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    Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby
    51.
    发明授权
    Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby 有权
    用于在由其制造的氧化物层和制品上形成钝化表面的中间体和方法

    公开(公告)号：US07928038B2

    公开(公告)日：2011-04-19

    申请号：US11943851

    申请日：2007-11-21

    申请人： Steven M. Menchen ,  Christina E. Inman ,  Meng Taing ,  Khai Luong ,  Handong Li

    发明人： Steven M. Menchen ,  Christina E. Inman ,  Meng Taing ,  Khai Luong ,  Handong Li

    IPC分类号： C40B60/14 ,  C12M1/34 ,  C07H21/02 ,  A61K38/04

    CPC分类号： G01N33/54393 ,  G01N33/551 ,  Y10T428/31678

    摘要： Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby are described. Hydroxyl or hydroxide groups on the oxide surfaces are reacted with a metal reagent of the formula Y(L-Pol)m, where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and “Pol” represents a passivating agent such as a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a polyvalent reagent comprising a passivating moiety and a plurality of functional groups that are reactive with or that form complexes with Y. The passivating agent can also include a functional group such as biotin to provide surfaces with a desired functionality. The passivated surfaces exhibit minimal binding to bio-molecules and can be used in single-molecule detection schemes.

    摘要翻译： 描述了用于在氧化物层上形成钝化表面的中间体和方法以及由此制备的制品。 氧化物表面上的羟基或氢氧基与式Y（L-Pol）m的金属试剂反应，其中Y是过渡金属，镁或铝，L是氧，硫，硒或胺，“Pol “表示钝化剂，例如聚乙二醇，烃或碳氟化合物。 所得到的改性表面可以进一步与具有磷酸酯官能团的钝化剂或包含钝化部分和多个与Y形成复合物的多个官能团的多价试剂反应。钝化剂还可以包括官能团 例如生物素，以提供具有所需功能的表面。 被钝化的表面表现出与生物分子的最小结合，并可用于单分子检测方案。

    Mobility-Modifying Cyanine Dyes
    58.
    发明授权
    Mobility-Modifying Cyanine Dyes 有权
    流动性改性花青染料

    公开(公告)号：US06716994B1

    公开(公告)日：2004-04-06

    申请号：US09477270

    申请日：2000-01-04

    申请人： Steven M. Menchen ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Shaheer H. Khan

    发明人： Steven M. Menchen ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Shaheer H. Khan

    IPC分类号： C07D20902

    CPC分类号： C07H21/00 ,  C07F9/2408 ,  C09B23/02 ,  G01N27/44726

    摘要： The present invention provides a novel class of fluorescent cyanine dye compounds that are modified at one of the hetercyclic ring nitrogen atoms with a mobility-modifying moiety that permits the electrophoretic mobilities of polynucleotides labeled with the mobility-modifying cyanine dyes to be adjusted or tuned in a predictable fashion while retaining enzymatic activity. The ability to predictably tune the relative electrophoretic mobilities of the dyes permits the creation of sets of mobility-matched fluorescent dyes of a variety of structures for a variety of applications, including fluorescence-based 4-color nucleic acid sequencing reactions.

    摘要翻译： 本发明提供了一类新颖的荧光花青染料化合物，其在一个杂环氮原子上被修饰，具有迁移率调节部分，其允许调节或调谐用迁移率调节花青染料标记的多核苷酸的电泳迁移率 一种可预测的方式，同时保留酶活性。 可预测地调节染料的相对电泳迁移率的能力允许为各种应用（包括基于荧光的4色核酸测序反应）创建各种结构的迁移率匹配荧光染料组。