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    Fungal Alpha-Amylase Variants
    51.
    发明申请
    Fungal Alpha-Amylase Variants 审中-公开
    真菌α-淀粉酶变体

    公开(公告)号：US20070264700A1

    公开(公告)日：2007-11-15

    申请号：US10536101

    申请日：2004-08-23

    申请人： Allan Svendsen ,  Lars Beier ,  Jesper Vind ,  Tina Spendler ,  Morten Jensen

    发明人： Allan Svendsen ,  Lars Beier ,  Jesper Vind ,  Tina Spendler ,  Morten Jensen

    IPC分类号： C12N9/30

    CPC分类号： C12N9/2417 ,  A21D2/267 ,  A21D8/042 ,  C12N9/242

    摘要： The inventors have developed a method of altering the amino acid sequence of a fungal alpha-amylase to obtain variants, and they have used the method to construct such variants. The variants may be useful for anti-staling in baked products. Accordingly, the invention provides a method of constructing fungal alpha-amylase variants based on a comparison of three-dimensional (3D) structures of the fungal alpha-amylase and a maltogenic alpha-amylase. One or both models includes a substrate. The invention also provides novel fungal alpha-amylase variants.

    摘要翻译： 发明人已经开发了改变真菌α-淀粉酶的氨基酸序列以获得变体的方法，并且已经使用该方法来构建这些变体。 这些变体可用于烘焙产品中的防老化。 因此，本发明提供了基于真菌α-淀粉酶和产麦芽糖α-淀粉酶的三维（3D）结构的比较来构建真菌α-淀粉酶变体的方法。 一种或两种型号包括基材。 本发明还提供了新的真菌α-淀粉酶变体。

    Human heavy chain antibody expression in flamentous fungi
    53.
    发明申请
    Human heavy chain antibody expression in flamentous fungi 审中-公开
    人重链抗体在丝状真菌中的表达

    公开(公告)号：US20060234340A1

    公开(公告)日：2006-10-19

    申请号：US10544302

    申请日：2004-02-06

    申请人： Jesper Vind

    发明人： Jesper Vind

    IPC分类号： C12P21/06 ,  C07H21/04 ,  C07K16/44 ,  C12N1/16 ,  C12N15/74

    CPC分类号： C07K16/32 ,  C07K16/00 ,  C07K2317/21 ,  C07K2317/22 ,  C07K2317/24 ,  C07K2317/56

    摘要： The present invention relates to a method for producing a functional human immunoglobulin, wherein a human heavy chain immunoglobulin, devoid of any light chain, is expressed, comprising the steps of: a) transforming a filamentous host cell with a recombinant construct encoding a modified human heavy chain immunoglobulin, wherein the modifications comprise one or more mutations in the region of the heavy chain protein involved in contact with the light chain; b) culturing said filamentous host cell under conditions promoting expression of said modified human heavy chain immunoglobulin; and c) recovering said modified human heavy chain immunoglobulin.

    摘要翻译： 本发明涉及一种功能性人免疫球蛋白的制备方法，其中表达没有任何轻链的人重链免疫球蛋白，包括以下步骤：a）用编码修饰的人的重组构建体转化丝状宿主细胞 重链免疫球蛋白，其中所述修饰包括涉及与轻链接触的重链蛋白区域中的一个或多个突变; b）在促进所述修饰的人重链免疫球蛋白表达的条件下培养所述丝状宿主细胞; 和c）回收所述修饰的人重链免疫球蛋白。

    Hafnia Phytase Variants
    55.
    发明申请
    Hafnia Phytase Variants 有权
    Hafnia植酸酶变体

    公开(公告)号：US20120225468A1

    公开(公告)日：2012-09-06

    申请号：US13472822

    申请日：2012-05-16

    申请人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Lars Kobberoee Skov ,  Jesper Vind

    发明人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Lars Kobberoee Skov ,  Jesper Vind

    IPC分类号： C12N9/16

    CPC分类号： C12N9/16 ,  A23K20/189 ,  A23K50/30 ,  A23K50/75 ,  Y02E50/17

    摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

    摘要翻译： 本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。 这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度 简介 和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

    Hafnia phytase variants
    56.
    发明授权
    Hafnia phytase variants 有权
    Hafnia植酸酶变体

    公开(公告)号：US08206962B2

    公开(公告)日：2012-06-26

    申请号：US12568312

    申请日：2009-09-28

    申请人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Lars Kobberoee Skov ,  Jesper Vind

    发明人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Lars Kobberoee Skov ,  Jesper Vind

    IPC分类号： C12N9/14 ,  C12Q1/34 ,  C07H21/04

    CPC分类号： C12N9/16 ,  A23K20/189 ,  A23K50/30 ,  A23K50/75 ,  Y02E50/17

    摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

    摘要翻译： 本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。 这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度 简介 和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

    Synthase Inhibitor Screening Method
    58.
    发明申请
    Synthase Inhibitor Screening Method 审中-公开
    合成酶抑制剂筛选方法

    公开(公告)号：US20100267067A1

    公开(公告)日：2010-10-21

    申请号：US12743974

    申请日：2008-11-26

    申请人： Jesper Vind

    发明人： Jesper Vind

    IPC分类号： C12Q1/48 ,  C12Q1/02 ,  C12Q1/26 ,  C12Q1/34

    CPC分类号： C12Q1/02

    摘要： The present invention relates to a method for selecting at least one host cell secreting one or more active enzyme of interest, said method comprising the steps of: a) providing a growth medium comprising one or more synthase inhibitor, which inhibits the synthesis of at least one essential compound in the host cell, and further comprising one or more component, which in the presence of the one or more active enzyme of interest is converted into the at least one essential compound, thereby allowing the host cell to grow; b) cultivating the host cell in or on the growth medium of step (a); and c) selecting at least one host cell capable of growing in or on the growth medium of step (a), which host cell secretes one or more active enzyme of interest.

    摘要翻译： 本发明涉及一种选择至少一种分泌一种或多种感兴趣的活性酶的宿主细胞的方法，所述方法包括以下步骤：a）提供包含一种或多种合成酶抑制剂的生长培养基，其抑制至少 宿主细胞中的一种必需化合物，并且还包含一种或多种组分，其在一种或多种感兴趣的活性酶存在下转化为至少一种必需化合物，从而允许宿主细胞生长; b）在步骤（a）的生长培养基中或其上培养宿主细胞; 和c）选择能够在步骤（a）的生长培养基中或其上生长的至少一种宿主细胞，所述宿主细胞分泌一种或多种感兴趣的活性酶。

    Enzymatic Enantioselective Ester or Amide Hydrolysis or Synthesis
    59.
    发明申请
    Enzymatic Enantioselective Ester or Amide Hydrolysis or Synthesis 审中-公开
    酶对映选择性酯或酰胺水解或合成

    公开(公告)号：US20080138856A1

    公开(公告)日：2008-06-12

    申请号：US11813614

    申请日：2006-02-10

    申请人： Allan Svendsen ,  Jesper Vind ,  Jesper Brask ,  Leonardo De Maria

    发明人： Allan Svendsen ,  Jesper Vind ,  Jesper Brask ,  Leonardo De Maria

    IPC分类号： C12P21/06 ,  C12P7/62

    CPC分类号： C12N9/20 ,  C07K2299/00 ,  C12P7/02 ,  C12P7/40 ,  C12P7/62 ,  C12P13/02 ,  C12P41/004 ,  C12P41/007

    摘要： The enantioselectivity of fungal lipolytic enzymes can be altered by substituting a suitably selected amino acid residue. The residue to be substituted is selected from its location in the 3D structure of the enzyme and an ester substrate (or a substrate analogue). A residue in the lid may be selected if it is located close to the acid part or close to the alcohol part of an ester substrate. A residue outside the lid region may be selected if it is located close to the active site or close to the substrate.

    摘要翻译： 真菌脂肪分解酶的对映体选择性可以通过替代适当选择的氨基酸残基来改变。 待取代的残基选自其酶的位置和酯底物（或底物类似物）的3D结构。 如果位于靠近酸性部分或靠近酯基材的醇部分的位置，则可以选择盖子中的残余物。 如果位于靠近活性部位或靠近基底的位置，则可以选择盖子外部的残余物。