转运蛋白活性的分离多肽和编码该多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法，以及生产C4-二羧酸如苹果酸的方法。

公开(公告)号：US20110239333A1

公开(公告)日：2011-09-29

申请号：US13151128

申请日：2011-06-01

申请人： Debbie Yaver ,  Paul Harris ,  Suzanne Otani ,  Janine Lin ,  Haiyan Ge

发明人： Debbie Yaver ,  Paul Harris ,  Suzanne Otani ,  Janine Lin ,  Haiyan Ge

IPC分类号： A01H5/00 ,  C07H21/00 ,  C07H21/04 ,  C12N15/63 ,  C12N1/00 ,  C12N5/10 ,  C12N9/24

CPC分类号： C12N9/2482 ,  C12N9/248 ,  C12Y302/01008

摘要： The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

摘要翻译： 本发明涉及具有木聚糖酶活性的分离的多肽和编码多肽的分离的多核苷酸。 本发明还涉及核酸构建体，载体，和包含所述多核苷酸的宿主细胞以及方法用于产生和使用所述多肽。

公开(公告)号：US20090217463A1

公开(公告)日：2009-09-03

申请号：US12393115

申请日：2009-02-26

申请人： Philip Frank Souter ,  Neil Joseph Lant ,  Theresa Clare Haynes ,  Jesper Vind ,  Jurgen Carsten Franz Knotzel ,  Kim Borch ,  Allan Svendsen ,  Thomas Honger Callisen ,  Debbie Yaver ,  Mads Eskelund Bjornvad ,  Peter Kamp Hansen

发明人： Philip Frank Souter ,  Neil Joseph Lant ,  Theresa Clare Haynes ,  Jesper Vind ,  Jurgen Carsten Franz Knotzel ,  Kim Borch ,  Allan Svendsen ,  Thomas Honger Callisen ,  Debbie Yaver ,  Mads Eskelund Bjornvad ,  Peter Kamp Hansen

IPC分类号： C11D3/386 ,  C12S11/00 ,  C11D3/50

CPC分类号： C12N9/20 ,  C11D3/38627

摘要： This invention relates to compositions comprising certain lipase enzymes and processes for making and using such compositions, including the use of such compositions to clean and/or treat a situs.

摘要翻译： 本发明涉及包含某些脂肪酶的组合物和制备和使用这些组合物的方法，包括使用这种组合物来清洁和/或治疗部位。

公开(公告)号：US20090029440A1

公开(公告)日：2009-01-29

申请号：US12161582

申请日：2007-01-22

申请人： Jesper Vind ,  Jurgen Carsten Franz ,  Kim Borch ,  Allan Svendsen ,  Thomas Honger Callisen ,  Debbie Yaver ,  Mads Eskelund Bjornvad ,  Peter Kamp Hansen ,  Haiyan Ge ,  Michael Lamsa

发明人： Jesper Vind ,  Jurgen Carsten Franz ,  Kim Borch ,  Allan Svendsen ,  Thomas Honger Callisen ,  Debbie Yaver ,  Mads Eskelund Bjornvad ,  Peter Kamp Hansen ,  Haiyan Ge ,  Michael Lamsa

IPC分类号： C12N9/20 ,  C12N15/55 ,  C12N1/15 ,  C12N1/21

CPC分类号： C12N9/20 ,  C12N9/18

摘要： The invention provides variant lipases, preferably, variants with reduced tendency to odor generation obtained by introducing mutations in one or more regions identified in a parent lipase.

摘要翻译： 本发明提供了变体脂肪酶，优选具有通过在母体脂肪酶中鉴定的一个或多个区域中引入突变获得的具有降低的气味产生倾向的变体。

公开(公告)号：US20080274500A1

公开(公告)日：2008-11-06

申请号：US12054125

申请日：2008-03-24

申请人： Debbie Yaver ,  Peter Nham

发明人： Debbie Yaver ,  Peter Nham

IPC分类号： C12P21/06 ,  C07H21/04 ,  C12N15/00 ,  C12N1/15

CPC分类号： C12N15/80 ,  C12N9/0044 ,  C12N9/20 ,  C12N9/58 ,  C12N9/93 ,  C12N15/113 ,  C12P1/02 ,  C12P21/00 ,  C12Y107/01003 ,  C12Y301/01003 ,  C12Y304/21004 ,  C12Y603/04002

摘要： The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, and SEQ ID NO: 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.

摘要翻译： 本发明涉及生物体的制造方法，其特征在于，包括：（a）在有助于生物生物的培养基中培养真菌宿主细胞，其中所述真菌宿主细胞可操作地包含编码生物物质的第一核酸序列 连接到第二核酸序列，其包含选自SEQ ID NO：2，SEQ ID NO：3，SEQ ID NO：4，SEQ ID NO：5，SEQ ID NO：6，SEQ ID NO： ：7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10，SEQ ID NO：11和SEQ ID NO：12; 及其后果; 及其杂交和串联启动子; 和（b）从培养基中分离生物物质。 本发明还涉及分离的启动子变体和构建体，载体和真菌宿主细胞，其包含可操作地连接于编码生物物质的核酸序列的启动子变体。