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公开(公告)号：US07018431B2
公开(公告)日：2006-03-28
申请号：US10602974
申请日：2003-06-24
申请人： Steven M. Menchen , Scott C. Benson , Joe Y. L. Lam , Weiguo Zhen , Daqing Sun , Barnett B. Rosenblum , Shaheer H. Khan , Meng Taing
发明人： Steven M. Menchen , Scott C. Benson , Joe Y. L. Lam , Weiguo Zhen , Daqing Sun , Barnett B. Rosenblum , Shaheer H. Khan , Meng Taing
IPC分类号： C07D311/78
CPC分类号： C07D311/78 , C07D209/60 , C07D221/10 , C07D311/94 , C07D491/14 , C07H19/10 , C07H19/20 , C07H21/00 , C09B11/02 , C09B11/24
摘要： Sulfonated diarylrhodamine compounds are useful as fluorescent labels of nucleosides, nucleotides, polynucleotides, and polypeptides. The compounds find particular application in the area of fluorescent nucleic acid analysis, e.g., automated DNA sequencing and fragment analysis, detection of probe hybridization in hybridization arrays, detection of nucleic acid amplification products, and the like.
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公开(公告)号：US06583168B1
公开(公告)日：2003-06-24
申请号：US09724855
申请日：2000-11-28
申请人： Steven M. Menchen , Scott C. Benson , Joe Y. L. Lam , Weiguo Zhen , Daqing Sun , Barnett B. Rosenblum , Shaheer H. Khan , Meng Taing
发明人： Steven M. Menchen , Scott C. Benson , Joe Y. L. Lam , Weiguo Zhen , Daqing Sun , Barnett B. Rosenblum , Shaheer H. Khan , Meng Taing
IPC分类号： C07D31178
CPC分类号： C07D311/78 , C07D209/60 , C07D221/10 , C07D311/94 , C07D491/14 , C07H19/10 , C07H19/20 , C07H21/00 , C09B11/02 , C09B11/24
摘要： Sulfonated diarylrhodamine compounds are useful as fluorescent labels of nucleosides, nucleotides, polynucleotides, and polypeptides. The compounds find particular application in the area of fluorescent nucleic acid analysis, e.g., automated DNA sequencing and fragment analysis, detection of probe hybridization in hybridization arrays, detection of nucleic acid amplification products, and the like.
摘要翻译： 磺化二芳基罗丹明化合物可用作核苷，核苷酸，多核苷酸和多肽的荧光标记。 该化合物在荧光核酸分析的领域中具体应用，例如自动DNA测序和片段分析，杂交阵列中探针杂交的检测，核酸扩增产物的检测等。
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公开(公告)号：US06811979B2
公开(公告)日：2004-11-02
申请号：US09976168
申请日：2001-10-11
IPC分类号： C12Q168
摘要： Provided are nucleotide-dye conjugates and related compounds in which a dye is linked to a nucleobase directly or indirectly by an anionic linker. The anionic character of the linker is provided by one or more anionic moieties which are present in the linker, such as phosphate, phosphonate, sulfonate, and carboxylate groups. When the dye is a provided as a donor/acceptor dye pair, the anionic linker can be located between the donor and the acceptor, or between the nucleobase and either the donor or acceptor, or both. In one embodiment, conjugates of the invention provide enhanced electrophoretic mobility characteristics to sequencing fragments, e.g., for dideoxy sequencing using labeled terminators.
摘要翻译： 提供的是核苷酸 - 染料缀合物和相关化合物，其中染料通过阴离子连接体直接或间接连接到核碱基。 连接体的阴离子特征由连接物中存在的一个或多个阴离子部分提供，例如磷酸酯，膦酸酯，磺酸酯和羧酸酯基团。 当染料作为供体/受体染料对提供时，阴离子连接体可以位于供体和受体之间，或位于核碱基与供体或受体之间或两者之间。 在一个实施方案中，本发明的缀合物为测序片段提供增强的电泳迁移率特性，例如使用标记的终止子进行双脱氧测序。
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公开(公告)号：US20100304381A1
公开(公告)日：2010-12-02
申请号：US12701423
申请日：2010-02-05
摘要： Provided are nucleotide-dye conjugates and related compounds in which a dye is linked to a nucleobase directly or indirectly by an anionic linker. The anionic character of the linker is provided by one or more anionic moieties which are present in the linker, such as phosphate, phosphonate, sulfonate, and carboxylate groups. When the dye is a provided as a donor/acceptor dye pair, the anionic linker can be located between the donor and the acceptor, or between the nucleobase and either the donor or acceptor, or both. In one embodiment, conjugates of the invention provide enhanced electrophoretic mobility characteristics to sequencing fragments, e.g., for dideoxy sequencing using labeled terminators.
摘要翻译： 提供的是核苷酸 - 染料缀合物和相关化合物，其中染料通过阴离子连接体直接或间接连接到核碱基。 连接体的阴离子特征由连接物中存在的一个或多个阴离子部分提供，例如磷酸酯，膦酸酯，磺酸酯和羧酸酯基团。 当染料作为供体/受体染料对提供时，阴离子连接体可以位于供体和受体之间，或位于核碱基与供体或受体之间或两者之间。 在一个实施方案中，本发明的缀合物为测序片段提供增强的电泳迁移率特性，例如使用标记的终止子进行双脱氧测序。
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公开(公告)号：US07429651B2
公开(公告)日：2008-09-30
申请号：US10977341
申请日：2004-10-28
IPC分类号： C07H21/04
摘要： Provided are nucleotide-dye conjugates and related compounds in which a dye is linked to a nucleobase directly or indirectly by an anionic linker. The anionic character of the linker is provided by one or more anionic moieties which are present in the linker, such as phosphate, phosphonate, sulfonate, and carboxylate groups. When the dye is a provided as a donor/acceptor dye pair, the anionic linker can be located between the donor and the acceptor, or between the nucleobase and either the donor or acceptor, or both. In one embodiment, conjugates of the invention provide enhanced electrophoretic mobility characteristics to sequencing fragments, e.g., for dideoxy sequencing using labeled terminators.
摘要翻译： 提供的是核苷酸 - 染料缀合物和相关化合物，其中染料通过阴离子连接体直接或间接连接到核碱基。 连接体的阴离子特征由连接物中存在的一个或多个阴离子部分提供，例如磷酸酯，膦酸酯，磺酸酯和羧酸酯基团。 当染料作为供体/受体染料对提供时，阴离子连接体可以位于供体和受体之间，或位于核碱基与供体或受体之间或两者之间。 在一个实施方案中，本发明的缀合物为测序片段提供增强的电泳迁移率特性，例如使用标记的终止子进行双脱氧测序。
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公开(公告)号：US5948648A
公开(公告)日：1999-09-07
申请号：US87250
申请日：1998-05-29
IPC分类号： C07H19/04 , C07H19/06 , C07H19/14 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/68 , C12P19/40
摘要： A nucleoside/tide compound having the structureNUC--L--S--LB/LGis described wherein NUC is a nucleoside/tide having a nucleobase portion B, L is a rigid linkage, S is a spacer; and LB/LG is a member of a linkage pair or a label. NUC is attached to L through B such that when B is a purine, L is attached to the 8-position of the purine, when B is 7-deazapurine, L is attached to the 7-position of the 7-deazapurine, and when B is pyrimidine, L is attached to the 5-position of the pyrimidine. In an important aspect of the invention, L has the structure ##STR1## wherein each of n, o and p are integers ranging from 0 to 3, and the sum of n, o and p is at least 2, and each of W, X, Y and Z is selected from the group consisting of carbon and nitrogen. The invention further includes polynucleotide compounds comprising the nuclcoside/tide, and primer extension methods utilizing the nucleoside/tide, particularly when used in combination with certain mutant polymerase enzymes.
摘要翻译： 其中NUC为具有核碱基部分B的核苷/潮汐，L为刚性键，S为间隔基，具有结构为NUC-L-S-LB / LG的核苷/潮汐化合物。 而LB / LG是连锁对或标签的成员。 N与B连接，B为嘌呤时，L为嘌呤8位时，B为7-脱氮嘌呤时，L为7-脱氮嘌呤的7-位， B是嘧啶，L连接到嘧啶的5位上。 在本发明的一个重要方面，L具有其中n，o和p各自为0〜3的整数，n，o和p的和为2以上的结构，W，X，Y Z选自碳和氮。 本发明还包括包含核苷/潮汐的多核苷酸化合物，以及利用核苷/潮汐的引物延伸方法，特别是当与某些突变型聚合酶组合使用时。
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公开(公告)号：US06197555B1
公开(公告)日：2001-03-06
申请号：US09461510
申请日：1999-12-14
IPC分类号： C12P1934
CPC分类号： C07H19/06 , C07H19/04 , C07H19/10 , C07H19/14 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/6869 , C12Q2535/101
摘要： A nucleoside/tide compound having the structure NUC-L-S-LB/LG is described wherein NUC is a nucleoside/tide having a nucleobase portion B, L is a rigid linkage, S is a spacer; and LB/LG is a member of a linkage pair or a label. NUC is attached to L through B such that when B is a purine, L is attached to the 8-position of the purine, when B is 7-deazapurine, L is attached to the 7-position of the 7-deazapurine, and when B is pyrimidine, L is attached to the 5-position of the pyrimidine. In an important aspect of the invention, L has the structure wherein each of n, o and p are integers ranging from 0 to 3, and the sum of n, o and p is at least 2, and each of W, X, Y and Z is selected from the group consisting of carbon and nitrogen. The invention further includes polynucleotide compounds comprising the nucleoside/tide, and primer extension methods utilizing the nucleoside/tide, particularly when used in combination with certain mutant polymerase enzymes.
摘要翻译： 具有所述结构的核苷/潮汐化合物，其中NUC是具有核碱基部分B的核苷/ /，L是刚性键，S是间隔基; 而LB / LG是连锁对或标签的成员。 N与B连接，B为嘌呤时，L为嘌呤8位时，B为7-脱氮嘌呤时，L为7-脱氮嘌呤的7-位， B是嘧啶，L连接到嘧啶的5位上。 在本发明的一个重要方面，L具有其中n，o和p各自为0至3的整数，n，o和p之和至少为2，W，X，Y和 Z选自碳和氮。 本发明还包括包含核苷/潮汐的多核苷酸化合物，以及利用核苷/潮汐的引物延伸方法，特别是当与某些突变型聚合酶组合使用时。
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公开(公告)号：US06653462B2
公开(公告)日：2003-11-25
申请号：US10085561
申请日：2002-02-26
IPC分类号： C07H2100
CPC分类号： C07H21/00 , C07H19/04 , C07H19/06 , C07H19/14 , C07H19/16 , C07H19/20 , C12Q1/6869 , C12Q2525/186 , C12Q2525/117 , C12Q2525/113
摘要： A nucleoside/tide compound having the structure: NUC-L-S-LB/LG is described wherein NUC is a nucleoside/tide having a nucleobase portion B, L is a rigid linkage, S is a spacer; and LB/LG is a member of a linkage pair or a label. NUC is attached to L through B such that when B is a purine, L is attached to the 8-position of the purine, when B is 7-deazapurine, L is attached to the 7-position of the 7-deazapurine, and when B is pyrimidine, L is attached to the 5-position of the pyrimidine. In an important aspect of the invention, L has the structure: wherein each of n, o and p are integers ranging from 0 to 3, and the sum of n, o and p is at least 2, and each of W, X, Y and Z is selected from the group consisting of carbon and nitrogen. The invention further includes polynucleotide compounds comprising the nucleoside/tide, and primer extension methods utilizing the nucleoside/tide, particularly when used in combination with certain mutant polymerase enzymes.
摘要翻译： 描述了具有以下结构的核苷/潮汐化合物：其中NUC是具有核碱基部分B的核苷/潮汐，L是刚性键，S是间隔基; 而LB / LG是连锁对或标签的成员。 N与B连接，B为嘌呤时，L为嘌呤8位时，B为7-脱氮嘌呤时，L为7-脱氮嘌呤的7-位， B是嘧啶，L连接到嘧啶的5位上。 在本发明的一个重要方面，L具有以下结构：其中n，o和p各自为0至3的整数，n，o和p之和至少为2，W，X， Y和Z选自碳和氮。 本发明还包括包含核苷/潮汐的多核苷酸化合物，以及利用核苷/潮汐的引物延伸方法，特别是当与某些突变型聚合酶组合使用时。
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公开(公告)号：US6096875A
公开(公告)日：2000-08-01
申请号：US172789
申请日：1998-10-14
IPC分类号： C12N15/09 , C07F9/6558 , C07H19/04 , C07H19/06 , C07H19/073 , C07H19/10 , C07H19/14 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/68
CPC分类号： C07H19/06 , C07H19/04 , C07H19/10 , C07H19/14 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/6869 , C12Q2535/101
摘要： A nucleoside/tide compound having the structureNUC--L--S--LB/LGis described wherein NUC is a nucleoside/tide having a nucleobase portion B, L is a rigid linkage, S is a spacer; and LB/LG is a member of a linkage pair or a label. NUC is attached to L through B such that when B is a purine, L is attached to the 8-position of the purine, when B is 7-deazapurine, L is attached to the 7-position of the 7-deazapurine, and when B is pyrimidine, L is attached to the 5-position of the pyrimidine. In an important aspect of the invention, L has the structure ##STR1## wherein each of n, o and p are integers ranging from 0 to 3, and the sum of n, o and p is at least 2, and each of W, X, Y and Z is selected from the group consisting of carbon and nitrogen. The invention further includes polynucleotide compounds comprising the nucleoside/tide, and primer extension methods utilizing the nucleoside/tide, particularly when used in combination with certain mutant polymerase enzymes.
摘要翻译： 其中NUC为具有核碱基部分B的核苷/潮汐，L为刚性键，S为间隔基，具有结构为NUC-L-S-LB / LG的核苷/ 而LB / LG是连锁对或标签的成员。 N与B连接，B为嘌呤时，L为嘌呤8位时，B为7-脱氮嘌呤时，L为7-脱氮嘌呤的7-位， B是嘧啶，L连接到嘧啶的5位上。 在本发明的一个重要方面，L具有其中n，o和p各自为0〜3的整数，n，o和p的和为2以上的结构，W，X，Y Z选自碳和氮。 本发明还包括包含核苷/潮汐的多核苷酸化合物，以及利用核苷/潮汐的引物延伸方法，特别是当与某些突变型聚合酶组合使用时。
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公开(公告)号：US06716994B1
公开(公告)日：2004-04-06
申请号：US09477270
申请日：2000-01-04
IPC分类号： C07D20902
CPC分类号： C07H21/00 , C07F9/2408 , C09B23/02 , G01N27/44726
摘要： The present invention provides a novel class of fluorescent cyanine dye compounds that are modified at one of the hetercyclic ring nitrogen atoms with a mobility-modifying moiety that permits the electrophoretic mobilities of polynucleotides labeled with the mobility-modifying cyanine dyes to be adjusted or tuned in a predictable fashion while retaining enzymatic activity. The ability to predictably tune the relative electrophoretic mobilities of the dyes permits the creation of sets of mobility-matched fluorescent dyes of a variety of structures for a variety of applications, including fluorescence-based 4-color nucleic acid sequencing reactions.
摘要翻译： 本发明提供了一类新颖的荧光花青染料化合物，其在一个杂环氮原子上被修饰，具有迁移率调节部分，其允许调节或调谐用迁移率调节花青染料标记的多核苷酸的电泳迁移率 一种可预测的方式，同时保留酶活性。 可预测地调节染料的相对电泳迁移率的能力允许为各种应用（包括基于荧光的4色核酸测序反应）创建各种结构的迁移率匹配荧光染料组。
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